Abstract

Since RGD peptides (R: arginine; G: glycine; D: aspartic acid) are found to promote cell adhesion, they are modified at numerous materials surface for medical applications such as drug delivery and regenerative medicine. Peptide-cell surface interactions play a key role in the above applications. In this letter, we study the adhesion force between the RGD-coated bead and Hela cell surface by optical tweezes. The adhesion is dominated by the binding of α<sub>5</sub>β<sub>1</sub> and RGD-peptide with higher adhesion probability and stronger adhesion strength compared with the adhesion of bare bead and cell surface. The binding force for a single α<sub>5</sub>β<sub>1</sub>-GRGDSP pair is determined to be 16.8 pN at a loading rate of 1.5 nN/s. The unstressed off-rate is 1.65 × 10<sup>-2</sup> s<sup>-1</sup> and the distance of transition state for the rigid binding model is 3.0 nm.

© 2012 Chinese Optics Letters

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2011 (2)

2008 (2)

T. Erdmann, S. Pierrat, P. Nassoy, and U. S. Schwarz, Europhys. Lett. 81, 48001 (2008).

K. C. Neuman and A. Nagy, Nat. Methods 5, 491 (2008).

2007 (1)

E. A. Evans and D. A. Calderwood, Science 316, 1148 (2007).

2004 (1)

M. Backer, T. Gaynutdinov, V. Patel, B. Jehning, E. Myshkin, and J. Backer, Bioconjugate Chem. 15, 1021 (2004).

2000 (1)

D. Leckband, Annu. Rev. Biophys. Biomol. Struct. 29, 1 (2000).

1999 (2)

P. Lehenkari and M. Horton, Biochem. Biophys. Res. Commum. 259, 645 (1999).

V. Serru, F. Le Naour, M. Billard, D. Azorsa, F. Lanza, C. Boucheix, and E. Rubinstein, Biochem. J. 340, 103 (1999).

1997 (1)

E. Evans and K. Ritchie, Biophys. J. 72, 1541 (1997).

1986 (1)

1984 (1)

M. D. Pierschbacher and E. Ruoslahti, Nature 309, 30 (1984).

1978 (1)

G. Bell, Science 200, 618 (1978).

Ashkin, A.

Azorsa, D.

V. Serru, F. Le Naour, M. Billard, D. Azorsa, F. Lanza, C. Boucheix, and E. Rubinstein, Biochem. J. 340, 103 (1999).

Backer, J.

M. Backer, T. Gaynutdinov, V. Patel, B. Jehning, E. Myshkin, and J. Backer, Bioconjugate Chem. 15, 1021 (2004).

Backer, M.

M. Backer, T. Gaynutdinov, V. Patel, B. Jehning, E. Myshkin, and J. Backer, Bioconjugate Chem. 15, 1021 (2004).

Bell, G.

G. Bell, Science 200, 618 (1978).

Billard, M.

V. Serru, F. Le Naour, M. Billard, D. Azorsa, F. Lanza, C. Boucheix, and E. Rubinstein, Biochem. J. 340, 103 (1999).

Bjorkholm, J. E.

Boucheix, C.

V. Serru, F. Le Naour, M. Billard, D. Azorsa, F. Lanza, C. Boucheix, and E. Rubinstein, Biochem. J. 340, 103 (1999).

Bu, J.

Calderwood, D. A.

E. A. Evans and D. A. Calderwood, Science 316, 1148 (2007).

Cao, R.

Chu, S.

Dziedzic, J. M.

Erdmann, T.

T. Erdmann, S. Pierrat, P. Nassoy, and U. S. Schwarz, Europhys. Lett. 81, 48001 (2008).

Evans, E.

E. Evans and K. Ritchie, Biophys. J. 72, 1541 (1997).

Evans, E. A.

E. A. Evans and D. A. Calderwood, Science 316, 1148 (2007).

Gaynutdinov, T.

M. Backer, T. Gaynutdinov, V. Patel, B. Jehning, E. Myshkin, and J. Backer, Bioconjugate Chem. 15, 1021 (2004).

Horton, M.

P. Lehenkari and M. Horton, Biochem. Biophys. Res. Commum. 259, 645 (1999).

Jehning, B.

M. Backer, T. Gaynutdinov, V. Patel, B. Jehning, E. Myshkin, and J. Backer, Bioconjugate Chem. 15, 1021 (2004).

Lanza, F.

V. Serru, F. Le Naour, M. Billard, D. Azorsa, F. Lanza, C. Boucheix, and E. Rubinstein, Biochem. J. 340, 103 (1999).

Leckband, D.

D. Leckband, Annu. Rev. Biophys. Biomol. Struct. 29, 1 (2000).

Lehenkari, P.

P. Lehenkari and M. Horton, Biochem. Biophys. Res. Commum. 259, 645 (1999).

Myshkin, E.

M. Backer, T. Gaynutdinov, V. Patel, B. Jehning, E. Myshkin, and J. Backer, Bioconjugate Chem. 15, 1021 (2004).

Nagy, A.

K. C. Neuman and A. Nagy, Nat. Methods 5, 491 (2008).

Naour, F. Le

V. Serru, F. Le Naour, M. Billard, D. Azorsa, F. Lanza, C. Boucheix, and E. Rubinstein, Biochem. J. 340, 103 (1999).

Nassoy, P.

T. Erdmann, S. Pierrat, P. Nassoy, and U. S. Schwarz, Europhys. Lett. 81, 48001 (2008).

Neuman, K. C.

K. C. Neuman and A. Nagy, Nat. Methods 5, 491 (2008).

Patel, V.

M. Backer, T. Gaynutdinov, V. Patel, B. Jehning, E. Myshkin, and J. Backer, Bioconjugate Chem. 15, 1021 (2004).

Pierrat, S.

T. Erdmann, S. Pierrat, P. Nassoy, and U. S. Schwarz, Europhys. Lett. 81, 48001 (2008).

Pierschbacher, M. D.

M. D. Pierschbacher and E. Ruoslahti, Nature 309, 30 (1984).

Ritchie, K.

E. Evans and K. Ritchie, Biophys. J. 72, 1541 (1997).

Rubinstein, E.

V. Serru, F. Le Naour, M. Billard, D. Azorsa, F. Lanza, C. Boucheix, and E. Rubinstein, Biochem. J. 340, 103 (1999).

Ruoslahti, E.

M. D. Pierschbacher and E. Ruoslahti, Nature 309, 30 (1984).

Schwarz, U. S.

T. Erdmann, S. Pierrat, P. Nassoy, and U. S. Schwarz, Europhys. Lett. 81, 48001 (2008).

Serru, V.

V. Serru, F. Le Naour, M. Billard, D. Azorsa, F. Lanza, C. Boucheix, and E. Rubinstein, Biochem. J. 340, 103 (1999).

Sun, Y.

Yuan, G.

Yuan, X.

Zhang, Y.

Zhao, X.

Zhu, S.

Annu. Rev. Biophys. Biomol. Struct. (1)

D. Leckband, Annu. Rev. Biophys. Biomol. Struct. 29, 1 (2000).

Biochem. Biophys. Res. Commum. (1)

P. Lehenkari and M. Horton, Biochem. Biophys. Res. Commum. 259, 645 (1999).

Biochem. J. (1)

V. Serru, F. Le Naour, M. Billard, D. Azorsa, F. Lanza, C. Boucheix, and E. Rubinstein, Biochem. J. 340, 103 (1999).

Bioconjugate Chem. (1)

M. Backer, T. Gaynutdinov, V. Patel, B. Jehning, E. Myshkin, and J. Backer, Bioconjugate Chem. 15, 1021 (2004).

Biophys. J. (1)

E. Evans and K. Ritchie, Biophys. J. 72, 1541 (1997).

Chin. Opt. Lett. (2)

Europhys. Lett. (1)

T. Erdmann, S. Pierrat, P. Nassoy, and U. S. Schwarz, Europhys. Lett. 81, 48001 (2008).

Nat. Methods (1)

K. C. Neuman and A. Nagy, Nat. Methods 5, 491 (2008).

Nature (1)

M. D. Pierschbacher and E. Ruoslahti, Nature 309, 30 (1984).

Opt. Lett. (1)

Science (2)

E. A. Evans and D. A. Calderwood, Science 316, 1148 (2007).

G. Bell, Science 200, 618 (1978).

Other (1)

J. Happel and H. Brenner, Low Reynolds Number Hydrodynamics (Martinus Nijhoff, Hague, 1983).

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