Abstract

A·C mismatches are studied by Raman spectral characterization of PolyA, PolyC, and their equimolar complex in solution of 0.14 mol/L Na+, pH7.0. Experimental results show that A·C mismatches occur to be A/B (mainly A) conformers, and unlike Watson-Crick base pairing, this kind of mismatches is stabilized by only one hydrogen bond involving cytosine N4H2 and adenine N7. The formation of A·C complex makes the base stacking interactions much stronger, and conformation of the backbone more ordered, which leads to obvious Raman hypochromic effect with some shifts in corresponding bands.

© 2008 Chinese Optics Letters

PDF Article

References

  • View by:
  • |
  • |

  1. N. Peyret, P. A. Seneviratne, H. T. Allawi, and J. SantaLucia, Jr., Biochem. 38, 3468 (1999).
  2. R. J. Carter, K. J. Baeyens, J. SantaLucia, D. H. Turner, and S. R. Holbrook Nucleic Acids Res. 25, 4117 (1997).
  3. H. T. Allawi and J. SantaLucia, Jr., Biochem. 37, 2170 (1998).
  4. H. T. Allawi and J. SantaLucia, Jr., Biochem. 36, 10581 (1997).
  5. H. T. Allawi and J. SantaLucia, Jr., Nucleic Acids Res. 26, 4925 (1998).
  6. D. Gautheret, D. Konings, and R. R. Gutell, RNA 1, 807 (1995).
  7. G. Pieczenik, Biochem. Mol. Biol. Int. 5, 879 (1994).
  8. Y.-L. Tang and Z.-Y. Guo, Acta Biochim. Biophys. Sin. 37, 39 (2005).
  9. G. Liu, Y. Meng, S. Liu, and Y. Hu, Chin. Opt. Lett. 2, 154 (2004).
  10. G. J. Thomas, Jr. and M. Tsuboi, Adv. Biophys. Chem. 3, 1 (1993).
  11. B. Prescott, R. Gamache, J. Livramento, and G. J. Thomas, Jr., Biopolymers 13, 1821 (1974).
  12. B. Prescott, W. Steinmetz, and G. J. Thomas, Jr., Biopolymers 23, 235 (1984).
  13. E. W. Small and W. L. Peticolas, Biopolymers 10, 1377 (1971).
  14. S. C. Erfurth, E. J. Kiser, and W. L. Peticolas, Proc. Natl. Acad. Sci. USA 69, 938 (1972).
  15. P. Lagant, G. Vergoten, and W. L. Peticolas, J. Raman Spectrosc. 30, 1001 (1999).
  16. H. Deng, V. A. Bloomfield, J. M. Benevides, and G. J. Thomas, Jr., Biopolymers 50, 656 (1999).
  17. J. G. Duguid, V. A. Bloomfield, J. M. Benivides, and G. J. Thomas, Jr., Biophys. J. 69, 2623 (1995).
  18. J. G. Duguid, V. A. Bloomfield, J. M. Benivides, and G. J. Thomas, Jr., Biophys. J. 71, 3350 (1996).
  19. J. R. Ruiz-Chica, M. A. Medina, F. Sánchez-Jiménez, and F. J. Ramírez, J. Raman Spectrosc. 35, 93 (2004).
  20. J. R. Ruiz-Chica, M. A. Medina, F. Sánchez-Jiménez, and F. J. Ramírez, Biochim. Biophys. Acta 1628, 11 (2003).

2005 (1)

Y.-L. Tang and Z.-Y. Guo, Acta Biochim. Biophys. Sin. 37, 39 (2005).

2004 (2)

J. R. Ruiz-Chica, M. A. Medina, F. Sánchez-Jiménez, and F. J. Ramírez, J. Raman Spectrosc. 35, 93 (2004).

G. Liu, Y. Meng, S. Liu, and Y. Hu, Chin. Opt. Lett. 2, 154 (2004).

2003 (1)

J. R. Ruiz-Chica, M. A. Medina, F. Sánchez-Jiménez, and F. J. Ramírez, Biochim. Biophys. Acta 1628, 11 (2003).

1999 (3)

P. Lagant, G. Vergoten, and W. L. Peticolas, J. Raman Spectrosc. 30, 1001 (1999).

H. Deng, V. A. Bloomfield, J. M. Benevides, and G. J. Thomas, Jr., Biopolymers 50, 656 (1999).

N. Peyret, P. A. Seneviratne, H. T. Allawi, and J. SantaLucia, Jr., Biochem. 38, 3468 (1999).

1998 (2)

