Abstract

This spectrophotometer was designed specifically to measure in the visible region the absorption spectrum of visual purple, the photosensitive substance of the retinal rods. It is independent of both the photoelectric cell and amplifier characteristics, relying only on the law of crossed polarizing prisms. The sensitivity of the detecting system is high enough so that the measuring light produces no detectable effect upon the measured solution. The entire system can measure a transmission of 1 percent to a precision of about 1 percent.

© 1938 Optical Society of America

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