Abstract

Employing nonlinear spectral imaging technique based on two-photon-excited fluorescence and second-harmonic generation (SHG) of biological tissue, we combine the image-guided spectral analysis method and multi-channel subsequent detection imaging to map and visualize the intrinsic species in a native rabbit aortic wall. A series of recorded nonlinear spectral images excited by a broad range of laser wavelengths (730-910 nm) are used to identify five components in the native rabbit aortic wall, including nicotinamide adenine dinucleotide (NADH), elastic fiber, flavin, porphyrin derivatives, and collagen. Integrating multi-channel subsequent detection imaging technique, the high-resolution, high contrast images of collagen and elastic fiber in the aortic wall are obtained. Our results demonstrate that this method can yield complementary biochemical and morphological information about aortic tissues, which have the potential to determine the tissue pathology associated with mechanical properties of aortic wall and to evaluate the pharmacodynamical studies of vessels.

© 2009 Chinese Optics Letters

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  1. G. Boyer and K. Plamann, Chin. Opt. Lett. 5, 477 (2007).
  2. S. Zhuo, J. Chen, T. Luo, D. Zou, and J. Zhao, Opt. Express 14, 7810 (2006).
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  7. S. Zhuo, J. Chen, X. Jiang, K. Lu, and S. Xie, Laser Phys. Lett. 5, 614 (2008).
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  10. J. Chen, S. Zhuo, T. Luo, X. Jiang, and J. Zhao, Scanning 28, 319 (2006).
  11. J. Chen, S. Zhuo, R. Chen, X. Jiang, S. Xie, and Q. Zou, New J. Phys. 9, 212 (2007).
  12. S. Zhuo, J. Chen, X. Jiang, X. Cheng, and S. Xie, Skin Res. Technol. 13, 406 (2007).
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2008 (4)

S. Zhuo, J. Chen, B. Yu, X. Jiang, T. Luo, Q. Liu, R. Chen, and S. Xie, J. Biomed. Opt. 13, 054024 (2008).

S. Zhuo, J. Chen, N. Cao, X. Jiang, S. Xie, and S. Xiong, Phys. Med. Biol. 53, 3317 (2008).

S. Zhuo, J. Chen, X. Jiang, K. Lu, and S. Xie, Laser Phys. Lett. 5, 614 (2008).

J. Chen, S. Zhuo, T. Luo, D. Liu, and J. Zhao, Optik 119, 519 (2008).

2007 (5)

S. Zhuo, J. Chen, X. Jiang, T. Luo, R. Chen, S. Xie, and Q. Zou, Scanning 29, 219 (2007).

G. Boyer and K. Plamann, Chin. Opt. Lett. 5, 477 (2007).

S. Zhuo, J. Chen, X. Jiang, S. Xie, R. Chen, N. Cao, Q. Zou, and S. Xiong, Phys. Med. Biol. 52, 4967 (2007).

J. Chen, S. Zhuo, R. Chen, X. Jiang, S. Xie, and Q. Zou, New J. Phys. 9, 212 (2007).

S. Zhuo, J. Chen, X. Jiang, X. Cheng, and S. Xie, Skin Res. Technol. 13, 406 (2007).

2006 (4)

2005 (1)

T. Boulesteix, A.-M. Pena, N. Pagès, G. Godeau, M.-P. Sauviat, E. Beaurepaire, and M.-C. Schanne-Klein, Cytometry A 69, 20 (2005).

2003 (1)

P. J. Campagnola and L. M. Loew, Nature Biotechnol. 21, 1356 (2003).

1996 (1)

R. Richards-Kortum and E. Sevick-Muraca, Annu. Rev. Phys. Chem. 47, 555 (1996).

Beaurepaire, E.

T. Boulesteix, A.-M. Pena, N. Pagès, G. Godeau, M.-P. Sauviat, E. Beaurepaire, and M.-C. Schanne-Klein, Cytometry A 69, 20 (2005).

Boulesteix, T.

T. Boulesteix, A.-M. Pena, N. Pagès, G. Godeau, M.-P. Sauviat, E. Beaurepaire, and M.-C. Schanne-Klein, Cytometry A 69, 20 (2005).

Boyer, G.

Bruijn, H. S. de

Campagnola, P. J.

P. J. Campagnola and L. M. Loew, Nature Biotechnol. 21, 1356 (2003).

Cao, N.

S. Zhuo, J. Chen, N. Cao, X. Jiang, S. Xie, and S. Xiong, Phys. Med. Biol. 53, 3317 (2008).

S. Zhuo, J. Chen, X. Jiang, S. Xie, R. Chen, N. Cao, Q. Zou, and S. Xiong, Phys. Med. Biol. 52, 4967 (2007).

Chen, J.

