Abstract

Streak camera has high temporal resolution and high sensitivity, and is a powerful tool in biomedical study to measure fluorescence lifetime and perform fluorescence lifetime imaging. However, nonuniformity of the gain in the streak tube and nonlinearity of the sweeping speed limit the precision of fluorescence lifetime measurement, particularly when fluorescence lifetimes are short. We have constructed a twophoton excitation fluorescence lifetime measurement system that is based on a synchroscan streak camera and have developed accordingly a method to correct the effect of gain nonuniformity and nonlinearity of sweeping speed on the measurement precision. A continuous-wave laser of high stability is used to calibrate the gain of the streak camera, and a Fabry-Perot etalon is used to calibrate the nonlinearity of the sweeping speed. Fitting algorithms are used to correct the gain of the streak camera and nonlinearity of the sweeping speed respectively, which significantly improves the measurement precision of the system, as characterized through the fluorescence lifetime of the short-lived fluorescence dye, Rose Bengal. Experimental results show that the measurement fluctuation of the lifetime has been improved from more than 10% to 2% after correcting the effects of gain nonuniformity and sweeping speed nonlinearity.

© 2010 Chinese Optics Letters

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  18. http://www.iss.com/resources/lifetime.html.(September 11, 2010)

2010 (1)

H. Li, Y. Shao, Y. Wang, J. Qu, Y. An, and H. Niu, Chinese J. Lasers (in Chinese) 37, 1240 (2010).

2009 (1)

Y. Shao, H. Li, Y. Wang, J. Qu, and H. Niu, Journal of Shenzhen University Science and Engineering (in Chinese) 26, 349 (2009).

2006 (1)

L. Liu, J. Qu, Z. Lin, D. Chen, B. Guo, and H. Niu, Acta Opt. Sin. (in Chinese) 26, 373 (2006).

2005 (1)

H. Wallrabe and A. Periasamy, Current Opinion in Biotechnol. 16, 19 (2005).

2004 (2)

W. Becker, A. Bergmann, M. A. Hink, K. K¨onig, K. Benndorf, and C. Biskup, Microsc. Res. Tech. 63, 58 (2004).

D. K. Bird, K. W. Eliceiri, C.-H. Fan, and J. G. White, Appl. Opt. 43, 5173 (2004).

2003 (2)

H.-J. Lin, P. Herman, and J. R. Lakowicz, Cytometry Part A 52A, 77 (2003).

R. V. Krishnan, A. Masuda, V. E. Centonze, and B. Herman, J. Biomed. Opt. 8, 362 (2003).

2002 (1)

1999 (2)

R. Pepperkok, A. Squire, S. Geley, and P. I. H. Bastiaens, Current Biology 9, 269 (1999).

S. Weiss, Science 283, 1676 (1999).

1997 (3)

B. R. Masters, P. T. C. So, and E. Gratton, Biophys. J. 76, 2405 (1997).

H. Brismar and B. Ulfhake, Nature Biotechnol. 15, 373(1997).

K. Dowling, S. C. W. Hyde, J. C. Dainty, P. M. W. French, and J. D. Hares, Opt. Commun. 135, 27 (1997).

1969 (1)

R. D. Spencer and G. Weber, Ann. NY Acad. Sci. 158, 361 (1969).

1927 (1)

V. E. Gaviola, Zeitschrift f¨ur Physik A Hadrons and Nuclei (in German) 42, 853 (1927).

An, Y.

H. Li, Y. Shao, Y. Wang, J. Qu, Y. An, and H. Niu, Chinese J. Lasers (in Chinese) 37, 1240 (2010).

Arndt-Jovin, D. J.

Bastiaens, P. I. H.

R. Pepperkok, A. Squire, S. Geley, and P. I. H. Bastiaens, Current Biology 9, 269 (1999).

Becker, W.

W. Becker, A. Bergmann, M. A. Hink, K. K¨onig, K. Benndorf, and C. Biskup, Microsc. Res. Tech. 63, 58 (2004).

Benndorf, K.

W. Becker, A. Bergmann, M. A. Hink, K. K¨onig, K. Benndorf, and C. Biskup, Microsc. Res. Tech. 63, 58 (2004).

Bergmann, A.

W. Becker, A. Bergmann, M. A. Hink, K. K¨onig, K. Benndorf, and C. Biskup, Microsc. Res. Tech. 63, 58 (2004).

Bird, D. K.

Biskup, C.

W. Becker, A. Bergmann, M. A. Hink, K. K¨onig, K. Benndorf, and C. Biskup, Microsc. Res. Tech. 63, 58 (2004).

Brismar, H.

H. Brismar and B. Ulfhake, Nature Biotechnol. 15, 373(1997).

Centonze, V. E.

R. V. Krishnan, A. Masuda, V. E. Centonze, and B. Herman, J. Biomed. Opt. 8, 362 (2003).

Chen, D.

L. Liu, J. Qu, Z. Lin, D. Chen, B. Guo, and H. Niu, Acta Opt. Sin. (in Chinese) 26, 373 (2006).

Dainty, J. C.

K. Dowling, S. C. W. Hyde, J. C. Dainty, P. M. W. French, and J. D. Hares, Opt. Commun. 135, 27 (1997).

Dowling, K.

K. Dowling, S. C. W. Hyde, J. C. Dainty, P. M. W. French, and J. D. Hares, Opt. Commun. 135, 27 (1997).

Eliceiri, K. W.

Fan, C.-H.

French, P. M. W.

K. Dowling, S. C. W. Hyde, J. C. Dainty, P. M. W. French, and J. D. Hares, Opt. Commun. 135, 27 (1997).

Gaviola, V. E.

V. E. Gaviola, Zeitschrift f¨ur Physik A Hadrons and Nuclei (in German) 42, 853 (1927).

Geley, S.

R. Pepperkok, A. Squire, S. Geley, and P. I. H. Bastiaens, Current Biology 9, 269 (1999).

Gratton, E.

B. R. Masters, P. T. C. So, and E. Gratton, Biophys. J. 76, 2405 (1997).

Guo, B.

L. Liu, J. Qu, Z. Lin, D. Chen, B. Guo, and H. Niu, Acta Opt. Sin. (in Chinese) 26, 373 (2006).

Hanley, Q. S.

Hares, J. D.

K. Dowling, S. C. W. Hyde, J. C. Dainty, P. M. W. French, and J. D. Hares, Opt. Commun. 135, 27 (1997).

Herman, B.

R. V. Krishnan, A. Masuda, V. E. Centonze, and B. Herman, J. Biomed. Opt. 8, 362 (2003).

Herman, P.

H.-J. Lin, P. Herman, and J. R. Lakowicz, Cytometry Part A 52A, 77 (2003).

Hink, M. A.

W. Becker, A. Bergmann, M. A. Hink, K. K¨onig, K. Benndorf, and C. Biskup, Microsc. Res. Tech. 63, 58 (2004).

Hyde, S. C. W.

K. Dowling, S. C. W. Hyde, J. C. Dainty, P. M. W. French, and J. D. Hares, Opt. Commun. 135, 27 (1997).

Jovin, T. M.

K¨onig, K.

W. Becker, A. Bergmann, M. A. Hink, K. K¨onig, K. Benndorf, and C. Biskup, Microsc. Res. Tech. 63, 58 (2004).

Krishnan, R. V.

R. V. Krishnan, A. Masuda, V. E. Centonze, and B. Herman, J. Biomed. Opt. 8, 362 (2003).

Lakowicz, J. R.

H.-J. Lin, P. Herman, and J. R. Lakowicz, Cytometry Part A 52A, 77 (2003).

Li, H.

H. Li, Y. Shao, Y. Wang, J. Qu, Y. An, and H. Niu, Chinese J. Lasers (in Chinese) 37, 1240 (2010).

Y. Shao, H. Li, Y. Wang, J. Qu, and H. Niu, Journal of Shenzhen University Science and Engineering (in Chinese) 26, 349 (2009).

Lin, H.-J.

H.-J. Lin, P. Herman, and J. R. Lakowicz, Cytometry Part A 52A, 77 (2003).

Lin, Z.

L. Liu, J. Qu, Z. Lin, D. Chen, B. Guo, and H. Niu, Acta Opt. Sin. (in Chinese) 26, 373 (2006).

Liu, L.

L. Liu, J. Qu, Z. Lin, D. Chen, B. Guo, and H. Niu, Acta Opt. Sin. (in Chinese) 26, 373 (2006).

Masters, B. R.

B. R. Masters, P. T. C. So, and E. Gratton, Biophys. J. 76, 2405 (1997).

Masuda, A.

R. V. Krishnan, A. Masuda, V. E. Centonze, and B. Herman, J. Biomed. Opt. 8, 362 (2003).

Niu, H.

H. Li, Y. Shao, Y. Wang, J. Qu, Y. An, and H. Niu, Chinese J. Lasers (in Chinese) 37, 1240 (2010).

Y. Shao, H. Li, Y. Wang, J. Qu, and H. Niu, Journal of Shenzhen University Science and Engineering (in Chinese) 26, 349 (2009).

L. Liu, J. Qu, Z. Lin, D. Chen, B. Guo, and H. Niu, Acta Opt. Sin. (in Chinese) 26, 373 (2006).

Pepperkok, R.

R. Pepperkok, A. Squire, S. Geley, and P. I. H. Bastiaens, Current Biology 9, 269 (1999).

Periasamy, A.

H. Wallrabe and A. Periasamy, Current Opinion in Biotechnol. 16, 19 (2005).

Qu, J.

