Abstract

Larynx cancer is a potentially terminal and severe type of neck and head cancer in which malignant cells start to grow and spread upwards in the larynx, or voice box. Smoking tobacco, drinking hot beverages and drinking alcohol are the main risk factors for these tumors. In this study, we aimed to develop a precise, accurate and rapid chemometrics assisted Raman spectroscopy method for diagnosis of larynx cancer in deparaffinized tissue samples. In the proposed method, samples were deparaffinized and 20 microns of each tissue were located on a coverslip. Both healthy (n = 13) and cancerous tissues (n = 13) were exposed to a Raman laser (785 nm) and excitations were recorded between wavenumbers of 50~1500 cm-1. An Orthogonal Partial Least Square algorithm was applied to evaluate the Raman spectrum obtained. Sensitivity and specificity of the proposed method is high enough with the aid of Principal Component Analysis (PCA) to test the whole model. Healthy and cancerous tissues were accurately and precisely clustered. A rapid, easy and precise diagnosis algorithm was developed for larynx cancer. By this method, some useful data about differences in biomolecules of each group (phospholipids, amides, tyrosine, phenylalanine collagen etc.) was also obtained from the spectra. It is claimed that the optimized method has a great potential for clustering and separating tumor tissues from healthy ones. This novel, rapid, precise and objective diagnosis method may be an alternative for the conventional methods in literature for diagnosis of larynx cancer.

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  14. R. G. Snyder, D. G. Cameron, H. L. Casal, D. A. C. Compton, and H. H. MantschStudies on determining conformational order in n-alkanes and phospholipids from the 1130 cm-1 raman bandBiochim. Biophys. Acta, Biomembr.1982684111116
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Other (15)

J. F. Petersen, M. M. Stuiver, A. J. Timmermans, A. Chen, H. Z. Zhang, J. P. O’Neill, S. Deady, V. Vander Poorten, J. Meulemans, J. Wennerberg, C. Skroder, A. T. Day, W. Koch, and M. W. M. van den BrekelDevelopment and external validation of a risk-prediction model to predict 5-year overall survival in advanced larynx cancerLaryngoscope201812811401145

C. Salvador-Coloma and E. CohenMultidisciplinary care of laryngeal cancerJ. Oncol. Pharm. Pract.201612717724

H. T. Hoffman, K. Porter, L. H. Karnell, J. S. Cooper, R. S. Weber, C. J. Langer, K. K. Ang, G. Gay, A. Stewart, and R. A. RobinsonLaryngeal cancer in the United States: changes in demographics, patterns of care, and survivalLaryngoscope2006116113

E. De Stefani, P. Correa, F. Oreggia, J. Leiva, S. Rivero, G. Fernandez, H. Deneo-Pellegrini, D. Zavala, and E. FonthamRisk factors for laryngeal cancerCancer19876030873091

A. J. Tuyns, J. Esteve, L. Raymond, F. Berrino, E. Benhamou, F. Blanchet, P. Boffetta, P. Crosignani, A. D. Moral, and W. LehmannCancer of the larynx/hypopharynx, tobacco and alcohol: Iarc international case-control study in Turin and Varese (Italy), Zaragoza and Navarra (Spain), Geneva (Switzerland) and Calvados (France)Int. J. Cancer198841483491

M. H. McGavran, W. C. Bauer, and J. H. OguraThe incidence of cervical lymph node metastases from epidermoid carcinoma of the larynx and their relationship to certain characteristics of the primary tumor. A study based on the clinical and pathological findings for 96 patients treated by primary en bloc laryngectomy and radical neck dissectionCancer1961145566

M. E. Haisfield-Wolfe, C. Brown, M. Richardson, and K. WebsterVariations in symptom severity patterns among oropharyngeal and laryngeal cancer outpatients during radiation treatment: A pilot studyCancer Nurs.201538279287

D. K. Sharma, B. S. Sohal, M. S. Bal, and S. AggarwalClinico-pathological study of 50 cases of tumours of larynxIndian J. Otolaryngol. Head Neck Surg.2013652935

L. Licitra, J. Bernier, C. Grandi, L. Locati, M. Merlano, G. Gatta, and J.-L. LefebvreCancer of the larynxCrit. Rev. Oncol. Hematol.2003476580

R. L. Siegel, K. D. Miller, and A. JemalCancer statistics, 2018CA Cancer J. Clin.201868730

V. L. Shavers, L. C. Harlan, D. Winn, and W. W. DavisRacial/ethnic patterns of care for cancers of the oral cavity, pharynx, larynx, sinuses, and salivary glandsCancer Metastasis Rev.2003222538

S. Cheraghlou, P. Kuo, S. Mehra, W. G. Yarbrough, and B. L. JudsonSalvage surgery after radiation failure in T1/T2 larynx cancer: Outcomes following total versus conservation surgeryOtolaryngol. Head Neck Surg.2018158497504

V. Bajaj, Y. Guo, A. Sengur, S. Siuly, and O. F. AlcinA hybrid method based on time-frequency images for classification of alcohol and control EEG signalsNeural Computing and Applications20172837173723

R. G. Snyder, D. G. Cameron, H. L. Casal, D. A. C. Compton, and H. H. MantschStudies on determining conformational order in n-alkanes and phospholipids from the 1130 cm-1 raman bandBiochim. Biophys. Acta, Biomembr.1982684111116

C. Mazouni, B. Arun, F. André, M. Ayers, S. Krishnamurthy, B. Wang, G. N. Hortobagyi, A. U. Buzdar, and L. PusztaiCollagen IV levels are elevated in the serum of patients with primary breast cancer compared to healthy volunteersBr. J. Cancer2008996871

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