Abstract

The irradiation of cells combined with the immunoconjugate of gold nanoparticles by the short pulse laser can make the plasma membrane be transiently permeabilized, which can be used to transfer exogenous molecules into the cells. We explore this technique as a novel gene transfection method for floating cells. Three different floating cells exposed to the laser are selectively transfected with fluorescein isothiocyanatedextran, antibody, and green fluorescent protein (GFP) coding plasmids, and the viability of cells are determined by propidium iodide. For fluorescein isothiocyanate-dextran, the best transfection efficiency of 65% is obtained; for the antibody, it is 74%; whereas for the green fluorescent protein coding plasmids, a very small transfection efficiency is gained. If the transfection efficiency is improved, gold nanoparticles will be very useful as mediator for gene transfection in living cells.

© 2009 Chinese Optics Letters

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2009

C. Yao and Z. Zhang, Acta Opt. Sin. (in Chinese) 29, 1610 (2009).

2008

X. Qu, J. Wang, C. Yao, and Z. Zhang, Chin. Opt. Lett. 6, 879 (2008)

C.-P. Yao, Z.-X. Zhang, R. Rahmanzadeh, and G. Huettmann, IEEE Trans. Nanobiosici. 7, 111 (2008).

D. Wang, J. Yu, C. Guo, and W. Qi, Chinese J. Lasers (in Chinese) 35, 1579 (2008).

Y. Wang, L. Wang, X. Deng, Y. Liu, and M. Bu, Chinese J. Lasers (in Chinese) 35, 1491 (2008).

2005

C. Yao, Z. Li, and Z. Zhang, Acta Opt. Sin. (in Chinese) 25, 1664 (2005).

C. Yao, R. Rahmanzadeh, E. Endl, Z. Zhang, J. Gerdes, and G. Huttmann, J. Biomed. Opt. 10, 064012 (2005).

A. Plech, V. Kotaidis, M. Lorenc, and M. Wulff, Chem. Phys. Lett. 401, 565 (2005).

2004

M. Terakawa, M. Ogura, S. Sato, H.Wakisaka, H. Ashida, M. Uenoyama, Y. Masaki, and M. Obara, Opt. Lett. 29, 1227 (2004).

A. Plech, V. Kotaidis, S. Gresillon, C. Dahmen, and G. von Plessen, Phys. Rev. B 70, 195423 (2004).

2003

C. M. Pitsillides, E. K. Joe, X. Wei, R. R. Anderson, and C. P. Lin, Biophysical Journal 84, 4023 (2003).

V. P. Zharov, V. Galitovsky, and M. Viegas, Appl. Phys. Lett. 83, 4897 (2003).

2002

U. K. Tirlapur and K. Konig, Nature 418, 290 (2002).

2001

A. A. Karabutov, E. V. Savateeva, and A. A. Oraevsky, Proc. SPIE 4256, 179 (2001).

1999

A. Takami, H. Kurita, and S. Koda, J. Phys. Chem. B 103, 1226 (1999).

1991

M. W. Berns, W. H. Wright, and R. W. Steubing, Int. Rev. Cytol. 129, 1 (1991).

1983

R. R. Anderson and J. A. Parrish, Science 220, 524 (1983).

Anderson, R. R.

C. M. Pitsillides, E. K. Joe, X. Wei, R. R. Anderson, and C. P. Lin, Biophysical Journal 84, 4023 (2003).

R. R. Anderson and J. A. Parrish, Science 220, 524 (1983).

Ashida, H.

Berns, M. W.

M. W. Berns, W. H. Wright, and R. W. Steubing, Int. Rev. Cytol. 129, 1 (1991).

Bu, M.

Y. Wang, L. Wang, X. Deng, Y. Liu, and M. Bu, Chinese J. Lasers (in Chinese) 35, 1491 (2008).

Dahmen, C.

A. Plech, V. Kotaidis, S. Gresillon, C. Dahmen, and G. von Plessen, Phys. Rev. B 70, 195423 (2004).

Deng, X.

Y. Wang, L. Wang, X. Deng, Y. Liu, and M. Bu, Chinese J. Lasers (in Chinese) 35, 1491 (2008).

Endl, E.

C. Yao, R. Rahmanzadeh, E. Endl, Z. Zhang, J. Gerdes, and G. Huttmann, J. Biomed. Opt. 10, 064012 (2005).

Galitovsky, V.

V. P. Zharov, V. Galitovsky, and M. Viegas, Appl. Phys. Lett. 83, 4897 (2003).

Gerdes, J.

C. Yao, R. Rahmanzadeh, E. Endl, Z. Zhang, J. Gerdes, and G. Huttmann, J. Biomed. Opt. 10, 064012 (2005).

Gresillon, S.

A. Plech, V. Kotaidis, S. Gresillon, C. Dahmen, and G. von Plessen, Phys. Rev. B 70, 195423 (2004).

Guo, C.

D. Wang, J. Yu, C. Guo, and W. Qi, Chinese J. Lasers (in Chinese) 35, 1579 (2008).

Huettmann, G.

C.-P. Yao, Z.-X. Zhang, R. Rahmanzadeh, and G. Huettmann, IEEE Trans. Nanobiosici. 7, 111 (2008).

Huttmann, G.

C. Yao, R. Rahmanzadeh, E. Endl, Z. Zhang, J. Gerdes, and G. Huttmann, J. Biomed. Opt. 10, 064012 (2005).

Joe, E. K.

C. M. Pitsillides, E. K. Joe, X. Wei, R. R. Anderson, and C. P. Lin, Biophysical Journal 84, 4023 (2003).

Karabutov, A. A.

A. A. Karabutov, E. V. Savateeva, and A. A. Oraevsky, Proc. SPIE 4256, 179 (2001).

Koda, S.

A. Takami, H. Kurita, and S. Koda, J. Phys. Chem. B 103, 1226 (1999).

Konig, K.

U. K. Tirlapur and K. Konig, Nature 418, 290 (2002).

Kotaidis, V.

A. Plech, V. Kotaidis, M. Lorenc, and M. Wulff, Chem. Phys. Lett. 401, 565 (2005).

A. Plech, V. Kotaidis, S. Gresillon, C. Dahmen, and G. von Plessen, Phys. Rev. B 70, 195423 (2004).

Kurita, H.

A. Takami, H. Kurita, and S. Koda, J. Phys. Chem. B 103, 1226 (1999).

Li, Z.

C. Yao, Z. Li, and Z. Zhang, Acta Opt. Sin. (in Chinese) 25, 1664 (2005).

Lin, C. P.

C. M. Pitsillides, E. K. Joe, X. Wei, R. R. Anderson, and C. P. Lin, Biophysical Journal 84, 4023 (2003).

Liu, Y.

Y. Wang, L. Wang, X. Deng, Y. Liu, and M. Bu, Chinese J. Lasers (in Chinese) 35, 1491 (2008).

Lorenc, M.

A. Plech, V. Kotaidis, M. Lorenc, and M. Wulff, Chem. Phys. Lett. 401, 565 (2005).

Masaki, Y.

Obara, M.

Ogura, M.

Oraevsky, A. A.

A. A. Karabutov, E. V. Savateeva, and A. A. Oraevsky, Proc. SPIE 4256, 179 (2001).

Parrish, J. A.

R. R. Anderson and J. A. Parrish, Science 220, 524 (1983).

Pitsillides, C. M.

C. M. Pitsillides, E. K. Joe, X. Wei, R. R. Anderson, and C. P. Lin, Biophysical Journal 84, 4023 (2003).

Plech, A.

A. Plech, V. Kotaidis, M. Lorenc, and M. Wulff, Chem. Phys. Lett. 401, 565 (2005).

A. Plech, V. Kotaidis, S. Gresillon, C. Dahmen, and G. von Plessen, Phys. Rev. B 70, 195423 (2004).

Plessen, G. von

A. Plech, V. Kotaidis, S. Gresillon, C. Dahmen, and G. von Plessen, Phys. Rev. B 70, 195423 (2004).

Qi, W.

D. Wang, J. Yu, C. Guo, and W. Qi, Chinese J. Lasers (in Chinese) 35, 1579 (2008).

Qu, X.

Rahmanzadeh, R.

C.-P. Yao, Z.-X. Zhang, R. Rahmanzadeh, and G. Huettmann, IEEE Trans. Nanobiosici. 7, 111 (2008).

C. Yao, R. Rahmanzadeh, E. Endl, Z. Zhang, J. Gerdes, and G. Huttmann, J. Biomed. Opt. 10, 064012 (2005).

Sato, S.

Savateeva, E. V.

A. A. Karabutov, E. V. Savateeva, and A. A. Oraevsky, Proc. SPIE 4256, 179 (2001).

Steubing, R. W.

M. W. Berns, W. H. Wright, and R. W. Steubing, Int. Rev. Cytol. 129, 1 (1991).

Takami, A.

A. Takami, H. Kurita, and S. Koda, J. Phys. Chem. B 103, 1226 (1999).

Terakawa, M.

Tirlapur, U. K.

U. K. Tirlapur and K. Konig, Nature 418, 290 (2002).

Uenoyama, M.

Viegas, M.

V. P. Zharov, V. Galitovsky, and M. Viegas, Appl. Phys. Lett. 83, 4897 (2003).

Wang, D.

D. Wang, J. Yu, C. Guo, and W. Qi, Chinese J. Lasers (in Chinese) 35, 1579 (2008).

Wang, J.

Wang, L.

Y. Wang, L. Wang, X. Deng, Y. Liu, and M. Bu, Chinese J. Lasers (in Chinese) 35, 1491 (2008).

Wang, Y.

