Abstract

We compare two recently developed multiple-frame deconvolution approaches for the reconstruction of structured illumination microscopy (SIM) data: the pattern-illuminated Fourier ptychography algorithm (piFP) and the joint Richardson–Lucy deconvolution (jRL). The quality of the images reconstructed by these methods is compared in terms of the achieved resolution improvement, noise enhancement, and inherent artifacts. Furthermore, we study the issue of object-dependent resolution improvement by considering the modulation transfer functions derived from different types of objects. The performance of the considered methods is tested in experiments and benchmarked with a commercial SIM microscope. We find that the piFP method resolves periodic and isolated structures equally well, whereas the jRL method provides significantly higher resolution for isolated objects compared to periodic ones. Images reconstructed by the piFP and jRL algorithms are comparable to the images reconstructed using the generalized Wiener filter applied in most commercial SIM microscopes. An advantage of the discussed algorithms is that they allow the reconstruction of SIM images acquired under different types of illumination, such as multi-spot or random illumination.

© 2016 Optical Society of America

Full Article  |  PDF Article

Corrections

11 May 2016: A correction was made to the text on pages B14 and B19.


OSA Recommended Articles
Studying different illumination patterns for resolution improvement in fluorescence microscopy

Nadya Chakrova, Rainer Heintzmann, Bernd Rieger, and Sjoerd Stallinga
Opt. Express 23(24) 31367-31383 (2015)

Structured illumination microscopy with unknown patterns and a statistical prior

Li-Hao Yeh, Lei Tian, and Laura Waller
Biomed. Opt. Express 8(2) 695-711 (2017)

Image reconstruction for structured-illumination microscopy with low signal level

Kaiqin Chu, Paul J. McMillan, Zachary J. Smith, Jie Yin, Jeniffer Atkins, Paul Goodwin, Sebastian Wachsmann-Hogiu, and Stephen Lane
Opt. Express 22(7) 8687-8702 (2014)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (7)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (17)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription