Abstract

We establish a system to measure the functional absorption cross section of photosystem II (PSII) (σ<sub>PSII</sub>) and maximum quantum yield of photochemistry in PSII (<i>F</i><sub>v</sub>/<i>F</i><sub>m</sub>). The system utilizes a sequence of high-frequency excitation flashes at microsecond intervals to induce a microsecond-level fluorescence yield curve. Parameters σ<sub>PSII</sub> and <i>F</i><sub>v</sub>/<i>F</i><sub>m</sub> are calculated by fitting the curve using nonlinear regression. Experimental results show that the relative standard deviation (RSD) of the system is less than 3%, and the correlation coefficient of <i>F</i><sub>v</sub>/<i>F</i><sub>m</sub> values measured by this system and those measured by pulse amplitude modulation method is 0.950.

© 2014 Chinese Optics Letters

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  1. B. Han, Z. Han, and X. Fu, Algae Photosynthesis: Mechanism and Models (Science Press, Beijing, 2003).
  2. D. J. Suggett, O. Prasil, and M. A. Borowitzka, Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications (Springer, 2011).
  3. B. Ke, Photosynthesis Photo Biochemistry and Photo Biophysics (Springer, 2001).
  4. W. O. Smith Jr., S. Tozzi, M. C. Long, P. N. Sedwick, J. A. Peloquin, R. B. Dunbar, D. A. Hutchins, Z. Kolber, and G. R. DiTullio, Deep Sea Res. Oceanogr. Res. Paper. 79, 141 (2013).
  5. S. T. Wilson, Z. S. Kolber, S. Tozzi, J. P. Zehr, and D. M. Karl, J. Phycol. 48, 595 (2012).
  6. Z. S. Kolber and P. G. Falkowski, Limnol. Oceanogr. 38, 1646 (1993).
  7. M. C. Perron, B. S. Qiu, N. Boucher, F. Bellemare, and P. Juneau, Toxicon 59, 567 (2012).
  8. K. Oxborough, C. M. Moore, D. J. Suggett, T. Lawson, H. G. Chan, and R. J. Geider, Limnol. Oceanogr. Methods 10, 142 (2012).
  9. P. J. Zarco-Tejada, A. Catalina, M. R. Gonzalez, and P. Martin, Rem. Sens. Environ. 136, 247 (2013).
  10. D. Mauzeral, Proc. Natl. Acad. Sci. Unit. States Am. 69, 1358 (1972).
  11. U. Schreiber, in Current Topics in Photosynthesis J. Amesz, A. J. Hoff, and H. J. Van Gorkum, (eds.) (Springer, Netherlands, 1986) pp. 259-270.
  12. Z. S. Kolber and P. G. Falkowski, in Proceedings of Oceans'92, Newport, RI 637 (1992).
  13. P. G. Falkowski and Z. S. Kolber, "Fast repetition rate (FRR) fluoroeter and method for measuring fluorescence and photosynthetic parameters." U. S. Patent 5, 426, 306(1995).
  14. Z. S. Kolber, O. Prasil, and P. G. Falkowski, Biochim. Biophys. Acta Bioenerg. 1367, 88 (1998).
  15. Z. Liu, P. Liu, and F. Yu, Chin. Opt. Lett. 10, 112201 (2012).
  16. Y. Wang, B. Zhao, Z. Zhuo, J. Wang, L. Zhang, H. Qin, and J. Chen, Chin. Opt. Lett. 11, 121404 (2013).
  17. J. Ling,"The Influence of Environmental Stress on the Growth and Photosynthesis of Chlorella pyrenoidosa", MS Thesis (East China Normal University, 2009).
  18. L. Chen, Q. Zheng, and Z. Liu, Ecology and Environmental Sciences (in Chinese) 18, 1231 (2009).
  19. X. Fu, "Studies on photosynthesis of phytoplankton in China Sea", PhD. Thesis (Graduate School of Chinese Academy of Sciences, 2007).
  20. U. Schreiber, C. Neubauer, and U. Schliwa, Photosynth. Res. 36, 65 (1993).
  21. Z. Kolber, J. Zehr, and P. Falkowski, Plant Physiol. 88, 923 (1988).

2013 (3)

W. O. Smith Jr., S. Tozzi, M. C. Long, P. N. Sedwick, J. A. Peloquin, R. B. Dunbar, D. A. Hutchins, Z. Kolber, and G. R. DiTullio, Deep Sea Res. Oceanogr. Res. Paper. 79, 141 (2013).

P. J. Zarco-Tejada, A. Catalina, M. R. Gonzalez, and P. Martin, Rem. Sens. Environ. 136, 247 (2013).

Y. Wang, B. Zhao, Z. Zhuo, J. Wang, L. Zhang, H. Qin, and J. Chen, Chin. Opt. Lett. 11, 121404 (2013).

2012 (4)

Z. Liu, P. Liu, and F. Yu, Chin. Opt. Lett. 10, 112201 (2012).

S. T. Wilson, Z. S. Kolber, S. Tozzi, J. P. Zehr, and D. M. Karl, J. Phycol. 48, 595 (2012).

M. C. Perron, B. S. Qiu, N. Boucher, F. Bellemare, and P. Juneau, Toxicon 59, 567 (2012).

K. Oxborough, C. M. Moore, D. J. Suggett, T. Lawson, H. G. Chan, and R. J. Geider, Limnol. Oceanogr. Methods 10, 142 (2012).

2009 (1)

L. Chen, Q. Zheng, and Z. Liu, Ecology and Environmental Sciences (in Chinese) 18, 1231 (2009).

1998 (1)

Z. S. Kolber, O. Prasil, and P. G. Falkowski, Biochim. Biophys. Acta Bioenerg. 1367, 88 (1998).

1995 (1)

P. G. Falkowski and Z. S. Kolber, "Fast repetition rate (FRR) fluoroeter and method for measuring fluorescence and photosynthetic parameters." U. S. Patent 5, 426, 306(1995).

1993 (2)

Z. S. Kolber and P. G. Falkowski, Limnol. Oceanogr. 38, 1646 (1993).

U. Schreiber, C. Neubauer, and U. Schliwa, Photosynth. Res. 36, 65 (1993).

1988 (1)

Z. Kolber, J. Zehr, and P. Falkowski, Plant Physiol. 88, 923 (1988).

1972 (1)

D. Mauzeral, Proc. Natl. Acad. Sci. Unit. States Am. 69, 1358 (1972).

Bellemare, F.

M. C. Perron, B. S. Qiu, N. Boucher, F. Bellemare, and P. Juneau, Toxicon 59, 567 (2012).

Boucher, N.

M. C. Perron, B. S. Qiu, N. Boucher, F. Bellemare, and P. Juneau, Toxicon 59, 567 (2012).

Catalina, A.

P. J. Zarco-Tejada, A. Catalina, M. R. Gonzalez, and P. Martin, Rem. Sens. Environ. 136, 247 (2013).

Chan, H. G.

K. Oxborough, C. M. Moore, D. J. Suggett, T. Lawson, H. G. Chan, and R. J. Geider, Limnol. Oceanogr. Methods 10, 142 (2012).

Chen, J.

Chen, L.

L. Chen, Q. Zheng, and Z. Liu, Ecology and Environmental Sciences (in Chinese) 18, 1231 (2009).

DiTullio, G. R.

W. O. Smith Jr., S. Tozzi, M. C. Long, P. N. Sedwick, J. A. Peloquin, R. B. Dunbar, D. A. Hutchins, Z. Kolber, and G. R. DiTullio, Deep Sea Res. Oceanogr. Res. Paper. 79, 141 (2013).

Dunbar, R. B.

W. O. Smith Jr., S. Tozzi, M. C. Long, P. N. Sedwick, J. A. Peloquin, R. B. Dunbar, D. A. Hutchins, Z. Kolber, and G. R. DiTullio, Deep Sea Res. Oceanogr. Res. Paper. 79, 141 (2013).

Falkowski, P.

Z. Kolber, J. Zehr, and P. Falkowski, Plant Physiol. 88, 923 (1988).

Falkowski, P. G.

Z. S. Kolber, O. Prasil, and P. G. Falkowski, Biochim. Biophys. Acta Bioenerg. 1367, 88 (1998).

P. G. Falkowski and Z. S. Kolber, "Fast repetition rate (FRR) fluoroeter and method for measuring fluorescence and photosynthetic parameters." U. S. Patent 5, 426, 306(1995).

Z. S. Kolber and P. G. Falkowski, Limnol. Oceanogr. 38, 1646 (1993).

Geider, R. J.

K. Oxborough, C. M. Moore, D. J. Suggett, T. Lawson, H. G. Chan, and R. J. Geider, Limnol. Oceanogr. Methods 10, 142 (2012).

Gonzalez, M. R.

P. J. Zarco-Tejada, A. Catalina, M. R. Gonzalez, and P. Martin, Rem. Sens. Environ. 136, 247 (2013).

Hutchins, D. A.

W. O. Smith Jr., S. Tozzi, M. C. Long, P. N. Sedwick, J. A. Peloquin, R. B. Dunbar, D. A. Hutchins, Z. Kolber, and G. R. DiTullio, Deep Sea Res. Oceanogr. Res. Paper. 79, 141 (2013).

Juneau, P.

M. C. Perron, B. S. Qiu, N. Boucher, F. Bellemare, and P. Juneau, Toxicon 59, 567 (2012).

Karl, D. M.

S. T. Wilson, Z. S. Kolber, S. Tozzi, J. P. Zehr, and D. M. Karl, J. Phycol. 48, 595 (2012).

Kolber, Z.

W. O. Smith Jr., S. Tozzi, M. C. Long, P. N. Sedwick, J. A. Peloquin, R. B. Dunbar, D. A. Hutchins, Z. Kolber, and G. R. DiTullio, Deep Sea Res. Oceanogr. Res. Paper. 79, 141 (2013).

Z. Kolber, J. Zehr, and P. Falkowski, Plant Physiol. 88, 923 (1988).

Kolber, Z. S.

S. T. Wilson, Z. S. Kolber, S. Tozzi, J. P. Zehr, and D. M. Karl, J. Phycol. 48, 595 (2012).

Z. S. Kolber, O. Prasil, and P. G. Falkowski, Biochim. Biophys. Acta Bioenerg. 1367, 88 (1998).

P. G. Falkowski and Z. S. Kolber, "Fast repetition rate (FRR) fluoroeter and method for measuring fluorescence and photosynthetic parameters." U. S. Patent 5, 426, 306(1995).

Z. S. Kolber and P. G. Falkowski, Limnol. Oceanogr. 38, 1646 (1993).

Lawson, T.

K. Oxborough, C. M. Moore, D. J. Suggett, T. Lawson, H. G. Chan, and R. J. Geider, Limnol. Oceanogr. Methods 10, 142 (2012).

Liu, P.

Liu, Z.

Z. Liu, P. Liu, and F. Yu, Chin. Opt. Lett. 10, 112201 (2012).

L. Chen, Q. Zheng, and Z. Liu, Ecology and Environmental Sciences (in Chinese) 18, 1231 (2009).

Long, M. C.

W. O. Smith Jr., S. Tozzi, M. C. Long, P. N. Sedwick, J. A. Peloquin, R. B. Dunbar, D. A. Hutchins, Z. Kolber, and G. R. DiTullio, Deep Sea Res. Oceanogr. Res. Paper. 79, 141 (2013).

Martin, P.

P. J. Zarco-Tejada, A. Catalina, M. R. Gonzalez, and P. Martin, Rem. Sens. Environ. 136, 247 (2013).

Mauzeral, D.

D. Mauzeral, Proc. Natl. Acad. Sci. Unit. States Am. 69, 1358 (1972).

Moore, C. M.

K. Oxborough, C. M. Moore, D. J. Suggett, T. Lawson, H. G. Chan, and R. J. Geider, Limnol. Oceanogr. Methods 10, 142 (2012).

Neubauer, C.

U. Schreiber, C. Neubauer, and U. Schliwa, Photosynth. Res. 36, 65 (1993).

Oxborough, K.

K. Oxborough, C. M. Moore, D. J. Suggett, T. Lawson, H. G. Chan, and R. J. Geider, Limnol. Oceanogr. Methods 10, 142 (2012).

Peloquin, J. A.

W. O. Smith Jr., S. Tozzi, M. C. Long, P. N. Sedwick, J. A. Peloquin, R. B. Dunbar, D. A. Hutchins, Z. Kolber, and G. R. DiTullio, Deep Sea Res. Oceanogr. Res. Paper. 79, 141 (2013).

Perron, M. C.

M. C. Perron, B. S. Qiu, N. Boucher, F. Bellemare, and P. Juneau, Toxicon 59, 567 (2012).

Prasil, O.

Z. S. Kolber, O. Prasil, and P. G. Falkowski, Biochim. Biophys. Acta Bioenerg. 1367, 88 (1998).

Qin, H.

Qiu, B. S.

M. C. Perron, B. S. Qiu, N. Boucher, F. Bellemare, and P. Juneau, Toxicon 59, 567 (2012).

Schliwa, U.

U. Schreiber, C. Neubauer, and U. Schliwa, Photosynth. Res. 36, 65 (1993).

Schreiber, U.

U. Schreiber, C. Neubauer, and U. Schliwa, Photosynth. Res. 36, 65 (1993).

Sedwick, P. N.

W. O. Smith Jr., S. Tozzi, M. C. Long, P. N. Sedwick, J. A. Peloquin, R. B. Dunbar, D. A. Hutchins, Z. Kolber, and G. R. DiTullio, Deep Sea Res. Oceanogr. Res. Paper. 79, 141 (2013).

Smith, Jr., W. O.

W. O. Smith Jr., S. Tozzi, M. C. Long, P. N. Sedwick, J. A. Peloquin, R. B. Dunbar, D. A. Hutchins, Z. Kolber, and G. R. DiTullio, Deep Sea Res. Oceanogr. Res. Paper. 79, 141 (2013).

Suggett, D. J.

K. Oxborough, C. M. Moore, D. J. Suggett, T. Lawson, H. G. Chan, and R. J. Geider, Limnol. Oceanogr. Methods 10, 142 (2012).

Tozzi, S.

W. O. Smith Jr., S. Tozzi, M. C. Long, P. N. Sedwick, J. A. Peloquin, R. B. Dunbar, D. A. Hutchins, Z. Kolber, and G. R. DiTullio, Deep Sea Res. Oceanogr. Res. Paper. 79, 141 (2013).

S. T. Wilson, Z. S. Kolber, S. Tozzi, J. P. Zehr, and D. M. Karl, J. Phycol. 48, 595 (2012).

Wang, J.

Wang, Y.

Wilson, S. T.

S. T. Wilson, Z. S. Kolber, S. Tozzi, J. P. Zehr, and D. M. Karl, J. Phycol. 48, 595 (2012).

Yu, F.

Zarco-Tejada, P. J.

P. J. Zarco-Tejada, A. Catalina, M. R. Gonzalez, and P. Martin, Rem. Sens. Environ. 136, 247 (2013).

Zehr, J.

Z. Kolber, J. Zehr, and P. Falkowski, Plant Physiol. 88, 923 (1988).

Zehr, J. P.

S. T. Wilson, Z. S. Kolber, S. Tozzi, J. P. Zehr, and D. M. Karl, J. Phycol. 48, 595 (2012).

Zhang, L.

Zhao, B.

Zheng, Q.

L. Chen, Q. Zheng, and Z. Liu, Ecology and Environmental Sciences (in Chinese) 18, 1231 (2009).

Zhuo, Z.

Biochim. Biophys. Acta Bioenerg. (1)

Z. S. Kolber, O. Prasil, and P. G. Falkowski, Biochim. Biophys. Acta Bioenerg. 1367, 88 (1998).

Chin. Opt. Lett. (2)

Deep Sea Res. Oceanogr. Res. Paper. (1)

W. O. Smith Jr., S. Tozzi, M. C. Long, P. N. Sedwick, J. A. Peloquin, R. B. Dunbar, D. A. Hutchins, Z. Kolber, and G. R. DiTullio, Deep Sea Res. Oceanogr. Res. Paper. 79, 141 (2013).

Ecology and Environmental Sciences (in Chinese) (1)

L. Chen, Q. Zheng, and Z. Liu, Ecology and Environmental Sciences (in Chinese) 18, 1231 (2009).

J. Phycol. (1)

S. T. Wilson, Z. S. Kolber, S. Tozzi, J. P. Zehr, and D. M. Karl, J. Phycol. 48, 595 (2012).

Limnol. Oceanogr. (1)

Z. S. Kolber and P. G. Falkowski, Limnol. Oceanogr. 38, 1646 (1993).

Limnol. Oceanogr. Methods (1)

K. Oxborough, C. M. Moore, D. J. Suggett, T. Lawson, H. G. Chan, and R. J. Geider, Limnol. Oceanogr. Methods 10, 142 (2012).

Photosynth. Res. (1)

U. Schreiber, C. Neubauer, and U. Schliwa, Photosynth. Res. 36, 65 (1993).

Plant Physiol. (1)

Z. Kolber, J. Zehr, and P. Falkowski, Plant Physiol. 88, 923 (1988).

Proc. Natl. Acad. Sci. Unit. States Am. (1)

D. Mauzeral, Proc. Natl. Acad. Sci. Unit. States Am. 69, 1358 (1972).

Rem. Sens. Environ. (1)

P. J. Zarco-Tejada, A. Catalina, M. R. Gonzalez, and P. Martin, Rem. Sens. Environ. 136, 247 (2013).

Toxicon (1)

M. C. Perron, B. S. Qiu, N. Boucher, F. Bellemare, and P. Juneau, Toxicon 59, 567 (2012).

U. S. Patent 5 (1)

P. G. Falkowski and Z. S. Kolber, "Fast repetition rate (FRR) fluoroeter and method for measuring fluorescence and photosynthetic parameters." U. S. Patent 5, 426, 306(1995).

Other (7)

B. Han, Z. Han, and X. Fu, Algae Photosynthesis: Mechanism and Models (Science Press, Beijing, 2003).

D. J. Suggett, O. Prasil, and M. A. Borowitzka, Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications (Springer, 2011).

B. Ke, Photosynthesis Photo Biochemistry and Photo Biophysics (Springer, 2001).

U. Schreiber, in Current Topics in Photosynthesis J. Amesz, A. J. Hoff, and H. J. Van Gorkum, (eds.) (Springer, Netherlands, 1986) pp. 259-270.

Z. S. Kolber and P. G. Falkowski, in Proceedings of Oceans'92, Newport, RI 637 (1992).

J. Ling,"The Influence of Environmental Stress on the Growth and Photosynthesis of Chlorella pyrenoidosa", MS Thesis (East China Normal University, 2009).

X. Fu, "Studies on photosynthesis of phytoplankton in China Sea", PhD. Thesis (Graduate School of Chinese Academy of Sciences, 2007).

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