Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Photoelectric Light-Scattering Photometer for Determining High Molecular Weights

Not Accessible

Your library or personal account may give you access

Abstract

A photoelectric photometer designed for the measurement of absolute turbidity, dissymmetry, and depolarization of dilute solutions of high molecular weight materials, and hence determination of their molecular weights, is described. The photometer comprises essentially a monochromatic parallel primary beam of radiation, a six-sided scattering cell for measurements at 0°, 45°, 90°, and 135°, a multiplier phototube and galvanometer, a standard opal glass diffusor, and removable polarizer and analyzer. Turbidity is determined in terms of a ratio of deflections for the 90° scattering and for the primary beam reduced in intensity by neutral filters and diffused by an opal glass plate. Working relationships leading to determination of absolute turbidity are developed. These relationships include corrections for refraction and reflection effects, and for imperfect diffusion by the opal glass. The latter is evaluated by comparison of the opal glass with reflecting diffusors corrected for specular component of reflectance. The response of some multiplier photo-tubes is shown to be dependent on the plane of polarization of the incident radiation. Data illustrating the performance of the photometer include comparison of molecular weights of polystyrene fractions, beta-lactoglobulin, bovine serum albumin, lysozyme, sucrose octaacetate, Raleigh’s ratio and depolarization for benzene, turbidity of a “standard” polystyrene, and particle size of a GR-S latex, with data obtained by other methods or other investigators.

© 1950 Optical Society of America

Full Article  |  PDF Article
More Like This
A Design for an Absolute Light-Scattering Photometer

Lawrence M. Kushner
J. Opt. Soc. Am. 44(2) 155-159 (1954)

Automatic Photometer for Measuring the Angular Dissymmetry of Light Scattering*

F. J. Baum and F. W. Billmeyer
J. Opt. Soc. Am. 51(4) 452-456 (1961)

Measurement of Photographic Transmission Density*

K. S. Weaver
J. Opt. Soc. Am. 40(8) 524-536 (1950)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (7)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (6)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (26)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved