Abstract

A novel fiber optofluidic laser biosensor based on nanoparticle-functionalized thin-walled hollow optical fiber is developed for sensitive detection of enzyme horseradish peroxidase (HRP). High sensitivity is achieved thanks to the high surface-to-volume ratio of the nanomaterials. Two kinds of attractive nanomaterials, i.e., Au nanorods and SiO₂ nanoparticles, are selected as demonstrative models to be coated onto the inner surface of thin-walled hollow fiber through electrostatic adsorption and biochemical cross-linking, respectively, while the streptavidin-biotin conjugation mechanism acts as a bridge for the specific recognition of enzyme. The nanoparticle-functionalized fiber optofluidic lasers have lower lasing threshold, compared to the control group without nanomaterial. We experimentally demonstrate that the nanomaterials enhanced fiber optofluidic laser biosensors can detect HRP in the range of 75–15 000 pM with a good linearity in semi-logarithmic scale, and with a sensitivity enhancement of 57.4% by using the SiO₂ nanoparticles. We believe that this nanoparticle-enhanced fiber optofluidic laser biosensor could provide an efficient strategy for biomolecule detection such as enzyme-linked immunosorbent assay (ELISA).

Signal-enhanced multi-core fiber-based WaveFlex biosensor for ultra-sensitive xanthine detection

Qianqian Fu, Yiyan Xie, Feilong Gao, Wenxu Zhu, Xianzheng Lang, Ragini Singh, Bingyuan Zhang, and Santosh Kumar
Opt. Express 31(26) 43178-43197 (2023)

Highly sensitive and label-free detection of biotin using a liquid crystal-based optofluidic biosensor

Haonan Wang, Tianhua Xu, Ziyihui Wang, Yize Liu, Huaixu Chen, Junfeng Jiang, and Tiegen Liu
Biomed. Opt. Express 14(7) 3763-3774 (2023)

Dual-optofluidic waveguide in-line fiber biosensor for real-time label-free detection of interferon-gamma with temperature compensation

Ran Gao, Danfeng Lu, Dong Guo, and Xiangjun Xin
Opt. Express 28(7) 10491-10504 (2020)

Humanoid shaped optical fiber plasmon biosensor functionalized with graphene oxide/multi-walled carbon nanotubes for histamine detection

Wen Zhang, Ragini Singh, Zhi Wang, Guoru Li, Yiyan Xie, Rajan Jha, Carlos Marques, Bingyuan Zhang, and Santosh Kumar
Opt. Express 31(7) 11788-11803 (2023)

Functionalized optical fiber ball-shaped biosensor for label-free, low-limit detection of IL-8 protein

Gyunel Rashidova, Meruyert Tilegen, Tri T. Pham, Aliya Bekmurzayeva, and Daniele Tosi
Biomed. Opt. Express 15(1) 185-198 (2024)