Abstract

The fluorescence power from biological tissue excited by a femtosecond laser pulse compared with excitation power does not appear to obey a simple quadratic relationship given by the steady non-linear theory. A more reliable analysis is developed based on transient two-photon absorption because the response time of two-photon absorption is longer than the width of a femtosecond pulse. Good agreement is obtained between the theoretical analysis and the experimental results of fluorescence power versus excitation power. This letter offers potential value to non-linear optics in biological tissues.

© 2012 Chinese Optics Letters

PDF Article

References

  • View by:
  • |
  • |
  • |

  1. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
  2. C. Xu and W. Zipfel, Proc. Anatl. Acad. Sci. 93, 10763 (1996).
  3. P. T. C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Riomed. 2, 399 (2000).
  4. V. E. Centonze and J. G. White, Biophys. J. 75, 2015 (1998).
  5. F. Helmchen and W. Denk, Nat. Methoods. 2, 932 (2005).
  6. W. R. Zipfel and R. M. Williams, Nature Biotechnol. 21, 1369 (2003).
  7. J. B. Dominguez and Y. Berube-Lauzire, Biomed. Opt. Express 2, 817 (2011).
  8. R. Cicchi and F. S. Pavone, Anal. Bioanal. Chem. 400, 2687 (2011).
  9. H. Quan and Z. Guo, Opt. Express 12, 449 (2004).
  10. Y. R. Shen, The Principles of Nonlinear Optics (John Wiley & Sons, New York, 1984).
  11. Z. Song and T. Nakajima, Chin. Opt. Lett. 9, 10101 (2011).
  12. R. L. Carman, F. Shimizu, C. S. Wang, and N. Bloembergen, Phys. Rev. A 2, 60 (1970).
  13. J.-K. Wang, Y. Siegal, C. Lu, and E. Mazur, J. Opt. Soc. Am B 11, 1031 (1994).

2011 (3)

R. Cicchi and F. S. Pavone, Anal. Bioanal. Chem. 400, 2687 (2011).

Z. Song and T. Nakajima, Chin. Opt. Lett. 9, 10101 (2011).

J. B. Dominguez and Y. Berube-Lauzire, Biomed. Opt. Express 2, 817 (2011).

2005 (1)

F. Helmchen and W. Denk, Nat. Methoods. 2, 932 (2005).

2004 (1)

2003 (1)

W. R. Zipfel and R. M. Williams, Nature Biotechnol. 21, 1369 (2003).

2000 (1)

P. T. C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Riomed. 2, 399 (2000).

1998 (1)

V. E. Centonze and J. G. White, Biophys. J. 75, 2015 (1998).

1996 (1)

C. Xu and W. Zipfel, Proc. Anatl. Acad. Sci. 93, 10763 (1996).

1994 (1)

J.-K. Wang, Y. Siegal, C. Lu, and E. Mazur, J. Opt. Soc. Am B 11, 1031 (1994).

1990 (1)

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).

1970 (1)

R. L. Carman, F. Shimizu, C. S. Wang, and N. Bloembergen, Phys. Rev. A 2, 60 (1970).

Berland, K. M.

P. T. C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Riomed. 2, 399 (2000).

Berube-Lauzire, Y.

Bloembergen, N.

R. L. Carman, F. Shimizu, C. S. Wang, and N. Bloembergen, Phys. Rev. A 2, 60 (1970).

Carman, R. L.

R. L. Carman, F. Shimizu, C. S. Wang, and N. Bloembergen, Phys. Rev. A 2, 60 (1970).

Centonze, V. E.

V. E. Centonze and J. G. White, Biophys. J. 75, 2015 (1998).

Cicchi, R.

R. Cicchi and F. S. Pavone, Anal. Bioanal. Chem. 400, 2687 (2011).

Denk, W.

F. Helmchen and W. Denk, Nat. Methoods. 2, 932 (2005).

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).

Dominguez, J. B.

Dong, P. T. C. Y.

P. T. C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Riomed. 2, 399 (2000).

Guo, Z.

Helmchen, F.

F. Helmchen and W. Denk, Nat. Methoods. 2, 932 (2005).

Lu, C.

J.-K. Wang, Y. Siegal, C. Lu, and E. Mazur, J. Opt. Soc. Am B 11, 1031 (1994).

Masters, B. R.

P. T. C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Riomed. 2, 399 (2000).

Mazur, E.

J.-K. Wang, Y. Siegal, C. Lu, and E. Mazur, J. Opt. Soc. Am B 11, 1031 (1994).

Nakajima, T.

Z. Song and T. Nakajima, Chin. Opt. Lett. 9, 10101 (2011).

Pavone, F. S.

R. Cicchi and F. S. Pavone, Anal. Bioanal. Chem. 400, 2687 (2011).

Quan, H.

Shimizu, F.

R. L. Carman, F. Shimizu, C. S. Wang, and N. Bloembergen, Phys. Rev. A 2, 60 (1970).

Siegal, Y.

J.-K. Wang, Y. Siegal, C. Lu, and E. Mazur, J. Opt. Soc. Am B 11, 1031 (1994).

Song, Z.

Z. Song and T. Nakajima, Chin. Opt. Lett. 9, 10101 (2011).

Strickler, J. H.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).

Wang, C. S.

R. L. Carman, F. Shimizu, C. S. Wang, and N. Bloembergen, Phys. Rev. A 2, 60 (1970).

Wang, J.-K.

J.-K. Wang, Y. Siegal, C. Lu, and E. Mazur, J. Opt. Soc. Am B 11, 1031 (1994).

Webb, W. W.

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).

White, J. G.

V. E. Centonze and J. G. White, Biophys. J. 75, 2015 (1998).

Williams, R. M.

W. R. Zipfel and R. M. Williams, Nature Biotechnol. 21, 1369 (2003).

Xu, C.

C. Xu and W. Zipfel, Proc. Anatl. Acad. Sci. 93, 10763 (1996).

Zipfel, W.

C. Xu and W. Zipfel, Proc. Anatl. Acad. Sci. 93, 10763 (1996).

Zipfel, W. R.

W. R. Zipfel and R. M. Williams, Nature Biotechnol. 21, 1369 (2003).

Anal. Bioanal. Chem. (1)

R. Cicchi and F. S. Pavone, Anal. Bioanal. Chem. 400, 2687 (2011).

Annu. Riomed. (1)

P. T. C. Y. Dong, B. R. Masters, and K. M. Berland, Annu. Riomed. 2, 399 (2000).

Biomed. Opt. Express (1)

Biophys. J. (1)

V. E. Centonze and J. G. White, Biophys. J. 75, 2015 (1998).

Chin. Opt. Lett. (1)

Z. Song and T. Nakajima, Chin. Opt. Lett. 9, 10101 (2011).

J. Opt. Soc. Am B (1)

J.-K. Wang, Y. Siegal, C. Lu, and E. Mazur, J. Opt. Soc. Am B 11, 1031 (1994).

Nat. Methoods. (1)

F. Helmchen and W. Denk, Nat. Methoods. 2, 932 (2005).

Nature Biotechnol. (1)

W. R. Zipfel and R. M. Williams, Nature Biotechnol. 21, 1369 (2003).

Opt. Express (1)

Phys. Rev. A (1)

R. L. Carman, F. Shimizu, C. S. Wang, and N. Bloembergen, Phys. Rev. A 2, 60 (1970).

Proc. Anatl. Acad. Sci. (1)

C. Xu and W. Zipfel, Proc. Anatl. Acad. Sci. 93, 10763 (1996).

Science (1)

W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).

Other (1)

Y. R. Shen, The Principles of Nonlinear Optics (John Wiley & Sons, New York, 1984).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.