Abstract

A reflection-mode photoacoustic microscope (PAM) for rat brain imaging in vivo is constructed. A pulsed laser is used as an excitation source, and a focused ultrasound transducer is adopted to collect the photoacoustic signal. Raster scanning is applied to acquire three-dimensional (3D) data. The obtained measurements of the lateral and axial resolutions of the microscope are 45 and 15 \mu m, respectively. The imaging depth in the chicken breast tissue is 3.1 mm at a signal-to-noise ratio (SNR) of 20 dB without any signal averaging. The imaging speed is 30 A-line/s. Experimental results in vivo demonstrate the capability of 3D imaging of the brain vessels of the rat after removing the skull.

© 2010 Chinese Optics Letters

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2009 (5)

C. Li and L. V. Wang, Phys. Med. Biol. 54, R59 (2009).

L. V. Wang, Nature Photon. 3, 503 (2009).

E. W. Stein, K. Maslov, and L. V. Wang, J. Appl. Phys. 105, 102027 (2009).

E. W. Stein, K. Maslov, and L. V. Wang, J. Biomed. Opt. 14, 020502 (2009).

H. Guo and S. Yang, Rev. Sci. Instrum. 80, 014903 (2009).

2008 (2)

C. Zhang and Y. Wang, Acta Opt. Sin. (in Chinese) 28, 2296 (2008).

Z. Wang and Q. Liu, Prog. Biochem. Biophys. (in Chinese) 35, 488 (2008).

2007 (2)

H. Zhang, Z. Tang, Y. He, and L. Guo, Rev. Sci. Instrum. 78, 064902 (2007).

L. Xiang, D. Xing, H. Gu, D. Yang, S. Yang, L. Zeng, and W. Chen, J. Biomed. Opt. 12, 014001 (2007).

2006 (3)

M. Xu and L. V. Wang, Rev. Sci. Instrum. 77, 041101 (2006).

D. Yang, D. Xing, Y. Tan, H. Gu, and S. Yang, Appl. Phys. Lett. 88, 174101 (2006).

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nature Biotechnol. 24, 848 (2006).

2005 (1)

F. Helmchen and W. Denk, Nature Methods 2, 932 (2005).

2002 (1)

M. D. Cahalan, I. Parker, S. H. Wei, and M. J. Miller, Nat. Rev. Immunol. 2, 872 (2002).

2000 (1)

R. Cabeza and L. Nyberg, J. Cognit. Neurosci. 12, 1 (2000).

1999 (1)

C. Guittet, F. Ossant, L. Vaillant, and M. Berson, IEEE Trans. Biomed. Eng. 46, 740 (1999).

1991 (1)

G. J. Diebold, T. Sun, and M. I. Khan, Phys. Rev. Lett. 67, 3384 (1991).

Berson, M.

C. Guittet, F. Ossant, L. Vaillant, and M. Berson, IEEE Trans. Biomed. Eng. 46, 740 (1999).

Cabeza, R.

R. Cabeza and L. Nyberg, J. Cognit. Neurosci. 12, 1 (2000).

Cahalan, M. D.

M. D. Cahalan, I. Parker, S. H. Wei, and M. J. Miller, Nat. Rev. Immunol. 2, 872 (2002).

Chen, W.

L. Xiang, D. Xing, H. Gu, D. Yang, S. Yang, L. Zeng, and W. Chen, J. Biomed. Opt. 12, 014001 (2007).

Denk, W.

F. Helmchen and W. Denk, Nature Methods 2, 932 (2005).

Diebold, G. J.

G. J. Diebold, T. Sun, and M. I. Khan, Phys. Rev. Lett. 67, 3384 (1991).

Gu, H.

L. Xiang, D. Xing, H. Gu, D. Yang, S. Yang, L. Zeng, and W. Chen, J. Biomed. Opt. 12, 014001 (2007).

D. Yang, D. Xing, Y. Tan, H. Gu, and S. Yang, Appl. Phys. Lett. 88, 174101 (2006).

Guittet, C.

C. Guittet, F. Ossant, L. Vaillant, and M. Berson, IEEE Trans. Biomed. Eng. 46, 740 (1999).

Guo, H.

