Abstract

The mechanical properties of fluorescent microtubules (MTs) are probed with dual-optical tweezers system. The results indicate that the fluorescent MTs are much easier to be extended compared with those without fluorescence. Such MT can be extended by 30% and force for breaking up it is only several piconewtons. Furthermore, we find that the breakup of the protofilaments is not simultaneous but step-by-step. Finally, the mechanism of the breaking is discussed.

© 2005 Chinese Optics Letters

PDF Article

References

  • View by:
  • |
  • |

  1. M. Yuan, P. J. Shaw, R. M. Warn, and C. W. Lloyd, Proc. Natl. Acad. Sci. 91, 6050 (1994).
  2. R. Yu, R. F. Huang, X. C. Wang, and M. Yuan, Protoplasma. 216, 113 (2001).
  3. P. J. de Pablo, I. A. Schaap, F. C. MacKintosh, and C. F. Schmidt, Phys. Rev. Lett. 91, 098101 (2003).
  4. J. W. Kerssemakers, M. E. Janson, A. van der Horst, and M. Dogterom, Appl. Phys. Lett. 83, 4441 (2003).
  5. M. Castoldi and A. V. Popov, Protein Expr. Purif. 32, 83 (2003).
  6. T. J. Keating, J. G. Peloquin, V. I. Rodionov, D. Momcilovic, and G. G. Borisy, PNAS 94, 5078 (1997).
  7. G. A. Vigers, M. Coue, and J. R. McIntosh, J. Cell Biol. 107, 1011 (1988).
  8. R. Dixit and R. Cyr, The Plant J. 36, 280 (2003).
  9. D. L. Sackett and R.E. Lippoldt, Biochem. 30, 3511 (1991).
  10. R. B. Dye, S. P. Fink, and R. C. Williams Jr., J. Biol. Chem. 268, 6847 (1993).

2003 (4)

P. J. de Pablo, I. A. Schaap, F. C. MacKintosh, and C. F. Schmidt, Phys. Rev. Lett. 91, 098101 (2003).

J. W. Kerssemakers, M. E. Janson, A. van der Horst, and M. Dogterom, Appl. Phys. Lett. 83, 4441 (2003).

M. Castoldi and A. V. Popov, Protein Expr. Purif. 32, 83 (2003).

R. Dixit and R. Cyr, The Plant J. 36, 280 (2003).

2001 (1)

R. Yu, R. F. Huang, X. C. Wang, and M. Yuan, Protoplasma. 216, 113 (2001).

1997 (1)

T. J. Keating, J. G. Peloquin, V. I. Rodionov, D. Momcilovic, and G. G. Borisy, PNAS 94, 5078 (1997).

1994 (1)

M. Yuan, P. J. Shaw, R. M. Warn, and C. W. Lloyd, Proc. Natl. Acad. Sci. 91, 6050 (1994).

1993 (1)

R. B. Dye, S. P. Fink, and R. C. Williams Jr., J. Biol. Chem. 268, 6847 (1993).

1991 (1)

D. L. Sackett and R.E. Lippoldt, Biochem. 30, 3511 (1991).

1988 (1)

G. A. Vigers, M. Coue, and J. R. McIntosh, J. Cell Biol. 107, 1011 (1988).

Appl. Phys. Lett. (1)

J. W. Kerssemakers, M. E. Janson, A. van der Horst, and M. Dogterom, Appl. Phys. Lett. 83, 4441 (2003).

Biochem. (1)

D. L. Sackett and R.E. Lippoldt, Biochem. 30, 3511 (1991).

J. Biol. Chem. (1)

R. B. Dye, S. P. Fink, and R. C. Williams Jr., J. Biol. Chem. 268, 6847 (1993).

J. Cell Biol. (1)

G. A. Vigers, M. Coue, and J. R. McIntosh, J. Cell Biol. 107, 1011 (1988).

Phys. Rev. Lett. (1)

P. J. de Pablo, I. A. Schaap, F. C. MacKintosh, and C. F. Schmidt, Phys. Rev. Lett. 91, 098101 (2003).

PNAS (1)

T. J. Keating, J. G. Peloquin, V. I. Rodionov, D. Momcilovic, and G. G. Borisy, PNAS 94, 5078 (1997).

Proc. Natl. Acad. Sci. (1)

M. Yuan, P. J. Shaw, R. M. Warn, and C. W. Lloyd, Proc. Natl. Acad. Sci. 91, 6050 (1994).

Protein Expr. Purif. (1)

M. Castoldi and A. V. Popov, Protein Expr. Purif. 32, 83 (2003).

Protoplasma. (1)

R. Yu, R. F. Huang, X. C. Wang, and M. Yuan, Protoplasma. 216, 113 (2001).

The Plant J. (1)

R. Dixit and R. Cyr, The Plant J. 36, 280 (2003).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.