Abstract

We present the results of refractive index determination in dehydrated cells of human oral cavity epithelium by means of digital holographic microscopy. Statistical analysis of the obtained results provided the mean value of the refractive index of 1.478 (0.025). The measurement accuracy for refractive index and thickness in both living and dehydrated cells was estimated. As shown, the absolute error of holographic measurements of refractive index in living cells is more than an order of magnitude higher than that in dry cells. The results obtained demonstrate that monitoring of refractive index distributions in nonstained label-free biopsic or histological samples can be considered as a promising tool for the development of rapid diagnostics of pathological changes in cells and tissues.

© 2017 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy

Benjamin Rappaz, Pierre Marquet, Etienne Cuche, Yves Emery, Christian Depeursinge, and Pierre J. Magistretti
Opt. Express 13(23) 9361-9373 (2005)

Full-field optical coherence microscopy for identifying live cancer cells by quantitative measurement of refractive index distribution

Woo June Choi, Do In Jeon, Sang-Gun Ahn, Jung-Hoon Yoon, Sungho Kim, and Byeong Ha Lee
Opt. Express 18(22) 23285-23295 (2010)

Cell refractive index tomography by digital holographic microscopy

Florian Charrière, Anca Marian, Frédéric Montfort, Jonas Kuehn, Tristan Colomb, Etienne Cuche, Pierre Marquet, and Christian Depeursinge
Opt. Lett. 31(2) 178-180 (2006)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (7)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription