Abstract

The imaging technology of stimulated emission depletion (STED) utilizes the nonlinearity relationship between the fluorescence saturation and the excited state stimulated depletion. It implements three-dimensional (3D) imaging and breaks the diffraction barrier of far-field light microscopy by restricting fluorescent molecules at a sub-diffraction spot. In order to improve the resolution which attained by this technology, the computer simulation on temporal behavior of population probabilities of the sample was made in this paper, and the optimized parameters such as intensity, duration and delay time of the STED pulse were given.

© 2005 Chinese Optics Letters

Ultrahigh-contrast imaging by temporally modulated stimulated emission depletion

L.V. Doronina-Amitonova, I.V. Fedotov, and A.M. Zheltikov
Opt. Lett. 40(5) 725-728 (2015)

Nonlinear structured illumination microscopy by surface plasmon enhanced stimulated emission depletion

Han Zhang, Ming Zhao, and Leilei Peng
Opt. Express 19(24) 24783-24794 (2011)

Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging

Egidijus Auksorius, Bosanta R. Boruah, Christopher Dunsby, Peter M. P. Lanigan, Gordon Kennedy, Mark A. A. Neil, and Paul M. W. French
Opt. Lett. 33(2) 113-115 (2008)

The noise-limited-resolution for stimulated emission depletion microscopy of diffusing particles

Chris J. Lee and Klaus J. Boller
Opt. Express 20(12) 12793-12798 (2012)

Stimulated-emission-depletion microscopy with a multicolor stimulated-Raman-scattering light source

Brian R. Rankin, Robert R. Kellner, and Stefan W. Hell
Opt. Lett. 33(21) 2491-2493 (2008)