Abstract

Clinical microscopy is a versatile and robust tool used for the diagnosis of a plethora of diseases. However, due to various reasons, it remains inaccessible in resource limited settings. In this paper, we present an automated and cost-effective alternative to microscopy for use in clinical diagnostics. With the use of custom optics and microfluidics, we demonstrate a field-portable imaging flow cytometry system. Using the presented system, we have been able to image 586 cells per second. We demonstrate the clinical relevance of the proposed system by differentiating between suspensions of healthy and sphered RBCs based on high-throughput morphometric analysis. The instrument presented here is a major advancement in the domain of field portable diagnostics as it enables fast and robust quantitative diagnostic testing at the point-of-care.

© 2015 IEEE

Cytopathological image analysis using deep-learning networks in microfluidic microscopy

G. Gopakumar, K. Hari Babu, Deepak Mishra, Sai Siva Gorthi, and Gorthi. R. K. Sai Subrahmanyam
J. Opt. Soc. Am. A 34(1) 111-121 (2017)

Label-free imaging flow cytometer for analyzing large cell populations by line-field quantitative phase microscopy with digital refocusing

Hidenao Yamada, Amane Hirotsu, Daisuke Yamashita, Osamu Yasuhiko, Toyohiko Yamauchi, Tsukasa Kayou, Hiroaki Suzuki, Shigetoshi Okazaki, Hirotoshi Kikuchi, Hiroya Takeuchi, and Yukio Ueda
Biomed. Opt. Express 11(4) 2213-2223 (2020)

In vivo imaging flow cytometer

Ho Lee, Clemens Alt, Costas M. Pitsillides, Mehron Puoris’haag, and Charles P. Lin
Opt. Express 14(17) 7789-7800 (2006)

Portable optofluidic absorption flow analyzer for quantitative malaria diagnosis from whole blood

Earu Banoth, Vamshi Krishna Kasula, and Sai Siva Gorthi
Appl. Opt. 55(30) 8637-8643 (2016)

Bead-based multiplex detection of dengue biomarkers in a portable imaging device

Xilong Yuan, Srishti Garg, Kevin De Haan, Frederic A. Fellouse, Anupriya Gopalsamy, Jan Tykvart, Sachdev S. Sidhu, Manoj M. Varma, Parama Pal, Edith M. Hillan, James Jiahua Dou, and J. Stewart Aitchison
Biomed. Opt. Express 11(11) 6154-6167 (2020)