Abstract

The luminescence of dye molecules depends on their position in a layered optical system. Conversely, the luminescence can be applied to measure the position of dye molecules above an interface. We formulate the electromagnetic theory of stationary fluorescence in a layered optical system—of light absorption, light detection, and fluorescence lifetime—computing the angular dependence of dipole interaction with all plane waves by a classical Sommerfeld approach. The theory is checked by experiments with stained lipid membranes on silicon with 256 terraces of silicon dioxide. We apply the electromagnetic theory to fluorescence micrographs of living cells on oxidized silicon chips and evaluate distances between the cell membrane and the substrate in a range of 1–150 nm.

© 2002 Optical Society of America

Full Article  |  PDF Article
Related Articles
Membrane imaging by second-harmonic generation microscopy

L. Moreaux, O. Sandre, and J. Mertz
J. Opt. Soc. Am. B 17(10) 1685-1694 (2000)

Fluorescence correlation spectroscopy in surface plasmon coupled emission microscope

J. Borejdo, N. Calander, Z. Gryczynski, and I. Gryczynski
Opt. Express 14(17) 7878-7888 (2006)

Orientational effects in the excitation and de-excitation of single molecules interacting with donut-mode laser beams

Peter Dedecker, Benoît Muls, Johan Hofkens, Jörg Enderlein, and Jun-ichi Hotta
Opt. Express 15(6) 3372-3383 (2007)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (12)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (113)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription