Abstract

Cell-based assay based on time-stretch imaging is recognized to be well-suited for high-throughput phenotypic screening. However, this ultrafast imaging technique has primarily been limited to suspension-cell assay, leaving a wide range of solid-substrate assay formats uncharted. Moreover, time-stretch imaging is generally restricted to intrinsic biophysical phenotyping, but lacks the biomolecular signatures of the cells. To address these challenges, we develop a spinning time-stretch imaging assay platform based on the functionalized digital versatile disc (DVD). We demonstrate that adherent cell culture and biochemically-specific cell-capture can now be assayed with time-stretch microscopy, thanks to the high-speed DVD spinning motion that naturally enables on-the-fly cellular imaging at an ultrafast line-scan rate of >10MHz. As scanning the whole DVD at such a high speed enables ultra-large field-of-view imaging, it could be favorable for scaling both the assay throughput and content as demanded in many applications, e.g. drug discovery, and rare cancer cell screening.

© 2017 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
High-throughput time-stretch imaging flow cytometry for multi-class classification of phytoplankton

Queenie T. K. Lai, Kelvin C. M. Lee, Anson H. L. Tang, Kenneth K. Y. Wong, Hayden K. H. So, and Kevin K. Tsia
Opt. Express 24(25) 28170-28184 (2016)

High-throughput label-free image cytometry and image-based classification of live Euglena gracilis

Cheng Lei, Takuro Ito, Masashi Ugawa, Taisuke Nozawa, Osamu Iwata, Masanori Maki, Genki Okada, Hirofumi Kobayashi, Xinlei Sun, Pimsiri Tiamsak, Norimichi Tsumura, Kengo Suzuki, Dino Di Carlo, Yasuyuki Ozeki, and Keisuke Goda
Biomed. Opt. Express 7(7) 2703-2708 (2016)

SPIM-fluid: open source light-sheet based platform for high-throughput imaging

Emilio J. Gualda, Hugo Pereira, Tiago Vale, Marta Falcão Estrada, Catarina Brito, and Nuno Moreno
Biomed. Opt. Express 6(11) 4447-4456 (2015)

References

  • View by:
  • |
  • |
  • |

  1. M. Boutros, F. Heigwer, and C. Laufer, “Microscopy-Based High-Content Screening,” Cell 163(6), 1314–1325 (2015).
    [Crossref] [PubMed]
  2. I. A. V. Natasha S. Barteneva, Imaging Flow Cytometry: Methods and Protocols, 1st ed., Methods in Molecular Biology (Humana Press, 2016).
  3. C. Alix-Panabières and K. Pantel, “Challenges in circulating tumour cell research,” Nat. Rev. Cancer 14(9), 623–631 (2014).
    [Crossref] [PubMed]
  4. Y. Feng, T. J. Mitchison, A. Bender, D. W. Young, and J. A. Tallarico, “Multi-parameter phenotypic profiling: using cellular effects to characterize small-molecule compounds,” Nat. Rev. Drug Discov. 8(7), 567–578 (2009).
    [Crossref] [PubMed]
  5. P. Lang, K. Yeow, A. Nichols, and A. Scheer, “Cellular imaging in drug discovery,” Nat. Rev. Drug Discov. 5(4), 343–356 (2006).
    [Crossref] [PubMed]
  6. K. Goda and B. Jalali, “Dispersive Fourier transformation for fast continuous single-shot measurements,” Nat. Photonics 7(2), 102 (2013).
    [Crossref]
  7. A. K. Lau, H. C. Shum, K. K. Wong, and K. K. Tsia, “Optofluidic time-stretch imaging - an emerging tool for high-throughput imaging flow cytometry,” Lab Chip 16(10), 1743–1756 (2016).
    [Crossref] [PubMed]
  8. K. Goda, K. K. Tsia, and B. Jalali, “Serial time-encoded amplified imaging for real-time observation of fast dynamic phenomena,” Nature 458(7242), 1145–1149 (2009).
    [Crossref] [PubMed]
  9. C. Lei, B. Guo, Z. Cheng, and K. Goda, “Optical time-stretch imaging: Principles and applications,” Appl. Phys. Rev. 3(1), 011102 (2016).
    [Crossref]
  10. K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
    [Crossref] [PubMed]
  11. C. L. Chen, A. Mahjoubfar, L. C. Tai, I. K. Blaby, A. Huang, K. R. Niazi, and B. Jalali, “Deep Learning in Label-free Cell Classification,” Sci. Rep. 6, 21471 (2016).
    [Crossref] [PubMed]
  12. A. K. Lau, T. T. Wong, K. K. Ho, M. T. Tang, A. C. Chan, X. Wei, E. Y. Lam, H. C. Shum, K. K. Wong, and K. K. Tsia, “Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm,” J. Biomed. Opt. 19(7), 076001 (2014).
    [Crossref] [PubMed]
  13. R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. S. Lee, J. M. Park, J. Kim, H. Kim, M. Madou, and Y. K. Cho, “Centrifugal microfluidics for biomedical applications,” Lab Chip 10(14), 1758–1773 (2010).
    [Crossref] [PubMed]
  14. O. Strohmeier, M. Keller, F. Schwemmer, S. Zehnle, D. Mark, F. von Stetten, R. Zengerle, and N. Paust, “Centrifugal microfluidic platforms: advanced unit operations and applications,” Chem. Soc. Rev. 44(17), 6187–6229 (2015).
    [Crossref] [PubMed]
  15. L. Wang and P. C. Li, “Microfluidic DNA microarray analysis: a review,” Anal. Chim. Acta 687(1), 12–27 (2011).
    [Crossref] [PubMed]
  16. J. Park, V. Sunkara, T. H. Kim, H. Hwang, and Y. K. Cho, “Lab-on-a-disc for fully integrated multiplex immunoassays,” Anal. Chem. 84(5), 2133–2140 (2012).
    [Crossref] [PubMed]
  17. H. Ramachandraiah, M. Amasia, J. Cole, P. Sheard, S. Pickhaver, C. Walker, V. Wirta, P. Lexow, R. Lione, and A. Russom, “Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics,” Lab Chip 13(8), 1578–1585 (2013).
    [Crossref] [PubMed]
  18. A. K. S. Lau, A. H. L. Tang, X. W. J. Xu, K. K. Y. Wong, and K. K. Tsia, “Optical Time Stretch for High-Speed and High-Throughput Imaging — From Single-Cell to Tissue-Wide Scales,” IEEE J. Sel. Top. Quantum Electron. 22, 6803115 (2016).
  19. K. K. Tsia, K. Goda, D. Capewell, and B. Jalali, “Performance of serial time-encoded amplified microscope,” Opt. Express 18(10), 10016–10028 (2010).
    [Crossref] [PubMed]
  20. T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
    [Crossref] [PubMed]
  21. E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
    [Crossref] [PubMed]
  22. S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
    [Crossref] [PubMed]
  23. O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
    [Crossref] [PubMed]
  24. T. A. Zangle and M. A. Teitell, “Live-cell mass profiling: an emerging approach in quantitative biophysics,” Nat. Methods 11(12), 1221–1228 (2014).
    [Crossref] [PubMed]
  25. H. T. Tse, D. R. Gossett, Y. S. Moon, M. Masaeli, M. Sohsman, Y. Ying, K. Mislick, R. P. Adams, J. Rao, and D. Di Carlo, “Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping,” Sci. Transl. Med. 5(212), 212ra163 (2013).
    [Crossref] [PubMed]
  26. A. W. Lohmann, R. G. Dorsch, D. Mendlovic, C. Ferreira, and Z. Zalevsky, “Space–bandwidth product of optical signals and systems,” J. Opt. Soc. Am. A 13(3), 470–473 (1996).
    [Crossref]
  27. J. Wu, Y. Xu, J. Xu, X. Wei, A. C. Chan, A. H. Tang, A. K. Lau, B. M. Chung, H. C. Shum, E. Y. Lam, K. K. Wong and K. K. Tsia, Ultrafast Laser-Scanning Time-Stretch Imaging at Visible Wavelengths. Light: Science & Applications accepted article preview 12 August 2016.
  28. D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
    [Crossref] [PubMed]

2016 (4)

A. K. Lau, H. C. Shum, K. K. Wong, and K. K. Tsia, “Optofluidic time-stretch imaging - an emerging tool for high-throughput imaging flow cytometry,” Lab Chip 16(10), 1743–1756 (2016).
[Crossref] [PubMed]

C. Lei, B. Guo, Z. Cheng, and K. Goda, “Optical time-stretch imaging: Principles and applications,” Appl. Phys. Rev. 3(1), 011102 (2016).
[Crossref]

C. L. Chen, A. Mahjoubfar, L. C. Tai, I. K. Blaby, A. Huang, K. R. Niazi, and B. Jalali, “Deep Learning in Label-free Cell Classification,” Sci. Rep. 6, 21471 (2016).
[Crossref] [PubMed]

A. K. S. Lau, A. H. L. Tang, X. W. J. Xu, K. K. Y. Wong, and K. K. Tsia, “Optical Time Stretch for High-Speed and High-Throughput Imaging — From Single-Cell to Tissue-Wide Scales,” IEEE J. Sel. Top. Quantum Electron. 22, 6803115 (2016).

2015 (4)

O. Strohmeier, M. Keller, F. Schwemmer, S. Zehnle, D. Mark, F. von Stetten, R. Zengerle, and N. Paust, “Centrifugal microfluidic platforms: advanced unit operations and applications,” Chem. Soc. Rev. 44(17), 6187–6229 (2015).
[Crossref] [PubMed]

M. Boutros, F. Heigwer, and C. Laufer, “Microscopy-Based High-Content Screening,” Cell 163(6), 1314–1325 (2015).
[Crossref] [PubMed]

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

2014 (4)

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

T. A. Zangle and M. A. Teitell, “Live-cell mass profiling: an emerging approach in quantitative biophysics,” Nat. Methods 11(12), 1221–1228 (2014).
[Crossref] [PubMed]

C. Alix-Panabières and K. Pantel, “Challenges in circulating tumour cell research,” Nat. Rev. Cancer 14(9), 623–631 (2014).
[Crossref] [PubMed]

A. K. Lau, T. T. Wong, K. K. Ho, M. T. Tang, A. C. Chan, X. Wei, E. Y. Lam, H. C. Shum, K. K. Wong, and K. K. Tsia, “Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm,” J. Biomed. Opt. 19(7), 076001 (2014).
[Crossref] [PubMed]

2013 (4)

K. Goda and B. Jalali, “Dispersive Fourier transformation for fast continuous single-shot measurements,” Nat. Photonics 7(2), 102 (2013).
[Crossref]

H. T. Tse, D. R. Gossett, Y. S. Moon, M. Masaeli, M. Sohsman, Y. Ying, K. Mislick, R. P. Adams, J. Rao, and D. Di Carlo, “Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping,” Sci. Transl. Med. 5(212), 212ra163 (2013).
[Crossref] [PubMed]

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

H. Ramachandraiah, M. Amasia, J. Cole, P. Sheard, S. Pickhaver, C. Walker, V. Wirta, P. Lexow, R. Lione, and A. Russom, “Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics,” Lab Chip 13(8), 1578–1585 (2013).
[Crossref] [PubMed]

2012 (2)

J. Park, V. Sunkara, T. H. Kim, H. Hwang, and Y. K. Cho, “Lab-on-a-disc for fully integrated multiplex immunoassays,” Anal. Chem. 84(5), 2133–2140 (2012).
[Crossref] [PubMed]

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

2011 (1)

L. Wang and P. C. Li, “Microfluidic DNA microarray analysis: a review,” Anal. Chim. Acta 687(1), 12–27 (2011).
[Crossref] [PubMed]

2010 (2)

R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. S. Lee, J. M. Park, J. Kim, H. Kim, M. Madou, and Y. K. Cho, “Centrifugal microfluidics for biomedical applications,” Lab Chip 10(14), 1758–1773 (2010).
[Crossref] [PubMed]

K. K. Tsia, K. Goda, D. Capewell, and B. Jalali, “Performance of serial time-encoded amplified microscope,” Opt. Express 18(10), 10016–10028 (2010).
[Crossref] [PubMed]

2009 (2)

K. Goda, K. K. Tsia, and B. Jalali, “Serial time-encoded amplified imaging for real-time observation of fast dynamic phenomena,” Nature 458(7242), 1145–1149 (2009).
[Crossref] [PubMed]

Y. Feng, T. J. Mitchison, A. Bender, D. W. Young, and J. A. Tallarico, “Multi-parameter phenotypic profiling: using cellular effects to characterize small-molecule compounds,” Nat. Rev. Drug Discov. 8(7), 567–578 (2009).
[Crossref] [PubMed]

2007 (1)

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

2006 (1)

P. Lang, K. Yeow, A. Nichols, and A. Scheer, “Cellular imaging in drug discovery,” Nat. Rev. Drug Discov. 5(4), 343–356 (2006).
[Crossref] [PubMed]

1996 (1)

Adam, J.

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

Adams, R. P.

H. T. Tse, D. R. Gossett, Y. S. Moon, M. Masaeli, M. Sohsman, Y. Ying, K. Mislick, R. P. Adams, J. Rao, and D. Di Carlo, “Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping,” Sci. Transl. Med. 5(212), 212ra163 (2013).
[Crossref] [PubMed]

Alix-Panabières, C.

C. Alix-Panabières and K. Pantel, “Challenges in circulating tumour cell research,” Nat. Rev. Cancer 14(9), 623–631 (2014).
[Crossref] [PubMed]

Amasia, M.

H. Ramachandraiah, M. Amasia, J. Cole, P. Sheard, S. Pickhaver, C. Walker, V. Wirta, P. Lexow, R. Lione, and A. Russom, “Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics,” Lab Chip 13(8), 1578–1585 (2013).
[Crossref] [PubMed]

R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. S. Lee, J. M. Park, J. Kim, H. Kim, M. Madou, and Y. K. Cho, “Centrifugal microfluidics for biomedical applications,” Lab Chip 10(14), 1758–1773 (2010).
[Crossref] [PubMed]

Ayazi, A.

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

Balakrishnan, S.

