Abstract

Spectral curves of direct transmittance, diffuse transmittance, and scattering by partial layers of oriented red blood cells were measured. Also determined were the average optical parameters of a single cell and the cell concentration in the partial layers. The experiments are an extension and refinement of those of Lothian and Lewis on red blood cells and of Latimer on the green alga Chlorella. By assuming large-particle optics, equations were derived to predict the spectral curves in terms of cell and layer parameters. Experimental and theoretical curves are in reasonable agreement.

PDF Article

References

  • View by:
  • |
  • |

  1. E. E. Jacobs, A. S. Holt, R. Kromhout, and E. Rabinowitch, Arch. Biochem. Biophys. 72, 495 (1957). See also E. Rabinowitch, Photosynthesis and Related Processes (Interscience Publishers, Inc., New York, 1956), Vol. II, Part 2, p. 1816.
  2. S. Anderson, J. Opt. Soc. Am. 39, 49 (1949).
  3. J. W. Weigl, J. Chem. Phys. 24, 577 (1956).
  4. P. Latimer and E. Rabinowitch, Arch. Biochem. Biophys. 84, 428 (1959).
  5. P. Latimer, Plant Physiol. 34, 193 (1959). See also, P. Latimer, Science 127, 29 (1958).
  6. F. S. Brackett and E. Charney, J. Opt. Soc. Am. 50, 811 (1960). See also E. Charney and F. Brackett, Arch. Biochem. Biophys. 92, 1 (1961).
  7. G. F. Lothian and P. C. Lewis, Nature 178, 1342 (1956). See also G. F. Lothian, Absorption Spectrophotometry (The Mac-Millan Company, New York, 1958), p. 35.
  8. P. Latimer, thesis, University of Illinois, Urbana, Illinois (1956).
  9. K. Shibata, A. A. Benson, and M. Calvin, Biochim. et Biophys. Acta 15, 461 (1954).
  10. S. Keilin and E. F. Hartree, Biochim. et Biophys. Acta 27, 173 (1958).
  11. M. Born and E. Wolf, Principles of Optics (Pergamon Press, New York, 1959), p. 395.
  12. H. C. van de Hulst, Light Scattering by Small Particles (John Wiley & Sons, Inc., New York, 1957), p. 107 and 193.
  13. P. Latimer, J. Opt. Soc. Am. 51, 116 (1961).
  14. M. M. Wintrobe, Clinical Hematology (Lea & Febiger Publishers, Philadelphia, Pennsylvania, 1951), 3rd ed., p. 312.
  15. R. Barer, Physical Techniques in Biological Research, III, edited by G. Oster and A. W. Pollister (Academic Press, Inc., New York, 1956), p. 29.
  16. D. L. Drabkin and J. H. Austin, J. Biol. Chem. 112, 51 (1951).
  17. R. Barer and S. Joseph, Quart. J. Microscop. Sci. 95, 399 (1954).

Anderson, S.

S. Anderson, J. Opt. Soc. Am. 39, 49 (1949).

Austin, J. H.

D. L. Drabkin and J. H. Austin, J. Biol. Chem. 112, 51 (1951).

Barer, R.

R. Barer and S. Joseph, Quart. J. Microscop. Sci. 95, 399 (1954).

R. Barer, Physical Techniques in Biological Research, III, edited by G. Oster and A. W. Pollister (Academic Press, Inc., New York, 1956), p. 29.

Benson, A. A.

K. Shibata, A. A. Benson, and M. Calvin, Biochim. et Biophys. Acta 15, 461 (1954).

Born, M.

M. Born and E. Wolf, Principles of Optics (Pergamon Press, New York, 1959), p. 395.

Brackett, F. S.

F. S. Brackett and E. Charney, J. Opt. Soc. Am. 50, 811 (1960). See also E. Charney and F. Brackett, Arch. Biochem. Biophys. 92, 1 (1961).

Calvin, M.

K. Shibata, A. A. Benson, and M. Calvin, Biochim. et Biophys. Acta 15, 461 (1954).

Charney, E.

F. S. Brackett and E. Charney, J. Opt. Soc. Am. 50, 811 (1960). See also E. Charney and F. Brackett, Arch. Biochem. Biophys. 92, 1 (1961).

Drabkin, D. L.

D. L. Drabkin and J. H. Austin, J. Biol. Chem. 112, 51 (1951).

Hartree, E. F.

S. Keilin and E. F. Hartree, Biochim. et Biophys. Acta 27, 173 (1958).

Holt, A. S.

E. E. Jacobs, A. S. Holt, R. Kromhout, and E. Rabinowitch, Arch. Biochem. Biophys. 72, 495 (1957). See also E. Rabinowitch, Photosynthesis and Related Processes (Interscience Publishers, Inc., New York, 1956), Vol. II, Part 2, p. 1816.

Jacobs, E. E.

E. E. Jacobs, A. S. Holt, R. Kromhout, and E. Rabinowitch, Arch. Biochem. Biophys. 72, 495 (1957). See also E. Rabinowitch, Photosynthesis and Related Processes (Interscience Publishers, Inc., New York, 1956), Vol. II, Part 2, p. 1816.

