Abstract

Microspectroscopy involves the combination of microscope and spectroscope optics for the purpose of investigating the spectra of small samples. The general methods of microspectroscopy are described as applied to absorption measurements in the ultraviolet, visible, and infra-red regions, emission spectroscopy, and other applications. Recently developed components and systems for microspectroscopy are emphasized, and some of the important requirements for their effective design and use are considered briefly.

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  1. T. Caspersson, Skand. Arch. f. Physiol., Supplement 73 (1936).
  2. J. F. Scott and R. L. Sinsheimer, "Ultraviolet microscopy and microspectroscopy," in Medical Physics (Year Book Publishers, Inc., Chicago, in press), Vol. 2.
  3. A. Kohler, Zeits. f. wiss. Mik. 21, 129, 273 (1904).
  4. B. K. Johnson, Proc. Phys. Soc., London 51, 1034 (1939).
  5. L. V. Foster and E. M. Thiel, J. Opt. Soc. Am. 38, 689 (1948); L. V. Foster, Analyt. Chem. 21, 432 (1949).
  6. E. M. Brumberg, Bull. Acad. Sci. U.S.S.R. 23, 486 (1941); 24, 33 (1942); Nature 152, 357 (1943).
  7. D. S. Grey and P. Lee, J. Opt. Soc. Am. 39, 719 (1949); D. S. Grey, J. Opt. Soc. Am. 39, 723 (1949).
  8. A. J. Kavanaugh (personal communication).
  9. W. E. Leeds and M. H. F. Wilkins, Nature 164, 228 (1949).
  10. C. R. Burch, Nature 152, 748 (1943); Proc. Phys. Soc., London 59, 41 (1947).
  11. T. Dunham, Jr., "The effect of central stops on the resolution of a microscope, I," Special Report No. 2 to the American Cancer Society (July, 1948).
  12. F. Sjostrand, "Über die eigenfluoreszenz tierischer gewebe mit besonderer Berucksichtigung der sangetierniere," Stockholm (1944).
  13. H. W. Wyckoff, Quarterly Progress Report, M.I.T. Research Laboratory of Electronics (July, 1949).
  14. T. Dunham, Jr., see J. R. Loofbourow, Growth Symposium 12, 75 (1948).
  15. Harrison, Lord, and Loofbourow, Practical Spectroscopy (Prentice-Hall, Inc., New York, 1948).
  16. P. A. Cole and E. Sutton, Cold Spring Harbor Symposia on Quantitative Biology 9, 66 (1941).
  17. E. W. Dempsey et al., Anat. Rev. 98, 417 (1947); see also R. E. Stowell, J. Nat. Cancer Inst. 3, 111 (1942); M. A. Lessler and H. A. Charipper, Science 110, 429 (1949).
  18. A. W. Pollister and H. Ris, Cold Spring Harbor Symposia on Quantitative Biology 12, 147 (1947).
  19. R. C. Mellors, Science (to be published); Mellors, Keane, and Streim, Nature (to be published).
  20. Land, Blout, Grey, Flower, Husek, Jones, Matz, and Merrill Science 109, 371 (1949).
  21. R. L. Sinsheimer, Ph.D. thesis, M.I.T. (1948).
  22. W. E. Forsythe, editor, Measurement of Radiant Energy (McGraw-Hill Book Company, Inc., New York, 1937).
  23. P. A. Cole and F. S. Brackett, J. Opt. Soc. Am. 11, 419 (1940).
  24. Barer, Cole, and Thompson, Nature 163, 198 (1949).
  25. Blout, Bird, and Grey, J. Opt. Soc. Am. 39, 1052 (1949).
  26. R. C. Gore, Science 110, 710 (1949).

Brackett, F. S.

P. A. Cole and F. S. Brackett, J. Opt. Soc. Am. 11, 419 (1940).

Brumberg, E. M.

E. M. Brumberg, Bull. Acad. Sci. U.S.S.R. 23, 486 (1941); 24, 33 (1942); Nature 152, 357 (1943).

Burch, C. R.

C. R. Burch, Nature 152, 748 (1943); Proc. Phys. Soc., London 59, 41 (1947).

Caspersson, T.

T. Caspersson, Skand. Arch. f. Physiol., Supplement 73 (1936).

Cole, P. A.

P. A. Cole and E. Sutton, Cold Spring Harbor Symposia on Quantitative Biology 9, 66 (1941).

P. A. Cole and F. S. Brackett, J. Opt. Soc. Am. 11, 419 (1940).

Dempsey, E. W.

E. W. Dempsey et al., Anat. Rev. 98, 417 (1947); see also R. E. Stowell, J. Nat. Cancer Inst. 3, 111 (1942); M. A. Lessler and H. A. Charipper, Science 110, 429 (1949).

Dunham, Jr., T.

T. Dunham, Jr., see J. R. Loofbourow, Growth Symposium 12, 75 (1948).

T. Dunham, Jr., "The effect of central stops on the resolution of a microscope, I," Special Report No. 2 to the American Cancer Society (July, 1948).

Forsythe, W. E.

W. E. Forsythe, editor, Measurement of Radiant Energy (McGraw-Hill Book Company, Inc., New York, 1937).

Foster, L. V.

L. V. Foster and E. M. Thiel, J. Opt. Soc. Am. 38, 689 (1948); L. V. Foster, Analyt. Chem. 21, 432 (1949).

Gore, R. C.

R. C. Gore, Science 110, 710 (1949).

Grey, D. S.