S. Zhuo, J. Chen, N. Cao, X. Jiang, S. Xie, and S. Xiong, Phys. Med. Biol. 53, 3317 (2008).

S. Zhuo, J. Chen, B. Yu, X. Jiang, T. Luo, Q. Liu, R. Chen, and S. Xie, J. Biomed. Opt. 13, 054024 (2008).

J. Chen, S. Zhuo, T. Luo, D. Liu, and J. Zhao, Optik 119, 519 (2008).

S. Zhuo, J. Chen, X. Jiang, K. Lu, and S. Xie, Laser Phys. Lett. 5, 614 (2008).

J. Chen, S. Zhuo, R. Chen, X. Jiang, S. Xie, and Q. Zou, New J. Phys. 9, 212 (2007).

S. Zhuo, J. Chen, X. Jiang, T. Luo, R. Chen, S. Xie, and Q. Zou, Scanning 29, 219 (2007).

S. Zhuo, J. Chen, X. Jiang, S. Xie, R. Chen, N. Cao, Q. Zou, and S. Xiong, Phys. Med. Biol. 52, 4967 (2007).

S. Zhuo, J. Chen, X. Jiang, X. Cheng, and S. Xie, Skin Res. Technol. 13, 406 (2007).

J. Chen, S. Zhuo, T. Luo, and J. Zhao, Chin. Opt. Lett. 4, 598 (2006).

S. Zhuo, J. Chen, T. Luo, D. Zou, and J. Zhao, Opt. Express 14, 7810 (2006).

J. Chen, S. Zhuo, T. Luo, X. Jiang, and J. Zhao, Scanning 28, 319 (2006).

Chen, R.

S. Zhuo, J. Chen, B. Yu, X. Jiang, T. Luo, Q. Liu, R. Chen, and S. Xie, J. Biomed. Opt. 13, 054024 (2008).

J. Chen, S. Zhuo, R. Chen, X. Jiang, S. Xie, and Q. Zou, New J. Phys. 9, 212 (2007).

S. Zhuo, J. Chen, X. Jiang, T. Luo, R. Chen, S. Xie, and Q. Zou, Scanning 29, 219 (2007).

S. Zhuo, J. Chen, X. Jiang, S. Xie, R. Chen, N. Cao, Q. Zou, and S. Xiong, Phys. Med. Biol. 52, 4967 (2007).

Cheng, X.

S. Zhuo, J. Chen, X. Jiang, X. Cheng, and S. Xie, Skin Res. Technol. 13, 406 (2007).

Gerritsen, H. C.

Godeau, G.

T. Boulesteix, A.-M. Pena, N. Pagès, G. Godeau, M.-P. Sauviat, E. Beaurepaire, and M.-C. Schanne-Klein, Cytometry A 69, 20 (2005).

Heuvel, A. van der Ploeg-van den

Jiang, X.

S. Zhuo, J. Chen, N. Cao, X. Jiang, S. Xie, and S. Xiong, Phys. Med. Biol. 53, 3317 (2008).

S. Zhuo, J. Chen, X. Jiang, K. Lu, and S. Xie, Laser Phys. Lett. 5, 614 (2008).

S. Zhuo, J. Chen, B. Yu, X. Jiang, T. Luo, Q. Liu, R. Chen, and S. Xie, J. Biomed. Opt. 13, 054024 (2008).

J. Chen, S. Zhuo, R. Chen, X. Jiang, S. Xie, and Q. Zou, New J. Phys. 9, 212 (2007).

S. Zhuo, J. Chen, X. Jiang, T. Luo, R. Chen, S. Xie, and Q. Zou, Scanning 29, 219 (2007).

S. Zhuo, J. Chen, X. Jiang, X. Cheng, and S. Xie, Skin Res. Technol. 13, 406 (2007).

S. Zhuo, J. Chen, X. Jiang, S. Xie, R. Chen, N. Cao, Q. Zou, and S. Xiong, Phys. Med. Biol. 52, 4967 (2007).

J. Chen, S. Zhuo, T. Luo, X. Jiang, and J. Zhao, Scanning 28, 319 (2006).

Liu, D.

J. Chen, S. Zhuo, T. Luo, D. Liu, and J. Zhao, Optik 119, 519 (2008).

Liu, Q.

S. Zhuo, J. Chen, B. Yu, X. Jiang, T. Luo, Q. Liu, R. Chen, and S. Xie, J. Biomed. Opt. 13, 054024 (2008).

Loew, L. M.

P. J. Campagnola and L. M. Loew, Nature Biotechnol. 21, 1356 (2003).

Lu, K.

S. Zhuo, J. Chen, X. Jiang, K. Lu, and S. Xie, Laser Phys. Lett. 5, 614 (2008).

Luo, T.

J. Chen, S. Zhuo, T. Luo, D. Liu, and J. Zhao, Optik 119, 519 (2008).

S. Zhuo, J. Chen, B. Yu, X. Jiang, T. Luo, Q. Liu, R. Chen, and S. Xie, J. Biomed. Opt. 13, 054024 (2008).

S. Zhuo, J. Chen, X. Jiang, T. Luo, R. Chen, S. Xie, and Q. Zou, Scanning 29, 219 (2007).

S. Zhuo, J. Chen, T. Luo, D. Zou, and J. Zhao, Opt. Express 14, 7810 (2006).

J. Chen, S. Zhuo, T. Luo, and J. Zhao, Chin. Opt. Lett. 4, 598 (2006).

J. Chen, S. Zhuo, T. Luo, X. Jiang, and J. Zhao, Scanning 28, 319 (2006).

Pagès, N.

T. Boulesteix, A.-M. Pena, N. Pagès, G. Godeau, M.-P. Sauviat, E. Beaurepaire, and M.-C. Schanne-Klein, Cytometry A 69, 20 (2005).

Palero, J. A.

Pena, A.-M.

T. Boulesteix, A.-M. Pena, N. Pagès, G. Godeau, M.-P. Sauviat, E. Beaurepaire, and M.-C. Schanne-Klein, Cytometry A 69, 20 (2005).

Plamann, K.

Richards-Kortum, R.

R. Richards-Kortum and E. Sevick-Muraca, Annu. Rev. Phys. Chem. 47, 555 (1996).

Sauviat, M.-P.

T. Boulesteix, A.-M. Pena, N. Pagès, G. Godeau, M.-P. Sauviat, E. Beaurepaire, and M.-C. Schanne-Klein, Cytometry A 69, 20 (2005).

Schanne-Klein, M.-C.

T. Boulesteix, A.-M. Pena, N. Pagès, G. Godeau, M.-P. Sauviat, E. Beaurepaire, and M.-C. Schanne-Klein, Cytometry A 69, 20 (2005).

Sevick-Muraca, E.

R. Richards-Kortum and E. Sevick-Muraca, Annu. Rev. Phys. Chem. 47, 555 (1996).

Sterenborg, H. J. C. M.

Xie, S.

S. Zhuo, J. Chen, N. Cao, X. Jiang, S. Xie, and S. Xiong, Phys. Med. Biol. 53, 3317 (2008).

S. Zhuo, J. Chen, B. Yu, X. Jiang, T. Luo, Q. Liu, R. Chen, and S. Xie, J. Biomed. Opt. 13, 054024 (2008).

S. Zhuo, J. Chen, X. Jiang, K. Lu, and S. Xie, Laser Phys. Lett. 5, 614 (2008).

J. Chen, S. Zhuo, R. Chen, X. Jiang, S. Xie, and Q. Zou, New J. Phys. 9, 212 (2007).

S. Zhuo, J. Chen, X. Jiang, T. Luo, R. Chen, S. Xie, and Q. Zou, Scanning 29, 219 (2007).

S. Zhuo, J. Chen, X. Jiang, X. Cheng, and S. Xie, Skin Res. Technol. 13, 406 (2007).

S. Zhuo, J. Chen, X. Jiang, S. Xie, R. Chen, N. Cao, Q. Zou, and S. Xiong, Phys. Med. Biol. 52, 4967 (2007).

Xiong, S.

S. Zhuo, J. Chen, N. Cao, X. Jiang, S. Xie, and S. Xiong, Phys. Med. Biol. 53, 3317 (2008).

S. Zhuo, J. Chen, X. Jiang, S. Xie, R. Chen, N. Cao, Q. Zou, and S. Xiong, Phys. Med. Biol. 52, 4967 (2007).

Yu, B.

S. Zhuo, J. Chen, B. Yu, X. Jiang, T. Luo, Q. Liu, R. Chen, and S. Xie, J. Biomed. Opt. 13, 054024 (2008).

Zhao, J.

J. Chen, S. Zhuo, T. Luo, D. Liu, and J. Zhao, Optik 119, 519 (2008).

J. Chen, S. Zhuo, T. Luo, X. Jiang, and J. Zhao, Scanning 28, 319 (2006).

J. Chen, S. Zhuo, T. Luo, and J. Zhao, Chin. Opt. Lett. 4, 598 (2006).

S. Zhuo, J. Chen, T. Luo, D. Zou, and J. Zhao, Opt. Express 14, 7810 (2006).

Zhuo, S.

S. Zhuo, J. Chen, N. Cao, X. Jiang, S. Xie, and S. Xiong, Phys. Med. Biol. 53, 3317 (2008).

S. Zhuo, J. Chen, X. Jiang, K. Lu, and S. Xie, Laser Phys. Lett. 5, 614 (2008).

J. Chen, S. Zhuo, T. Luo, D. Liu, and J. Zhao, Optik 119, 519 (2008).

S. Zhuo, J. Chen, B. Yu, X. Jiang, T. Luo, Q. Liu, R. Chen, and S. Xie, J. Biomed. Opt. 13, 054024 (2008).

J. Chen, S. Zhuo, R. Chen, X. Jiang, S. Xie, and Q. Zou, New J. Phys. 9, 212 (2007).

S. Zhuo, J. Chen, X. Jiang, T. Luo, R. Chen, S. Xie, and Q. Zou, Scanning 29, 219 (2007).

S. Zhuo, J. Chen, X. Jiang, X. Cheng, and S. Xie, Skin Res. Technol. 13, 406 (2007).

S. Zhuo, J. Chen, X. Jiang, S. Xie, R. Chen, N. Cao, Q. Zou, and S. Xiong, Phys. Med. Biol. 52, 4967 (2007).

J. Chen, S. Zhuo, T. Luo, and J. Zhao, Chin. Opt. Lett. 4, 598 (2006).

S. Zhuo, J. Chen, T. Luo, D. Zou, and J. Zhao, Opt. Express 14, 7810 (2006).

J. Chen, S. Zhuo, T. Luo, X. Jiang, and J. Zhao, Scanning 28, 319 (2006).

Zou, D.

Zou, Q.

S. Zhuo, J. Chen, X. Jiang, T. Luo, R. Chen, S. Xie, and Q. Zou, Scanning 29, 219 (2007).

S. Zhuo, J. Chen, X. Jiang, S. Xie, R. Chen, N. Cao, Q. Zou, and S. Xiong, Phys. Med. Biol. 52, 4967 (2007).

J. Chen, S. Zhuo, R. Chen, X. Jiang, S. Xie, and Q. Zou, New J. Phys. 9, 212 (2007).

Annu. Rev. Phys. Chem. (1)

R. Richards-Kortum and E. Sevick-Muraca, Annu. Rev. Phys. Chem. 47, 555 (1996).

Chin. Opt. Lett. (2)

Cytometry A (1)

T. Boulesteix, A.-M. Pena, N. Pagès, G. Godeau, M.-P. Sauviat, E. Beaurepaire, and M.-C. Schanne-Klein, Cytometry A 69, 20 (2005).

J. Biomed. Opt. (1)

S. Zhuo, J. Chen, B. Yu, X. Jiang, T. Luo, Q. Liu, R. Chen, and S. Xie, J. Biomed. Opt. 13, 054024 (2008).

Laser Phys. Lett. (1)

S. Zhuo, J. Chen, X. Jiang, K. Lu, and S. Xie, Laser Phys. Lett. 5, 614 (2008).

Nature Biotechnol. (1)

P. J. Campagnola and L. M. Loew, Nature Biotechnol. 21, 1356 (2003).

New J. Phys. (1)

J. Chen, S. Zhuo, R. Chen, X. Jiang, S. Xie, and Q. Zou, New J. Phys. 9, 212 (2007).

Opt. Express (2)

Optik (1)

J. Chen, S. Zhuo, T. Luo, D. Liu, and J. Zhao, Optik 119, 519 (2008).

Phys. Med. Biol. (2)

S. Zhuo, J. Chen, X. Jiang, S. Xie, R. Chen, N. Cao, Q. Zou, and S. Xiong, Phys. Med. Biol. 52, 4967 (2007).

S. Zhuo, J. Chen, N. Cao, X. Jiang, S. Xie, and S. Xiong, Phys. Med. Biol. 53, 3317 (2008).

Scanning (2)

J. Chen, S. Zhuo, T. Luo, X. Jiang, and J. Zhao, Scanning 28, 319 (2006).

S. Zhuo, J. Chen, X. Jiang, T. Luo, R. Chen, S. Xie, and Q. Zou, Scanning 29, 219 (2007).

Skin Res. Technol. (1)

S. Zhuo, J. Chen, X. Jiang, X. Cheng, and S. Xie, Skin Res. Technol. 13, 406 (2007).

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