Abstract

After analyzing the three-dimensional image formation in fiber-optical confocal scanning microscopes, a three-dimensional effective point-spread function is introduced. For both reflection-mode and transmission-mode microscopes of small circular aperture, analytical expressions for the three-dimensional coherent transfer functions are derived. The relation between the three-dimensional and the two-dimensional (for thin objects) coherent transfer functions in these microscopic systems is further investigated. Three-dimensional numerical plots of the coherent transfer functions reveal their dependence on the fiber spot size.

© 1991 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Image formation in a fiber-optical confocal scanning microscope

Min Gu, C. J. R. Sheppard, and X. Gan
J. Opt. Soc. Am. A 8(11) 1755-1761 (1991)

Three-dimensional phase imaging with the intensity transport equation

Colin J. R. Sheppard
Appl. Opt. 41(28) 5951-5955 (2002)

Three-dimensional coherent transfer function for a confocal microscope with two D-shaped pupils

Ke Si, Wei Gong, and Colin J. R. Sheppard
Appl. Opt. 48(5) 810-817 (2009)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (36)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription