We establish a system to measure the functional absorption cross section of photosystem II (PSII) (σPSII) and maximum quantum yield of photochemistry in PSII (Fv/Fm). The system utilizes a sequence of high-frequency excitation flashes at microsecond intervals to induce a microsecond-level fluorescence yield curve. Parameters σPSII and Fv/Fm are calculated by fitting the curve using nonlinear regression. Experimental results show that the relative standard deviation (RSD) of the system is less than 3%, and the correlation coefficient of Fv/Fm values measured by this system and those measured by pulse amplitude modulation method is 0.950.
© 2014 Chinese Optics LettersPDF Article