Abstract

Protein microarray technology has recently emerged as a powerful tool for biomedical research. Before automatic analysis the protein microarray images, protein spots in the images must be determined appropriately by spot segmentation algorithm. In this paper, an improved seeded region growing (ISRG) algorithm for protein microarray segmentation is presented, the seeds are obtained by finding the positions of the printed spots, and the protein spot regions are grown through these seeds. The experiment results show that the presented algorithm is accurate for adaptive shape segmentation and robust for proteinmicroarray images contaminated by noise.

© 2005 Chinese Optics Letters

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  1. J. H. Ng and L. L. Ilag, J. Cellular and Molecular Medicine 6, 329 (2002).
  2. Y. H. Yang, M. J. Buckley, S. Dudoit, and T. P. Speed, J. Computational and Graphical Statistics 11, 108 (2002).
  3. A. W.-C. Liew, H. Yan, and M. Yang, Pattern Recognition 36, 1251 (2003).
  4. R. Hirata Jr., J. Barrera, R. F. Hashimoto, D. O. Dantas, and G. H. Esteves, Real-Time Imaging 8, 491 (2002).
  5. Y. Chen, E. R. Dougherty, and M. L. Bittner, J. Biomedical Optics 2, 364 (1997).
  6. A. Mehnert and P. Jackway, Pattern Recognition Lett. 18, 1065 (1997).
  7. R. Adams and L. Bischof, IEEE Trans. Pattern Analysis and Machine Intelligence 16, 641 (1994).
  8. J. Quackenbush, Nature Reviews Genetics 2, 418 (2001).

2003 (1)

A. W.-C. Liew, H. Yan, and M. Yang, Pattern Recognition 36, 1251 (2003).

2002 (3)

R. Hirata Jr., J. Barrera, R. F. Hashimoto, D. O. Dantas, and G. H. Esteves, Real-Time Imaging 8, 491 (2002).

J. H. Ng and L. L. Ilag, J. Cellular and Molecular Medicine 6, 329 (2002).

Y. H. Yang, M. J. Buckley, S. Dudoit, and T. P. Speed, J. Computational and Graphical Statistics 11, 108 (2002).

2001 (1)

J. Quackenbush, Nature Reviews Genetics 2, 418 (2001).

1997 (2)

Y. Chen, E. R. Dougherty, and M. L. Bittner, J. Biomedical Optics 2, 364 (1997).

A. Mehnert and P. Jackway, Pattern Recognition Lett. 18, 1065 (1997).

1994 (1)

R. Adams and L. Bischof, IEEE Trans. Pattern Analysis and Machine Intelligence 16, 641 (1994).

Adams, R.

R. Adams and L. Bischof, IEEE Trans. Pattern Analysis and Machine Intelligence 16, 641 (1994).

Barrera, J.

R. Hirata Jr., J. Barrera, R. F. Hashimoto, D. O. Dantas, and G. H. Esteves, Real-Time Imaging 8, 491 (2002).

Bischof, L.

R. Adams and L. Bischof, IEEE Trans. Pattern Analysis and Machine Intelligence 16, 641 (1994).

Bittner, M. L.

Y. Chen, E. R. Dougherty, and M. L. Bittner, J. Biomedical Optics 2, 364 (1997).

Buckley, M. J.

Y. H. Yang, M. J. Buckley, S. Dudoit, and T. P. Speed, J. Computational and Graphical Statistics 11, 108 (2002).

Chen, Y.

Y. Chen, E. R. Dougherty, and M. L. Bittner, J. Biomedical Optics 2, 364 (1997).

Dantas, D. O.

R. Hirata Jr., J. Barrera, R. F. Hashimoto, D. O. Dantas, and G. H. Esteves, Real-Time Imaging 8, 491 (2002).

Dougherty, E. R.

Y. Chen, E. R. Dougherty, and M. L. Bittner, J. Biomedical Optics 2, 364 (1997).

Dudoit, S.

Y. H. Yang, M. J. Buckley, S. Dudoit, and T. P. Speed, J. Computational and Graphical Statistics 11, 108 (2002).

Esteves, G. H.

R. Hirata Jr., J. Barrera, R. F. Hashimoto, D. O. Dantas, and G. H. Esteves, Real-Time Imaging 8, 491 (2002).

Hashimoto, R. F.

R. Hirata Jr., J. Barrera, R. F. Hashimoto, D. O. Dantas, and G. H. Esteves, Real-Time Imaging 8, 491 (2002).

Ilag, L. L.

J. H. Ng and L. L. Ilag, J. Cellular and Molecular Medicine 6, 329 (2002).

Jackway, P.

A. Mehnert and P. Jackway, Pattern Recognition Lett. 18, 1065 (1997).

Jr., R. Hirata

R. Hirata Jr., J. Barrera, R. F. Hashimoto, D. O. Dantas, and G. H. Esteves, Real-Time Imaging 8, 491 (2002).

Liew, A. W.-C.

A. W.-C. Liew, H. Yan, and M. Yang, Pattern Recognition 36, 1251 (2003).

Mehnert, A.

A. Mehnert and P. Jackway, Pattern Recognition Lett. 18, 1065 (1997).

Ng, J. H.

J. H. Ng and L. L. Ilag, J. Cellular and Molecular Medicine 6, 329 (2002).

Quackenbush, J.

J. Quackenbush, Nature Reviews Genetics 2, 418 (2001).

Speed, T. P.

Y. H. Yang, M. J. Buckley, S. Dudoit, and T. P. Speed, J. Computational and Graphical Statistics 11, 108 (2002).

Yan, H.

A. W.-C. Liew, H. Yan, and M. Yang, Pattern Recognition 36, 1251 (2003).

Yang, M.

A. W.-C. Liew, H. Yan, and M. Yang, Pattern Recognition 36, 1251 (2003).

Yang, Y. H.

Y. H. Yang, M. J. Buckley, S. Dudoit, and T. P. Speed, J. Computational and Graphical Statistics 11, 108 (2002).

IEEE Trans. Pattern Analysis and Machine Intelligence (1)

R. Adams and L. Bischof, IEEE Trans. Pattern Analysis and Machine Intelligence 16, 641 (1994).

J. Biomedical Optics (1)

Y. Chen, E. R. Dougherty, and M. L. Bittner, J. Biomedical Optics 2, 364 (1997).

J. Cellular and Molecular Medicine (1)

J. H. Ng and L. L. Ilag, J. Cellular and Molecular Medicine 6, 329 (2002).

J. Computational and Graphical Statistics (1)

Y. H. Yang, M. J. Buckley, S. Dudoit, and T. P. Speed, J. Computational and Graphical Statistics 11, 108 (2002).

Nature Reviews Genetics (1)

J. Quackenbush, Nature Reviews Genetics 2, 418 (2001).

Pattern Recognition (1)

A. W.-C. Liew, H. Yan, and M. Yang, Pattern Recognition 36, 1251 (2003).

Pattern Recognition Lett. (1)

A. Mehnert and P. Jackway, Pattern Recognition Lett. 18, 1065 (1997).

Real-Time Imaging (1)

R. Hirata Jr., J. Barrera, R. F. Hashimoto, D. O. Dantas, and G. H. Esteves, Real-Time Imaging 8, 491 (2002).

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