Abstract

Circulating tumor cell clusters (CTCCs) are significantly more likely to form metastases than single tumor cells. We demonstrate the potential of backscatter-based flow cytometry (BSFC) to detect unique light scattering signatures of CTCCs in the blood of mice orthotopically implanted with breast cancer cells and treated with an anti-ADAM8 or a control antibody. Based on scattering detected at 405, 488, and 633 nm from blood samples flowing through microfluidic devices, we identified 14 CTCCs with large scattering peak widths and intensities, whose presence correlated strongly with metastasis. These initial studies demonstrate the potential to detect CTCCs via label-free BSFC.

© 2016 Optical Society of America

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References

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  1. P. Mehlen and A. Puisieux, “Metastasis: a question of life or death,” Nat. Rev. Cancer 6(6), 449–458 (2006).
    [Crossref] [PubMed]
  2. S. Valastyan and R. A. Weinberg, “Tumor metastasis: molecular insights and evolving paradigms,” Cell 147(2), 275–292 (2011).
    [Crossref] [PubMed]
  3. M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
    [Crossref] [PubMed]
  4. M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
    [Crossref] [PubMed]
  5. L. Sherwood, Human Physiology: From Cells to Systems, 9th ed. (Cengage Learning, Boston,MA, 2015), p. 393.
  6. S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
    [Crossref] [PubMed]
  7. I. Van der Auwera, D. Peeters, I. H. Benoy, H. J. Elst, S. J. Van Laere, A. Prové, H. Maes, P. Huget, P. van Dam, P. B. Vermeulen, and L. Y. Dirix, “Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer,” Br. J. Cancer 102(2), 276–284 (2010).
    [Crossref] [PubMed]
  8. S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
    [Crossref] [PubMed]
  9. C. G. Rao, D. Chianese, G. V. Doyle, M. C. Miller, T. Russell, R. A. J. Sanders, and L. W. M. M. Terstappen, “Expression of epithelial cell adhesion molecule in carcinoma cells present in blood and primary and metastatic tumors,” Int. J. Oncol. 27(1), 49–57 (2005).
    [PubMed]
  10. N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
    [Crossref] [PubMed]
  11. N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
    [Crossref] [PubMed]
  12. A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
    [Crossref] [PubMed]
  13. J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, “In vivo flow cytometer for real-time detection and quantification of circulating cells,” Opt. Lett. 29(1), 77–79 (2004).
    [Crossref] [PubMed]
  14. D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment,” Nature 435(7044), 969–973 (2005).
    [Crossref] [PubMed]
  15. S. Boutrus, C. Greiner, D. Hwu, M. Chan, C. Kuperwasser, C. P. Lin, and I. Georgakoudi, “Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells,” J. Biomed. Opt. 12(2), 020507 (2007).
    [Crossref] [PubMed]
  16. D. Hwu, S. Boutrus, C. Greiner, T. DiMeo, C. Kuperwasser, and I. Georgakoudi, “Assessment of the role of circulating breast cancer cells in tumor formation and metastatic potential using in vivo flow cytometry,” J. Biomed. Opt. 16(4), 040501 (2011).
    [Crossref] [PubMed]
  17. S. Markovic, B. Li, V. Pera, M. Sznaier, O. Camps, and M. Niedre, “A computer vision approach to rare cell in vivo fluorescence flow cytometry,” Cytometry A 83(12), 1113–1123 (2013).
    [Crossref] [PubMed]
  18. I. Georgakoudi, N. Solban, J. Novak, W. L. Rice, X. Wei, T. Hasan, and C. P. Lin, “In vivo flow cytometry: a new method for enumerating circulating cancer cells advances in brief in vivo flow cytometry : a new method for enumerating circulating cancer cells,” Cancer Res. 64(15), 5044–5047 (2004).
    [Crossref] [PubMed]
  19. T. T. Le, T. B. Huff, and J.-X. Cheng, “Coherent anti-Stokes Raman scattering imaging of lipids in cancer metastasis,” BMC Cancer 9(1), 42 (2009).
    [Crossref] [PubMed]
  20. E. I. Galanzha, E. V. Shashkov, P. M. Spring, J. Y. Suen, and V. P. Zharov, “In vivo, noninvasive, label-free detection and eradication of circulating metastatic melanoma cells using two-color photoacoustic flow cytometry with a diode laser,” Cancer Res. 69(20), 7926–7934 (2009).
    [Crossref] [PubMed]
  21. G. Koller, U. Schlomann, P. Golfi, T. Ferdous, S. Naus, and J. W. Bartsch, “ADAM8/MS2/CD156, an Emerging Drug Target in the Treatment of Inflammatory and Invasive Pathologies,” Curr. Pharm. Des. 15(20), 2272–2281 (2009).
    [Crossref] [PubMed]
  22. N. E. King, N. Zimmermann, S. M. Pope, P. C. Fulkerson, N. M. Nikolaidis, A. Mishra, D. P. Witte, and M. E. Rothenberg, “Expression and regulation of a disintegrin and metalloproteinase (ADAM) 8 in experimental asthma,” Am. J. Respir. Cell Mol. Biol. 31(3), 257–265 (2004).
    [Crossref] [PubMed]
  23. M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
    [PubMed]
  24. C. Greiner, M. Hunter, F. Rius, P. Huang, and I. Georgakoudi, “Confocal backscattering-based detection of leukemic cells in flowing blood samples,” Cytometry A 79(10), 874–883 (2011).
    [Crossref] [PubMed]
  25. R. F. Woolson, Statistical Methods for the Analysis of Biomedical Data, Wiley Series in Probability and Mathematical Statistics (Wiley, New York, NY, 1987).
  26. K. Fišer, T. Sieger, A. Schumich, B. Wood, J. Irving, E. Mejstříková, and M. N. Dworzak, “Detection and monitoring of normal and leukemic cell populations with hierarchical clustering of flow cytometry data,” Cytometry A 81(1), 25–34 (2012).
    [Crossref] [PubMed]
  27. S. M. Pantanelli, Z. Li, R. Fariss, S. P. Mahesh, B. Liu, and R. B. Nussenblatt, “Differentiation of malignant B-lymphoma cells from normal and activated T-cell populations by their intrinsic autofluorescence,” Cancer Res. 69(11), 4911–4917 (2009).
    [Crossref] [PubMed]
  28. J. M. Njoroge, L. B. Mitchell, M. Centola, D. Kastner, M. Raffeld, and J. L. Miller, “Characterization of viable autofluorescent macrophages among cultured peripheral blood mononuclear cells,” Cytometry 44(1), 38–44 (2001).
    [Crossref] [PubMed]
  29. M. Monici, R. Pratesi, P. A. Bernabei, R. Caporale, P. R. Ferrini, A. C. Croce, P. Balzarini, and G. Bottiroli, “Natural fluorescence of white blood cells: spectroscopic and imaging study,” J. Photochem. Photobiol. B 30(1), 29–37 (1995).
    [Crossref] [PubMed]
  30. R. C. Benson, R. A. Meyer, M. E. Zaruba, and G. M. McKhann, “Cellular autofluorescence--is it due to flavins?” J. Histochem. Cytochem. 27(1), 44–48 (1979).
    [Crossref] [PubMed]
  31. A. N. Mayeno, K. J. Hamann, and G. J. Gleich, “Granule-associated flavin adenine dinucleotide (FAD) is responsible for eosinophil autofluorescence,” J. Leukoc. Biol. 51(2), 172–175 (1992).
    [PubMed]

2015 (1)

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

2014 (3)

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

2013 (1)

S. Markovic, B. Li, V. Pera, M. Sznaier, O. Camps, and M. Niedre, “A computer vision approach to rare cell in vivo fluorescence flow cytometry,” Cytometry A 83(12), 1113–1123 (2013).
[Crossref] [PubMed]

2012 (1)

K. Fišer, T. Sieger, A. Schumich, B. Wood, J. Irving, E. Mejstříková, and M. N. Dworzak, “Detection and monitoring of normal and leukemic cell populations with hierarchical clustering of flow cytometry data,” Cytometry A 81(1), 25–34 (2012).
[Crossref] [PubMed]

2011 (4)

C. Greiner, M. Hunter, F. Rius, P. Huang, and I. Georgakoudi, “Confocal backscattering-based detection of leukemic cells in flowing blood samples,” Cytometry A 79(10), 874–883 (2011).
[Crossref] [PubMed]

D. Hwu, S. Boutrus, C. Greiner, T. DiMeo, C. Kuperwasser, and I. Georgakoudi, “Assessment of the role of circulating breast cancer cells in tumor formation and metastatic potential using in vivo flow cytometry,” J. Biomed. Opt. 16(4), 040501 (2011).
[Crossref] [PubMed]

S. Valastyan and R. A. Weinberg, “Tumor metastasis: molecular insights and evolving paradigms,” Cell 147(2), 275–292 (2011).
[Crossref] [PubMed]

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

2010 (2)

I. Van der Auwera, D. Peeters, I. H. Benoy, H. J. Elst, S. J. Van Laere, A. Prové, H. Maes, P. Huget, P. van Dam, P. B. Vermeulen, and L. Y. Dirix, “Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer,” Br. J. Cancer 102(2), 276–284 (2010).
[Crossref] [PubMed]

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

2009 (4)

S. M. Pantanelli, Z. Li, R. Fariss, S. P. Mahesh, B. Liu, and R. B. Nussenblatt, “Differentiation of malignant B-lymphoma cells from normal and activated T-cell populations by their intrinsic autofluorescence,” Cancer Res. 69(11), 4911–4917 (2009).
[Crossref] [PubMed]

T. T. Le, T. B. Huff, and J.-X. Cheng, “Coherent anti-Stokes Raman scattering imaging of lipids in cancer metastasis,” BMC Cancer 9(1), 42 (2009).
[Crossref] [PubMed]

E. I. Galanzha, E. V. Shashkov, P. M. Spring, J. Y. Suen, and V. P. Zharov, “In vivo, noninvasive, label-free detection and eradication of circulating metastatic melanoma cells using two-color photoacoustic flow cytometry with a diode laser,” Cancer Res. 69(20), 7926–7934 (2009).
[Crossref] [PubMed]

G. Koller, U. Schlomann, P. Golfi, T. Ferdous, S. Naus, and J. W. Bartsch, “ADAM8/MS2/CD156, an Emerging Drug Target in the Treatment of Inflammatory and Invasive Pathologies,” Curr. Pharm. Des. 15(20), 2272–2281 (2009).
[Crossref] [PubMed]

2007 (2)

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

S. Boutrus, C. Greiner, D. Hwu, M. Chan, C. Kuperwasser, C. P. Lin, and I. Georgakoudi, “Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells,” J. Biomed. Opt. 12(2), 020507 (2007).
[Crossref] [PubMed]

2006 (1)

P. Mehlen and A. Puisieux, “Metastasis: a question of life or death,” Nat. Rev. Cancer 6(6), 449–458 (2006).
[Crossref] [PubMed]

2005 (3)

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment,” Nature 435(7044), 969–973 (2005).
[Crossref] [PubMed]

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

C. G. Rao, D. Chianese, G. V. Doyle, M. C. Miller, T. Russell, R. A. J. Sanders, and L. W. M. M. Terstappen, “Expression of epithelial cell adhesion molecule in carcinoma cells present in blood and primary and metastatic tumors,” Int. J. Oncol. 27(1), 49–57 (2005).
[PubMed]

2004 (3)

J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, “In vivo flow cytometer for real-time detection and quantification of circulating cells,” Opt. Lett. 29(1), 77–79 (2004).
[Crossref] [PubMed]

I. Georgakoudi, N. Solban, J. Novak, W. L. Rice, X. Wei, T. Hasan, and C. P. Lin, “In vivo flow cytometry: a new method for enumerating circulating cancer cells advances in brief in vivo flow cytometry : a new method for enumerating circulating cancer cells,” Cancer Res. 64(15), 5044–5047 (2004).
[Crossref] [PubMed]

N. E. King, N. Zimmermann, S. M. Pope, P. C. Fulkerson, N. M. Nikolaidis, A. Mishra, D. P. Witte, and M. E. Rothenberg, “Expression and regulation of a disintegrin and metalloproteinase (ADAM) 8 in experimental asthma,” Am. J. Respir. Cell Mol. Biol. 31(3), 257–265 (2004).
[Crossref] [PubMed]

2001 (1)

J. M. Njoroge, L. B. Mitchell, M. Centola, D. Kastner, M. Raffeld, and J. L. Miller, “Characterization of viable autofluorescent macrophages among cultured peripheral blood mononuclear cells,” Cytometry 44(1), 38–44 (2001).
[Crossref] [PubMed]

1995 (1)

M. Monici, R. Pratesi, P. A. Bernabei, R. Caporale, P. R. Ferrini, A. C. Croce, P. Balzarini, and G. Bottiroli, “Natural fluorescence of white blood cells: spectroscopic and imaging study,” J. Photochem. Photobiol. B 30(1), 29–37 (1995).
[Crossref] [PubMed]

1992 (1)

A. N. Mayeno, K. J. Hamann, and G. J. Gleich, “Granule-associated flavin adenine dinucleotide (FAD) is responsible for eosinophil autofluorescence,” J. Leukoc. Biol. 51(2), 172–175 (1992).
[PubMed]

1979 (1)

R. C. Benson, R. A. Meyer, M. E. Zaruba, and G. M. McKhann, “Cellular autofluorescence--is it due to flavins?” J. Histochem. Cytochem. 27(1), 44–48 (1979).
[Crossref] [PubMed]

Aceto, N.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

Allard, W. J.

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

Alvarez, R. H.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

Balzarini, P.

M. Monici, R. Pratesi, P. A. Bernabei, R. Caporale, P. R. Ferrini, A. C. Croce, P. Balzarini, and G. Bottiroli, “Natural fluorescence of white blood cells: spectroscopic and imaging study,” J. Photochem. Photobiol. B 30(1), 29–37 (1995).
[Crossref] [PubMed]

Barber, T. A.

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Bardia, A.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

Barillé-Nion, S.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

Bartsch, J. W.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

G. Koller, U. Schlomann, P. Golfi, T. Ferdous, S. Naus, and J. W. Bartsch, “ADAM8/MS2/CD156, an Emerging Drug Target in the Treatment of Inflammatory and Invasive Pathologies,” Curr. Pharm. Des. 15(20), 2272–2281 (2009).
[Crossref] [PubMed]

Beck, K.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Benoy, I. H.

I. Van der Auwera, D. Peeters, I. H. Benoy, H. J. Elst, S. J. Van Laere, A. Prové, H. Maes, P. Huget, P. van Dam, P. B. Vermeulen, and L. Y. Dirix, “Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer,” Br. J. Cancer 102(2), 276–284 (2010).
[Crossref] [PubMed]

Benson, R. C.

R. C. Benson, R. A. Meyer, M. E. Zaruba, and G. M. McKhann, “Cellular autofluorescence--is it due to flavins?” J. Histochem. Cytochem. 27(1), 44–48 (1979).
[Crossref] [PubMed]

Bernabei, P. A.

M. Monici, R. Pratesi, P. A. Bernabei, R. Caporale, P. R. Ferrini, A. C. Croce, P. Balzarini, and G. Bottiroli, “Natural fluorescence of white blood cells: spectroscopic and imaging study,” J. Photochem. Photobiol. B 30(1), 29–37 (1995).
[Crossref] [PubMed]

Bottiroli, G.

M. Monici, R. Pratesi, P. A. Bernabei, R. Caporale, P. R. Ferrini, A. C. Croce, P. Balzarini, and G. Bottiroli, “Natural fluorescence of white blood cells: spectroscopic and imaging study,” J. Photochem. Photobiol. B 30(1), 29–37 (1995).
[Crossref] [PubMed]

Boutrus, S.

D. Hwu, S. Boutrus, C. Greiner, T. DiMeo, C. Kuperwasser, and I. Georgakoudi, “Assessment of the role of circulating breast cancer cells in tumor formation and metastatic potential using in vivo flow cytometry,” J. Biomed. Opt. 16(4), 040501 (2011).
[Crossref] [PubMed]

S. Boutrus, C. Greiner, D. Hwu, M. Chan, C. Kuperwasser, C. P. Lin, and I. Georgakoudi, “Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells,” J. Biomed. Opt. 12(2), 020507 (2007).
[Crossref] [PubMed]

Brannigan, B. W.

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

Budd, G. T.

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

Camps, O.

S. Markovic, B. Li, V. Pera, M. Sznaier, O. Camps, and M. Niedre, “A computer vision approach to rare cell in vivo fluorescence flow cytometry,” Cytometry A 83(12), 1113–1123 (2013).
[Crossref] [PubMed]

Caporale, R.

M. Monici, R. Pratesi, P. A. Bernabei, R. Caporale, P. R. Ferrini, A. C. Croce, P. Balzarini, and G. Bottiroli, “Natural fluorescence of white blood cells: spectroscopic and imaging study,” J. Photochem. Photobiol. B 30(1), 29–37 (1995).
[Crossref] [PubMed]

Centola, M.

J. M. Njoroge, L. B. Mitchell, M. Centola, D. Kastner, M. Raffeld, and J. L. Miller, “Characterization of viable autofluorescent macrophages among cultured peripheral blood mononuclear cells,” Cytometry 44(1), 38–44 (2001).
[Crossref] [PubMed]

Chan, M.

S. Boutrus, C. Greiner, D. Hwu, M. Chan, C. Kuperwasser, C. P. Lin, and I. Georgakoudi, “Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells,” J. Biomed. Opt. 12(2), 020507 (2007).
[Crossref] [PubMed]

Chen, P. I.

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Cheng, J.-X.

T. T. Le, T. B. Huff, and J.-X. Cheng, “Coherent anti-Stokes Raman scattering imaging of lipids in cancer metastasis,” BMC Cancer 9(1), 42 (2009).
[Crossref] [PubMed]

Chianese, D.

C. G. Rao, D. Chianese, G. V. Doyle, M. C. Miller, T. Russell, R. A. J. Sanders, and L. W. M. M. Terstappen, “Expression of epithelial cell adhesion molecule in carcinoma cells present in blood and primary and metastatic tumors,” Int. J. Oncol. 27(1), 49–57 (2005).
[PubMed]

Coith, C.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Conrad, C.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

Côté, D.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment,” Nature 435(7044), 969–973 (2005).
[Crossref] [PubMed]

Cristofanilli, M.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

Croce, A. C.

M. Monici, R. Pratesi, P. A. Bernabei, R. Caporale, P. R. Ferrini, A. C. Croce, P. Balzarini, and G. Bottiroli, “Natural fluorescence of white blood cells: spectroscopic and imaging study,” J. Photochem. Photobiol. B 30(1), 29–37 (1995).
[Crossref] [PubMed]

Dagg, Á.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

De Giorgi, U.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

De Laurentiis, M.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

De Placido, S.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

DiMeo, T.

D. Hwu, S. Boutrus, C. Greiner, T. DiMeo, C. Kuperwasser, and I. Georgakoudi, “Assessment of the role of circulating breast cancer cells in tumor formation and metastatic potential using in vivo flow cytometry,” J. Biomed. Opt. 16(4), 040501 (2011).
[Crossref] [PubMed]

Dirix, L. Y.

I. Van der Auwera, D. Peeters, I. H. Benoy, H. J. Elst, S. J. Van Laere, A. Prové, H. Maes, P. Huget, P. van Dam, P. B. Vermeulen, and L. Y. Dirix, “Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer,” Br. J. Cancer 102(2), 276–284 (2010).
[Crossref] [PubMed]

Donaldson, M. C.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

Doyle, G. V.

C. G. Rao, D. Chianese, G. V. Doyle, M. C. Miller, T. Russell, R. A. J. Sanders, and L. W. M. M. Terstappen, “Expression of epithelial cell adhesion molecule in carcinoma cells present in blood and primary and metastatic tumors,” Int. J. Oncol. 27(1), 49–57 (2005).
[PubMed]

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

Duffy, M. J.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

Dworzak, M. N.

K. Fišer, T. Sieger, A. Schumich, B. Wood, J. Irving, E. Mejstříková, and M. N. Dworzak, “Detection and monitoring of normal and leukemic cell populations with hierarchical clustering of flow cytometry data,” Cytometry A 81(1), 25–34 (2012).
[Crossref] [PubMed]

Ellis, M. J.

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

Elst, H. J.

I. Van der Auwera, D. Peeters, I. H. Benoy, H. J. Elst, S. J. Van Laere, A. Prové, H. Maes, P. Huget, P. van Dam, P. B. Vermeulen, and L. Y. Dirix, “Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer,” Br. J. Cancer 102(2), 276–284 (2010).
[Crossref] [PubMed]

Engstrom, A.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

Fachin, F.

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Fariss, R.

S. M. Pantanelli, Z. Li, R. Fariss, S. P. Mahesh, B. Liu, and R. B. Nussenblatt, “Differentiation of malignant B-lymphoma cells from normal and activated T-cell populations by their intrinsic autofluorescence,” Cancer Res. 69(11), 4911–4917 (2009).
[Crossref] [PubMed]

Ferdous, T.

G. Koller, U. Schlomann, P. Golfi, T. Ferdous, S. Naus, and J. W. Bartsch, “ADAM8/MS2/CD156, an Emerging Drug Target in the Treatment of Inflammatory and Invasive Pathologies,” Curr. Pharm. Des. 15(20), 2272–2281 (2009).
[Crossref] [PubMed]

Ferrini, P. R.

M. Monici, R. Pratesi, P. A. Bernabei, R. Caporale, P. R. Ferrini, A. C. Croce, P. Balzarini, and G. Bottiroli, “Natural fluorescence of white blood cells: spectroscopic and imaging study,” J. Photochem. Photobiol. B 30(1), 29–37 (1995).
[Crossref] [PubMed]

Fišer, K.

K. Fišer, T. Sieger, A. Schumich, B. Wood, J. Irving, E. Mejstříková, and M. N. Dworzak, “Detection and monitoring of normal and leukemic cell populations with hierarchical clustering of flow cytometry data,” Cytometry A 81(1), 25–34 (2012).
[Crossref] [PubMed]

Floyd, F. P.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Fritsche, H.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Fritsche, H. A.

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

Fulkerson, P. C.

N. E. King, N. Zimmermann, S. M. Pope, P. C. Fulkerson, N. M. Nikolaidis, A. Mishra, D. P. Witte, and M. E. Rothenberg, “Expression and regulation of a disintegrin and metalloproteinase (ADAM) 8 in experimental asthma,” Am. J. Respir. Cell Mol. Biol. 31(3), 257–265 (2004).
[Crossref] [PubMed]

Galanzha, E. I.

E. I. Galanzha, E. V. Shashkov, P. M. Spring, J. Y. Suen, and V. P. Zharov, “In vivo, noninvasive, label-free detection and eradication of circulating metastatic melanoma cells using two-color photoacoustic flow cytometry with a diode laser,” Cancer Res. 69(20), 7926–7934 (2009).
[Crossref] [PubMed]

Georgakoudi, I.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

D. Hwu, S. Boutrus, C. Greiner, T. DiMeo, C. Kuperwasser, and I. Georgakoudi, “Assessment of the role of circulating breast cancer cells in tumor formation and metastatic potential using in vivo flow cytometry,” J. Biomed. Opt. 16(4), 040501 (2011).
[Crossref] [PubMed]

C. Greiner, M. Hunter, F. Rius, P. Huang, and I. Georgakoudi, “Confocal backscattering-based detection of leukemic cells in flowing blood samples,” Cytometry A 79(10), 874–883 (2011).
[Crossref] [PubMed]

S. Boutrus, C. Greiner, D. Hwu, M. Chan, C. Kuperwasser, C. P. Lin, and I. Georgakoudi, “Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells,” J. Biomed. Opt. 12(2), 020507 (2007).
[Crossref] [PubMed]

I. Georgakoudi, N. Solban, J. Novak, W. L. Rice, X. Wei, T. Hasan, and C. P. Lin, “In vivo flow cytometry: a new method for enumerating circulating cancer cells advances in brief in vivo flow cytometry : a new method for enumerating circulating cancer cells,” Cancer Res. 64(15), 5044–5047 (2004).
[Crossref] [PubMed]

J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, “In vivo flow cytometer for real-time detection and quantification of circulating cells,” Opt. Lett. 29(1), 77–79 (2004).
[Crossref] [PubMed]

Gilman, A. J.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Giordano, A.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

Giuliano, M.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

Gleich, G. J.

A. N. Mayeno, K. J. Hamann, and G. J. Gleich, “Granule-associated flavin adenine dinucleotide (FAD) is responsible for eosinophil autofluorescence,” J. Leukoc. Biol. 51(2), 172–175 (1992).
[PubMed]

Golfi, P.

G. Koller, U. Schlomann, P. Golfi, T. Ferdous, S. Naus, and J. W. Bartsch, “ADAM8/MS2/CD156, an Emerging Drug Target in the Treatment of Inflammatory and Invasive Pathologies,” Curr. Pharm. Des. 15(20), 2272–2281 (2009).
[Crossref] [PubMed]

Gornet, T.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Greiner, C.

D. Hwu, S. Boutrus, C. Greiner, T. DiMeo, C. Kuperwasser, and I. Georgakoudi, “Assessment of the role of circulating breast cancer cells in tumor formation and metastatic potential using in vivo flow cytometry,” J. Biomed. Opt. 16(4), 040501 (2011).
[Crossref] [PubMed]

C. Greiner, M. Hunter, F. Rius, P. Huang, and I. Georgakoudi, “Confocal backscattering-based detection of leukemic cells in flowing blood samples,” Cytometry A 79(10), 874–883 (2011).
[Crossref] [PubMed]

S. Boutrus, C. Greiner, D. Hwu, M. Chan, C. Kuperwasser, C. P. Lin, and I. Georgakoudi, “Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells,” J. Biomed. Opt. 12(2), 020507 (2007).
[Crossref] [PubMed]

Haber, D. A.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Hamann, K. J.

A. N. Mayeno, K. J. Hamann, and G. J. Gleich, “Granule-associated flavin adenine dinucleotide (FAD) is responsible for eosinophil autofluorescence,” J. Leukoc. Biol. 51(2), 172–175 (1992).
[PubMed]

Hamza, B.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

Handy, B. C.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

Hasan, T.

I. Georgakoudi, N. Solban, J. Novak, W. L. Rice, X. Wei, T. Hasan, and C. P. Lin, “In vivo flow cytometry: a new method for enumerating circulating cancer cells advances in brief in vivo flow cytometry : a new method for enumerating circulating cancer cells,” Cancer Res. 64(15), 5044–5047 (2004).
[Crossref] [PubMed]

Hayes, D. F.

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

Hess, K. R.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

Hortobagyi, G. N.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

Hsu, C. H.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Huang, P.

C. Greiner, M. Hunter, F. Rius, P. Huang, and I. Georgakoudi, “Confocal backscattering-based detection of leukemic cells in flowing blood samples,” Cytometry A 79(10), 874–883 (2011).
[Crossref] [PubMed]

Huff, T. B.

T. T. Le, T. B. Huff, and J.-X. Cheng, “Coherent anti-Stokes Raman scattering imaging of lipids in cancer metastasis,” BMC Cancer 9(1), 42 (2009).
[Crossref] [PubMed]

Huget, P.

I. Van der Auwera, D. Peeters, I. H. Benoy, H. J. Elst, S. J. Van Laere, A. Prové, H. Maes, P. Huget, P. van Dam, P. B. Vermeulen, and L. Y. Dirix, “Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer,” Br. J. Cancer 102(2), 276–284 (2010).
[Crossref] [PubMed]

Hunter, M.

C. Greiner, M. Hunter, F. Rius, P. Huang, and I. Georgakoudi, “Confocal backscattering-based detection of leukemic cells in flowing blood samples,” Cytometry A 79(10), 874–883 (2011).
[Crossref] [PubMed]

Hwang, H.

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Hwu, D.

D. Hwu, S. Boutrus, C. Greiner, T. DiMeo, C. Kuperwasser, and I. Georgakoudi, “Assessment of the role of circulating breast cancer cells in tumor formation and metastatic potential using in vivo flow cytometry,” J. Biomed. Opt. 16(4), 040501 (2011).
[Crossref] [PubMed]

S. Boutrus, C. Greiner, D. Hwu, M. Chan, C. Kuperwasser, C. P. Lin, and I. Georgakoudi, “Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells,” J. Biomed. Opt. 12(2), 020507 (2007).
[Crossref] [PubMed]

Irimia, D.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Irving, J.

