Abstract

Photoacoustic microscopy (PAM) is a multiscale imaging technique. In optical-resolution photoacoustic microscopy (OR-PAM), a single mode (SM) fiber is normally used as the source of optical excitation to be focused into a diffraction-limited spot. Recent advances in OR-PAM have improved its imaging speed using microelectromechanical systems (MEMS). Here we report for the first time the use of a multimode (MM) fiber as the optical excitation source for high resolution OR-PAM in vivo imaging. A high-speed MEMS scanner based OR-PAM system combined with the mechanical movement to provide wide area imaging was used. The use of multimode fiber for achieving tight optical focus would make the optical alignment easier and high repetition rate light delivery possible for high-speed OR-PAM imaging. A lateral resolution of 3.5 µm and axial resolution of 27 µm with ~1.5 mm imaging depth was successfully demonstrated using the system. The efficacy of multimode fibers for achieving tight focus is beneficial for developing high-resolution photoacoustic endoscopy systems and can be combined with other optical endoscopic imaging modalities as well.

© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Full Article  |  PDF Article
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References

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2017 (5)

M. Moothanchery and M. Pramanik, “Performance Characterization of a Switchable Acoustic Resolution and Optical Resolution Photoacoustic Microscopy System,” Sensors (Basel) 17(2), 357 (2017).
[Crossref] [PubMed]

M. Moothanchery, R. Z. Seeni, C. Xu, and M. Pramanik, “In vivo studies of transdermal nanoparticle delivery with microneedles using photoacoustic microscopy,” Biomed. Opt. Express 8(12), 5483–5492 (2017).
[Crossref] [PubMed]

M. Moothanchery, A. Sharma, and M. Pramanik, “Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for in vivo small-animal blood vasculature imaging,” J. Vis. Exp. 124, e55810 (2017).
[PubMed]

S. Jeon, H. B. Song, J. Kim, B. J. Lee, R. Managuli, J. H. Kim, J. H. Kim, and C. Kim, “In Vivo Photoacoustic Imaging of Anterior Ocular Vasculature: A Random Sample Consensus Approach,” Sci. Rep. 7(1), 4318 (2017).
[Crossref] [PubMed]

K. Park, J. Y. Kim, C. Lee, S. Jeon, G. Lim, and C. Kim, “Handheld Photoacoustic Microscopy Probe,” Sci. Rep. 7(1), 13359 (2017).
[Crossref] [PubMed]

2016 (2)

L. V. Wang and J. Yao, “A practical guide to photoacoustic tomography in the life sciences,” Nat. Methods 13(8), 627–638 (2016).
[Crossref] [PubMed]

Y. Zhou, J. Yao, and L. V. Wang, “Tutorial on photoacoustic tomography,” J. Biomed. Opt. 21(6), 061007 (2016).
[Crossref] [PubMed]

2015 (3)

2014 (3)

J. Yao and L. V. Wang, “Photoacoustic Brain Imaging: from Microscopic to Macroscopic Scales,” Neurophotonics 1(1), 011003 (2014).
[Crossref] [PubMed]

S. Park, C. Lee, J. Kim, and C. Kim, “Acoustic resolution photoacoustic microscopy,” Biomed. Eng. Lett. 4(3), 213–222 (2014).
[Crossref]

W. Song, W. Zheng, R. Liu, R. Lin, H. Huang, X. Gong, S. Yang, R. Zhang, and L. Song, “Reflection-mode in vivo photoacoustic microscopy with subwavelength lateral resolution,” Biomed. Opt. Express 5(12), 4235–4241 (2014).
[Crossref] [PubMed]

2013 (2)

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett. 102(21), 211106 (2013).
[Crossref]

M. Hughes, T. P. Chang, and G.-Z. Yang, “Fiber bundle endocytoscopy,” Biomed. Opt. Express 4(12), 2781–2794 (2013).
[Crossref] [PubMed]

2012 (5)

J.-M. Yang, R. Chen, C. Favazza, J. Yao, C. Li, Z. Hu, Q. Zhou, K. K. Shung, and L. V. Wang, “A 2.5-mm diameter probe for photoacoustic and ultrasonic endoscopy,” Opt. Express 20(21), 23944–23953 (2012).
[Crossref] [PubMed]

J. Yao, C.-H. Huang, L. Wang, J.-M. Yang, L. Gao, K. I. Maslov, J. Zou, and L. V. Wang, “Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror,” J. Biomed. Opt. 17(8), 080505 (2012).
[Crossref] [PubMed]

L. V. Wang and S. Hu, “Photoacoustic Tomography: In Vivo Imaging from Organelles to Organs,” Science 335(6075), 1458–1462 (2012).
[Crossref] [PubMed]

T. Čižmár and K. Dholakia, “Exploiting multimode waveguides for pure fibre-based imaging,” Nat. Commun. 3, 1027 (2012).
[Crossref] [PubMed]

P. Shao, W. Shi, P. Hajireza, and R. J. Zemp, “Integrated micro-endoscopy system for simultaneous fluorescence and optical-resolution photoacoustic imaging,” J. Biomed. Opt. 17(7), 076024 (2012).
[Crossref] [PubMed]

2011 (2)

2010 (1)

2009 (2)

L. V. Wang, “Multiscale photoacoustic microscopy and computed tomography,” Nat. Photonics 3(9), 503–509 (2009).
[Crossref] [PubMed]

E. Z. Zhang, J. G. Laufer, R. B. Pedley, and P. C. Beard, “In vivo high-resolution 3D photoacoustic imaging of superficial vascular anatomy,” Phys. Med. Biol. 54(4), 1035–1046 (2009).
[Crossref] [PubMed]

2006 (1)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, “Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging,” Nat. Biotechnol. 24(7), 848–851 (2006).
[Crossref] [PubMed]

2005 (1)

Beard, P.

P. Beard, “Biomedical photoacoustic imaging,” Interface Focus 1(4), 602–631 (2011).
[Crossref] [PubMed]

Beard, P. C.

E. Z. Zhang, J. G. Laufer, R. B. Pedley, and P. C. Beard, “In vivo high-resolution 3D photoacoustic imaging of superficial vascular anatomy,” Phys. Med. Biol. 54(4), 1035–1046 (2009).
[Crossref] [PubMed]

Bossy, E.

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett. 102(21), 211106 (2013).
[Crossref]

Chang, T. P.

Chen, R.

Cižmár, T.

T. Čižmár and K. Dholakia, “Exploiting multimode waveguides for pure fibre-based imaging,” Nat. Commun. 3, 1027 (2012).
[Crossref] [PubMed]

Dholakia, K.

T. Čižmár and K. Dholakia, “Exploiting multimode waveguides for pure fibre-based imaging,” Nat. Commun. 3, 1027 (2012).
[Crossref] [PubMed]

Farahi, S.

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett. 102(21), 211106 (2013).
[Crossref]

Favazza, C.

Gao, L.

J. Yao, C.-H. Huang, L. Wang, J.-M. Yang, L. Gao, K. I. Maslov, J. Zou, and L. V. Wang, “Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror,” J. Biomed. Opt. 17(8), 080505 (2012).
[Crossref] [PubMed]

Gong, X.

Gu, R. Y.

Hajireza, P.

P. Shao, W. Shi, P. Hajireza, and R. J. Zemp, “Integrated micro-endoscopy system for simultaneous fluorescence and optical-resolution photoacoustic imaging,” J. Biomed. Opt. 17(7), 076024 (2012).
[Crossref] [PubMed]

Hu, S.

Hu, Z.

Huang, C.-H.

J. Yao, C.-H. Huang, L. Wang, J.-M. Yang, L. Gao, K. I. Maslov, J. Zou, and L. V. Wang, “Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror,” J. Biomed. Opt. 17(8), 080505 (2012).
[Crossref] [PubMed]

Huang, H.

Hughes, M.

Huignard, J. P.

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett. 102(21), 211106 (2013).
[Crossref]

Jeon, S.

S. Jeon, H. B. Song, J. Kim, B. J. Lee, R. Managuli, J. H. Kim, J. H. Kim, and C. Kim, “In Vivo Photoacoustic Imaging of Anterior Ocular Vasculature: A Random Sample Consensus Approach,” Sci. Rep. 7(1), 4318 (2017).
[Crossref] [PubMed]

K. Park, J. Y. Kim, C. Lee, S. Jeon, G. Lim, and C. Kim, “Handheld Photoacoustic Microscopy Probe,” Sci. Rep. 7(1), 13359 (2017).
[Crossref] [PubMed]

Kahn, J. M.

Kim, C.

K. Park, J. Y. Kim, C. Lee, S. Jeon, G. Lim, and C. Kim, “Handheld Photoacoustic Microscopy Probe,” Sci. Rep. 7(1), 13359 (2017).
[Crossref] [PubMed]

S. Jeon, H. B. Song, J. Kim, B. J. Lee, R. Managuli, J. H. Kim, J. H. Kim, and C. Kim, “In Vivo Photoacoustic Imaging of Anterior Ocular Vasculature: A Random Sample Consensus Approach,” Sci. Rep. 7(1), 4318 (2017).
[Crossref] [PubMed]

J. Y. Kim, C. Lee, K. Park, G. Lim, and C. Kim, “Fast optical-resolution photoacoustic microscopy using a 2-axis water-proofing MEMS scanner,” Sci. Rep. 5(1), 7932 (2015).
[Crossref] [PubMed]

S. Park, C. Lee, J. Kim, and C. Kim, “Acoustic resolution photoacoustic microscopy,” Biomed. Eng. Lett. 4(3), 213–222 (2014).
[Crossref]

Kim, J.

S. Jeon, H. B. Song, J. Kim, B. J. Lee, R. Managuli, J. H. Kim, J. H. Kim, and C. Kim, “In Vivo Photoacoustic Imaging of Anterior Ocular Vasculature: A Random Sample Consensus Approach,” Sci. Rep. 7(1), 4318 (2017).
[Crossref] [PubMed]

S. Park, C. Lee, J. Kim, and C. Kim, “Acoustic resolution photoacoustic microscopy,” Biomed. Eng. Lett. 4(3), 213–222 (2014).
[Crossref]

Kim, J. H.

S. Jeon, H. B. Song, J. Kim, B. J. Lee, R. Managuli, J. H. Kim, J. H. Kim, and C. Kim, “In Vivo Photoacoustic Imaging of Anterior Ocular Vasculature: A Random Sample Consensus Approach,” Sci. Rep. 7(1), 4318 (2017).
[Crossref] [PubMed]

S. Jeon, H. B. Song, J. Kim, B. J. Lee, R. Managuli, J. H. Kim, J. H. Kim, and C. Kim, “In Vivo Photoacoustic Imaging of Anterior Ocular Vasculature: A Random Sample Consensus Approach,” Sci. Rep. 7(1), 4318 (2017).
[Crossref] [PubMed]

Kim, J. Y.

K. Park, J. Y. Kim, C. Lee, S. Jeon, G. Lim, and C. Kim, “Handheld Photoacoustic Microscopy Probe,” Sci. Rep. 7(1), 13359 (2017).
[Crossref] [PubMed]

J. Y. Kim, C. Lee, K. Park, G. Lim, and C. Kim, “Fast optical-resolution photoacoustic microscopy using a 2-axis water-proofing MEMS scanner,” Sci. Rep. 5(1), 7932 (2015).
[Crossref] [PubMed]

Laufer, J. G.

E. Z. Zhang, J. G. Laufer, R. B. Pedley, and P. C. Beard, “In vivo high-resolution 3D photoacoustic imaging of superficial vascular anatomy,” Phys. Med. Biol. 54(4), 1035–1046 (2009).
[Crossref] [PubMed]

Lee, B. J.

S. Jeon, H. B. Song, J. Kim, B. J. Lee, R. Managuli, J. H. Kim, J. H. Kim, and C. Kim, “In Vivo Photoacoustic Imaging of Anterior Ocular Vasculature: A Random Sample Consensus Approach,” Sci. Rep. 7(1), 4318 (2017).
[Crossref] [PubMed]

Lee, C.

K. Park, J. Y. Kim, C. Lee, S. Jeon, G. Lim, and C. Kim, “Handheld Photoacoustic Microscopy Probe,” Sci. Rep. 7(1), 13359 (2017).
[Crossref] [PubMed]

J. Y. Kim, C. Lee, K. Park, G. Lim, and C. Kim, “Fast optical-resolution photoacoustic microscopy using a 2-axis water-proofing MEMS scanner,” Sci. Rep. 5(1), 7932 (2015).
[Crossref] [PubMed]

S. Park, C. Lee, J. Kim, and C. Kim, “Acoustic resolution photoacoustic microscopy,” Biomed. Eng. Lett. 4(3), 213–222 (2014).
[Crossref]

Li, C.

Lim, G.

K. Park, J. Y. Kim, C. Lee, S. Jeon, G. Lim, and C. Kim, “Handheld Photoacoustic Microscopy Probe,” Sci. Rep. 7(1), 13359 (2017).
[Crossref] [PubMed]

J. Y. Kim, C. Lee, K. Park, G. Lim, and C. Kim, “Fast optical-resolution photoacoustic microscopy using a 2-axis water-proofing MEMS scanner,” Sci. Rep. 5(1), 7932 (2015).
[Crossref] [PubMed]

Lin, R.

Liu, R.

Mahalati, R. N.

Managuli, R.

S. Jeon, H. B. Song, J. Kim, B. J. Lee, R. Managuli, J. H. Kim, J. H. Kim, and C. Kim, “In Vivo Photoacoustic Imaging of Anterior Ocular Vasculature: A Random Sample Consensus Approach,” Sci. Rep. 7(1), 4318 (2017).
[Crossref] [PubMed]

Maslov, K.

Maslov, K. I.

J. Yao, C.-H. Huang, L. Wang, J.-M. Yang, L. Gao, K. I. Maslov, J. Zou, and L. V. Wang, “Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror,” J. Biomed. Opt. 17(8), 080505 (2012).
[Crossref] [PubMed]

Moothanchery, M.

M. Moothanchery, A. Sharma, and M. Pramanik, “Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for in vivo small-animal blood vasculature imaging,” J. Vis. Exp. 124, e55810 (2017).
[PubMed]

M. Moothanchery and M. Pramanik, “Performance Characterization of a Switchable Acoustic Resolution and Optical Resolution Photoacoustic Microscopy System,” Sensors (Basel) 17(2), 357 (2017).
[Crossref] [PubMed]

M. Moothanchery, R. Z. Seeni, C. Xu, and M. Pramanik, “In vivo studies of transdermal nanoparticle delivery with microneedles using photoacoustic microscopy,” Biomed. Opt. Express 8(12), 5483–5492 (2017).
[Crossref] [PubMed]

Moser, C.

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett. 102(21), 211106 (2013).
[Crossref]

Papadopoulos, I. N.

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett. 102(21), 211106 (2013).
[Crossref]

Park, K.

K. Park, J. Y. Kim, C. Lee, S. Jeon, G. Lim, and C. Kim, “Handheld Photoacoustic Microscopy Probe,” Sci. Rep. 7(1), 13359 (2017).
[Crossref] [PubMed]

J. Y. Kim, C. Lee, K. Park, G. Lim, and C. Kim, “Fast optical-resolution photoacoustic microscopy using a 2-axis water-proofing MEMS scanner,” Sci. Rep. 5(1), 7932 (2015).
[Crossref] [PubMed]

Park, S.

S. Park, C. Lee, J. Kim, and C. Kim, “Acoustic resolution photoacoustic microscopy,” Biomed. Eng. Lett. 4(3), 213–222 (2014).
[Crossref]

Pedley, R. B.

E. Z. Zhang, J. G. Laufer, R. B. Pedley, and P. C. Beard, “In vivo high-resolution 3D photoacoustic imaging of superficial vascular anatomy,” Phys. Med. Biol. 54(4), 1035–1046 (2009).
[Crossref] [PubMed]

Pramanik, M.

M. Moothanchery and M. Pramanik, “Performance Characterization of a Switchable Acoustic Resolution and Optical Resolution Photoacoustic Microscopy System,” Sensors (Basel) 17(2), 357 (2017).
[Crossref] [PubMed]

M. Moothanchery, R. Z. Seeni, C. Xu, and M. Pramanik, “In vivo studies of transdermal nanoparticle delivery with microneedles using photoacoustic microscopy,” Biomed. Opt. Express 8(12), 5483–5492 (2017).
[Crossref] [PubMed]

M. Moothanchery, A. Sharma, and M. Pramanik, “Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for in vivo small-animal blood vasculature imaging,” J. Vis. Exp. 124, e55810 (2017).
[PubMed]

Psaltis, D.

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett. 102(21), 211106 (2013).
[Crossref]

Seeni, R. Z.

Shao, P.

P. Shao, W. Shi, P. Hajireza, and R. J. Zemp, “Integrated micro-endoscopy system for simultaneous fluorescence and optical-resolution photoacoustic imaging,” J. Biomed. Opt. 17(7), 076024 (2012).
[Crossref] [PubMed]

Sharma, A.

M. Moothanchery, A. Sharma, and M. Pramanik, “Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for in vivo small-animal blood vasculature imaging,” J. Vis. Exp. 124, e55810 (2017).
[PubMed]

Shi, W.

P. Shao, W. Shi, P. Hajireza, and R. J. Zemp, “Integrated micro-endoscopy system for simultaneous fluorescence and optical-resolution photoacoustic imaging,” J. Biomed. Opt. 17(7), 076024 (2012).
[Crossref] [PubMed]

Shung, K. K.

Simandoux, O.

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett. 102(21), 211106 (2013).
[Crossref]

Song, H. B.

S. Jeon, H. B. Song, J. Kim, B. J. Lee, R. Managuli, J. H. Kim, J. H. Kim, and C. Kim, “In Vivo Photoacoustic Imaging of Anterior Ocular Vasculature: A Random Sample Consensus Approach,” Sci. Rep. 7(1), 4318 (2017).
[Crossref] [PubMed]

Song, L.

Song, W.

Stoica, G.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, “Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging,” Nat. Biotechnol. 24(7), 848–851 (2006).
[Crossref] [PubMed]

K. Maslov, G. Stoica, and L. V. Wang, “In vivo dark-field reflection-mode photoacoustic microscopy,” Opt. Lett. 30(6), 625–627 (2005).
[Crossref] [PubMed]

Wang, L.

J. Yao, C.-H. Huang, L. Wang, J.-M. Yang, L. Gao, K. I. Maslov, J. Zou, and L. V. Wang, “Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror,” J. Biomed. Opt. 17(8), 080505 (2012).
[Crossref] [PubMed]

Wang, L. V.

L. V. Wang and J. Yao, “A practical guide to photoacoustic tomography in the life sciences,” Nat. Methods 13(8), 627–638 (2016).
[Crossref] [PubMed]

Y. Zhou, J. Yao, and L. V. Wang, “Tutorial on photoacoustic tomography,” J. Biomed. Opt. 21(6), 061007 (2016).
[Crossref] [PubMed]

J. Yao and L. V. Wang, “Photoacoustic Brain Imaging: from Microscopic to Macroscopic Scales,” Neurophotonics 1(1), 011003 (2014).
[Crossref] [PubMed]

L. V. Wang and S. Hu, “Photoacoustic Tomography: In Vivo Imaging from Organelles to Organs,” Science 335(6075), 1458–1462 (2012).
[Crossref] [PubMed]

J. Yao, C.-H. Huang, L. Wang, J.-M. Yang, L. Gao, K. I. Maslov, J. Zou, and L. V. Wang, “Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror,” J. Biomed. Opt. 17(8), 080505 (2012).
[Crossref] [PubMed]

J.-M. Yang, R. Chen, C. Favazza, J. Yao, C. Li, Z. Hu, Q. Zhou, K. K. Shung, and L. V. Wang, “A 2.5-mm diameter probe for photoacoustic and ultrasonic endoscopy,” Opt. Express 20(21), 23944–23953 (2012).
[Crossref] [PubMed]

S. Hu, K. Maslov, and L. V. Wang, “Second-generation optical-resolution photoacoustic microscopy with improved sensitivity and speed,” Opt. Lett. 36(7), 1134–1136 (2011).
[Crossref] [PubMed]

L. V. Wang, “Multiscale photoacoustic microscopy and computed tomography,” Nat. Photonics 3(9), 503–509 (2009).
[Crossref] [PubMed]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, “Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging,” Nat. Biotechnol. 24(7), 848–851 (2006).
[Crossref] [PubMed]

K. Maslov, G. Stoica, and L. V. Wang, “In vivo dark-field reflection-mode photoacoustic microscopy,” Opt. Lett. 30(6), 625–627 (2005).
[Crossref] [PubMed]

Xing, D.

Xu, C.

Yang, G.-Z.

Yang, J.-M.

J. Yao, C.-H. Huang, L. Wang, J.-M. Yang, L. Gao, K. I. Maslov, J. Zou, and L. V. Wang, “Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror,” J. Biomed. Opt. 17(8), 080505 (2012).
[Crossref] [PubMed]

J.-M. Yang, R. Chen, C. Favazza, J. Yao, C. Li, Z. Hu, Q. Zhou, K. K. Shung, and L. V. Wang, “A 2.5-mm diameter probe for photoacoustic and ultrasonic endoscopy,” Opt. Express 20(21), 23944–23953 (2012).
[Crossref] [PubMed]

Yang, S.

Yao, J.

Y. Zhou, J. Yao, and L. V. Wang, “Tutorial on photoacoustic tomography,” J. Biomed. Opt. 21(6), 061007 (2016).
[Crossref] [PubMed]

L. V. Wang and J. Yao, “A practical guide to photoacoustic tomography in the life sciences,” Nat. Methods 13(8), 627–638 (2016).
[Crossref] [PubMed]

J. Yao and L. V. Wang, “Photoacoustic Brain Imaging: from Microscopic to Macroscopic Scales,” Neurophotonics 1(1), 011003 (2014).
[Crossref] [PubMed]

J.-M. Yang, R. Chen, C. Favazza, J. Yao, C. Li, Z. Hu, Q. Zhou, K. K. Shung, and L. V. Wang, “A 2.5-mm diameter probe for photoacoustic and ultrasonic endoscopy,” Opt. Express 20(21), 23944–23953 (2012).
[Crossref] [PubMed]

J. Yao, C.-H. Huang, L. Wang, J.-M. Yang, L. Gao, K. I. Maslov, J. Zou, and L. V. Wang, “Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror,” J. Biomed. Opt. 17(8), 080505 (2012).
[Crossref] [PubMed]

Yuan, Y.

Zemp, R. J.

P. Shao, W. Shi, P. Hajireza, and R. J. Zemp, “Integrated micro-endoscopy system for simultaneous fluorescence and optical-resolution photoacoustic imaging,” J. Biomed. Opt. 17(7), 076024 (2012).
[Crossref] [PubMed]

Zhang, E. Z.

E. Z. Zhang, J. G. Laufer, R. B. Pedley, and P. C. Beard, “In vivo high-resolution 3D photoacoustic imaging of superficial vascular anatomy,” Phys. Med. Biol. 54(4), 1035–1046 (2009).
[Crossref] [PubMed]

Zhang, H. F.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, “Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging,” Nat. Biotechnol. 24(7), 848–851 (2006).
[Crossref] [PubMed]

Zhang, R.

Zheng, W.

Zhou, Q.

Zhou, Y.

Y. Zhou, J. Yao, and L. V. Wang, “Tutorial on photoacoustic tomography,” J. Biomed. Opt. 21(6), 061007 (2016).
[Crossref] [PubMed]

Zou, J.

J. Yao, C.-H. Huang, L. Wang, J.-M. Yang, L. Gao, K. I. Maslov, J. Zou, and L. V. Wang, “Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror,” J. Biomed. Opt. 17(8), 080505 (2012).
[Crossref] [PubMed]

Appl. Phys. Lett. (1)

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett. 102(21), 211106 (2013).
[Crossref]

Biomed. Eng. Lett. (1)

S. Park, C. Lee, J. Kim, and C. Kim, “Acoustic resolution photoacoustic microscopy,” Biomed. Eng. Lett. 4(3), 213–222 (2014).
[Crossref]

Biomed. Opt. Express (4)

Interface Focus (1)

P. Beard, “Biomedical photoacoustic imaging,” Interface Focus 1(4), 602–631 (2011).
[Crossref] [PubMed]

J. Biomed. Opt. (3)

Y. Zhou, J. Yao, and L. V. Wang, “Tutorial on photoacoustic tomography,” J. Biomed. Opt. 21(6), 061007 (2016).
[Crossref] [PubMed]

J. Yao, C.-H. Huang, L. Wang, J.-M. Yang, L. Gao, K. I. Maslov, J. Zou, and L. V. Wang, “Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror,” J. Biomed. Opt. 17(8), 080505 (2012).
[Crossref] [PubMed]

P. Shao, W. Shi, P. Hajireza, and R. J. Zemp, “Integrated micro-endoscopy system for simultaneous fluorescence and optical-resolution photoacoustic imaging,” J. Biomed. Opt. 17(7), 076024 (2012).
[Crossref] [PubMed]

J. Vis. Exp. (1)

M. Moothanchery, A. Sharma, and M. Pramanik, “Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for in vivo small-animal blood vasculature imaging,” J. Vis. Exp. 124, e55810 (2017).
[PubMed]

Nat. Biotechnol. (1)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, “Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging,” Nat. Biotechnol. 24(7), 848–851 (2006).
[Crossref] [PubMed]

Nat. Commun. (1)

T. Čižmár and K. Dholakia, “Exploiting multimode waveguides for pure fibre-based imaging,” Nat. Commun. 3, 1027 (2012).
[Crossref] [PubMed]

Nat. Methods (1)

L. V. Wang and J. Yao, “A practical guide to photoacoustic tomography in the life sciences,” Nat. Methods 13(8), 627–638 (2016).
[Crossref] [PubMed]

Nat. Photonics (1)

L. V. Wang, “Multiscale photoacoustic microscopy and computed tomography,” Nat. Photonics 3(9), 503–509 (2009).
[Crossref] [PubMed]

Neurophotonics (1)

J. Yao and L. V. Wang, “Photoacoustic Brain Imaging: from Microscopic to Macroscopic Scales,” Neurophotonics 1(1), 011003 (2014).
[Crossref] [PubMed]

Opt. Express (2)

Opt. Lett. (3)

Phys. Med. Biol. (1)

E. Z. Zhang, J. G. Laufer, R. B. Pedley, and P. C. Beard, “In vivo high-resolution 3D photoacoustic imaging of superficial vascular anatomy,” Phys. Med. Biol. 54(4), 1035–1046 (2009).
[Crossref] [PubMed]

Sci. Rep. (3)

S. Jeon, H. B. Song, J. Kim, B. J. Lee, R. Managuli, J. H. Kim, J. H. Kim, and C. Kim, “In Vivo Photoacoustic Imaging of Anterior Ocular Vasculature: A Random Sample Consensus Approach,” Sci. Rep. 7(1), 4318 (2017).
[Crossref] [PubMed]

J. Y. Kim, C. Lee, K. Park, G. Lim, and C. Kim, “Fast optical-resolution photoacoustic microscopy using a 2-axis water-proofing MEMS scanner,” Sci. Rep. 5(1), 7932 (2015).
[Crossref] [PubMed]

K. Park, J. Y. Kim, C. Lee, S. Jeon, G. Lim, and C. Kim, “Handheld Photoacoustic Microscopy Probe,” Sci. Rep. 7(1), 13359 (2017).
[Crossref] [PubMed]

Science (1)

L. V. Wang and S. Hu, “Photoacoustic Tomography: In Vivo Imaging from Organelles to Organs,” Science 335(6075), 1458–1462 (2012).
[Crossref] [PubMed]

Sensors (Basel) (1)

M. Moothanchery and M. Pramanik, “Performance Characterization of a Switchable Acoustic Resolution and Optical Resolution Photoacoustic Microscopy System,” Sensors (Basel) 17(2), 357 (2017).
[Crossref] [PubMed]

Other (1)

S. Turtaev, T. Leite Ivo, and T. Čižmár, “Multimode fibres for micro-endoscopy,” in Optofluidics, Microfluidics and Nanofluidics, (deGruyer, 2015), p. 31.

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Figures (5)

Fig. 1
Fig. 1 (a) Schematic of the OR-PAM imaging system. L1- Bi-Concave lens, L2- Plano convex lens, NDF- Neutral density filter, FC - Fiber coupler, UST - Ultrasound transducer, MMF - Multimode fiber, DAQ - Data acquisition card, L3 & L4 - Achromatic lens, CL- Correction lens, AL - Acoustic lens.
Fig. 2
Fig. 2 Lateral resolution test of the OR-PAM system: (a) OR-PAM image of an Air force resolution test target, (b) OR-PAM image of the region of interest (white dotted area in (a)).
Fig. 3
Fig. 3 Spatial resolution and imaging depth test of the OR-PAM system: Measurement of the line spread function (LSF) using the edge of a USAF resolution target. Black (■) dots: photoacoustic signal; red line: edge spread function (ESF); blue line: line spread function (LSF): Inlet shows MAP image of a resolution target (b) photoacoustic axial line of a 6 μm carbon fiber, Inlet shows the B-scan axial image of the carbon fiber (c) Single B-scan PA image of a black needle inserted in a chicken tissue.
Fig. 4
Fig. 4 In vivo photoacoustic image of mouse ear: (a) OR-PAM image, using a 10 µm MM fiber at 10 kHz LRR (b) OR-PAM image, using a 25µm MM fiber at 30 kHz LRR.
Fig. 5
Fig. 5 In vivo photoacoustic image of mouse ear using: (a) SM fiber, (b) 10 μm MM fiber (c) 25 μm MM fiber.

Tables (1)

Tables Icon

Table 1 Comparison between single mode and multimode fibers

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