Abstract
A miniaturized fluorescence detection system based on total internal reflection (TIR)
configuration, which is applicable to detecting the presence of biological materials labeled with
fluorescence dye in micro total analysis systems (<i>μ</i>TAS), is proposed.
In conventional fluorescence testing and analysis devices, interference between the excitation light
beam and the emitted light from dyes is unavoidable. This paper presents a fluorescence detection
system based on TIR configuration that allows the excitation light beam and the emitted light to be
spatially perpendicular to each other so as to minimize the interference where fluorescence emission
is detected at the orthogonal angle to the excitation beam. We achieved the limit of detection of
about 5 nmol/L with a high linearity of 0.994 over a wide range of 6-FAM mol concentration, being
comparable to that in earlier studies.
© 2013 Optical Society of Korea
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