Abstract

Microscopic mapping of accumulated photon-echo relaxation time, known as accumulated photon-echo imaging, has been developed as a new technique accessible to histological diagnosis. Photon-echo imaging for Rhodamine-stained tissue samples of human liver has been demonstrated by means of fluorescence detection of accumulated photon echo. In a liver tissue that included a moderately differentiated hepatocellular carcinoma, we obtained an accumulated photon-echo image that correlated with the histological diagnosis. However, for a liver tissue with a highly differentiated hepatocellular carcinoma, photon-echo imaging could not discriminate the carcinoma from a noncancerous liver tissue.

© 1997 Optical Society of America

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