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

Barber, T. A.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Bardia, A.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Beck, K.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Bender, A.

Y. Feng, T. J. Mitchison, A. Bender, D. W. Young, and J. A. Tallarico, “Multi-parameter phenotypic profiling: using cellular effects to characterize small-molecule compounds,” Nat. Rev. Drug Discov. 8(7), 567–578 (2009).
[Crossref] [PubMed]

Bhakta, N. R.

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

Blaby, I. K.

C. L. Chen, A. Mahjoubfar, L. C. Tai, I. K. Blaby, A. Huang, K. R. Niazi, and B. Jalali, “Deep Learning in Label-free Cell Classification,” Sci. Rep. 6, 21471 (2016).
[Crossref] [PubMed]

Boutros, M.

M. Boutros, F. Heigwer, and C. Laufer, “Microscopy-Based High-Content Screening,” Cell 163(6), 1314–1325 (2015).
[Crossref] [PubMed]

Brachtel, E.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Brackbill, N.

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

Capewell, D.

Chan, A. C.

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

A. K. Lau, T. T. Wong, K. K. Ho, M. T. Tang, A. C. Chan, X. Wei, E. Y. Lam, H. C. Shum, K. K. Wong, and K. K. Tsia, “Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm,” J. Biomed. Opt. 19(7), 076001 (2014).
[Crossref] [PubMed]

Chan, G. C.

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

Chen, C. L.

C. L. Chen, A. Mahjoubfar, L. C. Tai, I. K. Blaby, A. Huang, K. R. Niazi, and B. Jalali, “Deep Learning in Label-free Cell Classification,” Sci. Rep. 6, 21471 (2016).
[Crossref] [PubMed]

Chen, P. I.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Cheng, Z.

C. Lei, B. Guo, Z. Cheng, and K. Goda, “Optical time-stretch imaging: Principles and applications,” Appl. Phys. Rev. 3(1), 011102 (2016).
[Crossref]

Cho, Y. K.

J. Park, V. Sunkara, T. H. Kim, H. Hwang, and Y. K. Cho, “Lab-on-a-disc for fully integrated multiplex immunoassays,” Anal. Chem. 84(5), 2133–2140 (2012).
[Crossref] [PubMed]

R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. S. Lee, J. M. Park, J. Kim, H. Kim, M. Madou, and Y. K. Cho, “Centrifugal microfluidics for biomedical applications,” Lab Chip 10(14), 1758–1773 (2010).
[Crossref] [PubMed]

Ciciliano, J. C.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Coith, C.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Cole, J.

H. Ramachandraiah, M. Amasia, J. Cole, P. Sheard, S. Pickhaver, C. Walker, V. Wirta, P. Lexow, R. Lione, and A. Russom, “Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics,” Lab Chip 13(8), 1578–1585 (2013).
[Crossref] [PubMed]

Cristofanilli, M.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Di Carlo, D.

H. T. Tse, D. R. Gossett, Y. S. Moon, M. Masaeli, M. Sohsman, Y. Ying, K. Mislick, R. P. Adams, J. Rao, and D. Di Carlo, “Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping,” Sci. Transl. Med. 5(212), 212ra163 (2013).
[Crossref] [PubMed]

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

Dorsch, R. G.

Ekpenyong, A.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Emmink, B. L.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Eyob, H.

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

Fard, A. M.

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

Feng, Y.

Y. Feng, T. J. Mitchison, A. Bender, D. W. Young, and J. A. Tallarico, “Multi-parameter phenotypic profiling: using cellular effects to characterize small-molecule compounds,” Nat. Rev. Drug Discov. 8(7), 567–578 (2009).
[Crossref] [PubMed]

Ferreira, C.

Fischer-Friedrich, E.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Fritsche, H.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Girardo, S.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Goda, K.

C. Lei, B. Guo, Z. Cheng, and K. Goda, “Optical time-stretch imaging: Principles and applications,” Appl. Phys. Rev. 3(1), 011102 (2016).
[Crossref]

K. Goda and B. Jalali, “Dispersive Fourier transformation for fast continuous single-shot measurements,” Nat. Photonics 7(2), 102 (2013).
[Crossref]

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

K. K. Tsia, K. Goda, D. Capewell, and B. Jalali, “Performance of serial time-encoded amplified microscope,” Opt. Express 18(10), 10016–10028 (2010).
[Crossref] [PubMed]

K. Goda, K. K. Tsia, and B. Jalali, “Serial time-encoded amplified imaging for real-time observation of fast dynamic phenomena,” Nature 458(7242), 1145–1149 (2009).
[Crossref] [PubMed]

Goga, A.

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

Golfier, S.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Gorkin, R.

R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. S. Lee, J. M. Park, J. Kim, H. Kim, M. Madou, and Y. K. Cho, “Centrifugal microfluidics for biomedical applications,” Lab Chip 10(14), 1758–1773 (2010).
[Crossref] [PubMed]

Gornet, T.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Gossett, D. R.

H. T. Tse, D. R. Gossett, Y. S. Moon, M. Masaeli, M. Sohsman, Y. Ying, K. Mislick, R. P. Adams, J. Rao, and D. Di Carlo, “Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping,” Sci. Transl. Med. 5(212), 212ra163 (2013).
[Crossref] [PubMed]

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

Guck, J.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Guo, B.

C. Lei, B. Guo, Z. Cheng, and K. Goda, “Optical time-stretch imaging: Principles and applications,” Appl. Phys. Rev. 3(1), 011102 (2016).
[Crossref]

Haber, D. A.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Heigwer, F.

M. Boutros, F. Heigwer, and C. Laufer, “Microscopy-Based High-Content Screening,” Cell 163(6), 1314–1325 (2015).
[Crossref] [PubMed]

Herold, C.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Ho, K. K.

A. K. Lau, T. T. Wong, K. K. Ho, M. T. Tang, A. C. Chan, X. Wei, E. Y. Lam, H. C. Shum, K. K. Wong, and K. K. Tsia, “Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm,” J. Biomed. Opt. 19(7), 076001 (2014).
[Crossref] [PubMed]

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

Huang, A.

C. L. Chen, A. Mahjoubfar, L. C. Tai, I. K. Blaby, A. Huang, K. R. Niazi, and B. Jalali, “Deep Learning in Label-free Cell Classification,” Sci. Rep. 6, 21471 (2016).
[Crossref] [PubMed]

Hur, S. C.

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

Hwang, H.

J. Park, V. Sunkara, T. H. Kim, H. Hwang, and Y. K. Cho, “Lab-on-a-disc for fully integrated multiplex immunoassays,” Anal. Chem. 84(5), 2133–2140 (2012).
[Crossref] [PubMed]

Jackson, S.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Jacobi, A.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Jalali, B.

C. L. Chen, A. Mahjoubfar, L. C. Tai, I. K. Blaby, A. Huang, K. R. Niazi, and B. Jalali, “Deep Learning in Label-free Cell Classification,” Sci. Rep. 6, 21471 (2016).
[Crossref] [PubMed]

K. Goda and B. Jalali, “Dispersive Fourier transformation for fast continuous single-shot measurements,” Nat. Photonics 7(2), 102 (2013).
[Crossref]

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

K. K. Tsia, K. Goda, D. Capewell, and B. Jalali, “Performance of serial time-encoded amplified microscope,” Opt. Express 18(10), 10016–10028 (2010).
[Crossref] [PubMed]

K. Goda, K. K. Tsia, and B. Jalali, “Serial time-encoded amplified imaging for real-time observation of fast dynamic phenomena,” Nature 458(7242), 1145–1149 (2009).
[Crossref] [PubMed]

Jänicke, F.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Janni, W.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Kapur, R.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Karabacak, N. M.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Keller, M.

O. Strohmeier, M. Keller, F. Schwemmer, S. Zehnle, D. Mark, F. von Stetten, R. Zengerle, and N. Paust, “Centrifugal microfluidic platforms: advanced unit operations and applications,” Chem. Soc. Rev. 44(17), 6187–6229 (2015).
[Crossref] [PubMed]

Kessenbrock, K.

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

Keyser, U. F.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Kim, H.

R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. S. Lee, J. M. Park, J. Kim, H. Kim, M. Madou, and Y. K. Cho, “Centrifugal microfluidics for biomedical applications,” Lab Chip 10(14), 1758–1773 (2010).
[Crossref] [PubMed]

Kim, J.

R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. S. Lee, J. M. Park, J. Kim, H. Kim, M. Madou, and Y. K. Cho, “Centrifugal microfluidics for biomedical applications,” Lab Chip 10(14), 1758–1773 (2010).
[Crossref] [PubMed]

Kim, T. H.

J. Park, V. Sunkara, T. H. Kim, H. Hwang, and Y. K. Cho, “Lab-on-a-disc for fully integrated multiplex immunoassays,” Anal. Chem. 84(5), 2133–2140 (2012).
[Crossref] [PubMed]

Kimura, A.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Klaue, D.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Lam, E. Y.

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

A. K. Lau, T. T. Wong, K. K. Ho, M. T. Tang, A. C. Chan, X. Wei, E. Y. Lam, H. C. Shum, K. K. Wong, and K. K. Tsia, “Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm,” J. Biomed. Opt. 19(7), 076001 (2014).
[Crossref] [PubMed]

Lang, P.

P. Lang, K. Yeow, A. Nichols, and A. Scheer, “Cellular imaging in drug discovery,” Nat. Rev. Drug Discov. 5(4), 343–356 (2006).
[Crossref] [PubMed]

Lau, A. K.

A. K. Lau, H. C. Shum, K. K. Wong, and K. K. Tsia, “Optofluidic time-stretch imaging - an emerging tool for high-throughput imaging flow cytometry,” Lab Chip 16(10), 1743–1756 (2016).
[Crossref] [PubMed]

A. K. Lau, T. T. Wong, K. K. Ho, M. T. Tang, A. C. Chan, X. Wei, E. Y. Lam, H. C. Shum, K. K. Wong, and K. K. Tsia, “Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm,” J. Biomed. Opt. 19(7), 076001 (2014).
[Crossref] [PubMed]

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

Lau, A. K. S.

A. K. S. Lau, A. H. L. Tang, X. W. J. Xu, K. K. Y. Wong, and K. K. Tsia, “Optical Time Stretch for High-Speed and High-Throughput Imaging — From Single-Cell to Tissue-Wide Scales,” IEEE J. Sel. Top. Quantum Electron. 22, 6803115 (2016).

Laufer, C.

M. Boutros, F. Heigwer, and C. Laufer, “Microscopy-Based High-Content Screening,” Cell 163(6), 1314–1325 (2015).
[Crossref] [PubMed]

Lawson, D. A.

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

Lee, B. S.

R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. S. Lee, J. M. Park, J. Kim, H. Kim, M. Madou, and Y. K. Cho, “Centrifugal microfluidics for biomedical applications,” Lab Chip 10(14), 1758–1773 (2010).
[Crossref] [PubMed]

Lee, R. J.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Lei, C.

C. Lei, B. Guo, Z. Cheng, and K. Goda, “Optical time-stretch imaging: Principles and applications,” Appl. Phys. Rev. 3(1), 011102 (2016).
[Crossref]

Lexow, P.

H. Ramachandraiah, M. Amasia, J. Cole, P. Sheard, S. Pickhaver, C. Walker, V. Wirta, P. Lexow, R. Lione, and A. Russom, “Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics,” Lab Chip 13(8), 1578–1585 (2013).
[Crossref] [PubMed]

Li, P. C.

L. Wang and P. C. Li, “Microfluidic DNA microarray analysis: a review,” Anal. Chim. Acta 687(1), 12–27 (2011).
[Crossref] [PubMed]

Lione, R.

H. Ramachandraiah, M. Amasia, J. Cole, P. Sheard, S. Pickhaver, C. Walker, V. Wirta, P. Lexow, R. Lione, and A. Russom, “Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics,” Lab Chip 13(8), 1578–1585 (2013).
[Crossref] [PubMed]

Lohmann, A. W.

Lonappan, C. K.

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

Louis, D. N.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Luo, X.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Madou, M.

R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. S. Lee, J. M. Park, J. Kim, H. Kim, M. Madou, and Y. K. Cho, “Centrifugal microfluidics for biomedical applications,” Lab Chip 10(14), 1758–1773 (2010).
[Crossref] [PubMed]

Maheswaran, S.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Mahjoubfar, A.

C. L. Chen, A. Mahjoubfar, L. C. Tai, I. K. Blaby, A. Huang, K. R. Niazi, and B. Jalali, “Deep Learning in Label-free Cell Classification,” Sci. Rep. 6, 21471 (2016).
[Crossref] [PubMed]

Mansfeld, J.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Mark, D.

O. Strohmeier, M. Keller, F. Schwemmer, S. Zehnle, D. Mark, F. von Stetten, R. Zengerle, and N. Paust, “Centrifugal microfluidic platforms: advanced unit operations and applications,” Chem. Soc. Rev. 44(17), 6187–6229 (2015).
[Crossref] [PubMed]

Masaeli, M.

H. T. Tse, D. R. Gossett, Y. S. Moon, M. Masaeli, M. Sohsman, Y. Ying, K. Mislick, R. P. Adams, J. Rao, and D. Di Carlo, “Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping,” Sci. Transl. Med. 5(212), 212ra163 (2013).
[Crossref] [PubMed]

Mendlovic, D.

Mietke, A.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Mislick, K.

H. T. Tse, D. R. Gossett, Y. S. Moon, M. Masaeli, M. Sohsman, Y. Ying, K. Mislick, R. P. Adams, J. Rao, and D. Di Carlo, “Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping,” Sci. Transl. Med. 5(212), 212ra163 (2013).
[Crossref] [PubMed]

Mitchison, T. J.

Y. Feng, T. J. Mitchison, A. Bender, D. W. Young, and J. A. Tallarico, “Multi-parameter phenotypic profiling: using cellular effects to characterize small-molecule compounds,” Nat. Rev. Drug Discov. 8(7), 567–578 (2009).
[Crossref] [PubMed]

Miyamoto, D. T.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Moon, Y. S.

H. T. Tse, D. R. Gossett, Y. S. Moon, M. Masaeli, M. Sohsman, Y. Ying, K. Mislick, R. P. Adams, J. Rao, and D. Di Carlo, “Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping,” Sci. Transl. Med. 5(212), 212ra163 (2013).
[Crossref] [PubMed]

Morgan, B.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Müller, V.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Murray, C.

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

Niazi, K. R.

C. L. Chen, A. Mahjoubfar, L. C. Tai, I. K. Blaby, A. Huang, K. R. Niazi, and B. Jalali, “Deep Learning in Label-free Cell Classification,” Sci. Rep. 6, 21471 (2016).
[Crossref] [PubMed]

Nichols, A.

P. Lang, K. Yeow, A. Nichols, and A. Scheer, “Cellular imaging in drug discovery,” Nat. Rev. Drug Discov. 5(4), 343–356 (2006).
[Crossref] [PubMed]

Otto, O.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Ozkumur, E.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Pagliara, S.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Pantel, K.

C. Alix-Panabières and K. Pantel, “Challenges in circulating tumour cell research,” Nat. Rev. Cancer 14(9), 623–631 (2014).
[Crossref] [PubMed]

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Park, J.

J. Park, V. Sunkara, T. H. Kim, H. Hwang, and Y. K. Cho, “Lab-on-a-disc for fully integrated multiplex immunoassays,” Anal. Chem. 84(5), 2133–2140 (2012).
[Crossref] [PubMed]

R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. S. Lee, J. M. Park, J. Kim, H. Kim, M. Madou, and Y. K. Cho, “Centrifugal microfluidics for biomedical applications,” Lab Chip 10(14), 1758–1773 (2010).
[Crossref] [PubMed]

Park, J. M.

R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. S. Lee, J. M. Park, J. Kim, H. Kim, M. Madou, and Y. K. Cho, “Centrifugal microfluidics for biomedical applications,” Lab Chip 10(14), 1758–1773 (2010).
[Crossref] [PubMed]

Paust, N.

O. Strohmeier, M. Keller, F. Schwemmer, S. Zehnle, D. Mark, F. von Stetten, R. Zengerle, and N. Paust, “Centrifugal microfluidic platforms: advanced unit operations and applications,” Chem. Soc. Rev. 44(17), 6187–6229 (2015).
[Crossref] [PubMed]

Pickhaver, S.

H. Ramachandraiah, M. Amasia, J. Cole, P. Sheard, S. Pickhaver, C. Walker, V. Wirta, P. Lexow, R. Lione, and A. Russom, “Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics,” Lab Chip 13(8), 1578–1585 (2013).
[Crossref] [PubMed]

Prummel, K. D.

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

Rack, B.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Ramachandraiah, H.

H. Ramachandraiah, M. Amasia, J. Cole, P. Sheard, S. Pickhaver, C. Walker, V. Wirta, P. Lexow, R. Lione, and A. Russom, “Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics,” Lab Chip 13(8), 1578–1585 (2013).
[Crossref] [PubMed]

Rao, J.

H. T. Tse, D. R. Gossett, Y. S. Moon, M. Masaeli, M. Sohsman, Y. Ying, K. Mislick, R. P. Adams, J. Rao, and D. Di Carlo, “Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping,” Sci. Transl. Med. 5(212), 212ra163 (2013).
[Crossref] [PubMed]

Rau, T.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Riethdorf, S.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Robles, J. D.

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

Rosendahl, P.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Russom, A.

H. Ramachandraiah, M. Amasia, J. Cole, P. Sheard, S. Pickhaver, C. Walker, V. Wirta, P. Lexow, R. Lione, and A. Russom, “Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics,” Lab Chip 13(8), 1578–1585 (2013).
[Crossref] [PubMed]

Sadasivam, J.

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

Scheer, A.

P. Lang, K. Yeow, A. Nichols, and A. Scheer, “Cellular imaging in drug discovery,” Nat. Rev. Drug Discov. 5(4), 343–356 (2006).
[Crossref] [PubMed]

Schindlbeck, C.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Schwemmer, F.

O. Strohmeier, M. Keller, F. Schwemmer, S. Zehnle, D. Mark, F. von Stetten, R. Zengerle, and N. Paust, “Centrifugal microfluidic platforms: advanced unit operations and applications,” Chem. Soc. Rev. 44(17), 6187–6229 (2015).
[Crossref] [PubMed]

Sengupta, S.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Sequist, L. V.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Shah, A. M.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Shaw, A. T.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Sheard, P.

H. Ramachandraiah, M. Amasia, J. Cole, P. Sheard, S. Pickhaver, C. Walker, V. Wirta, P. Lexow, R. Lione, and A. Russom, “Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics,” Lab Chip 13(8), 1578–1585 (2013).
[Crossref] [PubMed]

Shum, H. C.

A. K. Lau, H. C. Shum, K. K. Wong, and K. K. Tsia, “Optofluidic time-stretch imaging - an emerging tool for high-throughput imaging flow cytometry,” Lab Chip 16(10), 1743–1756 (2016).
[Crossref] [PubMed]

A. K. Lau, T. T. Wong, K. K. Ho, M. T. Tang, A. C. Chan, X. Wei, E. Y. Lam, H. C. Shum, K. K. Wong, and K. K. Tsia, “Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm,” J. Biomed. Opt. 19(7), 076001 (2014).
[Crossref] [PubMed]

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

Siegrist, J.

R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. S. Lee, J. M. Park, J. Kim, H. Kim, M. Madou, and Y. K. Cho, “Centrifugal microfluidics for biomedical applications,” Lab Chip 10(14), 1758–1773 (2010).
[Crossref] [PubMed]

Smith, K.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Sohsman, M.

H. T. Tse, D. R. Gossett, Y. S. Moon, M. Masaeli, M. Sohsman, Y. Ying, K. Mislick, R. P. Adams, J. Rao, and D. Di Carlo, “Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping,” Sci. Transl. Med. 5(212), 212ra163 (2013).
[Crossref] [PubMed]

Sollier, E.

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

Spuhler, P. S.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Stott, S. L.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Strohmeier, O.

O. Strohmeier, M. Keller, F. Schwemmer, S. Zehnle, D. Mark, F. von Stetten, R. Zengerle, and N. Paust, “Centrifugal microfluidic platforms: advanced unit operations and applications,” Chem. Soc. Rev. 44(17), 6187–6229 (2015).
[Crossref] [PubMed]

Sullivan, J. P.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Sunkara, V.

J. Park, V. Sunkara, T. H. Kim, H. Hwang, and Y. K. Cho, “Lab-on-a-disc for fully integrated multiplex immunoassays,” Anal. Chem. 84(5), 2133–2140 (2012).
[Crossref] [PubMed]

Tai, L. C.

C. L. Chen, A. Mahjoubfar, L. C. Tai, I. K. Blaby, A. Huang, K. R. Niazi, and B. Jalali, “Deep Learning in Label-free Cell Classification,” Sci. Rep. 6, 21471 (2016).
[Crossref] [PubMed]

Takai, K.

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

Tallarico, J. A.

Y. Feng, T. J. Mitchison, A. Bender, D. W. Young, and J. A. Tallarico, “Multi-parameter phenotypic profiling: using cellular effects to characterize small-molecule compounds,” Nat. Rev. Drug Discov. 8(7), 567–578 (2009).
[Crossref] [PubMed]

Tang, A. H.

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

Tang, A. H. L.

A. K. S. Lau, A. H. L. Tang, X. W. J. Xu, K. K. Y. Wong, and K. K. Tsia, “Optical Time Stretch for High-Speed and High-Throughput Imaging — From Single-Cell to Tissue-Wide Scales,” IEEE J. Sel. Top. Quantum Electron. 22, 6803115 (2016).

Tang, M. T.

A. K. Lau, T. T. Wong, K. K. Ho, M. T. Tang, A. C. Chan, X. Wei, E. Y. Lam, H. C. Shum, K. K. Wong, and K. K. Tsia, “Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm,” J. Biomed. Opt. 19(7), 076001 (2014).
[Crossref] [PubMed]

Tang, M. Y.

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

Teitell, M. A.

T. A. Zangle and M. A. Teitell, “Live-cell mass profiling: an emerging approach in quantitative biophysics,” Nat. Methods 11(12), 1221–1228 (2014).
[Crossref] [PubMed]

Ting, D. T.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Toner, M.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Töpfner, N.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Trautwein, J.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Tse, H. T.

H. T. Tse, D. R. Gossett, Y. S. Moon, M. Masaeli, M. Sohsman, Y. Ying, K. Mislick, R. P. Adams, J. Rao, and D. Di Carlo, “Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping,” Sci. Transl. Med. 5(212), 212ra163 (2013).
[Crossref] [PubMed]

Tsia, K. K.

A. K. S. Lau, A. H. L. Tang, X. W. J. Xu, K. K. Y. Wong, and K. K. Tsia, “Optical Time Stretch for High-Speed and High-Throughput Imaging — From Single-Cell to Tissue-Wide Scales,” IEEE J. Sel. Top. Quantum Electron. 22, 6803115 (2016).

A. K. Lau, H. C. Shum, K. K. Wong, and K. K. Tsia, “Optofluidic time-stretch imaging - an emerging tool for high-throughput imaging flow cytometry,” Lab Chip 16(10), 1743–1756 (2016).
[Crossref] [PubMed]

A. K. Lau, T. T. Wong, K. K. Ho, M. T. Tang, A. C. Chan, X. Wei, E. Y. Lam, H. C. Shum, K. K. Wong, and K. K. Tsia, “Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm,” J. Biomed. Opt. 19(7), 076001 (2014).
[Crossref] [PubMed]

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

K. K. Tsia, K. Goda, D. Capewell, and B. Jalali, “Performance of serial time-encoded amplified microscope,” Opt. Express 18(10), 10016–10028 (2010).
[Crossref] [PubMed]

K. Goda, K. K. Tsia, and B. Jalali, “Serial time-encoded amplified imaging for real-time observation of fast dynamic phenomena,” Nature 458(7242), 1145–1149 (2009).
[Crossref] [PubMed]

von Stetten, F.

O. Strohmeier, M. Keller, F. Schwemmer, S. Zehnle, D. Mark, F. von Stetten, R. Zengerle, and N. Paust, “Centrifugal microfluidic platforms: advanced unit operations and applications,” Chem. Soc. Rev. 44(17), 6187–6229 (2015).
[Crossref] [PubMed]

Walker, C.

H. Ramachandraiah, M. Amasia, J. Cole, P. Sheard, S. Pickhaver, C. Walker, V. Wirta, P. Lexow, R. Lione, and A. Russom, “Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics,” Lab Chip 13(8), 1578–1585 (2013).
[Crossref] [PubMed]

Walsh, J. R.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Wang, C.

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

Wang, C.-Y.

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

Wang, L.

L. Wang and P. C. Li, “Microfluidic DNA microarray analysis: a review,” Anal. Chim. Acta 687(1), 12–27 (2011).
[Crossref] [PubMed]

Wei, X.

A. K. Lau, T. T. Wong, K. K. Ho, M. T. Tang, A. C. Chan, X. Wei, E. Y. Lam, H. C. Shum, K. K. Wong, and K. K. Tsia, “Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm,” J. Biomed. Opt. 19(7), 076001 (2014).
[Crossref] [PubMed]

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

Werb, Z.

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

Wirta, V.

H. Ramachandraiah, M. Amasia, J. Cole, P. Sheard, S. Pickhaver, C. Walker, V. Wirta, P. Lexow, R. Lione, and A. Russom, “Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics,” Lab Chip 13(8), 1578–1585 (2013).
[Crossref] [PubMed]

Wobus, M.

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

Wong, K. K.

A. K. Lau, H. C. Shum, K. K. Wong, and K. K. Tsia, “Optofluidic time-stretch imaging - an emerging tool for high-throughput imaging flow cytometry,” Lab Chip 16(10), 1743–1756 (2016).
[Crossref] [PubMed]

A. K. Lau, T. T. Wong, K. K. Ho, M. T. Tang, A. C. Chan, X. Wei, E. Y. Lam, H. C. Shum, K. K. Wong, and K. K. Tsia, “Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm,” J. Biomed. Opt. 19(7), 076001 (2014).
[Crossref] [PubMed]

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

Wong, K. K. Y.

A. K. S. Lau, A. H. L. Tang, X. W. J. Xu, K. K. Y. Wong, and K. K. Tsia, “Optical Time Stretch for High-Speed and High-Throughput Imaging — From Single-Cell to Tissue-Wide Scales,” IEEE J. Sel. Top. Quantum Electron. 22, 6803115 (2016).

Wong, T. T.

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

A. K. Lau, T. T. Wong, K. K. Ho, M. T. Tang, A. C. Chan, X. Wei, E. Y. Lam, H. C. Shum, K. K. Wong, and K. K. Tsia, “Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm,” J. Biomed. Opt. 19(7), 076001 (2014).
[Crossref] [PubMed]

Xu, X. W. J.

A. K. S. Lau, A. H. L. Tang, X. W. J. Xu, K. K. Y. Wong, and K. K. Tsia, “Optical Time Stretch for High-Speed and High-Throughput Imaging — From Single-Cell to Tissue-Wide Scales,” IEEE J. Sel. Top. Quantum Electron. 22, 6803115 (2016).

Yaswen, P.

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

Yeow, K.

P. Lang, K. Yeow, A. Nichols, and A. Scheer, “Cellular imaging in drug discovery,” Nat. Rev. Drug Discov. 5(4), 343–356 (2006).
[Crossref] [PubMed]

Ying, Y.

H. T. Tse, D. R. Gossett, Y. S. Moon, M. Masaeli, M. Sohsman, Y. Ying, K. Mislick, R. P. Adams, J. Rao, and D. Di Carlo, “Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping,” Sci. Transl. Med. 5(212), 212ra163 (2013).
[Crossref] [PubMed]

Young, D. W.

Y. Feng, T. J. Mitchison, A. Bender, D. W. Young, and J. A. Tallarico, “Multi-parameter phenotypic profiling: using cellular effects to characterize small-molecule compounds,” Nat. Rev. Drug Discov. 8(7), 567–578 (2009).
[Crossref] [PubMed]

Yu, M.

