Abstract
The morphological changes of anthracnose (fungus) -infected tomato
seeds have been studied to identify the infection and characterize its
effect. Full-field optical coherence tomography (FF-OCT) has been utilized
as a nondestructive but efficient modality for visualizing the effects of
fungal infection. The cross-sectional images extracted from a stack of en
face FF-OCT images showed significant changes with infection in the seed
structure. First of all, the seed coat disappeared with the infection. The
thickness of the seed coat of a healthy seed was measured as 28.2 µm, with
a standard deviation of 1.2 µm. However, for infected seeds the gap
between surface and endosperm was not appreciably observed. In addition,
the measurements confirmed that the dryness of seeds did not affect the
internal seed structure. The reconstructed three-dimensional (3D) image
revealed that the permeability of the seed coat, which plays the vital
role of protecting the seed, is also affected by the infection. These
results suggest that FF-OCT has good potential for the identification of
fungus-infected tomato seeds, and for many other tasks in
agriculture.
© 2019 Optical Society of Korea
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