H. Guo and S. Yang, Rev. Sci. Instrum. 80, 014903 (2009).

Guo, L.

H. Zhang, Z. Tang, Y. He, and L. Guo, Rev. Sci. Instrum. 78, 064902 (2007).

He, Y.

H. Zhang, Z. Tang, Y. He, and L. Guo, Rev. Sci. Instrum. 78, 064902 (2007).

Helmchen, F.

F. Helmchen and W. Denk, Nature Methods 2, 932 (2005).

Khan, M. I.

G. J. Diebold, T. Sun, and M. I. Khan, Phys. Rev. Lett. 67, 3384 (1991).

Li, C.

C. Li and L. V. Wang, Phys. Med. Biol. 54, R59 (2009).

Liu, Q.

Z. Wang and Q. Liu, Prog. Biochem. Biophys. (in Chinese) 35, 488 (2008).

Maslov, K.

E. W. Stein, K. Maslov, and L. V. Wang, J. Appl. Phys. 105, 102027 (2009).

E. W. Stein, K. Maslov, and L. V. Wang, J. Biomed. Opt. 14, 020502 (2009).

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nature Biotechnol. 24, 848 (2006).

Miller, M. J.

M. D. Cahalan, I. Parker, S. H. Wei, and M. J. Miller, Nat. Rev. Immunol. 2, 872 (2002).

Nyberg, L.

R. Cabeza and L. Nyberg, J. Cognit. Neurosci. 12, 1 (2000).

Ossant, F.

C. Guittet, F. Ossant, L. Vaillant, and M. Berson, IEEE Trans. Biomed. Eng. 46, 740 (1999).

Parker, I.

M. D. Cahalan, I. Parker, S. H. Wei, and M. J. Miller, Nat. Rev. Immunol. 2, 872 (2002).

Stein, E. W.

E. W. Stein, K. Maslov, and L. V. Wang, J. Biomed. Opt. 14, 020502 (2009).

E. W. Stein, K. Maslov, and L. V. Wang, J. Appl. Phys. 105, 102027 (2009).

Stoica, G.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nature Biotechnol. 24, 848 (2006).

Sun, T.

G. J. Diebold, T. Sun, and M. I. Khan, Phys. Rev. Lett. 67, 3384 (1991).

Tan, Y.

D. Yang, D. Xing, Y. Tan, H. Gu, and S. Yang, Appl. Phys. Lett. 88, 174101 (2006).

Tang, Z.

H. Zhang, Z. Tang, Y. He, and L. Guo, Rev. Sci. Instrum. 78, 064902 (2007).

Vaillant, L.

C. Guittet, F. Ossant, L. Vaillant, and M. Berson, IEEE Trans. Biomed. Eng. 46, 740 (1999).

Wang, L. V.

E. W. Stein, K. Maslov, and L. V. Wang, J. Biomed. Opt. 14, 020502 (2009).

E. W. Stein, K. Maslov, and L. V. Wang, J. Appl. Phys. 105, 102027 (2009).

C. Li and L. V. Wang, Phys. Med. Biol. 54, R59 (2009).

L. V. Wang, Nature Photon. 3, 503 (2009).

M. Xu and L. V. Wang, Rev. Sci. Instrum. 77, 041101 (2006).

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nature Biotechnol. 24, 848 (2006).

Wang, Y.

C. Zhang and Y. Wang, Acta Opt. Sin. (in Chinese) 28, 2296 (2008).

Wang, Z.

Z. Wang and Q. Liu, Prog. Biochem. Biophys. (in Chinese) 35, 488 (2008).

Wei, S. H.

M. D. Cahalan, I. Parker, S. H. Wei, and M. J. Miller, Nat. Rev. Immunol. 2, 872 (2002).

Xiang, L.

L. Xiang, D. Xing, H. Gu, D. Yang, S. Yang, L. Zeng, and W. Chen, J. Biomed. Opt. 12, 014001 (2007).

Xing, D.

L. Xiang, D. Xing, H. Gu, D. Yang, S. Yang, L. Zeng, and W. Chen, J. Biomed. Opt. 12, 014001 (2007).

D. Yang, D. Xing, Y. Tan, H. Gu, and S. Yang, Appl. Phys. Lett. 88, 174101 (2006).

Xu, M.

M. Xu and L. V. Wang, Rev. Sci. Instrum. 77, 041101 (2006).

Yang, D.

L. Xiang, D. Xing, H. Gu, D. Yang, S. Yang, L. Zeng, and W. Chen, J. Biomed. Opt. 12, 014001 (2007).

D. Yang, D. Xing, Y. Tan, H. Gu, and S. Yang, Appl. Phys. Lett. 88, 174101 (2006).

Yang, S.

H. Guo and S. Yang, Rev. Sci. Instrum. 80, 014903 (2009).

L. Xiang, D. Xing, H. Gu, D. Yang, S. Yang, L. Zeng, and W. Chen, J. Biomed. Opt. 12, 014001 (2007).

D. Yang, D. Xing, Y. Tan, H. Gu, and S. Yang, Appl. Phys. Lett. 88, 174101 (2006).

Zeng, L.

L. Xiang, D. Xing, H. Gu, D. Yang, S. Yang, L. Zeng, and W. Chen, J. Biomed. Opt. 12, 014001 (2007).

Zhang, C.

C. Zhang and Y. Wang, Acta Opt. Sin. (in Chinese) 28, 2296 (2008).

Zhang, H.

H. Zhang, Z. Tang, Y. He, and L. Guo, Rev. Sci. Instrum. 78, 064902 (2007).

Zhang, H. F.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nature Biotechnol. 24, 848 (2006).

Acta Opt. Sin. (in Chinese) (1)

C. Zhang and Y. Wang, Acta Opt. Sin. (in Chinese) 28, 2296 (2008).

Appl. Phys. Lett. (1)

D. Yang, D. Xing, Y. Tan, H. Gu, and S. Yang, Appl. Phys. Lett. 88, 174101 (2006).

IEEE Trans. Biomed. Eng. (1)

C. Guittet, F. Ossant, L. Vaillant, and M. Berson, IEEE Trans. Biomed. Eng. 46, 740 (1999).

J. Appl. Phys. (1)

E. W. Stein, K. Maslov, and L. V. Wang, J. Appl. Phys. 105, 102027 (2009).

J. Biomed. Opt. (2)

E. W. Stein, K. Maslov, and L. V. Wang, J. Biomed. Opt. 14, 020502 (2009).

L. Xiang, D. Xing, H. Gu, D. Yang, S. Yang, L. Zeng, and W. Chen, J. Biomed. Opt. 12, 014001 (2007).

J. Cognit. Neurosci. (1)

R. Cabeza and L. Nyberg, J. Cognit. Neurosci. 12, 1 (2000).

Nat. Rev. Immunol. (1)

M. D. Cahalan, I. Parker, S. H. Wei, and M. J. Miller, Nat. Rev. Immunol. 2, 872 (2002).

Nature Biotechnol. (1)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nature Biotechnol. 24, 848 (2006).

Nature Methods (1)

F. Helmchen and W. Denk, Nature Methods 2, 932 (2005).

Nature Photon. (1)

L. V. Wang, Nature Photon. 3, 503 (2009).

Phys. Med. Biol. (1)

C. Li and L. V. Wang, Phys. Med. Biol. 54, R59 (2009).

Phys. Rev. Lett. (1)

G. J. Diebold, T. Sun, and M. I. Khan, Phys. Rev. Lett. 67, 3384 (1991).

Prog. Biochem. Biophys. (in Chinese) (1)

Z. Wang and Q. Liu, Prog. Biochem. Biophys. (in Chinese) 35, 488 (2008).

Rev. Sci. Instrum. (3)

M. Xu and L. V. Wang, Rev. Sci. Instrum. 77, 041101 (2006).

H. Zhang, Z. Tang, Y. He, and L. Guo, Rev. Sci. Instrum. 78, 064902 (2007).

H. Guo and S. Yang, Rev. Sci. Instrum. 80, 014903 (2009).

Other (1)

C. Passmann and H. Ermert, in Proceedings of IEEE Ultrasonics Symposium 1661 (1994).

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