Abstract

A wide-field fluorescence microscope with a double-helix point spread function (PSF) is constructed to obtain the specimen’s three-dimensional distribution with a single snapshot. Spiral-phase-based computer-generated holograms (CGHs) are adopted to make the depth-of-field of the microscope adjustable. The impact of system aberrations on the double-helix PSF at high numerical aperture is analyzed to reveal the necessity of the aberration correction. A modified cepstrum-based reconstruction scheme is promoted in accordance with properties of the new double-helix PSF. The extended depth-of-field images and the corresponding depth maps for both a simulated sample and a tilted section slice of bovine pulmonary artery endothelial (BPAE) cells are recovered, respectively, verifying that the depth-of-field is properly extended and the depth of the specimen can be estimated at a precision of 23.4nm. This three-dimensional fluorescence microscope with a framerate-rank time resolution is suitable for studying the fast developing process of thin and sparsely distributed micron-scale cells in extended depth-of-field.

© 2017 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Full Article  |  PDF Article
OSA Recommended Articles
Single shot three-dimensional imaging using an engineered point spread function

René Berlich, Andreas Bräuer, and Sjoerd Stallinga
Opt. Express 24(6) 5946-5960 (2016)

Nanoscale three-dimensional single particle tracking by light-sheet-based double-helix point spread function microscopy

Bin Yu, Jie Yu, Weihai Li, Bo Cao, Heng Li, Danni Chen, and Hanben Niu
Appl. Opt. 55(3) 449-453 (2016)

Fast and accurate three-dimensional point spread function computation for fluorescence microscopy

Jizhou Li, Feng Xue, and Thierry Blu
J. Opt. Soc. Am. A 34(6) 1029-1034 (2017)

References

  • View by:
  • |
  • |
  • |

  1. H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
    [PubMed]
  2. W. R. Legant, L. Shao, J. B. Grimm, T. A. Brown, D. E. Milkie, B. B. Avants, L. D. Lavis, and E. Betzig, “High-density three-dimensional localization microscopy across large volumes,” Nat. Methods 13(4), 359–365 (2016).
    [PubMed]
  3. P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7(8), 637–642 (2010).
    [PubMed]
  4. B. L. Scott and A. D. Hoppe, “Three-dimensional reconstruction of three-way fret microscopy improves imaging of multiple protein-protein interactions,” PLoS One 11(3), e0152401 (2016).
    [PubMed]
  5. S. K. Nayar and Y. Nakagawa, “Shape from focus,” IEEE Trans. Pattern Anal. Mach. Intell. 16(8), 824–831 (1994).
  6. X. Lin, J. Suo, G. Wetzstein, Q. Dai, and R. Raskar, “Coded focal stack photography,” in Computational Photography (ICCP),2013IEEE International Conference on, (IEEE, 2013), 1–9.
  7. T. Wilson, “Optical sectioning in confocal fluorescence microscopes,” J. Microsc. 154, 143–156 (1989).
  8. H. J. Jeong, H. Yoo, and D. Gweon, “High-speed 3-D measurement with a large field of view based on direct-view confocal microscope with an electrically tunable lens,” Opt. Express 24(4), 3806–3816 (2016).
    [PubMed]
  9. D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
    [PubMed]
  10. M. A. Neil, R. Juškaitis, and T. Wilson, “Method of obtaining optical sectioning by using structured light in a conventional microscope,” Opt. Lett. 22(24), 1905–1907 (1997).
    [PubMed]
  11. X. Zhou, M. Lei, D. Dan, B. Yao, J. Qian, S. Yan, Y. Yang, J. Min, T. Peng, T. Ye, and G. Chen, “Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction,” PLoS One 10(3), e0120892 (2015).
    [PubMed]
  12. B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
    [PubMed]
  13. E. R. Dowski and W. T. Cathey, “Extended depth of field through wave-front coding,” Appl. Opt. 34(11), 1859–1866 (1995).
    [PubMed]
  14. M. Demenikov and A. R. Harvey, “Image artifacts in hybrid imaging systems with a cubic phase mask,” Opt. Express 18(8), 8207–8212 (2010).
    [PubMed]
  15. J. A. Conchello and M. E. Dresser, “Extended depth-of-focus microscopy via constrained deconvolution,” J. Biomed. Opt. 12(6), 064026 (2007).
    [PubMed]
  16. S. Kim, S. Jun, E. Lee, J. Shin, and J. Paik, “Real-time bayer-domain image restoration for an extended depth of field (EDoF) camera,” IEEE Trans. Consum. Electron. 55(4), 1756–1764 (2009).
  17. P. Zammit, A. R. Harvey, and G. Carles, “Extended depth-of-field imaging and ranging in a snapshot,” Optica 1(4), 209–216 (2014).
  18. P. Zammit, A. R. Harvey, and G. Carles, “Practical single snapshot 3D imaging method with an extended depth of field,” in Computational Optical Sensing and Imaging, (Optical Society of America, 2015), CT2E. 2.
  19. R. Berlich, A. Bräuer, and S. Stallinga, “Single shot three-dimensional imaging using an engineered point spread function,” Opt. Express 24(6), 5946–5960 (2016).
    [PubMed]
  20. A. Greengard, Y. Y. Schechner, and R. Piestun, “Depth from diffracted rotation,” Opt. Lett. 31(2), 181–183 (2006).
    [PubMed]
  21. F. Zhou, R. Ye, G. Li, H. Zhang, and D. Wang, “Optimized circularly symmetric phase mask to extend the depth of focus,” J. Opt. Soc. Am. A 26(8), 1889–1895 (2009).
    [PubMed]
  22. Z. Fan and Q. D. Duong, “To extend the depth of field by using the asymmetrical phase mask and its conjugation phase mask in wavefront coding imaging systems,” Appl. Opt. 54(12), 3630–3634 (2015).
  23. K. Chu, N. George, and W. Chi, “Extending the depth of field through unbalanced optical path difference,” Appl. Opt. 47(36), 6895–6903 (2008).
    [PubMed]
  24. S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
    [PubMed]
  25. D. Chen, B. Yu, H. Li, Y. Huo, B. Cao, G. Xu, and H. Niu, “Approach to multiparticle parallel tracking in thick samples with three-dimensional nanoresolution,” Opt. Lett. 38(19), 3712–3715 (2013).
    [PubMed]
  26. S. R. P. Pavani and R. Piestun, “High-efficiency rotating point spread functions,” Opt. Express 16(5), 3484–3489 (2008).
    [PubMed]
  27. R. Piestun and J. Shamir, “Generalized propagation-invariant wave fields,” J. Opt. Soc. Am. A 15(12), 3039–3044 (1998).
  28. S. Prasad, “Rotating point spread function via pupil-phase engineering,” Opt. Lett. 38(4), 585–587 (2013).
    [PubMed]
  29. M. Baránek and Z. Bouchal, “Optimizing the rotating point spread function by SLM aided spiral phase modulation,” in XIX Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics, (International Society for Optics and Photonics, 2014), 94410N.
  30. S. Ghosh, G. Grover, R. Piestun, and C. Preza, “Effect of double-helix point-spread functions on 3D imaging in the presence of spherical aberrations, ” in SPIE BiOS, (International Society for Optics and Photonics, 2011), 79041D–79041D–79049.
  31. S. Ghosh and C. Preza, “Characterization of a three-dimensional double-helix point-spread function for fluorescence microscopy in the presence of spherical aberration,” J. Biomed. Opt. 18(3), 036010 (2013).
    [PubMed]
  32. A. Jesacher, A. Schwaighofer, S. Fürhapter, C. Maurer, S. Bernet, and M. Ritsch-Marte, “Wavefront correction of spatial light modulators using an optical vortex image,” Opt. Express 15(9), 5801–5808 (2007).
    [PubMed]

2016 (4)

W. R. Legant, L. Shao, J. B. Grimm, T. A. Brown, D. E. Milkie, B. B. Avants, L. D. Lavis, and E. Betzig, “High-density three-dimensional localization microscopy across large volumes,” Nat. Methods 13(4), 359–365 (2016).
[PubMed]

B. L. Scott and A. D. Hoppe, “Three-dimensional reconstruction of three-way fret microscopy improves imaging of multiple protein-protein interactions,” PLoS One 11(3), e0152401 (2016).
[PubMed]

H. J. Jeong, H. Yoo, and D. Gweon, “High-speed 3-D measurement with a large field of view based on direct-view confocal microscope with an electrically tunable lens,” Opt. Express 24(4), 3806–3816 (2016).
[PubMed]

R. Berlich, A. Bräuer, and S. Stallinga, “Single shot three-dimensional imaging using an engineered point spread function,” Opt. Express 24(6), 5946–5960 (2016).
[PubMed]

2015 (2)

Z. Fan and Q. D. Duong, “To extend the depth of field by using the asymmetrical phase mask and its conjugation phase mask in wavefront coding imaging systems,” Appl. Opt. 54(12), 3630–3634 (2015).

X. Zhou, M. Lei, D. Dan, B. Yao, J. Qian, S. Yan, Y. Yang, J. Min, T. Peng, T. Ye, and G. Chen, “Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction,” PLoS One 10(3), e0120892 (2015).
[PubMed]

2014 (2)

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

P. Zammit, A. R. Harvey, and G. Carles, “Extended depth-of-field imaging and ranging in a snapshot,” Optica 1(4), 209–216 (2014).

2013 (4)

S. Prasad, “Rotating point spread function via pupil-phase engineering,” Opt. Lett. 38(4), 585–587 (2013).
[PubMed]

D. Chen, B. Yu, H. Li, Y. Huo, B. Cao, G. Xu, and H. Niu, “Approach to multiparticle parallel tracking in thick samples with three-dimensional nanoresolution,” Opt. Lett. 38(19), 3712–3715 (2013).
[PubMed]

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

S. Ghosh and C. Preza, “Characterization of a three-dimensional double-helix point-spread function for fluorescence microscopy in the presence of spherical aberration,” J. Biomed. Opt. 18(3), 036010 (2013).
[PubMed]

2010 (2)

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7(8), 637–642 (2010).
[PubMed]

M. Demenikov and A. R. Harvey, “Image artifacts in hybrid imaging systems with a cubic phase mask,” Opt. Express 18(8), 8207–8212 (2010).
[PubMed]

2009 (3)

F. Zhou, R. Ye, G. Li, H. Zhang, and D. Wang, “Optimized circularly symmetric phase mask to extend the depth of focus,” J. Opt. Soc. Am. A 26(8), 1889–1895 (2009).
[PubMed]

S. Kim, S. Jun, E. Lee, J. Shin, and J. Paik, “Real-time bayer-domain image restoration for an extended depth of field (EDoF) camera,” IEEE Trans. Consum. Electron. 55(4), 1756–1764 (2009).

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[PubMed]

2008 (2)

2007 (3)

H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[PubMed]

J. A. Conchello and M. E. Dresser, “Extended depth-of-focus microscopy via constrained deconvolution,” J. Biomed. Opt. 12(6), 064026 (2007).
[PubMed]

A. Jesacher, A. Schwaighofer, S. Fürhapter, C. Maurer, S. Bernet, and M. Ritsch-Marte, “Wavefront correction of spatial light modulators using an optical vortex image,” Opt. Express 15(9), 5801–5808 (2007).
[PubMed]

2006 (1)

1998 (1)

1997 (1)

1995 (1)

1994 (1)

S. K. Nayar and Y. Nakagawa, “Shape from focus,” IEEE Trans. Pattern Anal. Mach. Intell. 16(8), 824–831 (1994).

1989 (1)

T. Wilson, “Optical sectioning in confocal fluorescence microscopes,” J. Microsc. 154, 143–156 (1989).

Avants, B. B.

W. R. Legant, L. Shao, J. B. Grimm, T. A. Brown, D. E. Milkie, B. B. Avants, L. D. Lavis, and E. Betzig, “High-density three-dimensional localization microscopy across large volumes,” Nat. Methods 13(4), 359–365 (2016).
[PubMed]

Bao, Z.

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7(8), 637–642 (2010).
[PubMed]

Becker, K.

H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[PubMed]

Bembenek, J. N.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Berlich, R.

Bernet, S.

Betzig, E.

W. R. Legant, L. Shao, J. B. Grimm, T. A. Brown, D. E. Milkie, B. B. Avants, L. D. Lavis, and E. Betzig, “High-density three-dimensional localization microscopy across large volumes,” Nat. Methods 13(4), 359–365 (2016).
[PubMed]

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Biteen, J. S.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[PubMed]

Böhme, R.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Bräuer, A.