Joseph, S.

R. Barer and S. Joseph, Quart. J. Microscop. Sci. 95, 399 (1954).

Keilin, S.

S. Keilin and E. F. Hartree, Biochim. et Biophys. Acta 27, 173 (1958).

Kromhout, R.

E. E. Jacobs, A. S. Holt, R. Kromhout, and E. Rabinowitch, Arch. Biochem. Biophys. 72, 495 (1957). See also E. Rabinowitch, Photosynthesis and Related Processes (Interscience Publishers, Inc., New York, 1956), Vol. II, Part 2, p. 1816.

Latimer, P.

P. Latimer, Plant Physiol. 34, 193 (1959). See also, P. Latimer, Science 127, 29 (1958).

P. Latimer and E. Rabinowitch, Arch. Biochem. Biophys. 84, 428 (1959).

P. Latimer, thesis, University of Illinois, Urbana, Illinois (1956).

P. Latimer, J. Opt. Soc. Am. 51, 116 (1961).

Lewis, P. C.

G. F. Lothian and P. C. Lewis, Nature 178, 1342 (1956). See also G. F. Lothian, Absorption Spectrophotometry (The Mac-Millan Company, New York, 1958), p. 35.

Lothian, G. F.

G. F. Lothian and P. C. Lewis, Nature 178, 1342 (1956). See also G. F. Lothian, Absorption Spectrophotometry (The Mac-Millan Company, New York, 1958), p. 35.

Rabinowitch, E.

P. Latimer and E. Rabinowitch, Arch. Biochem. Biophys. 84, 428 (1959).

E. E. Jacobs, A. S. Holt, R. Kromhout, and E. Rabinowitch, Arch. Biochem. Biophys. 72, 495 (1957). See also E. Rabinowitch, Photosynthesis and Related Processes (Interscience Publishers, Inc., New York, 1956), Vol. II, Part 2, p. 1816.

Shibata, K.

K. Shibata, A. A. Benson, and M. Calvin, Biochim. et Biophys. Acta 15, 461 (1954).

van de Hulst, H. C.

H. C. van de Hulst, Light Scattering by Small Particles (John Wiley & Sons, Inc., New York, 1957), p. 107 and 193.

Weigl, J. W.

J. W. Weigl, J. Chem. Phys. 24, 577 (1956).

Wintrobe, M. M.

M. M. Wintrobe, Clinical Hematology (Lea & Febiger Publishers, Philadelphia, Pennsylvania, 1951), 3rd ed., p. 312.

Wolf, E.

M. Born and E. Wolf, Principles of Optics (Pergamon Press, New York, 1959), p. 395.

Other

E. E. Jacobs, A. S. Holt, R. Kromhout, and E. Rabinowitch, Arch. Biochem. Biophys. 72, 495 (1957). See also E. Rabinowitch, Photosynthesis and Related Processes (Interscience Publishers, Inc., New York, 1956), Vol. II, Part 2, p. 1816.

S. Anderson, J. Opt. Soc. Am. 39, 49 (1949).

J. W. Weigl, J. Chem. Phys. 24, 577 (1956).

P. Latimer and E. Rabinowitch, Arch. Biochem. Biophys. 84, 428 (1959).

P. Latimer, Plant Physiol. 34, 193 (1959). See also, P. Latimer, Science 127, 29 (1958).

F. S. Brackett and E. Charney, J. Opt. Soc. Am. 50, 811 (1960). See also E. Charney and F. Brackett, Arch. Biochem. Biophys. 92, 1 (1961).

G. F. Lothian and P. C. Lewis, Nature 178, 1342 (1956). See also G. F. Lothian, Absorption Spectrophotometry (The Mac-Millan Company, New York, 1958), p. 35.

P. Latimer, thesis, University of Illinois, Urbana, Illinois (1956).

K. Shibata, A. A. Benson, and M. Calvin, Biochim. et Biophys. Acta 15, 461 (1954).

S. Keilin and E. F. Hartree, Biochim. et Biophys. Acta 27, 173 (1958).

M. Born and E. Wolf, Principles of Optics (Pergamon Press, New York, 1959), p. 395.

H. C. van de Hulst, Light Scattering by Small Particles (John Wiley & Sons, Inc., New York, 1957), p. 107 and 193.

P. Latimer, J. Opt. Soc. Am. 51, 116 (1961).

M. M. Wintrobe, Clinical Hematology (Lea & Febiger Publishers, Philadelphia, Pennsylvania, 1951), 3rd ed., p. 312.

R. Barer, Physical Techniques in Biological Research, III, edited by G. Oster and A. W. Pollister (Academic Press, Inc., New York, 1956), p. 29.

D. L. Drabkin and J. H. Austin, J. Biol. Chem. 112, 51 (1951).

R. Barer and S. Joseph, Quart. J. Microscop. Sci. 95, 399 (1954).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.