H. T. Allawi and J. SantaLucia, Jr., Biochem. 37, 2170 (1998).

H. T. Allawi and J. SantaLucia, Jr., Nucleic Acids Res. 26, 4925 (1998).

1997 (2)

H. T. Allawi and J. SantaLucia, Jr., Biochem. 36, 10581 (1997).

R. J. Carter, K. J. Baeyens, J. SantaLucia, D. H. Turner, and S. R. Holbrook Nucleic Acids Res. 25, 4117 (1997).

1996 (1)

J. G. Duguid, V. A. Bloomfield, J. M. Benivides, and G. J. Thomas, Jr., Biophys. J. 71, 3350 (1996).

1995 (2)

J. G. Duguid, V. A. Bloomfield, J. M. Benivides, and G. J. Thomas, Jr., Biophys. J. 69, 2623 (1995).

D. Gautheret, D. Konings, and R. R. Gutell, RNA 1, 807 (1995).

1994 (1)

G. Pieczenik, Biochem. Mol. Biol. Int. 5, 879 (1994).

1993 (1)

G. J. Thomas, Jr. and M. Tsuboi, Adv. Biophys. Chem. 3, 1 (1993).

1984 (1)

B. Prescott, W. Steinmetz, and G. J. Thomas, Jr., Biopolymers 23, 235 (1984).

1974 (1)

B. Prescott, R. Gamache, J. Livramento, and G. J. Thomas, Jr., Biopolymers 13, 1821 (1974).

1972 (1)

S. C. Erfurth, E. J. Kiser, and W. L. Peticolas, Proc. Natl. Acad. Sci. USA 69, 938 (1972).

1971 (1)

E. W. Small and W. L. Peticolas, Biopolymers 10, 1377 (1971).

Acta Biochim. Biophys. Sin. (1)

Y.-L. Tang and Z.-Y. Guo, Acta Biochim. Biophys. Sin. 37, 39 (2005).

Adv. Biophys. Chem. (1)

G. J. Thomas, Jr. and M. Tsuboi, Adv. Biophys. Chem. 3, 1 (1993).

and S. R. Holbrook Nucleic Acids Res. (1)

R. J. Carter, K. J. Baeyens, J. SantaLucia, D. H. Turner, and S. R. Holbrook Nucleic Acids Res. 25, 4117 (1997).

Biochem. (3)

H. T. Allawi and J. SantaLucia, Jr., Biochem. 37, 2170 (1998).

H. T. Allawi and J. SantaLucia, Jr., Biochem. 36, 10581 (1997).

N. Peyret, P. A. Seneviratne, H. T. Allawi, and J. SantaLucia, Jr., Biochem. 38, 3468 (1999).

Biochem. Mol. Biol. Int. (1)

G. Pieczenik, Biochem. Mol. Biol. Int. 5, 879 (1994).

Biochim. Biophys. Acta (1)

J. R. Ruiz-Chica, M. A. Medina, F. Sánchez-Jiménez, and F. J. Ramírez, Biochim. Biophys. Acta 1628, 11 (2003).

Biophys. J. (2)

J. G. Duguid, V. A. Bloomfield, J. M. Benivides, and G. J. Thomas, Jr., Biophys. J. 69, 2623 (1995).

J. G. Duguid, V. A. Bloomfield, J. M. Benivides, and G. J. Thomas, Jr., Biophys. J. 71, 3350 (1996).

Biopolymers (4)

H. Deng, V. A. Bloomfield, J. M. Benevides, and G. J. Thomas, Jr., Biopolymers 50, 656 (1999).

B. Prescott, R. Gamache, J. Livramento, and G. J. Thomas, Jr., Biopolymers 13, 1821 (1974).

B. Prescott, W. Steinmetz, and G. J. Thomas, Jr., Biopolymers 23, 235 (1984).

E. W. Small and W. L. Peticolas, Biopolymers 10, 1377 (1971).

Chin. Opt. Lett. (1)

J. Raman Spectrosc. (2)

J. R. Ruiz-Chica, M. A. Medina, F. Sánchez-Jiménez, and F. J. Ramírez, J. Raman Spectrosc. 35, 93 (2004).

P. Lagant, G. Vergoten, and W. L. Peticolas, J. Raman Spectrosc. 30, 1001 (1999).

Nucleic Acids Res. (1)

H. T. Allawi and J. SantaLucia, Jr., Nucleic Acids Res. 26, 4925 (1998).

Proc. Natl. Acad. Sci. USA (1)

S. C. Erfurth, E. J. Kiser, and W. L. Peticolas, Proc. Natl. Acad. Sci. USA 69, 938 (1972).

RNA (1)

D. Gautheret, D. Konings, and R. R. Gutell, RNA 1, 807 (1995).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.