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

Yu, Y.

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

Zalevsky, Z.

Zangle, T. A.

T. A. Zangle and M. A. Teitell, “Live-cell mass profiling: an emerging approach in quantitative biophysics,” Nat. Methods 11(12), 1221–1228 (2014).
[Crossref] [PubMed]

Zehnle, S.

O. Strohmeier, M. Keller, F. Schwemmer, S. Zehnle, D. Mark, F. von Stetten, R. Zengerle, and N. Paust, “Centrifugal microfluidic platforms: advanced unit operations and applications,” Chem. Soc. Rev. 44(17), 6187–6229 (2015).
[Crossref] [PubMed]

Zengerle, R.

O. Strohmeier, M. Keller, F. Schwemmer, S. Zehnle, D. Mark, F. von Stetten, R. Zengerle, and N. Paust, “Centrifugal microfluidic platforms: advanced unit operations and applications,” Chem. Soc. Rev. 44(17), 6187–6229 (2015).
[Crossref] [PubMed]

Zhou, A.

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

Anal. Chem. (1)

J. Park, V. Sunkara, T. H. Kim, H. Hwang, and Y. K. Cho, “Lab-on-a-disc for fully integrated multiplex immunoassays,” Anal. Chem. 84(5), 2133–2140 (2012).
[Crossref] [PubMed]

Anal. Chim. Acta (1)

L. Wang and P. C. Li, “Microfluidic DNA microarray analysis: a review,” Anal. Chim. Acta 687(1), 12–27 (2011).
[Crossref] [PubMed]

Appl. Phys. Rev. (1)

C. Lei, B. Guo, Z. Cheng, and K. Goda, “Optical time-stretch imaging: Principles and applications,” Appl. Phys. Rev. 3(1), 011102 (2016).
[Crossref]

Cell (1)

M. Boutros, F. Heigwer, and C. Laufer, “Microscopy-Based High-Content Screening,” Cell 163(6), 1314–1325 (2015).
[Crossref] [PubMed]

Chem. Soc. Rev. (1)

O. Strohmeier, M. Keller, F. Schwemmer, S. Zehnle, D. Mark, F. von Stetten, R. Zengerle, and N. Paust, “Centrifugal microfluidic platforms: advanced unit operations and applications,” Chem. Soc. Rev. 44(17), 6187–6229 (2015).
[Crossref] [PubMed]

Clin. Cancer Res. (1)

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

IEEE J. Sel. Top. Quantum Electron. (1)

A. K. S. Lau, A. H. L. Tang, X. W. J. Xu, K. K. Y. Wong, and K. K. Tsia, “Optical Time Stretch for High-Speed and High-Throughput Imaging — From Single-Cell to Tissue-Wide Scales,” IEEE J. Sel. Top. Quantum Electron. 22, 6803115 (2016).

J. Biomed. Opt. (1)

A. K. Lau, T. T. Wong, K. K. Ho, M. T. Tang, A. C. Chan, X. Wei, E. Y. Lam, H. C. Shum, K. K. Wong, and K. K. Tsia, “Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm,” J. Biomed. Opt. 19(7), 076001 (2014).
[Crossref] [PubMed]

J. Opt. Soc. Am. A (1)

Lab Chip (3)

H. Ramachandraiah, M. Amasia, J. Cole, P. Sheard, S. Pickhaver, C. Walker, V. Wirta, P. Lexow, R. Lione, and A. Russom, “Lab-on-DVD: standard DVD drives as a novel laser scanning microscope for image based point of care diagnostics,” Lab Chip 13(8), 1578–1585 (2013).
[Crossref] [PubMed]

R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. S. Lee, J. M. Park, J. Kim, H. Kim, M. Madou, and Y. K. Cho, “Centrifugal microfluidics for biomedical applications,” Lab Chip 10(14), 1758–1773 (2010).
[Crossref] [PubMed]

A. K. Lau, H. C. Shum, K. K. Wong, and K. K. Tsia, “Optofluidic time-stretch imaging - an emerging tool for high-throughput imaging flow cytometry,” Lab Chip 16(10), 1743–1756 (2016).
[Crossref] [PubMed]

Nat. Methods (2)

O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck, “Real-time deformability cytometry: on-the-fly cell mechanical phenotyping,” Nat. Methods 12(3), 199–202 (2015).
[Crossref] [PubMed]

T. A. Zangle and M. A. Teitell, “Live-cell mass profiling: an emerging approach in quantitative biophysics,” Nat. Methods 11(12), 1221–1228 (2014).
[Crossref] [PubMed]

Nat. Photonics (1)

K. Goda and B. Jalali, “Dispersive Fourier transformation for fast continuous single-shot measurements,” Nat. Photonics 7(2), 102 (2013).
[Crossref]

Nat. Rev. Cancer (1)

C. Alix-Panabières and K. Pantel, “Challenges in circulating tumour cell research,” Nat. Rev. Cancer 14(9), 623–631 (2014).
[Crossref] [PubMed]

Nat. Rev. Drug Discov. (2)

Y. Feng, T. J. Mitchison, A. Bender, D. W. Young, and J. A. Tallarico, “Multi-parameter phenotypic profiling: using cellular effects to characterize small-molecule compounds,” Nat. Rev. Drug Discov. 8(7), 567–578 (2009).
[Crossref] [PubMed]

P. Lang, K. Yeow, A. Nichols, and A. Scheer, “Cellular imaging in drug discovery,” Nat. Rev. Drug Discov. 5(4), 343–356 (2006).
[Crossref] [PubMed]

Nature (2)

K. Goda, K. K. Tsia, and B. Jalali, “Serial time-encoded amplified imaging for real-time observation of fast dynamic phenomena,” Nature 458(7242), 1145–1149 (2009).
[Crossref] [PubMed]

D. A. Lawson, N. R. Bhakta, K. Kessenbrock, K. D. Prummel, Y. Yu, K. Takai, A. Zhou, H. Eyob, S. Balakrishnan, C.-Y. Wang, P. Yaswen, A. Goga, and Z. Werb, “Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells,” Nature 526(7571), 131–135 (2015).
[Crossref] [PubMed]

Opt. Express (1)

Proc. Natl. Acad. Sci. U.S.A. (1)

K. Goda, A. Ayazi, D. R. Gossett, J. Sadasivam, C. K. Lonappan, E. Sollier, A. M. Fard, S. C. Hur, J. Adam, C. Murray, C. Wang, N. Brackbill, D. Di Carlo, and B. Jalali, “High-throughput single-microparticle imaging flow analyzer,” Proc. Natl. Acad. Sci. U.S.A. 109(29), 11630–11635 (2012).
[Crossref] [PubMed]

Sci. Rep. (2)

C. L. Chen, A. Mahjoubfar, L. C. Tai, I. K. Blaby, A. Huang, K. R. Niazi, and B. Jalali, “Deep Learning in Label-free Cell Classification,” Sci. Rep. 6, 21471 (2016).
[Crossref] [PubMed]

