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Optica Publishing Group
  • Chinese Optics Letters
  • Vol. 4,
  • Issue 12,
  • pp. 722-724
  • (2006)

Methods of calibration to optical trapping force upon non-spherical cells

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Abstract

The dynamical equation of a trapping cell is solved to find calibration methods for the trapping force, and two methods are compared by synthetic experiment data. Results indicate that: Boltzmann distribution method (BDM) is available for the force calibration of non-spherical or anisotropic cells in arbitrary trap potential; the mean square displacement method (MSDM) is available only for a symmetric harmonic optical trap. The spatial resolution requirement of the calibration system is about a nanometer. The results agree with the reported experiments.

© 2006 Chinese Optics Letters

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