Abstract

The photodamage in coherent anti-Stokes Raman scattering (CARS) imaging of spinal tissues is featured by plasma-induced myelin splitting and shockwaves. When the excitation is tuned on resonance with the symmetric CH2 stretch vibration, the average point-scanning time to cause the photodamage is reduced by half. Similar Raman resonance-enhanced photodamage is also observed for a polymer film. The light–matter energy transfer in coherent Raman processes with both plane waves and focused excitation beams is analyzed to interpret this phenomenon. Our calculation indicates that at Raman resonance, a significant vibrational absorption in the material can be stimulated by the concomitant Raman gain and Raman loss processes due to high incident-field intensities under a tight-focusing condition. As a result, while the nonlinear damage induced by multiphoton absorption can be diminished in CARS microscopy owing to the use of near-infrared picosecond pulses, the coherent Raman-induced vibrational pumping is able to enhance the photodamage by assisting plasma generation in the material.

© 2007 Optical Society of America

Full Article  |  PDF Article
Related Articles
Characterization of photodamage in coherent anti-Stokes Raman scattering microscopy

Yan Fu, Haifeng Wang, Riyi Shi, and Ji-Xin Cheng
Opt. Express 14(9) 3942-3951 (2006)

Ex vivo and in vivo imaging of myelin fibers in mouse brain by coherent anti-Stokes Raman scattering microscopy

Yan Fu, T. Brandon Huff, Han-Wei Wang, Haifeng Wang, and Ji-Xin Cheng
Opt. Express 16(24) 19396-19409 (2008)

A multimodal platform for nonlinear optical microscopy and microspectroscopy

Hongtao Chen, Haifeng Wang, Mikhail N. Slipchenko, YooKyung Jung, Yunzhou Shi, Jiabin Zhu, Kimberly K. Buhman, and Ji-Xin Cheng
Opt. Express 17(3) 1282-1290 (2009)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (32)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription