Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Theoretical investigation of the photon efficiency in frequency-domain fluorescence lifetime imaging microscopy

Not Accessible

Your library or personal account may give you access

Abstract

We investigate the photon efficiency of frequency-domain fluorescence lifetime imaging microscopy, using both theoretical and Monte Carlo methods. Our analysis differs from previous work in that it incorporates the data fitting process used in real experiments, allows for the arbitrary choice of excitation and gain waveforms, and calculates lifetimes as well as associated F-values from higher harmonics in the data. Using our analysis, we found different photon efficiencies to those previously reported and were able to propose optimal excitation and gain waveforms. Additionally, we suggest measurement protocols that lead to further improvement in photon efficiency. We compare our results to other techniques for lifetime imaging and consider the implications of our higher-harmonic analysis for multi-exponential lifetime determination.

© 2008 Optical Society of America

Full Article  |  PDF Article
More Like This
Investigation of signal-to-noise ratio in frequency-domain multiphoton fluorescence lifetime imaging microscopy

Yide Zhang, Aamir A. Khan, Genevieve D. Vigil, and Scott S. Howard
J. Opt. Soc. Am. A 33(7) B1-B11 (2016)

Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging

Johan Philip and Kjell Carlsson
J. Opt. Soc. Am. A 20(2) 368-379 (2003)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (11)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (48)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved