Abstract

A reflecting microscope designed for use in front of the entrance slit of a Perkin-Elmer single-beam infrared spectrometer is described. It is shown that the heating effect of the condensed beam on the sample is not significant except in the case of compounds which absorb strongly in the 3-micron region of the spectrum, and then only if the sample is unsupported. The instrument is being applied to the study of small quantities of material in solution and to single crystals. Spectra adequate for identification purposes may be obtained from less than 10 micrograms of a sample in solution. The minimum sample size is limited at present by the technique of sample transfer and if this can be improved, spectra could be obtained with about 1 microgram. Preliminary results using polarized radiation in the study of fatty acid crystals are also presented.

© 1952 Optical Society of America

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References

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  1. Barer, Cole, and Thompson, Nature 163, 198 (1949).
    [CrossRef] [PubMed]
  2. C. R. Burch, Proc. Phys. Soc. (London) 59, 41 (1947).
    [CrossRef]
  3. D. L. Wood, Rev. Sci. Instr. 21, 764 (1950).
    [CrossRef]
  4. Norris, Seeds, and Wilkins, J. Opt. Soc. Am. 41, 111 (1951).
    [CrossRef]
  5. R. C. Gore, Science 110, 710 (1949).
    [CrossRef]
  6. Blout, Bird, and Grey, J. Opt. Soc. Am. 39, 1052 (1949); J. Opt. Soc. Am. 40, 304 (1950).
  7. E. H. Land and et al., Science 109, 371 (1949).
    [CrossRef] [PubMed]
  8. B. K. Johnson, J. Sci. Instr. 26, 148 (1949).
    [CrossRef]
  9. D. S. Grey and P. H. Lee, J. Opt. Soc. Am. 39, 719 (1949).
    [CrossRef] [PubMed]
  10. D. S. Grey, J. Opt. Soc. Am. 39, 723 (1949).
    [CrossRef] [PubMed]
  11. K. P. Norris and M. H. F. Wilkins, Trans. Faraday Soc. Discussions 9, 360 (1950).
    [CrossRef]
  12. K. Schwarzschild, Theorie der Spiegelteleskope (Göttingen Observatory, 1905).
  13. Perkin-Elmer Corporation, Instr. News 2, No. 3, 8 (1951).
  14. Jones, McKay, and Sinclair, J. Am. Chem. Soc. (to be published).
  15. G. B. B. M. Sutherland, Trans. Faraday Soc. Disc. 9, 279 (1950).
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    [CrossRef]
  17. E. R. Blout and G. R. Bird, J. Opt. Soc. 41, 547 (1951).
    [CrossRef]

1951 (3)

Perkin-Elmer Corporation, Instr. News 2, No. 3, 8 (1951).

E. R. Blout and G. R. Bird, J. Opt. Soc. 41, 547 (1951).
[CrossRef]

Norris, Seeds, and Wilkins, J. Opt. Soc. Am. 41, 111 (1951).
[CrossRef]

1950 (3)

K. P. Norris and M. H. F. Wilkins, Trans. Faraday Soc. Discussions 9, 360 (1950).
[CrossRef]

G. B. B. M. Sutherland, Trans. Faraday Soc. Disc. 9, 279 (1950).

D. L. Wood, Rev. Sci. Instr. 21, 764 (1950).
[CrossRef]

1949 (7)

1947 (1)

C. R. Burch, Proc. Phys. Soc. (London) 59, 41 (1947).
[CrossRef]

Barer,

Barer, Cole, and Thompson, Nature 163, 198 (1949).
[CrossRef] [PubMed]

Bird,

Bird, G. R.

E. R. Blout and G. R. Bird, J. Opt. Soc. 41, 547 (1951).
[CrossRef]

Blout,

Blout, E. R.

E. R. Blout and G. R. Bird, J. Opt. Soc. 41, 547 (1951).
[CrossRef]

Burch, C. R.

C. R. Burch, Proc. Phys. Soc. (London) 59, 41 (1947).
[CrossRef]

Cole,

Barer, Cole, and Thompson, Nature 163, 198 (1949).
[CrossRef] [PubMed]

Dobriner, K.

R. N. Jones and K. Dobriner, Vitamins and Hormones (Academic Press, Inc., New York, 1949), Vol. 7, pp. 293–363.
[CrossRef]

Gore, R. C.

R. C. Gore, Science 110, 710 (1949).
[CrossRef]

Grey,

Grey, D. S.

Johnson, B. K.

B. K. Johnson, J. Sci. Instr. 26, 148 (1949).
[CrossRef]

Jones,

Jones, McKay, and Sinclair, J. Am. Chem. Soc. (to be published).

Jones, R. N.

R. N. Jones and K. Dobriner, Vitamins and Hormones (Academic Press, Inc., New York, 1949), Vol. 7, pp. 293–363.
[CrossRef]

Land, E. H.

E. H. Land and et al., Science 109, 371 (1949).
[CrossRef] [PubMed]

Lee, P. H.

McKay,

Jones, McKay, and Sinclair, J. Am. Chem. Soc. (to be published).

Norris,

Norris, K. P.

K. P. Norris and M. H. F. Wilkins, Trans. Faraday Soc. Discussions 9, 360 (1950).
[CrossRef]

Schwarzschild, K.

K. Schwarzschild, Theorie der Spiegelteleskope (Göttingen Observatory, 1905).

Seeds,

Sinclair,

Jones, McKay, and Sinclair, J. Am. Chem. Soc. (to be published).

Sutherland, G. B. B. M.

G. B. B. M. Sutherland, Trans. Faraday Soc. Disc. 9, 279 (1950).

Thompson,

Barer, Cole, and Thompson, Nature 163, 198 (1949).
[CrossRef] [PubMed]

Wilkins,

Wilkins, M. H. F.

K. P. Norris and M. H. F. Wilkins, Trans. Faraday Soc. Discussions 9, 360 (1950).
[CrossRef]

Wood, D. L.

D. L. Wood, Rev. Sci. Instr. 21, 764 (1950).
[CrossRef]

J. Opt. Soc. (1)

E. R. Blout and G. R. Bird, J. Opt. Soc. 41, 547 (1951).
[CrossRef]

J. Opt. Soc. Am. (4)

J. Sci. Instr. (1)

B. K. Johnson, J. Sci. Instr. 26, 148 (1949).
[CrossRef]

Nature (1)

Barer, Cole, and Thompson, Nature 163, 198 (1949).
[CrossRef] [PubMed]

Perkin-Elmer Corporation, Instr. News (1)

Perkin-Elmer Corporation, Instr. News 2, No. 3, 8 (1951).

Proc. Phys. Soc. (London) (1)

C. R. Burch, Proc. Phys. Soc. (London) 59, 41 (1947).
[CrossRef]

Rev. Sci. Instr. (1)

D. L. Wood, Rev. Sci. Instr. 21, 764 (1950).
[CrossRef]

Science (2)

R. C. Gore, Science 110, 710 (1949).
[CrossRef]

E. H. Land and et al., Science 109, 371 (1949).
[CrossRef] [PubMed]

Trans. Faraday Soc. Disc. (1)

G. B. B. M. Sutherland, Trans. Faraday Soc. Disc. 9, 279 (1950).

Trans. Faraday Soc. Discussions (1)

K. P. Norris and M. H. F. Wilkins, Trans. Faraday Soc. Discussions 9, 360 (1950).
[CrossRef]

Other (3)

K. Schwarzschild, Theorie der Spiegelteleskope (Göttingen Observatory, 1905).

R. N. Jones and K. Dobriner, Vitamins and Hormones (Academic Press, Inc., New York, 1949), Vol. 7, pp. 293–363.
[CrossRef]

Jones, McKay, and Sinclair, J. Am. Chem. Soc. (to be published).

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Figures (6)

Fig. 1
Fig. 1

Details of construction of the microscope.

Fig. 2
Fig. 2

Front view of completed microscope.

Fig. 3
Fig. 3

The microscope at the spectrometer with silver chloride transmission polarizer in position. The top of the spherical collecting mirror may be seen beyond the first housing.

Fig. 4
Fig. 4

Infrared spectrum of eicosanoic acid. (A) Solid film prepared by melting between sodium chloride plates. (B) Single crystal using reflecting microscope. (C) Same crystal as in (B), using polarized radiation.

Fig. 5
Fig. 5

Micro-absorption cell for small quantities of solution.

Fig. 6
Fig. 6

Infrared spectrum of androsterone in carbon disulfide solution. (A) Conventional macrocell. (B) Microcell as in Fig. 5 with reflecting microscope.

Tables (2)

Tables Icon

Table I Dimensions of reflecting microscope mirrors.

Tables Icon

Table II The heating effect using a globar source with the reflecting microscope.

Equations (1)

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P ( 10 μ ) = 8.5 × 10 - 5 .