Abstract
In the past two decades, two-photon microscopy (TPM) transforms biomedical research,
allowing nondestructive high-resolution fluorescent molecular imaging and label-free imaging in vivo
and in real time. Here we review the recent advances of TPM technology including novel laser
sources, new image acquisition paradigms, and microendoscopic imaging systems. Then, we survey the
capabilities of TPM imaging of biological relevant molecules such as nicotinamide adenine
dinucleotide (NADH), flavin adenine dinucleotide (FAD), and reactive oxygen species (ROS).
Biomedical applications of TPM in neuroscience and cancer detection are demonstrated.
© 2013 Chinese Optics Letters
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