Abstract

An optical biosensor with up-converting phosphor (UCP) marker is developed for the sensitive rapid immunoassay to the specific biomolecule. UCP can emit visible light when excited by infrared light. Through detecting and analyzing the content of UCP particles on the test strip after immunoreaction, the concentration of target analyte in the sample can be obtained. The detection sensitivity to plague IgG is better than 5 ng/ml; to plague FI-Ab is better than 100 pg/ml; to plague Yersinia pestis cell is better than 3*10^(4) CFU/ml. Good linear response characteristics and an excellent correlation (R2>=0.95) have been verified by quantitative detection results. In the practical application, detection results to 167 analytic samples have an excellent consistency with those obtained by reverse hemagglutination test. The up-converting phosphor technology (UPT) based biosensor has stable, reliable, and sensitive performances. It can meet the need of various bioassay applications.

© 2006 Chinese Optics Letters

PDF Article

References

  • View by:
  • |
  • |
  • |

  1. U. Bilitewski and A. P. F. Turner, Biosensors in Environmental Monitoring (Harwood Academic Publishers, Amsterdam, 2000).
  2. K. R. Rogers, A. Mulchandan, and W. Zhou, (eds.) Biosensor and Chemical Sensor Technology: Process Monitoring and Control (American Chemical Society, Washington DC, 1996).
  3. P. R. Mathewson and J. W. Finley, Biosensor Design and Application (American Chemical Society, Washington DC, 1992).
  4. L. R. Ma and Z. H. Jiang, Biochip (in Chinese) (Chemical Industry Press, Beijing, 2002).
  5. H. J. Zijlmans, J. Bonnet, J. Burton, K. Kardos, T. Vail, R. S. Niedbala, and H. J. Tanke, Analytical Biochemistry 267, 30 (1999).
  6. R. S. Niedbala, T. L. Vail, H. Feindt, S. Li, and J. Burton, Proc. SPIE 3913, 193 (2000).
  7. J. F. Suyver, J. Grimm, K. W. Kramer, and H. U. Gudel, Journal of Luminescence 114, 53 (2004).
  8. L. Zhou, J. Ji, and R. F. Yang, Biotechnology Information (in Chinese) 164, (4) 20 (2003).
  9. Y. K. Zhao, L. Zhou, H. J. Huang, R. F. Yang, L. H. Huang, B. Q. Ren, J. Lu, and X. Z. Wang, Acta Opt. Sin. (in Chinese) 25, 841 (2005).

2005 (1)

Y. K. Zhao, L. Zhou, H. J. Huang, R. F. Yang, L. H. Huang, B. Q. Ren, J. Lu, and X. Z. Wang, Acta Opt. Sin. (in Chinese) 25, 841 (2005).

2004 (1)

J. F. Suyver, J. Grimm, K. W. Kramer, and H. U. Gudel, Journal of Luminescence 114, 53 (2004).

2003 (1)

L. Zhou, J. Ji, and R. F. Yang, Biotechnology Information (in Chinese) 164, (4) 20 (2003).

2000 (1)

R. S. Niedbala, T. L. Vail, H. Feindt, S. Li, and J. Burton, Proc. SPIE 3913, 193 (2000).

1999 (1)

H. J. Zijlmans, J. Bonnet, J. Burton, K. Kardos, T. Vail, R. S. Niedbala, and H. J. Tanke, Analytical Biochemistry 267, 30 (1999).

Bonnet, J.

H. J. Zijlmans, J. Bonnet, J. Burton, K. Kardos, T. Vail, R. S. Niedbala, and H. J. Tanke, Analytical Biochemistry 267, 30 (1999).

Burton, J.

R. S. Niedbala, T. L. Vail, H. Feindt, S. Li, and J. Burton, Proc. SPIE 3913, 193 (2000).

H. J. Zijlmans, J. Bonnet, J. Burton, K. Kardos, T. Vail, R. S. Niedbala, and H. J. Tanke, Analytical Biochemistry 267, 30 (1999).

Feindt, H.

R. S. Niedbala, T. L. Vail, H. Feindt, S. Li, and J. Burton, Proc. SPIE 3913, 193 (2000).

Grimm, J.

J. F. Suyver, J. Grimm, K. W. Kramer, and H. U. Gudel, Journal of Luminescence 114, 53 (2004).

Gudel, H. U.

J. F. Suyver, J. Grimm, K. W. Kramer, and H. U. Gudel, Journal of Luminescence 114, 53 (2004).

Huang, H. J.

Y. K. Zhao, L. Zhou, H. J. Huang, R. F. Yang, L. H. Huang, B. Q. Ren, J. Lu, and X. Z. Wang, Acta Opt. Sin. (in Chinese) 25, 841 (2005).

Huang, L. H.

Y. K. Zhao, L. Zhou, H. J. Huang, R. F. Yang, L. H. Huang, B. Q. Ren, J. Lu, and X. Z. Wang, Acta Opt. Sin. (in Chinese) 25, 841 (2005).

Ji, J.

L. Zhou, J. Ji, and R. F. Yang, Biotechnology Information (in Chinese) 164, (4) 20 (2003).

Kardos, K.

H. J. Zijlmans, J. Bonnet, J. Burton, K. Kardos, T. Vail, R. S. Niedbala, and H. J. Tanke, Analytical Biochemistry 267, 30 (1999).

Kramer, K. W.

J. F. Suyver, J. Grimm, K. W. Kramer, and H. U. Gudel, Journal of Luminescence 114, 53 (2004).

Li, S.

R. S. Niedbala, T. L. Vail, H. Feindt, S. Li, and J. Burton, Proc. SPIE 3913, 193 (2000).

Lu, J.

Y. K. Zhao, L. Zhou, H. J. Huang, R. F. Yang, L. H. Huang, B. Q. Ren, J. Lu, and X. Z. Wang, Acta Opt. Sin. (in Chinese) 25, 841 (2005).

Niedbala, R. S.

R. S. Niedbala, T. L. Vail, H. Feindt, S. Li, and J. Burton, Proc. SPIE 3913, 193 (2000).

H. J. Zijlmans, J. Bonnet, J. Burton, K. Kardos, T. Vail, R. S. Niedbala, and H. J. Tanke, Analytical Biochemistry 267, 30 (1999).

Ren, B. Q.

Y. K. Zhao, L. Zhou, H. J. Huang, R. F. Yang, L. H. Huang, B. Q. Ren, J. Lu, and X. Z. Wang, Acta Opt. Sin. (in Chinese) 25, 841 (2005).

Suyver, J. F.

J. F. Suyver, J. Grimm, K. W. Kramer, and H. U. Gudel, Journal of Luminescence 114, 53 (2004).

Tanke, H. J.

H. J. Zijlmans, J. Bonnet, J. Burton, K. Kardos, T. Vail, R. S. Niedbala, and H. J. Tanke, Analytical Biochemistry 267, 30 (1999).

Vail, T.

H. J. Zijlmans, J. Bonnet, J. Burton, K. Kardos, T. Vail, R. S. Niedbala, and H. J. Tanke, Analytical Biochemistry 267, 30 (1999).

Vail, T. L.

R. S. Niedbala, T. L. Vail, H. Feindt, S. Li, and J. Burton, Proc. SPIE 3913, 193 (2000).

Wang, X. Z.

Y. K. Zhao, L. Zhou, H. J. Huang, R. F. Yang, L. H. Huang, B. Q. Ren, J. Lu, and X. Z. Wang, Acta Opt. Sin. (in Chinese) 25, 841 (2005).

Yang, R. F.

Y. K. Zhao, L. Zhou, H. J. Huang, R. F. Yang, L. H. Huang, B. Q. Ren, J. Lu, and X. Z. Wang, Acta Opt. Sin. (in Chinese) 25, 841 (2005).

L. Zhou, J. Ji, and R. F. Yang, Biotechnology Information (in Chinese) 164, (4) 20 (2003).

Zhao, Y. K.

Y. K. Zhao, L. Zhou, H. J. Huang, R. F. Yang, L. H. Huang, B. Q. Ren, J. Lu, and X. Z. Wang, Acta Opt. Sin. (in Chinese) 25, 841 (2005).

Zhou, L.

Y. K. Zhao, L. Zhou, H. J. Huang, R. F. Yang, L. H. Huang, B. Q. Ren, J. Lu, and X. Z. Wang, Acta Opt. Sin. (in Chinese) 25, 841 (2005).

L. Zhou, J. Ji, and R. F. Yang, Biotechnology Information (in Chinese) 164, (4) 20 (2003).

Zijlmans, H. J.

H. J. Zijlmans, J. Bonnet, J. Burton, K. Kardos, T. Vail, R. S. Niedbala, and H. J. Tanke, Analytical Biochemistry 267, 30 (1999).

Acta Opt. Sin. (in Chinese) (1)

Y. K. Zhao, L. Zhou, H. J. Huang, R. F. Yang, L. H. Huang, B. Q. Ren, J. Lu, and X. Z. Wang, Acta Opt. Sin. (in Chinese) 25, 841 (2005).

Analytical Biochemistry (1)

H. J. Zijlmans, J. Bonnet, J. Burton, K. Kardos, T. Vail, R. S. Niedbala, and H. J. Tanke, Analytical Biochemistry 267, 30 (1999).

Biotechnology Information (in Chinese) (1)

L. Zhou, J. Ji, and R. F. Yang, Biotechnology Information (in Chinese) 164, (4) 20 (2003).

Journal of Luminescence (1)

J. F. Suyver, J. Grimm, K. W. Kramer, and H. U. Gudel, Journal of Luminescence 114, 53 (2004).

Proc. SPIE (1)

R. S. Niedbala, T. L. Vail, H. Feindt, S. Li, and J. Burton, Proc. SPIE 3913, 193 (2000).

Other (4)

U. Bilitewski and A. P. F. Turner, Biosensors in Environmental Monitoring (Harwood Academic Publishers, Amsterdam, 2000).

K. R. Rogers, A. Mulchandan, and W. Zhou, (eds.) Biosensor and Chemical Sensor Technology: Process Monitoring and Control (American Chemical Society, Washington DC, 1996).

P. R. Mathewson and J. W. Finley, Biosensor Design and Application (American Chemical Society, Washington DC, 1992).

L. R. Ma and Z. H. Jiang, Biochip (in Chinese) (Chemical Industry Press, Beijing, 2002).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.