Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Chinese Optics Letters
  • Vol. 7,
  • Issue 10,
  • pp. 898-900
  • (2009)

Laser-based transfection with conjugated gold nanoparticles

Not Accessible

Your library or personal account may give you access

Abstract

The irradiation of cells combined with the immunoconjugate of gold nanoparticles by the short pulse laser can make the plasma membrane be transiently permeabilized, which can be used to transfer exogenous molecules into the cells. We explore this technique as a novel gene transfection method for floating cells. Three different floating cells exposed to the laser are selectively transfected with fluorescein isothiocyanatedextran, antibody, and green fluorescent protein (GFP) coding plasmids, and the viability of cells are determined by propidium iodide. For fluorescein isothiocyanate-dextran, the best transfection efficiency of 65% is obtained; for the antibody, it is 74%; whereas for the green fluorescent protein coding plasmids, a very small transfection efficiency is gained. If the transfection efficiency is improved, gold nanoparticles will be very useful as mediator for gene transfection in living cells.

© 2009 Chinese Optics Letters

PDF Article
More Like This
Laser-induced breakdown of an optically trapped gold nanoparticle for single cell transfection

Yoshihiko Arita, Martin Ploschner, Maciej Antkowiak, Frank Gunn-Moore, and Kishan Dholakia
Opt. Lett. 38(17) 3402-3405 (2013)

Single-cell optoporation and transfection using femtosecond laser and optical tweezers

Muhammad Waleed, Sun-Uk Hwang, Jung-Dae Kim, Irfan Shabbir, Sang-Mo Shin, and Yong-Gu Lee
Biomed. Opt. Express 4(9) 1533-1547 (2013)

Dielectric microsphere mediated transfection using a femtosecond laser

Mitsuhiro Terakawa and Yuto Tanaka
Opt. Lett. 36(15) 2877-2879 (2011)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved