Abstract

This paper presents a new label-free DNA optical sensor based on the microgap structured Fabry–Perot interferometer. An optical cavity is formed by a microgap sealed between a singlemode fiber and a well-cleaved fiber end. The capture DNA is immobilized on the cavity end by layer-by-layer electrostatic self-assembly. This sensor detects the hybridization of target DNA with complementary capture DNA by observing the optical cavity thickness variation. Experimental results demonstrate that this sensor has a sensitivity of 6 nmol/ml and can identify a 2-bp mismatch.

© 2008 IEEE

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  1. P. A. E. Piunno, U. J. Krull, R. H. E. Hudson, M. J. Damha, H. Cohen, "Fiber optic biosensor for fluorimetric detection of DNA hybridization," Anal. Chim. Acta 288, 205-214 (1994).
  2. K. Elena, A. Matt, B. Anastasia, "Direct electrochemical sensor for fast reagent-free DNA detection," Biosens. Bioelectron. 21, 182-189 (2005).
  3. Z. Sun, Y. He, J. Guo, "Surface plasmon resonance sensor based on polarization interferometry and angle modulation," Appl. Opt. 45, 3071-3076 (2006).
  4. H. Shibru, Y. Zhang, K. L. Cooper, "Optimization of layer-by-layer electrostatic self-assembly processing parameters for optical biosensing," Opt. Eng. 45, 024401 (2006).
  5. Y. Lvov, H. Haas, G. Decher, H. Mohwald, "Successive deposition of alternate layers of polyelectrolytes and a charged virus," Langmuir 10, 4232-4236 (1994).
  6. R. K. Iler, The Chemistry of Silica: Solubility, Polymerization, Colloid and Surface Properties, and Biochemistry (Wiley, 1997) pp. 467.
  7. D. E. Gray, S. C. Case-Green, T. S. Fell, P. J. Dobson, E. M. Southern, "Ellipsometric and interferometric characterization of DNA probes immobilized on a combinatorial array," Langmuir 13, 2833-2842 (1997).
  8. S. Elhadj, G. Singh, R. F. Saraf, "Optical properties of an immobilized DNA monolayer from 255 to 700 nm," Langmuir 20, 5539-5543 (2004).
  9. X. Wang, K. L. Cooper, A. Wang, J. Xu, Z. Wang, Y. Zhang, Z. Tu, "Label-free DNA sequence detection using oligonucleotide functionalized optical fiber," Appl. Phys. Lett. 89, 163901 (2006).
  10. X. Shi, R. J. Sanedrin, F. Zhou, "Structural character multilayered DNA and polylysine composite films: Influence of ionicof DNA solution on the extension of DNAincorporation," Phys. Chem. B 106, 1173-1180 (2001).
  11. Y. Zhang, X. Chen, Y. Wang, Y. Wang, K. L. Cooper, A. Wang, "Microgap multicavity Fabry–Perot biosensor," J. Lightw. Technol. 25, 1797-1804 (2007).
  12. C. Riccardi, H. Yamanaka, M. Josowicz, J. Kowalik, B. Mizaikoff, C. Kranz, "Label-Free DNA detection based on modified conducting Polypyrrole films at microelectrodes," Anal. Chem. 78, 1139-1145 (2006).
  13. Z. Wang, J. R. Heflin, R. H. Stolen, S. Ramachandran, "Highly sensitive optical response of optical fiber long period gratings to nanometer-thick ionic self-assmebled multilayers," Appl. Phys. Lett. 86, 223104 (2005).
  14. H. Kurt, D. S. Citrin, "Coupled-resonator optical waveguides for biochemical sensing of nanoliter volumes of analyte in the terahertz region," Appl. Phys. Lett. 87, 241119 (2005).
  15. X. Wang, U. J. Krull, "A self-contained fluorescent fiber optic DNA biosensor," J. Mater. Chem. 15, 2801-2809 (2005).
  16. M. Cornelius, C. G. C. Worth, H.-C. Kliem, M. Wiessler, H. H. Schmeiser, "Detection and separation of nucleoside-5 $^{\prime}$-monophosphates of DNA by conjugation with the fluorescent dye BODIPY and capillary electrophoresis with laer-induced fluorescence detection," Electrophoresis 26, 2591-2598 (2005).
  17. M. I. Pividori, A. Lermo, S. Campoy, J. Barbe, S. Hernandez, S. Aleqret, "In situ DNA amplification with magnetic primers for the electrochemical detection of food pathogens," Biosens. Bioelectron. 22, 2010-2011 (2007).
  18. K. Wang, Q. Wang, X. Yang, "Enhanced surface plasmon resonance for detection of DNA hybridization based on layer-by-layer assembly films," Sens. Actuators B 123, 227-232 (2007).

2007 (3)

Y. Zhang, X. Chen, Y. Wang, Y. Wang, K. L. Cooper, A. Wang, "Microgap multicavity Fabry–Perot biosensor," J. Lightw. Technol. 25, 1797-1804 (2007).

M. I. Pividori, A. Lermo, S. Campoy, J. Barbe, S. Hernandez, S. Aleqret, "In situ DNA amplification with magnetic primers for the electrochemical detection of food pathogens," Biosens. Bioelectron. 22, 2010-2011 (2007).

K. Wang, Q. Wang, X. Yang, "Enhanced surface plasmon resonance for detection of DNA hybridization based on layer-by-layer assembly films," Sens. Actuators B 123, 227-232 (2007).

2006 (4)

C. Riccardi, H. Yamanaka, M. Josowicz, J. Kowalik, B. Mizaikoff, C. Kranz, "Label-Free DNA detection based on modified conducting Polypyrrole films at microelectrodes," Anal. Chem. 78, 1139-1145 (2006).

Z. Sun, Y. He, J. Guo, "Surface plasmon resonance sensor based on polarization interferometry and angle modulation," Appl. Opt. 45, 3071-3076 (2006).

H. Shibru, Y. Zhang, K. L. Cooper, "Optimization of layer-by-layer electrostatic self-assembly processing parameters for optical biosensing," Opt. Eng. 45, 024401 (2006).

X. Wang, K. L. Cooper, A. Wang, J. Xu, Z. Wang, Y. Zhang, Z. Tu, "Label-free DNA sequence detection using oligonucleotide functionalized optical fiber," Appl. Phys. Lett. 89, 163901 (2006).

2005 (5)

K. Elena, A. Matt, B. Anastasia, "Direct electrochemical sensor for fast reagent-free DNA detection," Biosens. Bioelectron. 21, 182-189 (2005).

Z. Wang, J. R. Heflin, R. H. Stolen, S. Ramachandran, "Highly sensitive optical response of optical fiber long period gratings to nanometer-thick ionic self-assmebled multilayers," Appl. Phys. Lett. 86, 223104 (2005).

H. Kurt, D. S. Citrin, "Coupled-resonator optical waveguides for biochemical sensing of nanoliter volumes of analyte in the terahertz region," Appl. Phys. Lett. 87, 241119 (2005).

X. Wang, U. J. Krull, "A self-contained fluorescent fiber optic DNA biosensor," J. Mater. Chem. 15, 2801-2809 (2005).

M. Cornelius, C. G. C. Worth, H.-C. Kliem, M. Wiessler, H. H. Schmeiser, "Detection and separation of nucleoside-5 $^{\prime}$-monophosphates of DNA by conjugation with the fluorescent dye BODIPY and capillary electrophoresis with laer-induced fluorescence detection," Electrophoresis 26, 2591-2598 (2005).

2004 (1)

S. Elhadj, G. Singh, R. F. Saraf, "Optical properties of an immobilized DNA monolayer from 255 to 700 nm," Langmuir 20, 5539-5543 (2004).

2001 (1)

X. Shi, R. J. Sanedrin, F. Zhou, "Structural character multilayered DNA and polylysine composite films: Influence of ionicof DNA solution on the extension of DNAincorporation," Phys. Chem. B 106, 1173-1180 (2001).

1997 (1)

D. E. Gray, S. C. Case-Green, T. S. Fell, P. J. Dobson, E. M. Southern, "Ellipsometric and interferometric characterization of DNA probes immobilized on a combinatorial array," Langmuir 13, 2833-2842 (1997).

1994 (2)

P. A. E. Piunno, U. J. Krull, R. H. E. Hudson, M. J. Damha, H. Cohen, "Fiber optic biosensor for fluorimetric detection of DNA hybridization," Anal. Chim. Acta 288, 205-214 (1994).

Y. Lvov, H. Haas, G. Decher, H. Mohwald, "Successive deposition of alternate layers of polyelectrolytes and a charged virus," Langmuir 10, 4232-4236 (1994).

Anal. Chem. (1)

C. Riccardi, H. Yamanaka, M. Josowicz, J. Kowalik, B. Mizaikoff, C. Kranz, "Label-Free DNA detection based on modified conducting Polypyrrole films at microelectrodes," Anal. Chem. 78, 1139-1145 (2006).

Anal. Chim. Acta (1)

P. A. E. Piunno, U. J. Krull, R. H. E. Hudson, M. J. Damha, H. Cohen, "Fiber optic biosensor for fluorimetric detection of DNA hybridization," Anal. Chim. Acta 288, 205-214 (1994).

Appl. Opt. (1)

Appl. Phys. Lett. (3)

Z. Wang, J. R. Heflin, R. H. Stolen, S. Ramachandran, "Highly sensitive optical response of optical fiber long period gratings to nanometer-thick ionic self-assmebled multilayers," Appl. Phys. Lett. 86, 223104 (2005).

H. Kurt, D. S. Citrin, "Coupled-resonator optical waveguides for biochemical sensing of nanoliter volumes of analyte in the terahertz region," Appl. Phys. Lett. 87, 241119 (2005).

X. Wang, K. L. Cooper, A. Wang, J. Xu, Z. Wang, Y. Zhang, Z. Tu, "Label-free DNA sequence detection using oligonucleotide functionalized optical fiber," Appl. Phys. Lett. 89, 163901 (2006).

Biosens. Bioelectron. (2)

K. Elena, A. Matt, B. Anastasia, "Direct electrochemical sensor for fast reagent-free DNA detection," Biosens. Bioelectron. 21, 182-189 (2005).

M. I. Pividori, A. Lermo, S. Campoy, J. Barbe, S. Hernandez, S. Aleqret, "In situ DNA amplification with magnetic primers for the electrochemical detection of food pathogens," Biosens. Bioelectron. 22, 2010-2011 (2007).

Electrophoresis (1)

M. Cornelius, C. G. C. Worth, H.-C. Kliem, M. Wiessler, H. H. Schmeiser, "Detection and separation of nucleoside-5 $^{\prime}$-monophosphates of DNA by conjugation with the fluorescent dye BODIPY and capillary electrophoresis with laer-induced fluorescence detection," Electrophoresis 26, 2591-2598 (2005).

J. Lightw. Technol. (1)

Y. Zhang, X. Chen, Y. Wang, Y. Wang, K. L. Cooper, A. Wang, "Microgap multicavity Fabry–Perot biosensor," J. Lightw. Technol. 25, 1797-1804 (2007).

J. Mater. Chem. (1)

X. Wang, U. J. Krull, "A self-contained fluorescent fiber optic DNA biosensor," J. Mater. Chem. 15, 2801-2809 (2005).

Langmuir (3)

Y. Lvov, H. Haas, G. Decher, H. Mohwald, "Successive deposition of alternate layers of polyelectrolytes and a charged virus," Langmuir 10, 4232-4236 (1994).

D. E. Gray, S. C. Case-Green, T. S. Fell, P. J. Dobson, E. M. Southern, "Ellipsometric and interferometric characterization of DNA probes immobilized on a combinatorial array," Langmuir 13, 2833-2842 (1997).

S. Elhadj, G. Singh, R. F. Saraf, "Optical properties of an immobilized DNA monolayer from 255 to 700 nm," Langmuir 20, 5539-5543 (2004).

Opt. Eng. (1)

H. Shibru, Y. Zhang, K. L. Cooper, "Optimization of layer-by-layer electrostatic self-assembly processing parameters for optical biosensing," Opt. Eng. 45, 024401 (2006).

Phys. Chem. B (1)

X. Shi, R. J. Sanedrin, F. Zhou, "Structural character multilayered DNA and polylysine composite films: Influence of ionicof DNA solution on the extension of DNAincorporation," Phys. Chem. B 106, 1173-1180 (2001).

Sens. Actuators B (1)

K. Wang, Q. Wang, X. Yang, "Enhanced surface plasmon resonance for detection of DNA hybridization based on layer-by-layer assembly films," Sens. Actuators B 123, 227-232 (2007).

Other (1)

R. K. Iler, The Chemistry of Silica: Solubility, Polymerization, Colloid and Surface Properties, and Biochemistry (Wiley, 1997) pp. 467.

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