Abstract

Development of a compact fluorescence point-of-care system (POCS) is challenging. A prototype of compact fluorescence detection system and disposable microfluidic chip reported in this letter used a blue LED, 600-micron fiber, CCD detector, mylar and polymethyl-methacrylate (PMMA), and fluorescein solution as an excitation source, light-guide, detector, microfluidic chip substrates and samples, respectively. The results show the system is able to detect 0.01-1000 micro-g/l fluorescein in tris buffered saline pH 8.0 and gives linear response. This prototype can be used as a platform to develop a simple and compact multichannel-fluorescence point-of-care bioassay system with an inexpensive and disposable microfluidic chip.

© 2005 Chinese Optics Letters

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  1. P. N. Prasad, Introduction to Biophotonics (Wiley Interscience, New Jersey, 2003).
  2. J. R. Lakowics, Principles of Fluorescence Spectroscopy (Kluwer Academics, New York, 1999).
  3. K. R. Diamond, M. S. Patterson, and T. J. Farrell, App. Opt. 42, 2436 (2003).
  4. J. Khandurina and A. Guttman, J. Chromatography 943, 159 (2002).
  5. L. J. Kricka, Clin. Chim. Acta 307, 219 (2001).
  6. T. H. Schulte, R. L. Bardell, and B. H. Weigl, Clin. Chim. Acta 321, 1 (2002).
  7. F. Vinet, P. Chaton, and Y. Fouillet, Microelectronic Eng. 61-62, 41 (2002).
  8. P. Grodzinski, R. H. Liu, B. Chen, J. Blackwell, Y. Liu, D. Rhine, T. Smekal, D. Ganser, C. Romero, H. Yu, T. Chan, and Kroutchinina, Biomedical Microdevices 3-4, 275 (2001).
  9. J. Kameoka, H. G. Craighead, H. Zhang, and J. Henion, Anal. Chem. 73, 1935 (2001).
  10. B. H. Weigl, R. L. Bardell, T. Schulte, F. Battrell, and J. Hayenga, Biomedical Microdevices 3:4, 267 (2001).
  11. H. Klank, J. P. Kutter, and Geschke, Lab on a Chip 2, 242 (2002).
  12. J. Y. Cheng, C. W. Wei, K. H. Hsu, and T. H. Young, Sensors and Actuators B 99, 186 (2004).
  13. R. H. Kenneth, and Y. Paul, Lab on a Chip 3, 248 (2003).
  14. R. Irawan, S. C. Tjin, P. Yager, and D. W. Zhang, Biomedical Microdevices 7, 205 (2005).

2005 (1)

R. Irawan, S. C. Tjin, P. Yager, and D. W. Zhang, Biomedical Microdevices 7, 205 (2005).

2004 (1)

J. Y. Cheng, C. W. Wei, K. H. Hsu, and T. H. Young, Sensors and Actuators B 99, 186 (2004).

2003 (2)

R. H. Kenneth, and Y. Paul, Lab on a Chip 3, 248 (2003).

K. R. Diamond, M. S. Patterson, and T. J. Farrell, App. Opt. 42, 2436 (2003).

2002 (4)

J. Khandurina and A. Guttman, J. Chromatography 943, 159 (2002).

T. H. Schulte, R. L. Bardell, and B. H. Weigl, Clin. Chim. Acta 321, 1 (2002).

F. Vinet, P. Chaton, and Y. Fouillet, Microelectronic Eng. 61-62, 41 (2002).

H. Klank, J. P. Kutter, and Geschke, Lab on a Chip 2, 242 (2002).

2001 (4)

P. Grodzinski, R. H. Liu, B. Chen, J. Blackwell, Y. Liu, D. Rhine, T. Smekal, D. Ganser, C. Romero, H. Yu, T. Chan, and Kroutchinina, Biomedical Microdevices 3-4, 275 (2001).

J. Kameoka, H. G. Craighead, H. Zhang, and J. Henion, Anal. Chem. 73, 1935 (2001).

B. H. Weigl, R. L. Bardell, T. Schulte, F. Battrell, and J. Hayenga, Biomedical Microdevices 3:4, 267 (2001).

L. J. Kricka, Clin. Chim. Acta 307, 219 (2001).

Anal. Chem. (1)

J. Kameoka, H. G. Craighead, H. Zhang, and J. Henion, Anal. Chem. 73, 1935 (2001).

App. Opt. (1)

K. R. Diamond, M. S. Patterson, and T. J. Farrell, App. Opt. 42, 2436 (2003).

Biomedical Microdevices (2)

B. H. Weigl, R. L. Bardell, T. Schulte, F. Battrell, and J. Hayenga, Biomedical Microdevices 3:4, 267 (2001).

Biomedical Microdevices (1)

P. Grodzinski, R. H. Liu, B. Chen, J. Blackwell, Y. Liu, D. Rhine, T. Smekal, D. Ganser, C. Romero, H. Yu, T. Chan, and Kroutchinina, Biomedical Microdevices 3-4, 275 (2001).

Biomedical Microdevices (2)

R. Irawan, S. C. Tjin, P. Yager, and D. W. Zhang, Biomedical Microdevices 7, 205 (2005).

Clin. Chim. Acta (2)

L. J. Kricka, Clin. Chim. Acta 307, 219 (2001).

T. H. Schulte, R. L. Bardell, and B. H. Weigl, Clin. Chim. Acta 321, 1 (2002).

J. Chromatography (1)

J. Khandurina and A. Guttman, J. Chromatography 943, 159 (2002).

Lab on a Chip (2)

H. Klank, J. P. Kutter, and Geschke, Lab on a Chip 2, 242 (2002).

R. H. Kenneth, and Y. Paul, Lab on a Chip 3, 248 (2003).

Microelectronic Eng. (1)

F. Vinet, P. Chaton, and Y. Fouillet, Microelectronic Eng. 61-62, 41 (2002).

Sensors and Actuators B (1)

J. Y. Cheng, C. W. Wei, K. H. Hsu, and T. H. Young, Sensors and Actuators B 99, 186 (2004).

Other (2)

P. N. Prasad, Introduction to Biophotonics (Wiley Interscience, New Jersey, 2003).

J. R. Lakowics, Principles of Fluorescence Spectroscopy (Kluwer Academics, New York, 1999).

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