Abstract

An innovative fluorescence lifetime technique, called transient fluorescence spectroscopy, has been developed and applied toward the determination of the fluorescence lifetimes (a value near 1ms) of terbium doped dipicolinic acid (DPA-Tb). This different lifetime measurement technique uses a high-pulse-repetition-frequency (8kHz) pulsed UV laser excitation source and a slow modulated chopper (50to1000Hz) to produce the modulated emission intensity from the DPA-Tb, and compares these to a theoretical transient solution of the population rate equations for the DPA-Tb energy levels. Curve fitting of the transient solution of the modulated emission intensity to that obtained experimentally yielded a value for the fluorescence lifetime.

© 2009 Optical Society of America

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