Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Chinese Optics Letters
  • Vol. 3,
  • Issue S1,
  • pp. S143-S146
  • (2005)

Confocal microscopy of FM 4-64 tagged membranes in the living fungus Trichoderma reesei

Not Accessible

Your library or personal account may give you access

Abstract

Visualization of the protein secretory pathway in the high secreting trichoderma reesei (T. reesei) hyphae using confocal microscopy will lead to better understanding of the cellular mechanisms involved in protein secretion and contribute to the identification of bottlenecks in the secretion of foreign proteins from fungi. An introduction into visualization approaches involved the application of the fluorescent dye FM 4-64 for staining of membrane-based structures in T. reesei hyphae. Confocal microscopy studies were carried out with 24-hour old cultures of the T. reesei strain Rut-C30. Staining of the fungal hyphae and characteristics of FM 4-64 including spectral properties, time course of the labeling and double labeling with other specific organelle dyes will be discussed.

© 2005 Chinese Optics Letters

PDF Article
More Like This
Confocal diffraction phase microscopy of live cells

Niyom Lue, Wonshik Choi, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld, and Gabriel Popescu
Opt. Lett. 33(18) 2074-2076 (2008)

Confocal supercritical angle microscopy for cell membrane imaging

Siddharth Sivankutty, Thomas Barroca, Céline Mayet, Guillaume Dupuis, Emmanuel Fort, and Sandrine Lévêque-Fort
Opt. Lett. 39(3) 555-558 (2014)

Spinning disk interferometric scattering confocal microscopy captures millisecond timescale dynamics of living cells

Yi-Teng Hsiao, Tsai-Ying Wu, Bo-Kuan Wu, Shi-Wei Chu, and Chia-Lung Hsieh
Opt. Express 30(25) 45233-45245 (2022)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved