Abstract

Cross-talk phenomenon in dual-labeled fluorescent microarray scanning is analyzed from cross-excitation and cross-emission. It is turned out that the spectral overlap of the fluorophores is crucial for cross-talk error, and this error can be corrected by an image subtraction method. The experiment was successfully applied to separate the Cy3 channel and the Cy5 channel in microarray scanning. The cross-talk error was reduced from more than 1% to about 0.1%.

© 2005 Chinese Optics Letters

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  1. N. Dhiman, R. bonilla, D. J. O'Kane, and G. A. Poland, Vaccine 20 (1-2), 22 (2002).
  2. G. MacBeath and S. L. Schreiber, Science 289 1760 (2000).
  3. J. Montagu and P. Honkanen, Proc. SPIE 3779, 284 (1999).
  4. K. Mossberg and M. Ericsson, J. Microsc. 158 (2), 215 (1990).
  5. A. Entwistle and M. Noble, J. Microsc. 168 (3), 219 (1992).
  6. L. Wang, Z. Lu, Y. Li, and X. Zheng, Acta Opt. Sin. 23 (suppl.), 829 (2003).
  7. K. Carlsson and K. Mossberg, J. Microsc. 167 (1), 23 (1992).
  8. H. Ahammer, T. T. J. DeVaney, M. Hartbauer, and H. A. Tritthart, Micron 30, 309 (1999).
  9. G. Hardiman, Microarrays Methods and Applications: Nuts & Bolts (DNA Press, Eagleville, 2003) pp. 100-114.

1999

J. Montagu and P. Honkanen, Proc. SPIE 3779, 284 (1999).

H. Ahammer, T. T. J. DeVaney, M. Hartbauer, and H. A. Tritthart, Micron 30, 309 (1999).

Ahammer, H.

H. Ahammer, T. T. J. DeVaney, M. Hartbauer, and H. A. Tritthart, Micron 30, 309 (1999).

DeVaney, T. T. J.

H. Ahammer, T. T. J. DeVaney, M. Hartbauer, and H. A. Tritthart, Micron 30, 309 (1999).

Hartbauer, M.

H. Ahammer, T. T. J. DeVaney, M. Hartbauer, and H. A. Tritthart, Micron 30, 309 (1999).

Honkanen, P.

J. Montagu and P. Honkanen, Proc. SPIE 3779, 284 (1999).

Montagu, J.

J. Montagu and P. Honkanen, Proc. SPIE 3779, 284 (1999).

Tritthart, H. A.

H. Ahammer, T. T. J. DeVaney, M. Hartbauer, and H. A. Tritthart, Micron 30, 309 (1999).

Micron

H. Ahammer, T. T. J. DeVaney, M. Hartbauer, and H. A. Tritthart, Micron 30, 309 (1999).

Proc. SPIE

J. Montagu and P. Honkanen, Proc. SPIE 3779, 284 (1999).

Other

K. Mossberg and M. Ericsson, J. Microsc. 158 (2), 215 (1990).

A. Entwistle and M. Noble, J. Microsc. 168 (3), 219 (1992).

L. Wang, Z. Lu, Y. Li, and X. Zheng, Acta Opt. Sin. 23 (suppl.), 829 (2003).

K. Carlsson and K. Mossberg, J. Microsc. 167 (1), 23 (1992).

G. Hardiman, Microarrays Methods and Applications: Nuts & Bolts (DNA Press, Eagleville, 2003) pp. 100-114.

N. Dhiman, R. bonilla, D. J. O'Kane, and G. A. Poland, Vaccine 20 (1-2), 22 (2002).

G. MacBeath and S. L. Schreiber, Science 289 1760 (2000).

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