Y. Wang, L. Wang, X. Deng, Y. Liu, and M. Bu, Chinese J. Lasers (in Chinese) 35, 1491 (2008).

Wei, X.

C. M. Pitsillides, E. K. Joe, X. Wei, R. R. Anderson, and C. P. Lin, Biophysical Journal 84, 4023 (2003).

Wright, W. H.

M. W. Berns, W. H. Wright, and R. W. Steubing, Int. Rev. Cytol. 129, 1 (1991).

Wulff, M.

A. Plech, V. Kotaidis, M. Lorenc, and M. Wulff, Chem. Phys. Lett. 401, 565 (2005).

Yao, C.

C. Yao and Z. Zhang, Acta Opt. Sin. (in Chinese) 29, 1610 (2009).

X. Qu, J. Wang, C. Yao, and Z. Zhang, Chin. Opt. Lett. 6, 879 (2008)

C. Yao, Z. Li, and Z. Zhang, Acta Opt. Sin. (in Chinese) 25, 1664 (2005).

C. Yao, R. Rahmanzadeh, E. Endl, Z. Zhang, J. Gerdes, and G. Huttmann, J. Biomed. Opt. 10, 064012 (2005).

Yao, C.-P.

C.-P. Yao, Z.-X. Zhang, R. Rahmanzadeh, and G. Huettmann, IEEE Trans. Nanobiosici. 7, 111 (2008).

Yu, J.

D. Wang, J. Yu, C. Guo, and W. Qi, Chinese J. Lasers (in Chinese) 35, 1579 (2008).

Zhang, Z.

C. Yao and Z. Zhang, Acta Opt. Sin. (in Chinese) 29, 1610 (2009).

X. Qu, J. Wang, C. Yao, and Z. Zhang, Chin. Opt. Lett. 6, 879 (2008)

C. Yao, Z. Li, and Z. Zhang, Acta Opt. Sin. (in Chinese) 25, 1664 (2005).

C. Yao, R. Rahmanzadeh, E. Endl, Z. Zhang, J. Gerdes, and G. Huttmann, J. Biomed. Opt. 10, 064012 (2005).

Zhang, Z.-X.

C.-P. Yao, Z.-X. Zhang, R. Rahmanzadeh, and G. Huettmann, IEEE Trans. Nanobiosici. 7, 111 (2008).

Zharov, V. P.

V. P. Zharov, V. Galitovsky, and M. Viegas, Appl. Phys. Lett. 83, 4897 (2003).

Acta Opt. Sin. (in Chinese)

C. Yao, Z. Li, and Z. Zhang, Acta Opt. Sin. (in Chinese) 25, 1664 (2005).

C. Yao and Z. Zhang, Acta Opt. Sin. (in Chinese) 29, 1610 (2009).

Appl. Phys. Lett.

V. P. Zharov, V. Galitovsky, and M. Viegas, Appl. Phys. Lett. 83, 4897 (2003).

Biophysical Journal

C. M. Pitsillides, E. K. Joe, X. Wei, R. R. Anderson, and C. P. Lin, Biophysical Journal 84, 4023 (2003).

Chem. Phys. Lett.

A. Plech, V. Kotaidis, M. Lorenc, and M. Wulff, Chem. Phys. Lett. 401, 565 (2005).

Chin. Opt. Lett.

Chinese J. Lasers (in Chinese)

D. Wang, J. Yu, C. Guo, and W. Qi, Chinese J. Lasers (in Chinese) 35, 1579 (2008).

Y. Wang, L. Wang, X. Deng, Y. Liu, and M. Bu, Chinese J. Lasers (in Chinese) 35, 1491 (2008).

IEEE Trans. Nanobiosici.

C.-P. Yao, Z.-X. Zhang, R. Rahmanzadeh, and G. Huettmann, IEEE Trans. Nanobiosici. 7, 111 (2008).

Int. Rev. Cytol.

M. W. Berns, W. H. Wright, and R. W. Steubing, Int. Rev. Cytol. 129, 1 (1991).

J. Biomed. Opt.

C. Yao, R. Rahmanzadeh, E. Endl, Z. Zhang, J. Gerdes, and G. Huttmann, J. Biomed. Opt. 10, 064012 (2005).

J. Phys. Chem. B

A. Takami, H. Kurita, and S. Koda, J. Phys. Chem. B 103, 1226 (1999).

Nature

U. K. Tirlapur and K. Konig, Nature 418, 290 (2002).

Opt. Lett.

Phys. Rev. B

A. Plech, V. Kotaidis, S. Gresillon, C. Dahmen, and G. von Plessen, Phys. Rev. B 70, 195423 (2004).

Proc. SPIE

A. A. Karabutov, E. V. Savateeva, and A. A. Oraevsky, Proc. SPIE 4256, 179 (2001).

Science

R. R. Anderson and J. A. Parrish, Science 220, 524 (1983).

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