Abstract

Electroporation is a physical process that transiently permeabilizes prokaryotic and eukaryotic cell membranes with an electrical pulse, permitting cell uptake of a variety of biological molecules. Because of the advantages of high efficiency, low toxicity popularity, and controllability, it has been applied in the fields such as cell engineering, genetic engineering, and so on. The principles and characteristics of gene transfection apparatus which is made by us will be illustrated in this paper. The temperature influence is considered and the optical fiber sensor in the apparatus is proposed.

© 2005 Chinese Optics Letters

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  1. J. C. Weaver, IEEE Trans. Plasma Sci. 28, 24 (2000).
  2. Y. Mounermer and P. F. Tosi, Biochem. Biophys. Acta 1027, 53 (1990).
  3. T. Kotnik, Bioelectrochemistry and Bioenergetics 43, 285 (1997).
  4. M. P. Saravolac, in Proceedings of IEE Colloquium on Condition Monitoring and Remanent Life Assessment in Power Transformers 1994 7 (1994).
  5. Y. B. Guo and G. E. Huang, Optics and Precision Engineering (in Chinese) 7, 31 (1999).

2000 (1)

J. C. Weaver, IEEE Trans. Plasma Sci. 28, 24 (2000).

1999 (1)

Y. B. Guo and G. E. Huang, Optics and Precision Engineering (in Chinese) 7, 31 (1999).

1997 (1)

T. Kotnik, Bioelectrochemistry and Bioenergetics 43, 285 (1997).

1990 (1)

Y. Mounermer and P. F. Tosi, Biochem. Biophys. Acta 1027, 53 (1990).

Biochem. Biophys. Acta (1)

Y. Mounermer and P. F. Tosi, Biochem. Biophys. Acta 1027, 53 (1990).

Bioelectrochemistry and Bioenergetics (1)

T. Kotnik, Bioelectrochemistry and Bioenergetics 43, 285 (1997).

IEEE Trans. Plasma Sci. (1)

J. C. Weaver, IEEE Trans. Plasma Sci. 28, 24 (2000).

Optics and Precision Engineering (in Chinese) (1)

Y. B. Guo and G. E. Huang, Optics and Precision Engineering (in Chinese) 7, 31 (1999).

Other (1)

M. P. Saravolac, in Proceedings of IEE Colloquium on Condition Monitoring and Remanent Life Assessment in Power Transformers 1994 7 (1994).

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