H. Li, Y. Shao, Y. Wang, J. Qu, Y. An, and H. Niu, Chinese J. Lasers (in Chinese) 37, 1240 (2010).

Y. Shao, H. Li, Y. Wang, J. Qu, and H. Niu, Journal of Shenzhen University Science and Engineering (in Chinese) 26, 349 (2009).

L. Liu, J. Qu, Z. Lin, D. Chen, B. Guo, and H. Niu, Acta Opt. Sin. (in Chinese) 26, 373 (2006).

Shao, Y.

H. Li, Y. Shao, Y. Wang, J. Qu, Y. An, and H. Niu, Chinese J. Lasers (in Chinese) 37, 1240 (2010).

Y. Shao, H. Li, Y. Wang, J. Qu, and H. Niu, Journal of Shenzhen University Science and Engineering (in Chinese) 26, 349 (2009).

So, P. T. C.

B. R. Masters, P. T. C. So, and E. Gratton, Biophys. J. 76, 2405 (1997).

Spencer, R. D.

R. D. Spencer and G. Weber, Ann. NY Acad. Sci. 158, 361 (1969).

Squire, A.

R. Pepperkok, A. Squire, S. Geley, and P. I. H. Bastiaens, Current Biology 9, 269 (1999).

Ulfhake, B.

H. Brismar and B. Ulfhake, Nature Biotechnol. 15, 373(1997).

Wallrabe, H.

H. Wallrabe and A. Periasamy, Current Opinion in Biotechnol. 16, 19 (2005).

Wang, Y.

H. Li, Y. Shao, Y. Wang, J. Qu, Y. An, and H. Niu, Chinese J. Lasers (in Chinese) 37, 1240 (2010).

Y. Shao, H. Li, Y. Wang, J. Qu, and H. Niu, Journal of Shenzhen University Science and Engineering (in Chinese) 26, 349 (2009).

Weber, G.

R. D. Spencer and G. Weber, Ann. NY Acad. Sci. 158, 361 (1969).

Weiss, S.

S. Weiss, Science 283, 1676 (1999).

White, J. G.

Acta Opt. Sin. (in Chinese) (1)

L. Liu, J. Qu, Z. Lin, D. Chen, B. Guo, and H. Niu, Acta Opt. Sin. (in Chinese) 26, 373 (2006).

Ann. NY Acad. Sci. (1)

R. D. Spencer and G. Weber, Ann. NY Acad. Sci. 158, 361 (1969).

Appl. Opt. (1)

Appl. Spectrosc. (1)

Biophys. J. (1)

B. R. Masters, P. T. C. So, and E. Gratton, Biophys. J. 76, 2405 (1997).

Chinese J. Lasers (in Chinese) (1)

H. Li, Y. Shao, Y. Wang, J. Qu, Y. An, and H. Niu, Chinese J. Lasers (in Chinese) 37, 1240 (2010).

Current Biology (1)

R. Pepperkok, A. Squire, S. Geley, and P. I. H. Bastiaens, Current Biology 9, 269 (1999).

Current Opinion in Biotechnol. (1)

H. Wallrabe and A. Periasamy, Current Opinion in Biotechnol. 16, 19 (2005).

Cytometry Part A (1)

H.-J. Lin, P. Herman, and J. R. Lakowicz, Cytometry Part A 52A, 77 (2003).

J. Biomed. Opt. (1)

R. V. Krishnan, A. Masuda, V. E. Centonze, and B. Herman, J. Biomed. Opt. 8, 362 (2003).

Journal of Shenzhen University Science and Engineering (in Chinese) (1)

Y. Shao, H. Li, Y. Wang, J. Qu, and H. Niu, Journal of Shenzhen University Science and Engineering (in Chinese) 26, 349 (2009).

Microsc. Res. Tech. (1)

W. Becker, A. Bergmann, M. A. Hink, K. K¨onig, K. Benndorf, and C. Biskup, Microsc. Res. Tech. 63, 58 (2004).

Nature Biotechnol. (1)

H. Brismar and B. Ulfhake, Nature Biotechnol. 15, 373(1997).

Opt. Commun. (1)

K. Dowling, S. C. W. Hyde, J. C. Dainty, P. M. W. French, and J. D. Hares, Opt. Commun. 135, 27 (1997).

Science (1)

S. Weiss, Science 283, 1676 (1999).

Zeitschrift f¨ur Physik A Hadrons and Nuclei (in German) (1)

V. E. Gaviola, Zeitschrift f¨ur Physik A Hadrons and Nuclei (in German) 42, 853 (1927).

Other (2)

http://www.iss.com/resources/lifetime.html.(September 11, 2010)

W. Denk, D. W. Piston, and W. W. Webb, in Handbook of Biological Confocal Microscopy J. B. Pawley, (ed.) (Plenum Press, New York, 1995)p. 445.

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