Abstract

The laser induced fluorescence (LIF) detection system for 5-um microspheres delivered on microfluidic chip is presented employing confocal optical scheme. The parameters of the optical system are specifically optimized for single microsphere detection. With the excitation laser spot size of 4.6 um and optical sectioning power of 27 um, the lowest concentration detection limit is 0.45 nmol/L, corresponding to only 122 molecules in probe volume. The microsphere detection is carried on successfully with the maximum signal-to-noise ratio (SNR) of 55.7, which provides good detection sensitivity.

© 2005 Chinese Optics Letters

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  1. D. A. A. Vignali, Journal of Immunological Methods 243, 243 (2000).
  2. R. Chen, L. Lowe, J. D. Wilson, E. Crowther, K. Tzeggai, J. E. Bishop, and R. Varro, Clin. Chem. 45, 1693 (1999).
  3. F. Lund-Johansen, K. Davis, J. Bishop, and R. de Waal Malefyt, Cytometry 39, 250 (2000).
  4. A. Spiro, M. Lowe, and D. Brown, Appl. Environ. Microbiol. 66, 4258 (2000).
  5. LiquiChip(TM) Applications Handbook (2002).
  6. T. Chovan and A. Guttman, Trends in Biotechnology 20, 116 (2002).
  7. J. N. McMullin, H. Qiao, S. Goel, and A. Grundmann, Rev. Sci. Instrum. 74, 4145 (2003).
  8. Y.-C. Tung, M. Zhang, C.-T. Lin, K. Kurabayashi, and S. J. Skerlos, Sensors and Actuators B 98, 356 (2004).
  9. H. Qiao, S. Goel, A. Grundmann, and J. N. McMullin, Proc. SPIE 4833, 54 (2003).
  10. G. Ocvirk, T. Tang, and D. J. Harrison, The Analyst 123, 1429 (1998).
  11. M. Gu, Principles of Three-Dimensional Imaging in Confocal Microscopes (in Chinese) G. Wang, Z. Chen, and L. Yang, (trans.) (New Times Press, Beijing, 2000).
  12. Z. Ding, Z. Bao, Y. Lin, and X. Bao, Chin. J. Lasers (in Chinese) 27, 531 (2000).

2004 (1)

Y.-C. Tung, M. Zhang, C.-T. Lin, K. Kurabayashi, and S. J. Skerlos, Sensors and Actuators B 98, 356 (2004).

2003 (2)

H. Qiao, S. Goel, A. Grundmann, and J. N. McMullin, Proc. SPIE 4833, 54 (2003).

J. N. McMullin, H. Qiao, S. Goel, and A. Grundmann, Rev. Sci. Instrum. 74, 4145 (2003).

2002 (1)

T. Chovan and A. Guttman, Trends in Biotechnology 20, 116 (2002).

2000 (4)

D. A. A. Vignali, Journal of Immunological Methods 243, 243 (2000).

F. Lund-Johansen, K. Davis, J. Bishop, and R. de Waal Malefyt, Cytometry 39, 250 (2000).

A. Spiro, M. Lowe, and D. Brown, Appl. Environ. Microbiol. 66, 4258 (2000).

Z. Ding, Z. Bao, Y. Lin, and X. Bao, Chin. J. Lasers (in Chinese) 27, 531 (2000).

1999 (1)

R. Chen, L. Lowe, J. D. Wilson, E. Crowther, K. Tzeggai, J. E. Bishop, and R. Varro, Clin. Chem. 45, 1693 (1999).

1998 (1)

G. Ocvirk, T. Tang, and D. J. Harrison, The Analyst 123, 1429 (1998).

Bao, X.

Z. Ding, Z. Bao, Y. Lin, and X. Bao, Chin. J. Lasers (in Chinese) 27, 531 (2000).

Bao, Z.

Z. Ding, Z. Bao, Y. Lin, and X. Bao, Chin. J. Lasers (in Chinese) 27, 531 (2000).

Bishop, J.

F. Lund-Johansen, K. Davis, J. Bishop, and R. de Waal Malefyt, Cytometry 39, 250 (2000).

Bishop, J. E.

R. Chen, L. Lowe, J. D. Wilson, E. Crowther, K. Tzeggai, J. E. Bishop, and R. Varro, Clin. Chem. 45, 1693 (1999).

Brown, D.

A. Spiro, M. Lowe, and D. Brown, Appl. Environ. Microbiol. 66, 4258 (2000).

Chen, R.

R. Chen, L. Lowe, J. D. Wilson, E. Crowther, K. Tzeggai, J. E. Bishop, and R. Varro, Clin. Chem. 45, 1693 (1999).

Chovan, T.

T. Chovan and A. Guttman, Trends in Biotechnology 20, 116 (2002).

Crowther, E.

R. Chen, L. Lowe, J. D. Wilson, E. Crowther, K. Tzeggai, J. E. Bishop, and R. Varro, Clin. Chem. 45, 1693 (1999).

Davis, K.

F. Lund-Johansen, K. Davis, J. Bishop, and R. de Waal Malefyt, Cytometry 39, 250 (2000).

Ding, Z.

Z. Ding, Z. Bao, Y. Lin, and X. Bao, Chin. J. Lasers (in Chinese) 27, 531 (2000).

Goel, S.

H. Qiao, S. Goel, A. Grundmann, and J. N. McMullin, Proc. SPIE 4833, 54 (2003).

J. N. McMullin, H. Qiao, S. Goel, and A. Grundmann, Rev. Sci. Instrum. 74, 4145 (2003).

Grundmann, A.

J. N. McMullin, H. Qiao, S. Goel, and A. Grundmann, Rev. Sci. Instrum. 74, 4145 (2003).

H. Qiao, S. Goel, A. Grundmann, and J. N. McMullin, Proc. SPIE 4833, 54 (2003).

Guttman, A.

T. Chovan and A. Guttman, Trends in Biotechnology 20, 116 (2002).

Harrison, D. J.

G. Ocvirk, T. Tang, and D. J. Harrison, The Analyst 123, 1429 (1998).

Kurabayashi, K.

Y.-C. Tung, M. Zhang, C.-T. Lin, K. Kurabayashi, and S. J. Skerlos, Sensors and Actuators B 98, 356 (2004).

Lin, C.-T.

Y.-C. Tung, M. Zhang, C.-T. Lin, K. Kurabayashi, and S. J. Skerlos, Sensors and Actuators B 98, 356 (2004).

Lin, Y.

Z. Ding, Z. Bao, Y. Lin, and X. Bao, Chin. J. Lasers (in Chinese) 27, 531 (2000).

Lowe, L.

R. Chen, L. Lowe, J. D. Wilson, E. Crowther, K. Tzeggai, J. E. Bishop, and R. Varro, Clin. Chem. 45, 1693 (1999).

Lowe, M.

A. Spiro, M. Lowe, and D. Brown, Appl. Environ. Microbiol. 66, 4258 (2000).

Lund-Johansen, F.

F. Lund-Johansen, K. Davis, J. Bishop, and R. de Waal Malefyt, Cytometry 39, 250 (2000).

Malefyt, R. de Waal

F. Lund-Johansen, K. Davis, J. Bishop, and R. de Waal Malefyt, Cytometry 39, 250 (2000).

McMullin, J. N.

J. N. McMullin, H. Qiao, S. Goel, and A. Grundmann, Rev. Sci. Instrum. 74, 4145 (2003).

H. Qiao, S. Goel, A. Grundmann, and J. N. McMullin, Proc. SPIE 4833, 54 (2003).

Ocvirk, G.

G. Ocvirk, T. Tang, and D. J. Harrison, The Analyst 123, 1429 (1998).

Qiao, H.

H. Qiao, S. Goel, A. Grundmann, and J. N. McMullin, Proc. SPIE 4833, 54 (2003).

J. N. McMullin, H. Qiao, S. Goel, and A. Grundmann, Rev. Sci. Instrum. 74, 4145 (2003).

Skerlos, S. J.

Y.-C. Tung, M. Zhang, C.-T. Lin, K. Kurabayashi, and S. J. Skerlos, Sensors and Actuators B 98, 356 (2004).

Spiro, A.

A. Spiro, M. Lowe, and D. Brown, Appl. Environ. Microbiol. 66, 4258 (2000).

Tang, T.

G. Ocvirk, T. Tang, and D. J. Harrison, The Analyst 123, 1429 (1998).

Tung, Y.-C.

Y.-C. Tung, M. Zhang, C.-T. Lin, K. Kurabayashi, and S. J. Skerlos, Sensors and Actuators B 98, 356 (2004).

Tzeggai, K.

R. Chen, L. Lowe, J. D. Wilson, E. Crowther, K. Tzeggai, J. E. Bishop, and R. Varro, Clin. Chem. 45, 1693 (1999).

Varro, R.

R. Chen, L. Lowe, J. D. Wilson, E. Crowther, K. Tzeggai, J. E. Bishop, and R. Varro, Clin. Chem. 45, 1693 (1999).

Vignali, D. A. A.

D. A. A. Vignali, Journal of Immunological Methods 243, 243 (2000).

Wilson, J. D.

R. Chen, L. Lowe, J. D. Wilson, E. Crowther, K. Tzeggai, J. E. Bishop, and R. Varro, Clin. Chem. 45, 1693 (1999).

Zhang, M.

Y.-C. Tung, M. Zhang, C.-T. Lin, K. Kurabayashi, and S. J. Skerlos, Sensors and Actuators B 98, 356 (2004).

Appl. Environ. Microbiol. (1)

A. Spiro, M. Lowe, and D. Brown, Appl. Environ. Microbiol. 66, 4258 (2000).

Chin. J. Lasers (in Chinese) (1)

Z. Ding, Z. Bao, Y. Lin, and X. Bao, Chin. J. Lasers (in Chinese) 27, 531 (2000).

Clin. Chem. (1)

R. Chen, L. Lowe, J. D. Wilson, E. Crowther, K. Tzeggai, J. E. Bishop, and R. Varro, Clin. Chem. 45, 1693 (1999).

Cytometry (1)

F. Lund-Johansen, K. Davis, J. Bishop, and R. de Waal Malefyt, Cytometry 39, 250 (2000).

Journal of Immunological Methods (1)

D. A. A. Vignali, Journal of Immunological Methods 243, 243 (2000).

Proc. SPIE (1)

H. Qiao, S. Goel, A. Grundmann, and J. N. McMullin, Proc. SPIE 4833, 54 (2003).

Rev. Sci. Instrum. (1)

J. N. McMullin, H. Qiao, S. Goel, and A. Grundmann, Rev. Sci. Instrum. 74, 4145 (2003).

Sensors and Actuators B (1)

Y.-C. Tung, M. Zhang, C.-T. Lin, K. Kurabayashi, and S. J. Skerlos, Sensors and Actuators B 98, 356 (2004).

The Analyst (1)

G. Ocvirk, T. Tang, and D. J. Harrison, The Analyst 123, 1429 (1998).

Trends in Biotechnology (1)

T. Chovan and A. Guttman, Trends in Biotechnology 20, 116 (2002).

Other (2)

LiquiChip(TM) Applications Handbook (2002).

M. Gu, Principles of Three-Dimensional Imaging in Confocal Microscopes (in Chinese) G. Wang, Z. Chen, and L. Yang, (trans.) (New Times Press, Beijing, 2000).

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