D. S. Grey and P. Lee, J. Opt. Soc. Am. 39, 719 (1949); D. S. Grey, J. Opt. Soc. Am. 39, 723 (1949).

Johnson, B. K.

B. K. Johnson, Proc. Phys. Soc., London 51, 1034 (1939).

Kavanaugh, A. J.

A. J. Kavanaugh (personal communication).

Kohler, A.

A. Kohler, Zeits. f. wiss. Mik. 21, 129, 273 (1904).

Lee, P.

D. S. Grey and P. Lee, J. Opt. Soc. Am. 39, 719 (1949); D. S. Grey, J. Opt. Soc. Am. 39, 723 (1949).

Leeds, W. E.

W. E. Leeds and M. H. F. Wilkins, Nature 164, 228 (1949).

Mellors, R. C.

R. C. Mellors, Science (to be published); Mellors, Keane, and Streim, Nature (to be published).

Pollister, A. W.

A. W. Pollister and H. Ris, Cold Spring Harbor Symposia on Quantitative Biology 12, 147 (1947).

Ris, H.

A. W. Pollister and H. Ris, Cold Spring Harbor Symposia on Quantitative Biology 12, 147 (1947).

Scott, J. F.

J. F. Scott and R. L. Sinsheimer, "Ultraviolet microscopy and microspectroscopy," in Medical Physics (Year Book Publishers, Inc., Chicago, in press), Vol. 2.

Sinsheimer, R. L.

J. F. Scott and R. L. Sinsheimer, "Ultraviolet microscopy and microspectroscopy," in Medical Physics (Year Book Publishers, Inc., Chicago, in press), Vol. 2.

R. L. Sinsheimer, Ph.D. thesis, M.I.T. (1948).

Sjostrand, F.

F. Sjostrand, "Über die eigenfluoreszenz tierischer gewebe mit besonderer Berucksichtigung der sangetierniere," Stockholm (1944).

Sutton, E.

P. A. Cole and E. Sutton, Cold Spring Harbor Symposia on Quantitative Biology 9, 66 (1941).

Thiel, E. M.

L. V. Foster and E. M. Thiel, J. Opt. Soc. Am. 38, 689 (1948); L. V. Foster, Analyt. Chem. 21, 432 (1949).

Wilkins, M. H. F.

W. E. Leeds and M. H. F. Wilkins, Nature 164, 228 (1949).

Wyckoff, H. W.

H. W. Wyckoff, Quarterly Progress Report, M.I.T. Research Laboratory of Electronics (July, 1949).

Other (26)

T. Caspersson, Skand. Arch. f. Physiol., Supplement 73 (1936).

J. F. Scott and R. L. Sinsheimer, "Ultraviolet microscopy and microspectroscopy," in Medical Physics (Year Book Publishers, Inc., Chicago, in press), Vol. 2.

A. Kohler, Zeits. f. wiss. Mik. 21, 129, 273 (1904).

B. K. Johnson, Proc. Phys. Soc., London 51, 1034 (1939).

L. V. Foster and E. M. Thiel, J. Opt. Soc. Am. 38, 689 (1948); L. V. Foster, Analyt. Chem. 21, 432 (1949).

E. M. Brumberg, Bull. Acad. Sci. U.S.S.R. 23, 486 (1941); 24, 33 (1942); Nature 152, 357 (1943).

D. S. Grey and P. Lee, J. Opt. Soc. Am. 39, 719 (1949); D. S. Grey, J. Opt. Soc. Am. 39, 723 (1949).

A. J. Kavanaugh (personal communication).

W. E. Leeds and M. H. F. Wilkins, Nature 164, 228 (1949).

C. R. Burch, Nature 152, 748 (1943); Proc. Phys. Soc., London 59, 41 (1947).

T. Dunham, Jr., "The effect of central stops on the resolution of a microscope, I," Special Report No. 2 to the American Cancer Society (July, 1948).

F. Sjostrand, "Über die eigenfluoreszenz tierischer gewebe mit besonderer Berucksichtigung der sangetierniere," Stockholm (1944).

H. W. Wyckoff, Quarterly Progress Report, M.I.T. Research Laboratory of Electronics (July, 1949).

T. Dunham, Jr., see J. R. Loofbourow, Growth Symposium 12, 75 (1948).

Harrison, Lord, and Loofbourow, Practical Spectroscopy (Prentice-Hall, Inc., New York, 1948).

P. A. Cole and E. Sutton, Cold Spring Harbor Symposia on Quantitative Biology 9, 66 (1941).

E. W. Dempsey et al., Anat. Rev. 98, 417 (1947); see also R. E. Stowell, J. Nat. Cancer Inst. 3, 111 (1942); M. A. Lessler and H. A. Charipper, Science 110, 429 (1949).

A. W. Pollister and H. Ris, Cold Spring Harbor Symposia on Quantitative Biology 12, 147 (1947).

R. C. Mellors, Science (to be published); Mellors, Keane, and Streim, Nature (to be published).

Land, Blout, Grey, Flower, Husek, Jones, Matz, and Merrill Science 109, 371 (1949).

R. L. Sinsheimer, Ph.D. thesis, M.I.T. (1948).

W. E. Forsythe, editor, Measurement of Radiant Energy (McGraw-Hill Book Company, Inc., New York, 1937).

P. A. Cole and F. S. Brackett, J. Opt. Soc. Am. 11, 419 (1940).

Barer, Cole, and Thompson, Nature 163, 198 (1949).

Blout, Bird, and Grey, J. Opt. Soc. Am. 39, 1052 (1949).

R. C. Gore, Science 110, 710 (1949).

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