T. T. Wong, A. K. Lau, K. K. Ho, M. Y. Tang, J. D. Robles, X. Wei, A. C. Chan, A. H. Tang, E. Y. Lam, K. K. Wong, G. C. Chan, H. C. Shum, and K. K. Tsia, “Asymmetric-detection time-stretch optical microscopy (ATOM) for ultrafast high-contrast cellular imaging in flow,” Sci. Rep. 4, 3656 (2014).
[Crossref] [PubMed]

Sci. Transl. Med. (2)

E. Ozkumur, A. M. Shah, J. C. Ciciliano, B. L. Emmink, D. T. Miyamoto, E. Brachtel, M. Yu, P. I. Chen, B. Morgan, J. Trautwein, A. Kimura, S. Sengupta, S. L. Stott, N. M. Karabacak, T. A. Barber, J. R. Walsh, K. Smith, P. S. Spuhler, J. P. Sullivan, R. J. Lee, D. T. Ting, X. Luo, A. T. Shaw, A. Bardia, L. V. Sequist, D. N. Louis, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Inertial focusing for tumor antigen-dependent and -independent sorting of rare circulating tumor cells,” Sci. Transl. Med. 5(179), 179ra47 (2013).
[Crossref] [PubMed]

H. T. Tse, D. R. Gossett, Y. S. Moon, M. Masaeli, M. Sohsman, Y. Ying, K. Mislick, R. P. Adams, J. Rao, and D. Di Carlo, “Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping,” Sci. Transl. Med. 5(212), 212ra163 (2013).
[Crossref] [PubMed]

Other (2)

J. Wu, Y. Xu, J. Xu, X. Wei, A. C. Chan, A. H. Tang, A. K. Lau, B. M. Chung, H. C. Shum, E. Y. Lam, K. K. Wong and K. K. Tsia, Ultrafast Laser-Scanning Time-Stretch Imaging at Visible Wavelengths. Light: Science & Applications accepted article preview 12 August 2016.

I. A. V. Natasha S. Barteneva, Imaging Flow Cytometry: Methods and Protocols, 1st ed., Methods in Molecular Biology (Humana Press, 2016).

Cited By

OSA participates in Crossref's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (5)

Fig. 1
Fig. 1

(a) A schematic of the time-stretch imaging system. Laser pulses (from a fiber mode-locked laser) are first time-stretched within a dispersive fiber and thus form wavelength-swept waveforms. A holographic diffraction grating together the relay lenses (L1 and L2) and the objective lens (Obj1) is used to transform the wavelength-swept beam into 1D spectrally-encoded line-scan beam, which is projected onto the modified spinning DVD substrate. The encoded line-scan beam is returned along the same path, by placing a mirror at the entrance pupil of the back objective lens (Obj2), forming a double-pass configuration. Upon recombined back to the Gaussian beam profile, the imaged-encoded beam is eventually detected by a high-speed photo-receiver (bandwidth of 12GHz) and recorded by a high-speed real-time oscilloscope (bandwidth of 16GHz; sampling rate of 80GSa/s). (b) A design schematic of the modified DVD assay platform employed in this work (Four assay wells/sites are depicted in (b)). The substrate is composed of two polycarbonate layers (See the cross-sectional view), obtained from two separate DVDs, that are bonded together with UV-cured adhesive. This creates assay chambers with a height of ~100-120 µm (defined by the spacers). The spacers are also carefully aligned to stabilize the rapid spinning motion. (c) A photo of a spinning DVD mounted on a modified DVD drive.

Fig. 2
Fig. 2

(a) Time-stretch image of MCF-7 (0.42 mm x 34 mm) cultured on the modified DVD captured at a single-shot line-scan of 11 MHz. The spinning speed is 900 rpm (linear speed of ~4 m/s). (b) zoom-in view of the area highlighted in (a). (c) (left column) further zoom-in view of the areas highlighted in (b). (Right column) Phase-contrast images of the same areas taken by commercial light microscope (Nikon Eclipse Ni-U). Arrows indicates the key cellular features identified in both time-stretch images and light microscope images. (c) Zoom-in view of a MCF-7 cell highlighted as the black box in (a). (d,e) The line-cuts along the fast axis (i.e. radial direction) and the slow axis (i.e. circumferential direction) of the selected cell in (c). The two line-cut plots clearly show that the ability of the system to resolve sub-cellular structure with a feature size better than 2.4 µm, consistent with the theoretical resolution of ~2 µm (see the text for detailed discussion). All scale bars (in red) represent 50 µm.

Fig. 3
Fig. 3

(a) Modified DVD assay design for time-stretch imaging of specifically-captured biotinated-polystyrene microparticles. The single-layer substrate in this experiment adopted a four-well design: two are target wells coated with streptavidin for specific capture of microparticles (marked as T) whereas another two are control wells in which no streptavidin coating is present (marked as C). The curve line on the DVD indicates the recording area. (b) (Left column) Time-stretch images of captured microparticles in wells T and C taken under a spinning speed of 3000 rpm (speed of 14 m/s). (Right column) Static images of the wells T and C taken by ordinary light microscope. (c) Statistical size distribution of the captured 4657 microparticles analysed from the time-stretch image (Red curve is the Gaussian fit). All scale bars (in red) represent 50 µm.

Fig. 4
Fig. 4

(a) Modified DVD assay design for time-stretch imaging of specifically-captured MCF-7. The substrate adopted an eight-well design: four are target wells coated with anti-EpCAM antibody (marked as T) whereas the other four are control wells with only streptavidin coating (marked as C). A schematic of the antibody-captured MCF-7 in the target wells is also shown. The curve line on the DVD indicates the recording area. (b) (Left) Time-stretch image of antibody-captured MCF-7 cells in the target well on the spinning DVD (at a spinning speed of 2400 rpm and a linear speed of 11 m/s). (Middle) Zoom-in views of the time-stretch image are also shown. (Right) Time-stretch image of control wells under the same spinning condition. (c) The analysis of the cell-capture specificity in the experiments. The percentages of specificity and non-specificity are calculated from the number of remaining cells upon rinsing out of the number of MCF-7 captured in the antibody-coated and streptavidin-coated wells respectively. (d) Static images of the captured cells (phase-contrast (left) and fluorescence (right)) treated with vital staining (propidium iodide) on the DVD substrate, taken after (top) 2-minutes and (bottom) 32-minute of spinning. All scale bars (in red) represent 50 µm.

Fig. 5
Fig. 5

(a) (Left) Specimen of MCF-7 (25%) mixed with human buffy coat (75%) used for antibody-captured, and thus enrichment of, MCF-7. (Middle) Time-stretch image of the enriched MCF-7 (with anti-EpCAM antibody) in the target well. (Right) Time-stretch image of the control well in which only streptavidin is coated. Both the images in (a) were taken at a spinning speed of 900 rpm (linear speed of 4 m/s). Same substrate design as shown in Fig. 4. was adopted in this experiment. (b) Static images (Top: phase contrast; Bottom: fluorescence) of the enriched MCF-7 further stained with green fluorescent dye (Alexa Fluor-488 anti-EpCAM (RnD FAB9601G, 10 µg/mL)). This additional staining step was performed to further confirm the MCF-7 enriched on the DVD. All scale bars (in red) represent 50 µm.

Metrics