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Imaging interferometric microscopy

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Abstract

Imaging interferometric microscopy (IIM) is a synthetic aperture imaging approach providing resolution to the transmission medium (refractive index n) linear systems limit extending to λ4n using only low-numerical-aperture (low-NA) optics. IIM uses off-axis illumination to access high spatial frequencies along with interferometric reintroduction of a zero-order reference beam on the low-NA side of the optical system. For a thin object normal to the optical axis, the frequency space limit is [(1+NA)nλ], while tilting the object plane allows collection of diffraction information up to the material transmission bandpass-limited spatial frequency of 2nλ. Tilting transforms the spatial frequencies; the algorithm to reset to the correct image frequencies is described. IIM involves combining multiple subimages; the image reconstruction procedures are discussed. A mean-square-error metric is introduced. For binary objects, sigmoidal filtering of the image provides significant resolution improvement.

© 2008 Optical Society of America

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