Abstract

Temporal focusing multiphoton microscopy (TFMPM) has the advantage of area excitation in an axial confinement of only a few microns; hence, it can offer fast three-dimensional (3D) multiphoton imaging. Herein, fast volumetric imaging via a developed digital micromirror device (DMD)-based TFMPM has been realized through the synchronization of an electron multiplying charge-coupled device (EMCCD) with a dynamic piezoelectric stage for axial scanning. The volumetric imaging rate can achieve 30 volumes per second according to the EMCCD frame rate of more than 400 frames per second, which allows for the 3D Brownian motion of one-micron fluorescent beads to be spatially observed. Furthermore, it is demonstrated that the dynamic HiLo structural multiphoton microscope can reject background noise by way of the fast volumetric imaging with high-speed DMD patterned illumination.

© 2016 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning

Li-Chung Cheng, Chia-Yuan Chang, Chun-Yu Lin, Keng-Chi Cho, Wei-Chung Yen, Nan-Shan Chang, Chris Xu, Chen Yuan Dong, and Shean-Jen Chen
Opt. Express 20(8) 8939-8948 (2012)

Nonlinear structured-illumination enhanced temporal focusing multiphoton excitation microscopy with a digital micromirror device

Li-Chung Cheng, Chi-Hsiang Lien, Yong Da Sie, Yvonne Yuling Hu, Chun-Yu Lin, Fan-Ching Chien, Chris Xu, Chen Yuan Dong, and Shean-Jen Chen
Biomed. Opt. Express 5(8) 2526-2536 (2014)

Temporal focusing-based multiphoton excitation microscopy via digital micromirror device

Jenq-Nan Yih, Yvonne Yuling Hu, Yong Da Sie, Li-Chung Cheng, Chi-Hsiang Lien, and Shean-Jen Chen
Opt. Lett. 39(11) 3134-3137 (2014)

References

  • View by:
  • |
  • |
  • |

  1. W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
    [Crossref] [PubMed]
  2. B. R. Masters and P. T. So, eds., Handbook of Biomedical Nonlinear Optical Microscopy (Oxford University Press, 2008).
  3. E. E. Hoover and J. A. Squier, “Advances in multiphoton microscopy technology,” Nat. Photonics 7(2), 93–101 (2013).
    [Crossref] [PubMed]
  4. I. Freund and M. Deutsch, “Second-harmonic microscopy of biological tissue,” Opt. Lett. 11(2), 94–96 (1986).
    [Crossref] [PubMed]
  5. F. S. Pavone and P. J. Campagnola, eds., Second Harmonic Generation Imaging (CRC Press, 2013).
  6. F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005).
    [Crossref] [PubMed]
  7. I. Veilleux, J. A. Spencer, D. P. Biss, D. Côté, and C. P. Lin, “In vivo cell tracking with video rate multimodality laser scanning microscopy,” IEEE J. Sel. Top. Quantum Electron. 14(1), 10–18 (2008).
    [Crossref]
  8. G. Duemani Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008).
    [Crossref] [PubMed]
  9. J. Bewersdorf, R. Pick, and S. W. Hell, “Multifocal multiphoton microscopy,” Opt. Lett. 23(9), 655–657 (1998).
    [Crossref] [PubMed]
  10. A. Cheng, J. T. Gonçalves, P. Golshani, K. Arisaka, and C. Portera-Cailliau, “Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing,” Nat. Methods 8(2), 139–142 (2011).
    [Crossref] [PubMed]
  11. B. F. Grewe, F. F. Voigt, M. van ’t Hoff, and F. Helmchen, “Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens,” Biomed. Opt. Express 2(7), 2035–2046 (2011).
    [Crossref] [PubMed]
  12. M. Duocastella, G. Vicidomini, and A. Diaspro, “Simultaneous multiplane confocal microscopy using acoustic tunable lenses,” Opt. Express 22(16), 19293–19301 (2014).
    [Crossref] [PubMed]
  13. L. Kong, J. Tang, J. P. Little, Y. Yu, T. Lämmermann, C. P. Lin, R. N. Germain, and M. Cui, “Continuous volumetric imaging via an optical phase-locked ultrasound lens,” Nat. Methods 12(8), 759–762 (2015).
    [Crossref] [PubMed]
  14. A. Kaplan, N. Friedman, and N. Davidson, “Acousto-optic lens with very fast focus scanning,” Opt. Lett. 26(14), 1078–1080 (2001).
    [Crossref] [PubMed]
  15. P. T. C. So, E. Y. S. Yew, and C. Rowlands, “High-throughput nonlinear optical microscopy,” Biophys. J. 105(12), 2641–2654 (2013).
    [Crossref] [PubMed]
  16. G. Zhu, J. van Howe, M. Durst, W. Zipfel, and C. Xu, “Simultaneous spatial and temporal focusing of femtosecond pulses,” Opt. Express 13(6), 2153–2159 (2005).
    [Crossref] [PubMed]
  17. D. Oron, E. Tal, and Y. Silberberg, “Scanningless depth-resolved microscopy,” Opt. Express 13(5), 1468–1476 (2005).
    [Crossref] [PubMed]
  18. O. D. Therrien, B. Aubé, S. Pagès, P. D. Koninck, and D. Côté, “Wide-field multiphoton imaging of cellular dynamics in thick tissue by temporal focusing and patterned illumination,” Biomed. Opt. Express 2(3), 696–704 (2011).
    [Crossref] [PubMed]
  19. L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express 20(8), 8939–8948 (2012).
    [Crossref] [PubMed]
  20. C. G. Durfee and J. A. Squier, “Breakthroughs in photonics 2014: Spatiotemporal focusing: Advances and applications,” IEEE Photonics J. 7(3), 0700806 (2015).
    [Crossref]
  21. H. Choi, D. S. Tzeranis, J. W. Cha, P. Clémenceau, S. J. de Jong, L. K. van Geest, J. H. Moon, I. V. Yannas, and P. T. So, “3D-resolved fluorescence and phosphorescence lifetime imaging using temporal focusing wide-field two-photon excitation,” Opt. Express 20(24), 26219–26235 (2012).
    [Crossref] [PubMed]
  22. C.-Y. Lin, P.-K. Li, L.-C. Cheng, Y.-C. Li, C.-Y. Chang, A.-S. Chiang, C. Y. Dong, and S.-J. Chen, “High-throughput multiphoton-induced three-dimensional ablation and imaging for biotissues,” Biomed. Opt. Express 6(2), 491–499 (2015).
    [Crossref] [PubMed]
  23. D. Kim and P. T. C. So, “High-throughput three-dimensional lithographic microfabrication,” Opt. Lett. 35(10), 1602–1604 (2010).
    [Crossref] [PubMed]
  24. E. Papagiakoumou, V. de Sars, D. Oron, and V. Emiliani, “Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses,” Opt. Express 16(26), 22039–22047 (2008).
    [Crossref] [PubMed]
  25. E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
    [Crossref] [PubMed]
  26. Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, “High-throughput fabrication of gray-level bio-microstructures via temporal focusing excitation and laser pulse control,” J. Biomed. Opt. 18(7), 075004 (2013).
    [Crossref]
  27. J.-N. Yih, Y. Y. Hu, Y. D. Sie, L.-C. Cheng, C.-H. Lien, and S.-J. Chen, “Temporal focusing-based multiphoton excitation microscopy via digital micromirror device,” Opt. Lett. 39(11), 3134–3137 (2014).
    [Crossref] [PubMed]
  28. L.-C. Cheng, C.-H. Lien, Y. Da Sie, Y. Y. Hu, C. Y. Lin, F. C. Chien, C. Xu, C. Y. Dong, and S. J. Chen, “Nonlinear structured-illumination enhanced temporal focusing multiphoton excitation microscopy with a digital micromirror device,” Biomed. Opt. Express 5(8), 2526–2536 (2014).
    [Crossref] [PubMed]
  29. A. Straub, M. E. Durst, and C. Xu, “High speed multiphoton axial scanning through an optical fiber in a remotely scanned temporal focusing setup,” Biomed. Opt. Express 2(1), 80–88 (2011).
    [Crossref] [PubMed]
  30. H. Dana and S. Shoham, “Remotely scanned multiphoton temporal focusing by axial grism scanning,” Opt. Lett. 37(14), 2913–2915 (2012).
    [Crossref] [PubMed]
  31. F. O. Fahrbach, F. F. Voigt, B. Schmid, F. Helmchen, and J. Huisken, “Rapid 3D light-sheet microscopy with a tunable lens,” Opt. Express 21(18), 21010–21026 (2013).
    [Crossref] [PubMed]
  32. J. Jiang, D. Zhang, S. Walker, C. Gu, Y. Ke, W. H. Yung, and S.-C. Chen, “Fast 3-D temporal focusing microscopy using an electrically tunable lens,” Opt. Express 23(19), 24362–24368 (2015).
    [Crossref] [PubMed]
  33. T. Schrödel, R. Prevedel, K. Aumayr, M. Zimmer, and A. Vaziri, “Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light,” Nat. Methods 10(10), 1013–1020 (2013).
    [Crossref] [PubMed]
  34. J. Mertz and J. Kim, “Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection,” J. Biomed. Opt. 15(1), 016027 (2010).
    [Crossref] [PubMed]
  35. V. Levi, Q. Ruan, and E. Gratton, “3-D particle tracking in a two-photon microscope: Application to the study of molecular dynamics in cells,” Biophys. J. 88(4), 2919–2928 (2005).
    [Crossref] [PubMed]
  36. R. M. Mazo, e.d., Brownian Motion: Fluctuations, Dynamics, and Applications (Oxford University Press, 2009).
  37. H. Choi, E. Y. S. Yew, B. Hallacoglu, S. Fantini, C. J. R. Sheppard, and P. T. C. So, “Improvement of axial resolution and contrast in temporally focused widefield two-photon microscopy with structured light illumination,” Biomed. Opt. Express 4(7), 995–1005 (2013).
    [Crossref] [PubMed]
  38. K. Isobe, T. Takeda, K. Mochizuki, Q. Song, A. Suda, F. Kannari, H. Kawano, A. Kumagai, A. Miyawaki, and K. Midorikawa, “Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination,” Biomed. Opt. Express 4(11), 2396–2410 (2013).
    [Crossref] [PubMed]

2015 (4)

L. Kong, J. Tang, J. P. Little, Y. Yu, T. Lämmermann, C. P. Lin, R. N. Germain, and M. Cui, “Continuous volumetric imaging via an optical phase-locked ultrasound lens,” Nat. Methods 12(8), 759–762 (2015).
[Crossref] [PubMed]

C. G. Durfee and J. A. Squier, “Breakthroughs in photonics 2014: Spatiotemporal focusing: Advances and applications,” IEEE Photonics J. 7(3), 0700806 (2015).
[Crossref]

C.-Y. Lin, P.-K. Li, L.-C. Cheng, Y.-C. Li, C.-Y. Chang, A.-S. Chiang, C. Y. Dong, and S.-J. Chen, “High-throughput multiphoton-induced three-dimensional ablation and imaging for biotissues,” Biomed. Opt. Express 6(2), 491–499 (2015).
[Crossref] [PubMed]

J. Jiang, D. Zhang, S. Walker, C. Gu, Y. Ke, W. H. Yung, and S.-C. Chen, “Fast 3-D temporal focusing microscopy using an electrically tunable lens,” Opt. Express 23(19), 24362–24368 (2015).
[Crossref] [PubMed]

2014 (3)

2013 (7)

H. Choi, E. Y. S. Yew, B. Hallacoglu, S. Fantini, C. J. R. Sheppard, and P. T. C. So, “Improvement of axial resolution and contrast in temporally focused widefield two-photon microscopy with structured light illumination,” Biomed. Opt. Express 4(7), 995–1005 (2013).
[Crossref] [PubMed]

F. O. Fahrbach, F. F. Voigt, B. Schmid, F. Helmchen, and J. Huisken, “Rapid 3D light-sheet microscopy with a tunable lens,” Opt. Express 21(18), 21010–21026 (2013).
[Crossref] [PubMed]

K. Isobe, T. Takeda, K. Mochizuki, Q. Song, A. Suda, F. Kannari, H. Kawano, A. Kumagai, A. Miyawaki, and K. Midorikawa, “Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination,” Biomed. Opt. Express 4(11), 2396–2410 (2013).
[Crossref] [PubMed]

P. T. C. So, E. Y. S. Yew, and C. Rowlands, “High-throughput nonlinear optical microscopy,” Biophys. J. 105(12), 2641–2654 (2013).
[Crossref] [PubMed]

E. E. Hoover and J. A. Squier, “Advances in multiphoton microscopy technology,” Nat. Photonics 7(2), 93–101 (2013).
[Crossref] [PubMed]

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, “High-throughput fabrication of gray-level bio-microstructures via temporal focusing excitation and laser pulse control,” J. Biomed. Opt. 18(7), 075004 (2013).
[Crossref]

T. Schrödel, R. Prevedel, K. Aumayr, M. Zimmer, and A. Vaziri, “Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light,” Nat. Methods 10(10), 1013–1020 (2013).
[Crossref] [PubMed]

2012 (3)

2011 (4)

2010 (3)

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

J. Mertz and J. Kim, “Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection,” J. Biomed. Opt. 15(1), 016027 (2010).
[Crossref] [PubMed]

D. Kim and P. T. C. So, “High-throughput three-dimensional lithographic microfabrication,” Opt. Lett. 35(10), 1602–1604 (2010).
[Crossref] [PubMed]

2008 (3)

E. Papagiakoumou, V. de Sars, D. Oron, and V. Emiliani, “Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses,” Opt. Express 16(26), 22039–22047 (2008).
[Crossref] [PubMed]

I. Veilleux, J. A. Spencer, D. P. Biss, D. Côté, and C. P. Lin, “In vivo cell tracking with video rate multimodality laser scanning microscopy,” IEEE J. Sel. Top. Quantum Electron. 14(1), 10–18 (2008).
[Crossref]

G. Duemani Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008).
[Crossref] [PubMed]

2005 (4)

V. Levi, Q. Ruan, and E. Gratton, “3-D particle tracking in a two-photon microscope: Application to the study of molecular dynamics in cells,” Biophys. J. 88(4), 2919–2928 (2005).
[Crossref] [PubMed]

F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005).
[Crossref] [PubMed]

D. Oron, E. Tal, and Y. Silberberg, “Scanningless depth-resolved microscopy,” Opt. Express 13(5), 1468–1476 (2005).
[Crossref] [PubMed]

G. Zhu, J. van Howe, M. Durst, W. Zipfel, and C. Xu, “Simultaneous spatial and temporal focusing of femtosecond pulses,” Opt. Express 13(6), 2153–2159 (2005).
[Crossref] [PubMed]

2003 (1)

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[Crossref] [PubMed]

2001 (1)

1998 (1)

1986 (1)

Anselmi, F.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

Arisaka, K.

A. Cheng, J. T. Gonçalves, P. Golshani, K. Arisaka, and C. Portera-Cailliau, “Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing,” Nat. Methods 8(2), 139–142 (2011).
[Crossref] [PubMed]

Aubé, B.

Aumayr, K.

T. Schrödel, R. Prevedel, K. Aumayr, M. Zimmer, and A. Vaziri, “Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light,” Nat. Methods 10(10), 1013–1020 (2013).
[Crossref] [PubMed]

Bègue, A.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

Bewersdorf, J.

Biss, D. P.

I. Veilleux, J. A. Spencer, D. P. Biss, D. Côté, and C. P. Lin, “In vivo cell tracking with video rate multimodality laser scanning microscopy,” IEEE J. Sel. Top. Quantum Electron. 14(1), 10–18 (2008).
[Crossref]

Campagnola, P. J.

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, “High-throughput fabrication of gray-level bio-microstructures via temporal focusing excitation and laser pulse control,” J. Biomed. Opt. 18(7), 075004 (2013).
[Crossref]

Cha, J. W.

Chang, C.-Y.

Chang, N.-S.

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, “High-throughput fabrication of gray-level bio-microstructures via temporal focusing excitation and laser pulse control,” J. Biomed. Opt. 18(7), 075004 (2013).
[Crossref]

L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express 20(8), 8939–8948 (2012).
[Crossref] [PubMed]

Chen, S. J.

Chen, S.-C.

Chen, S.-J.

Cheng, A.

A. Cheng, J. T. Gonçalves, P. Golshani, K. Arisaka, and C. Portera-Cailliau, “Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing,” Nat. Methods 8(2), 139–142 (2011).
[Crossref] [PubMed]

Cheng, L.-C.

Chiang, A.-S.

Chien, F. C.

Cho, K.-C.

Choi, H.

Clémenceau, P.

Côté, D.

O. D. Therrien, B. Aubé, S. Pagès, P. D. Koninck, and D. Côté, “Wide-field multiphoton imaging of cellular dynamics in thick tissue by temporal focusing and patterned illumination,” Biomed. Opt. Express 2(3), 696–704 (2011).
[Crossref] [PubMed]

I. Veilleux, J. A. Spencer, D. P. Biss, D. Côté, and C. P. Lin, “In vivo cell tracking with video rate multimodality laser scanning microscopy,” IEEE J. Sel. Top. Quantum Electron. 14(1), 10–18 (2008).
[Crossref]

Cui, M.

L. Kong, J. Tang, J. P. Little, Y. Yu, T. Lämmermann, C. P. Lin, R. N. Germain, and M. Cui, “Continuous volumetric imaging via an optical phase-locked ultrasound lens,” Nat. Methods 12(8), 759–762 (2015).
[Crossref] [PubMed]

Da Sie, Y.

Dana, H.

Davidson, N.

de Jong, S. J.

de Sars, V.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

E. Papagiakoumou, V. de Sars, D. Oron, and V. Emiliani, “Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses,” Opt. Express 16(26), 22039–22047 (2008).
[Crossref] [PubMed]

Denk, W.

F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005).
[Crossref] [PubMed]

Deutsch, M.

Diaspro, A.

Dong, C. Y.

Duemani Reddy, G.

G. Duemani Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008).
[Crossref] [PubMed]

Duocastella, M.

Durfee, C. G.

C. G. Durfee and J. A. Squier, “Breakthroughs in photonics 2014: Spatiotemporal focusing: Advances and applications,” IEEE Photonics J. 7(3), 0700806 (2015).
[Crossref]

Durst, M.

Durst, M. E.

Emiliani, V.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

E. Papagiakoumou, V. de Sars, D. Oron, and V. Emiliani, “Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses,” Opt. Express 16(26), 22039–22047 (2008).
[Crossref] [PubMed]

Fahrbach, F. O.

Fantini, S.

Fink, R.

G. Duemani Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008).
[Crossref] [PubMed]

Freund, I.

Friedman, N.

Germain, R. N.

L. Kong, J. Tang, J. P. Little, Y. Yu, T. Lämmermann, C. P. Lin, R. N. Germain, and M. Cui, “Continuous volumetric imaging via an optical phase-locked ultrasound lens,” Nat. Methods 12(8), 759–762 (2015).
[Crossref] [PubMed]

Glückstad, J.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

Golshani, P.

A. Cheng, J. T. Gonçalves, P. Golshani, K. Arisaka, and C. Portera-Cailliau, “Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing,” Nat. Methods 8(2), 139–142 (2011).
[Crossref] [PubMed]

Gonçalves, J. T.

A. Cheng, J. T. Gonçalves, P. Golshani, K. Arisaka, and C. Portera-Cailliau, “Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing,” Nat. Methods 8(2), 139–142 (2011).
[Crossref] [PubMed]

Gratton, E.

V. Levi, Q. Ruan, and E. Gratton, “3-D particle tracking in a two-photon microscope: Application to the study of molecular dynamics in cells,” Biophys. J. 88(4), 2919–2928 (2005).
[Crossref] [PubMed]

Grewe, B. F.

Gu, C.

Hallacoglu, B.

Hell, S. W.

Helmchen, F.

Hoover, E. E.

E. E. Hoover and J. A. Squier, “Advances in multiphoton microscopy technology,” Nat. Photonics 7(2), 93–101 (2013).
[Crossref] [PubMed]

Hu, Y. Y.

Huisken, J.

Isacoff, E. Y.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

Isobe, K.

Jiang, J.

Kannari, F.

Kaplan, A.

Kawano, H.

Ke, Y.

Kelleher, K.

G. Duemani Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008).
[Crossref] [PubMed]

Kim, D.

Kim, J.

J. Mertz and J. Kim, “Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection,” J. Biomed. Opt. 15(1), 016027 (2010).
[Crossref] [PubMed]

Kong, L.

L. Kong, J. Tang, J. P. Little, Y. Yu, T. Lämmermann, C. P. Lin, R. N. Germain, and M. Cui, “Continuous volumetric imaging via an optical phase-locked ultrasound lens,” Nat. Methods 12(8), 759–762 (2015).
[Crossref] [PubMed]

Koninck, P. D.

Kumagai, A.

Lämmermann, T.

L. Kong, J. Tang, J. P. Little, Y. Yu, T. Lämmermann, C. P. Lin, R. N. Germain, and M. Cui, “Continuous volumetric imaging via an optical phase-locked ultrasound lens,” Nat. Methods 12(8), 759–762 (2015).
[Crossref] [PubMed]

Levi, V.

V. Levi, Q. Ruan, and E. Gratton, “3-D particle tracking in a two-photon microscope: Application to the study of molecular dynamics in cells,” Biophys. J. 88(4), 2919–2928 (2005).
[Crossref] [PubMed]

Li, P.-K.

Li, Y.-C.

C.-Y. Lin, P.-K. Li, L.-C. Cheng, Y.-C. Li, C.-Y. Chang, A.-S. Chiang, C. Y. Dong, and S.-J. Chen, “High-throughput multiphoton-induced three-dimensional ablation and imaging for biotissues,” Biomed. Opt. Express 6(2), 491–499 (2015).
[Crossref] [PubMed]

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, “High-throughput fabrication of gray-level bio-microstructures via temporal focusing excitation and laser pulse control,” J. Biomed. Opt. 18(7), 075004 (2013).
[Crossref]

Lien, C.-H.

Lin, C. P.

L. Kong, J. Tang, J. P. Little, Y. Yu, T. Lämmermann, C. P. Lin, R. N. Germain, and M. Cui, “Continuous volumetric imaging via an optical phase-locked ultrasound lens,” Nat. Methods 12(8), 759–762 (2015).
[Crossref] [PubMed]

I. Veilleux, J. A. Spencer, D. P. Biss, D. Côté, and C. P. Lin, “In vivo cell tracking with video rate multimodality laser scanning microscopy,” IEEE J. Sel. Top. Quantum Electron. 14(1), 10–18 (2008).
[Crossref]

Lin, C. Y.

Lin, C.-Y.

Little, J. P.

L. Kong, J. Tang, J. P. Little, Y. Yu, T. Lämmermann, C. P. Lin, R. N. Germain, and M. Cui, “Continuous volumetric imaging via an optical phase-locked ultrasound lens,” Nat. Methods 12(8), 759–762 (2015).
[Crossref] [PubMed]

Mertz, J.

J. Mertz and J. Kim, “Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection,” J. Biomed. Opt. 15(1), 016027 (2010).
[Crossref] [PubMed]

Midorikawa, K.

Miyawaki, A.

Mochizuki, K.

Moon, J. H.

Oron, D.

Pagès, S.

Papagiakoumou, E.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

E. Papagiakoumou, V. de Sars, D. Oron, and V. Emiliani, “Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses,” Opt. Express 16(26), 22039–22047 (2008).
[Crossref] [PubMed]

Pick, R.

Portera-Cailliau, C.

A. Cheng, J. T. Gonçalves, P. Golshani, K. Arisaka, and C. Portera-Cailliau, “Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing,” Nat. Methods 8(2), 139–142 (2011).
[Crossref] [PubMed]

Prevedel, R.

T. Schrödel, R. Prevedel, K. Aumayr, M. Zimmer, and A. Vaziri, “Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light,” Nat. Methods 10(10), 1013–1020 (2013).
[Crossref] [PubMed]

Rowlands, C.

P. T. C. So, E. Y. S. Yew, and C. Rowlands, “High-throughput nonlinear optical microscopy,” Biophys. J. 105(12), 2641–2654 (2013).
[Crossref] [PubMed]

Ruan, Q.

V. Levi, Q. Ruan, and E. Gratton, “3-D particle tracking in a two-photon microscope: Application to the study of molecular dynamics in cells,” Biophys. J. 88(4), 2919–2928 (2005).
[Crossref] [PubMed]

Saggau, P.

G. Duemani Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008).
[Crossref] [PubMed]

Schmid, B.

Schrödel, T.

T. Schrödel, R. Prevedel, K. Aumayr, M. Zimmer, and A. Vaziri, “Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light,” Nat. Methods 10(10), 1013–1020 (2013).
[Crossref] [PubMed]

Sheppard, C. J. R.

Shoham, S.

Sie, Y. D.

Silberberg, Y.

So, P. T.

So, P. T. C.

Song, Q.

Spencer, J. A.

I. Veilleux, J. A. Spencer, D. P. Biss, D. Côté, and C. P. Lin, “In vivo cell tracking with video rate multimodality laser scanning microscopy,” IEEE J. Sel. Top. Quantum Electron. 14(1), 10–18 (2008).
[Crossref]

Squier, J. A.

C. G. Durfee and J. A. Squier, “Breakthroughs in photonics 2014: Spatiotemporal focusing: Advances and applications,” IEEE Photonics J. 7(3), 0700806 (2015).
[Crossref]

E. E. Hoover and J. A. Squier, “Advances in multiphoton microscopy technology,” Nat. Photonics 7(2), 93–101 (2013).
[Crossref] [PubMed]

Straub, A.

Suda, A.

Takeda, T.

Tal, E.

Tang, J.

L. Kong, J. Tang, J. P. Little, Y. Yu, T. Lämmermann, C. P. Lin, R. N. Germain, and M. Cui, “Continuous volumetric imaging via an optical phase-locked ultrasound lens,” Nat. Methods 12(8), 759–762 (2015).
[Crossref] [PubMed]

Therrien, O. D.

Tzeranis, D. S.

van ’t Hoff, M.

van Geest, L. K.

van Howe, J.

Vaziri, A.

T. Schrödel, R. Prevedel, K. Aumayr, M. Zimmer, and A. Vaziri, “Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light,” Nat. Methods 10(10), 1013–1020 (2013).
[Crossref] [PubMed]

Veilleux, I.

I. Veilleux, J. A. Spencer, D. P. Biss, D. Côté, and C. P. Lin, “In vivo cell tracking with video rate multimodality laser scanning microscopy,” IEEE J. Sel. Top. Quantum Electron. 14(1), 10–18 (2008).
[Crossref]

Vicidomini, G.

Voigt, F. F.

Walker, S.

Webb, W. W.

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[Crossref] [PubMed]

Williams, R. M.

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[Crossref] [PubMed]

Xu, C.

Yannas, I. V.

Yen, W.-C.

Yew, E. Y. S.

Yih, J.-N.

Yu, Y.

L. Kong, J. Tang, J. P. Little, Y. Yu, T. Lämmermann, C. P. Lin, R. N. Germain, and M. Cui, “Continuous volumetric imaging via an optical phase-locked ultrasound lens,” Nat. Methods 12(8), 759–762 (2015).
[Crossref] [PubMed]

Yung, W. H.

Zhang, D.

Zhu, G.

Zimmer, M.

T. Schrödel, R. Prevedel, K. Aumayr, M. Zimmer, and A. Vaziri, “Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light,” Nat. Methods 10(10), 1013–1020 (2013).
[Crossref] [PubMed]

Zipfel, W.

Zipfel, W. R.

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[Crossref] [PubMed]

Biomed. Opt. Express (7)

A. Straub, M. E. Durst, and C. Xu, “High speed multiphoton axial scanning through an optical fiber in a remotely scanned temporal focusing setup,” Biomed. Opt. Express 2(1), 80–88 (2011).
[Crossref] [PubMed]

O. D. Therrien, B. Aubé, S. Pagès, P. D. Koninck, and D. Côté, “Wide-field multiphoton imaging of cellular dynamics in thick tissue by temporal focusing and patterned illumination,” Biomed. Opt. Express 2(3), 696–704 (2011).
[Crossref] [PubMed]

B. F. Grewe, F. F. Voigt, M. van ’t Hoff, and F. Helmchen, “Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens,” Biomed. Opt. Express 2(7), 2035–2046 (2011).
[Crossref] [PubMed]

H. Choi, E. Y. S. Yew, B. Hallacoglu, S. Fantini, C. J. R. Sheppard, and P. T. C. So, “Improvement of axial resolution and contrast in temporally focused widefield two-photon microscopy with structured light illumination,” Biomed. Opt. Express 4(7), 995–1005 (2013).
[Crossref] [PubMed]

K. Isobe, T. Takeda, K. Mochizuki, Q. Song, A. Suda, F. Kannari, H. Kawano, A. Kumagai, A. Miyawaki, and K. Midorikawa, “Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination,” Biomed. Opt. Express 4(11), 2396–2410 (2013).
[Crossref] [PubMed]

L.-C. Cheng, C.-H. Lien, Y. Da Sie, Y. Y. Hu, C. Y. Lin, F. C. Chien, C. Xu, C. Y. Dong, and S. J. Chen, “Nonlinear structured-illumination enhanced temporal focusing multiphoton excitation microscopy with a digital micromirror device,” Biomed. Opt. Express 5(8), 2526–2536 (2014).
[Crossref] [PubMed]

C.-Y. Lin, P.-K. Li, L.-C. Cheng, Y.-C. Li, C.-Y. Chang, A.-S. Chiang, C. Y. Dong, and S.-J. Chen, “High-throughput multiphoton-induced three-dimensional ablation and imaging for biotissues,” Biomed. Opt. Express 6(2), 491–499 (2015).
[Crossref] [PubMed]

Biophys. J. (2)

P. T. C. So, E. Y. S. Yew, and C. Rowlands, “High-throughput nonlinear optical microscopy,” Biophys. J. 105(12), 2641–2654 (2013).
[Crossref] [PubMed]

V. Levi, Q. Ruan, and E. Gratton, “3-D particle tracking in a two-photon microscope: Application to the study of molecular dynamics in cells,” Biophys. J. 88(4), 2919–2928 (2005).
[Crossref] [PubMed]

IEEE J. Sel. Top. Quantum Electron. (1)

I. Veilleux, J. A. Spencer, D. P. Biss, D. Côté, and C. P. Lin, “In vivo cell tracking with video rate multimodality laser scanning microscopy,” IEEE J. Sel. Top. Quantum Electron. 14(1), 10–18 (2008).
[Crossref]

IEEE Photonics J. (1)

C. G. Durfee and J. A. Squier, “Breakthroughs in photonics 2014: Spatiotemporal focusing: Advances and applications,” IEEE Photonics J. 7(3), 0700806 (2015).
[Crossref]

J. Biomed. Opt. (2)

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, “High-throughput fabrication of gray-level bio-microstructures via temporal focusing excitation and laser pulse control,” J. Biomed. Opt. 18(7), 075004 (2013).
[Crossref]

J. Mertz and J. Kim, “Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection,” J. Biomed. Opt. 15(1), 016027 (2010).
[Crossref] [PubMed]

Nat. Biotechnol. (1)

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[Crossref] [PubMed]

Nat. Methods (5)

A. Cheng, J. T. Gonçalves, P. Golshani, K. Arisaka, and C. Portera-Cailliau, “Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing,” Nat. Methods 8(2), 139–142 (2011).
[Crossref] [PubMed]

L. Kong, J. Tang, J. P. Little, Y. Yu, T. Lämmermann, C. P. Lin, R. N. Germain, and M. Cui, “Continuous volumetric imaging via an optical phase-locked ultrasound lens,” Nat. Methods 12(8), 759–762 (2015).
[Crossref] [PubMed]

F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005).
[Crossref] [PubMed]

T. Schrödel, R. Prevedel, K. Aumayr, M. Zimmer, and A. Vaziri, “Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light,” Nat. Methods 10(10), 1013–1020 (2013).
[Crossref] [PubMed]

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

Nat. Neurosci. (1)

G. Duemani Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008).
[Crossref] [PubMed]

Nat. Photonics (1)

E. E. Hoover and J. A. Squier, “Advances in multiphoton microscopy technology,” Nat. Photonics 7(2), 93–101 (2013).
[Crossref] [PubMed]

Opt. Express (8)

M. Duocastella, G. Vicidomini, and A. Diaspro, “Simultaneous multiplane confocal microscopy using acoustic tunable lenses,” Opt. Express 22(16), 19293–19301 (2014).
[Crossref] [PubMed]

F. O. Fahrbach, F. F. Voigt, B. Schmid, F. Helmchen, and J. Huisken, “Rapid 3D light-sheet microscopy with a tunable lens,” Opt. Express 21(18), 21010–21026 (2013).
[Crossref] [PubMed]

L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express 20(8), 8939–8948 (2012).
[Crossref] [PubMed]

D. Oron, E. Tal, and Y. Silberberg, “Scanningless depth-resolved microscopy,” Opt. Express 13(5), 1468–1476 (2005).
[Crossref] [PubMed]

G. Zhu, J. van Howe, M. Durst, W. Zipfel, and C. Xu, “Simultaneous spatial and temporal focusing of femtosecond pulses,” Opt. Express 13(6), 2153–2159 (2005).
[Crossref] [PubMed]

E. Papagiakoumou, V. de Sars, D. Oron, and V. Emiliani, “Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses,” Opt. Express 16(26), 22039–22047 (2008).
[Crossref] [PubMed]

J. Jiang, D. Zhang, S. Walker, C. Gu, Y. Ke, W. H. Yung, and S.-C. Chen, “Fast 3-D temporal focusing microscopy using an electrically tunable lens,” Opt. Express 23(19), 24362–24368 (2015).
[Crossref] [PubMed]

H. Choi, D. S. Tzeranis, J. W. Cha, P. Clémenceau, S. J. de Jong, L. K. van Geest, J. H. Moon, I. V. Yannas, and P. T. So, “3D-resolved fluorescence and phosphorescence lifetime imaging using temporal focusing wide-field two-photon excitation,” Opt. Express 20(24), 26219–26235 (2012).
[Crossref] [PubMed]

Opt. Lett. (6)

Other (3)

F. S. Pavone and P. J. Campagnola, eds., Second Harmonic Generation Imaging (CRC Press, 2013).

B. R. Masters and P. T. So, eds., Handbook of Biomedical Nonlinear Optical Microscopy (Oxford University Press, 2008).

R. M. Mazo, e.d., Brownian Motion: Fluctuations, Dynamics, and Applications (Oxford University Press, 2009).

Supplementary Material (3)

NameDescription
» Visualization 1: AVI (14652 KB)      Video 1
» Visualization 2: AVI (2452 KB)      Video 2
» Visualization 3: AVI (4397 KB)      Video 3

Cited By

OSA participates in Crossref's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (6)

Fig. 1
Fig. 1

Optical setup of DMD-based TFMPM.

Fig. 2
Fig. 2

Axial TPEF fluorescence intensity profile. Circles represent the average intensity of the images at different depths, while the solid line is the fitted curve. The estimated axial size of the excitation volume is 2.8 μm at FWHM.

Fig. 3
Fig. 3

Volumetric imaging of a 10 μm fluorescent bead. (a) Averaged axial fluorescence intensity profile with estimated fluorescent bead size of 10.4 μm at FWHM. (b) The EMCCD camera acquired frame positions. Blue and red circles represent the rising and falling portions of the sinusoidal waveform via the piezo-stage driving, respectively.

Fig. 4
Fig. 4

Brownian motion trajectory of 1 μm fluorescent bead. (a) Front view (x-y plane); and (b) side view (y-z plane). The 3D rendered movie is shown in Visualization 2. (c) Trajectory of a single fluorescent bead. Blue, green, and red curves are for x-, y-, and z-position, respectively. (d) MSD versus time. Circles are the measured data at different times with a fitting line.

Fig. 5
Fig. 5

Optical pattering verification. (a) Fluorescent images via four iterative patterns applied to the DMD to excite the auto-fluorescent plastic slide. (b) The fluorescence intensity modulation at different volume numbers. Top: the average intensity inside the blue-dashed square of Fig. 5(a); and bottom: inside the red-dashed square of Fig. 5(a).

Fig. 6
Fig. 6

Fluorescence images of H&E stained back skin from the pathological section of a New Zealand white rabbit. (a) A sinusoidal pattern on fluorescent thin film, (b) the sinusoidal pattern on specimen, (c) the raw fluorescence image of a single section, and (d) the HiLo image of the same section. The rendered 3D images are shown in Visualization 3. (e) Normalized intensity profiles of the red-dashed lines in Figs. 6(c) and 6(d). Blue curve: raw image; red curve: HiLo image.

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

s[n]= A 2 ( sin( 2πf(n+p) f s )+1 )(u[n]u[nm]),
D= k B T 6πηr ,
MSD(t)=6Dt.

Metrics