Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
Journal Home About Tutorials Issues in Progress Current Issue All Issues Early Posting Feature Issues

Influence of high numerical aperture on depth-of-field enhancing phase mask optimization in localization microscopy

Olivier Lévêque, Romain Duverger, Hervé Sauer, Caroline Kulcsár, and François Goudail

Not Accessible

Your library or personal account may give you access

Abstract

The depth-of-field (DoF) of localization microscopes can be extended by placing a phase mask in the aperture stop of the objective. To optimize these masks and characterize their performance, defocus is in general modeled by a simple quadratic pupil phase term. However, this model does not take into account two essential characteristics of localization microscopy setups: an extremely high numerical aperture (NA) and a mismatch between the refractive indices of the immersion liquid and sample. Using the more realistic high NA image formation model of Gibson & Lanni (GL), we show that the DoF extension is simply reduced by a NA-dependent scaling factor. We also show that, provided this scaled DoF extension factor is taken into account, masks optimized with the approximate quadratic model are still nearly optimal in the framework of the GL model. This result is important since it establishes that generic optimized masks can be used in setups with different NA and immersion indices.

© 2021 Optical Society of America

Full Article  |  PDF Article

Data Availability

Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (7)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (2)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (38)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters
Select Topics Cancel
Top
       
© Copyright 2024 | Optica Publishing Group. All rights reserved, including rights for text and data mining and training of artificial technologies or similar technologies.
Privacy | Terms of Use