Brown, T. A.

W. R. Legant, L. Shao, J. B. Grimm, T. A. Brown, D. E. Milkie, B. B. Avants, L. D. Lavis, and E. Betzig, “High-density three-dimensional localization microscopy across large volumes,” Nat. Methods 13(4), 359–365 (2016).
[PubMed]

Cao, B.

Carles, G.

Cathey, W. T.

Chen, B. C.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Chen, D.

Chen, G.

X. Zhou, M. Lei, D. Dan, B. Yao, J. Qian, S. Yan, Y. Yang, J. Min, T. Peng, T. Ye, and G. Chen, “Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction,” PLoS One 10(3), e0120892 (2015).
[PubMed]

Chi, W.

Chu, K.

Conchello, J. A.

J. A. Conchello and M. E. Dresser, “Extended depth-of-focus microscopy via constrained deconvolution,” J. Biomed. Opt. 12(6), 064026 (2007).
[PubMed]

Dan, D.

X. Zhou, M. Lei, D. Dan, B. Yao, J. Qian, S. Yan, Y. Yang, J. Min, T. Peng, T. Ye, and G. Chen, “Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction,” PLoS One 10(3), e0120892 (2015).
[PubMed]

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

Davidson, M. W.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Deininger, K.

H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[PubMed]

Demenikov, M.

Deussing, J. M.

H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[PubMed]

Dodt, H.-U.

H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[PubMed]

Dowski, E. R.

Dresser, M. E.

J. A. Conchello and M. E. Dresser, “Extended depth-of-focus microscopy via constrained deconvolution,” J. Biomed. Opt. 12(6), 064026 (2007).
[PubMed]

Duong, Q. D.

Eder, M.

H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[PubMed]

English, B. P.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Fan, Z.

Fritz-Laylin, L.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Fürhapter, S.

Gao, P.

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

George, N.

Ghosh, S.

S. Ghosh and C. Preza, “Characterization of a three-dimensional double-helix point-spread function for fluorescence microscopy in the presence of spherical aberration,” J. Biomed. Opt. 18(3), 036010 (2013).
[PubMed]

Greengard, A.

Grill, S. W.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Grimm, J. B.

W. R. Legant, L. Shao, J. B. Grimm, T. A. Brown, D. E. Milkie, B. B. Avants, L. D. Lavis, and E. Betzig, “High-density three-dimensional localization microscopy across large volumes,” Nat. Methods 13(4), 359–365 (2016).
[PubMed]

Gweon, D.

Hammer, J. A.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Harvey, A. R.

Hoppe, A. D.

B. L. Scott and A. D. Hoppe, “Three-dimensional reconstruction of three-way fret microscopy improves imaging of multiple protein-protein interactions,” PLoS One 11(3), e0152401 (2016).
[PubMed]

Huo, Y.

Jährling, N.

H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[PubMed]

Janetopoulos, C.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Jeong, H. J.

Jesacher, A.

Jun, S.

S. Kim, S. Jun, E. Lee, J. Shin, and J. Paik, “Real-time bayer-domain image restoration for an extended depth of field (EDoF) camera,” IEEE Trans. Consum. Electron. 55(4), 1756–1764 (2009).

Juškaitis, R.

Keller, P. J.

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7(8), 637–642 (2010).
[PubMed]

Khairy, K.

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7(8), 637–642 (2010).
[PubMed]

Kiehart, D. P.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Kim, S.

S. Kim, S. Jun, E. Lee, J. Shin, and J. Paik, “Real-time bayer-domain image restoration for an extended depth of field (EDoF) camera,” IEEE Trans. Consum. Electron. 55(4), 1756–1764 (2009).

Lavis, L. D.

W. R. Legant, L. Shao, J. B. Grimm, T. A. Brown, D. E. Milkie, B. B. Avants, L. D. Lavis, and E. Betzig, “High-density three-dimensional localization microscopy across large volumes,” Nat. Methods 13(4), 359–365 (2016).
[PubMed]

Lee, E.

S. Kim, S. Jun, E. Lee, J. Shin, and J. Paik, “Real-time bayer-domain image restoration for an extended depth of field (EDoF) camera,” IEEE Trans. Consum. Electron. 55(4), 1756–1764 (2009).

Legant, W. R.

W. R. Legant, L. Shao, J. B. Grimm, T. A. Brown, D. E. Milkie, B. B. Avants, L. D. Lavis, and E. Betzig, “High-density three-dimensional localization microscopy across large volumes,” Nat. Methods 13(4), 359–365 (2016).
[PubMed]

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Lei, M.

X. Zhou, M. Lei, D. Dan, B. Yao, J. Qian, S. Yan, Y. Yang, J. Min, T. Peng, T. Ye, and G. Chen, “Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction,” PLoS One 10(3), e0120892 (2015).
[PubMed]

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

Leischner, U.

H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[PubMed]

Li, G.

Li, H.

Lippincott-Schwartz, J.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Liu, N.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[PubMed]

Liu, Z.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Lord, S. J.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[PubMed]

Mauch, C. P.

H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[PubMed]

Maurer, C.

Milkie, D. E.

W. R. Legant, L. Shao, J. B. Grimm, T. A. Brown, D. E. Milkie, B. B. Avants, L. D. Lavis, and E. Betzig, “High-density three-dimensional localization microscopy across large volumes,” Nat. Methods 13(4), 359–365 (2016).
[PubMed]

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Mimori-Kiyosue, Y.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Min, J.

X. Zhou, M. Lei, D. Dan, B. Yao, J. Qian, S. Yan, Y. Yang, J. Min, T. Peng, T. Ye, and G. Chen, “Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction,” PLoS One 10(3), e0120892 (2015).
[PubMed]

Mitchell, D. M.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Moerner, W. E.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[PubMed]

Mullins, R. D.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Nakagawa, Y.

S. K. Nayar and Y. Nakagawa, “Shape from focus,” IEEE Trans. Pattern Anal. Mach. Intell. 16(8), 824–831 (1994).

Nayar, S. K.

S. K. Nayar and Y. Nakagawa, “Shape from focus,” IEEE Trans. Pattern Anal. Mach. Intell. 16(8), 824–831 (1994).

Neil, M. A.

Niu, H.

Paik, J.

S. Kim, S. Jun, E. Lee, J. Shin, and J. Paik, “Real-time bayer-domain image restoration for an extended depth of field (EDoF) camera,” IEEE Trans. Consum. Electron. 55(4), 1756–1764 (2009).

Pavani, S. R. P.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[PubMed]

S. R. P. Pavani and R. Piestun, “High-efficiency rotating point spread functions,” Opt. Express 16(5), 3484–3489 (2008).
[PubMed]

Peng, T.

X. Zhou, M. Lei, D. Dan, B. Yao, J. Qian, S. Yan, Y. Yang, J. Min, T. Peng, T. Ye, and G. Chen, “Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction,” PLoS One 10(3), e0120892 (2015).
[PubMed]

Piestun, R.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[PubMed]

S. R. P. Pavani and R. Piestun, “High-efficiency rotating point spread functions,” Opt. Express 16(5), 3484–3489 (2008).
[PubMed]

A. Greengard, Y. Y. Schechner, and R. Piestun, “Depth from diffracted rotation,” Opt. Lett. 31(2), 181–183 (2006).
[PubMed]

R. Piestun and J. Shamir, “Generalized propagation-invariant wave fields,” J. Opt. Soc. Am. A 15(12), 3039–3044 (1998).

Prasad, S.

Preza, C.

S. Ghosh and C. Preza, “Characterization of a three-dimensional double-helix point-spread function for fluorescence microscopy in the presence of spherical aberration,” J. Biomed. Opt. 18(3), 036010 (2013).
[PubMed]

Qi, Y.

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

Qian, J.

X. Zhou, M. Lei, D. Dan, B. Yao, J. Qian, S. Yan, Y. Yang, J. Min, T. Peng, T. Ye, and G. Chen, “Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction,” PLoS One 10(3), e0120892 (2015).
[PubMed]

Reymann, A. C.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Ritsch-Marte, M.

Ritter, A. T.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Romero, D. P.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Santella, A.

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7(8), 637–642 (2010).
[PubMed]

Schechner, Y. Y.

Schierloh, A.

H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[PubMed]

Schmidt, A. D.

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7(8), 637–642 (2010).
[PubMed]

Schwaighofer, A.

Scott, B. L.

B. L. Scott and A. D. Hoppe, “Three-dimensional reconstruction of three-way fret microscopy improves imaging of multiple protein-protein interactions,” PLoS One 11(3), e0152401 (2016).
[PubMed]

Seydoux, G.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Shamir, J.

Shao, L.

W. R. Legant, L. Shao, J. B. Grimm, T. A. Brown, D. E. Milkie, B. B. Avants, L. D. Lavis, and E. Betzig, “High-density three-dimensional localization microscopy across large volumes,” Nat. Methods 13(4), 359–365 (2016).
[PubMed]

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Shin, J.

S. Kim, S. Jun, E. Lee, J. Shin, and J. Paik, “Real-time bayer-domain image restoration for an extended depth of field (EDoF) camera,” IEEE Trans. Consum. Electron. 55(4), 1756–1764 (2009).

Stallinga, S.

Stelzer, E. H.

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7(8), 637–642 (2010).
[PubMed]

Thompson, M. A.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[PubMed]

Tulu, U. S.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Twieg, R. J.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[PubMed]

Wang, D.

Wang, J. T.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Wang, K.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Wang, W.

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

Wilson, T.

Winterhalder, M.

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

Wittbrodt, J.

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7(8), 637–642 (2010).
[PubMed]

Wu, X. S.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Xia, L.

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

Xu, G.

Yan, S.

X. Zhou, M. Lei, D. Dan, B. Yao, J. Qian, S. Yan, Y. Yang, J. Min, T. Peng, T. Ye, and G. Chen, “Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction,” PLoS One 10(3), e0120892 (2015).
[PubMed]

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

Yang, Y.

X. Zhou, M. Lei, D. Dan, B. Yao, J. Qian, S. Yan, Y. Yang, J. Min, T. Peng, T. Ye, and G. Chen, “Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction,” PLoS One 10(3), e0120892 (2015).
[PubMed]

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

Yao, B.

X. Zhou, M. Lei, D. Dan, B. Yao, J. Qian, S. Yan, Y. Yang, J. Min, T. Peng, T. Ye, and G. Chen, “Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction,” PLoS One 10(3), e0120892 (2015).
[PubMed]

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

Ye, R.

Ye, T.

X. Zhou, M. Lei, D. Dan, B. Yao, J. Qian, S. Yan, Y. Yang, J. Min, T. Peng, T. Ye, and G. Chen, “Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction,” PLoS One 10(3), e0120892 (2015).
[PubMed]

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

Yoo, H.

Yu, B.

Zammit, P.

Zhang, H.

Zhao, W.

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

Zhou, F.

Zhou, X.

X. Zhou, M. Lei, D. Dan, B. Yao, J. Qian, S. Yan, Y. Yang, J. Min, T. Peng, T. Ye, and G. Chen, “Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction,” PLoS One 10(3), e0120892 (2015).
[PubMed]

Zieglgänsberger, W.

H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[PubMed]

Zumbusch, A.

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

Appl. Opt. (3)

IEEE Trans. Consum. Electron. (1)

S. Kim, S. Jun, E. Lee, J. Shin, and J. Paik, “Real-time bayer-domain image restoration for an extended depth of field (EDoF) camera,” IEEE Trans. Consum. Electron. 55(4), 1756–1764 (2009).

IEEE Trans. Pattern Anal. Mach. Intell. (1)

S. K. Nayar and Y. Nakagawa, “Shape from focus,” IEEE Trans. Pattern Anal. Mach. Intell. 16(8), 824–831 (1994).

J. Biomed. Opt. (2)

J. A. Conchello and M. E. Dresser, “Extended depth-of-focus microscopy via constrained deconvolution,” J. Biomed. Opt. 12(6), 064026 (2007).
[PubMed]

S. Ghosh and C. Preza, “Characterization of a three-dimensional double-helix point-spread function for fluorescence microscopy in the presence of spherical aberration,” J. Biomed. Opt. 18(3), 036010 (2013).
[PubMed]

J. Microsc. (1)

T. Wilson, “Optical sectioning in confocal fluorescence microscopes,” J. Microsc. 154, 143–156 (1989).

J. Opt. Soc. Am. A (2)

Nat. Methods (3)

H.-U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[PubMed]

W. R. Legant, L. Shao, J. B. Grimm, T. A. Brown, D. E. Milkie, B. B. Avants, L. D. Lavis, and E. Betzig, “High-density three-dimensional localization microscopy across large volumes,” Nat. Methods 13(4), 359–365 (2016).
[PubMed]

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7(8), 637–642 (2010).
[PubMed]

Opt. Express (5)

Opt. Lett. (4)

Optica (1)

PLoS One (2)

B. L. Scott and A. D. Hoppe, “Three-dimensional reconstruction of three-way fret microscopy improves imaging of multiple protein-protein interactions,” PLoS One 11(3), e0152401 (2016).
[PubMed]

X. Zhou, M. Lei, D. Dan, B. Yao, J. Qian, S. Yan, Y. Yang, J. Min, T. Peng, T. Ye, and G. Chen, “Double-exposure optical sectioning structured illumination microscopy based on Hilbert transform reconstruction,” PLoS One 10(3), e0120892 (2015).
[PubMed]

Proc. Natl. Acad. Sci. U.S.A. (1)

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. U.S.A. 106(9), 2995–2999 (2009).
[PubMed]

Sci. Rep. (1)

D. Dan, M. Lei, B. Yao, W. Wang, M. Winterhalder, A. Zumbusch, Y. Qi, L. Xia, S. Yan, Y. Yang, P. Gao, T. Ye, and W. Zhao, “DMD-based LED-illumination super-resolution and optical sectioning microscopy,” Sci. Rep. 3, 1116 (2013).
[PubMed]

Science (1)

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[PubMed]

Other (4)

X. Lin, J. Suo, G. Wetzstein, Q. Dai, and R. Raskar, “Coded focal stack photography,” in Computational Photography (ICCP),2013IEEE International Conference on, (IEEE, 2013), 1–9.

P. Zammit, A. R. Harvey, and G. Carles, “Practical single snapshot 3D imaging method with an extended depth of field,” in Computational Optical Sensing and Imaging, (Optical Society of America, 2015), CT2E. 2.

M. Baránek and Z. Bouchal, “Optimizing the rotating point spread function by SLM aided spiral phase modulation,” in XIX Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics, (International Society for Optics and Photonics, 2014), 94410N.

S. Ghosh, G. Grover, R. Piestun, and C. Preza, “Effect of double-helix point-spread functions on 3D imaging in the presence of spherical aberrations, ” in SPIE BiOS, (International Society for Optics and Photonics, 2011), 79041D–79041D–79049.

Cited By

OSA participates in Crossref's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (9)

Fig. 1
Fig. 1

Schematic of the single shot, three-dimensional fluorescence microscope. The expanded and collimated laser beam is projected in parallel onto the specimen as an epi-illumination source. The wavefront of the fluorescence beam emitted from the specimen is modulated by the spatial light modulator, which is later focused onto the CCD camera. The inset shows an example of the loaded CGH on the spatial light modulator. DM: longpass dichroic mirror (λc = 500nm), SLM: spatial light modulator, M1-M8: mirrors.

Fig. 2
Fig. 2

The effects of CGH type on the double-helix PSF. Properties of the double-helix PSF generated by the GL-mode-based CGH (GL CGH) and the spiral-phase-based CGHs (SP CGH) for NA = 1.25, λ = 515nm. (a) Intensity distribution with varying amounts of defocus. (b) and (c) Variation of the inter-lobe distance and the rotation angle with varying amounts of defocus. (d) Radial and angular FWHM of the main lobes for the spiral-phase-based double-helix PSFs with different number of Fresnel zones. The right inset illustrates how the radial FWHM and the angular FWHM of the main lobe are determined from the PSF’s intensity distribution.

Fig. 3
Fig. 3

Flowchart of the extended depth-of-field image recovery and the depth estimation process. The captured raw image is divided into a series of sub-windows which are processed in parallel. The restoration process of each sub-window includes depth estimation and image reconstruction. The depth information is estimated by determining the maximal polar angle corresponding to the most densely populated cepstrum in a specific ring-shaped window. Meanwhile, the PSF corresponding to this sub-window can be estimated with the depth. The image is recovered by deconvolution with the estimated PSF. Depth map and image of the whole field-of-view can be recovered with ordered recombination of the results from all sub-windows.

Fig. 4
Fig. 4

Simulative results of image formation and restoration. (a) Simulated object composed of six lines of texts located at different depths. (b) and (c) Images formed with the conventional Gaussian PSF and the double-helix PSF (N = 6), respectively. (d) and (e) The recovered extended depth-of-field image and depth map from (c). The numeric aperture of the objective is set as 1.25 to keep in consistence with the experiment.

Fig. 5
Fig. 5

The impact of different aberrations on the double-helix PSF (simulated with N = 6, NA = 1.25, λ = 515nm). (a) The aberration-free PSF. (b)-(d) The distorted PSFs respectively calculated with primary spherical aberration, primary coma, and primary astigmatism. The top-right colorbar maps the intensity in the calculated images. To provide a quantitative impression of the distorted PSFs, the related data are attached on the bottom-right corner of each panel in (b), (c), and (d) respectively, which represent the rotation angle, the maximal intensity ratio of the main lobes, and the inter-lobe distance.

Fig. 6
Fig. 6

Experimental results of aberration correction. (a) Calculated intensity distribution of the adopted doughnut PSF with l = 2. (b) and (c) Measured intensity distribution of the doughnut PSF before and after aberration correction. The bottom-left inset of (c) shows the calculated hologram opposite to the aberration. (d) The vertical (blue) and horizontal (red) profile of the corrected doughnut PSF. (e) and (f) Measured intensity distribution of the double-helix PSF (N = 8) without and with aberration correction. The characteristic parameters including the rotation angle, the maximal intensity ratio of the main lobes, and the inter-lobe distance are respectively indicated at the top-right, top-left, and bottom-left corners of each panel.

Fig. 7
Fig. 7

Experimental observation results of a tilted slide of fluorescent beads with a diameter of 100nm. (a) and (b) are respectively the raw images acquired with the conventional Gaussian PSF and the double-helix PSF (N = 10). (c) and (d) are the reconstructed images of (b) with the Wiener-type filter and the Richardson-Lucy algorithm, respectively. For comparison, the magnified views of the dotted boxed regions marked with ROI1 and ROI2 in (a)-(d) are respectively shown in (a1)-(d1), and (a2)-(d2).

Fig. 8
Fig. 8

The double-helix PSF produces the extended depth-of-field recovered image, which is verified by the observation results of F-actin in BPAE cells. (a) and (b) are images captured by using the conventional Gaussian PSF as comparison. The two images are captured at different depths (1500nm apart). (c) The raw image acquired with the double-helix PSF (N = 6). (d) The recovered object image shown in (c). The magnified views of the dashed regions of interest marked with ROI1 (blue) and ROI2 (yellow) in (a)-(d) are respectively shown in (a1)–(d1), and (a2)–(d2), i.e., (a1), (b1), (c1) and (d1) are respectively zoomed in ROI1 from (a), (b), (c) and (d), while (a2), (b2), (c2) and (d2) are respectively zoomed in ROI2 from (a), (b), (c) and (d).

Fig. 9
Fig. 9

Estimated depth map corresponding to the FOV of Fig. 8(a) and 8(b) are estimated depth maps of an identical FOV, where map (b) is obtained after moving the specimen 300nm downward axially relative to that in (a). The patches marked with darkest blue in the depth map represent regions with no in-focus specimen. (c) presents the statistical histogram of the difference of the above two depth maps. Gaussian fit of the statistical data is calculated, implying a population standard deviation of 33nm. The precision of single measurement is thus calculated to be 23.4nm.

Equations (4)

Equations on this page are rendered with MathJax. Learn more.

P N (ρ,φ)={ exp[i(2n1)φ], R n1 N <ρR n N ,n=1,2,...,N 0, ρ>R ,
i(ξ,η)= o(x,y;z)*h(x,y;ξ,η;z)dz +n(ξ,η) ,
h(x,y;z)=rot[ h 0 (x,y+Δy/2)+ h 0 (x,yΔy/2), k θ(z) z] ,
h 0 (x,y)= 1 2π σ a σ r exp( x 2 2 σ a 2 y 2 2 σ r 2 ) ,