K. Fišer, T. Sieger, A. Schumich, B. Wood, J. Irving, E. Mejstříková, and M. N. Dworzak, “Detection and monitoring of normal and leukemic cell populations with hierarchical clustering of flow cytometry data,” Cytometry A 81(1), 25–34 (2012).
[Crossref] [PubMed]

Isselbacher, K. J.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Jackson, S.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Jänicke, F.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Janni, W.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Kapur, R.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Karabacak, N. M.

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Kastner, D.

J. M. Njoroge, L. B. Mitchell, M. Centola, D. Kastner, M. Raffeld, and J. L. Miller, “Characterization of viable autofluorescent macrophages among cultured peripheral blood mononuclear cells,” Cytometry 44(1), 38–44 (2001).
[Crossref] [PubMed]

King, N. E.

N. E. King, N. Zimmermann, S. M. Pope, P. C. Fulkerson, N. M. Nikolaidis, A. Mishra, D. P. Witte, and M. E. Rothenberg, “Expression and regulation of a disintegrin and metalloproteinase (ADAM) 8 in experimental asthma,” Am. J. Respir. Cell Mol. Biol. 31(3), 257–265 (2004).
[Crossref] [PubMed]

Kojic, N.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Koller, G.

G. Koller, U. Schlomann, P. Golfi, T. Ferdous, S. Naus, and J. W. Bartsch, “ADAM8/MS2/CD156, an Emerging Drug Target in the Treatment of Inflammatory and Invasive Pathologies,” Curr. Pharm. Des. 15(20), 2272–2281 (2009).
[Crossref] [PubMed]

Korir, G. K.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Kuperwasser, C.

D. Hwu, S. Boutrus, C. Greiner, T. DiMeo, C. Kuperwasser, and I. Georgakoudi, “Assessment of the role of circulating breast cancer cells in tumor formation and metastatic potential using in vivo flow cytometry,” J. Biomed. Opt. 16(4), 040501 (2011).
[Crossref] [PubMed]

S. Boutrus, C. Greiner, D. Hwu, M. Chan, C. Kuperwasser, C. P. Lin, and I. Georgakoudi, “Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells,” J. Biomed. Opt. 12(2), 020507 (2007).
[Crossref] [PubMed]

Le, T. T.

T. T. Le, T. B. Huff, and J.-X. Cheng, “Coherent anti-Stokes Raman scattering imaging of lipids in cancer metastasis,” BMC Cancer 9(1), 42 (2009).
[Crossref] [PubMed]

Lee, R. J.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Li, B.

S. Markovic, B. Li, V. Pera, M. Sznaier, O. Camps, and M. Niedre, “A computer vision approach to rare cell in vivo fluorescence flow cytometry,” Cytometry A 83(12), 1113–1123 (2013).
[Crossref] [PubMed]

Li, Z.

S. M. Pantanelli, Z. Li, R. Fariss, S. P. Mahesh, B. Liu, and R. B. Nussenblatt, “Differentiation of malignant B-lymphoma cells from normal and activated T-cell populations by their intrinsic autofluorescence,” Cancer Res. 69(11), 4911–4917 (2009).
[Crossref] [PubMed]

Lim, E. J.

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Lin, C. P.

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

S. Boutrus, C. Greiner, D. Hwu, M. Chan, C. Kuperwasser, C. P. Lin, and I. Georgakoudi, “Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells,” J. Biomed. Opt. 12(2), 020507 (2007).
[Crossref] [PubMed]

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment,” Nature 435(7044), 969–973 (2005).
[Crossref] [PubMed]

I. Georgakoudi, N. Solban, J. Novak, W. L. Rice, X. Wei, T. Hasan, and C. P. Lin, “In vivo flow cytometry: a new method for enumerating circulating cancer cells advances in brief in vivo flow cytometry : a new method for enumerating circulating cancer cells,” Cancer Res. 64(15), 5044–5047 (2004).
[Crossref] [PubMed]

J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, “In vivo flow cytometer for real-time detection and quantification of circulating cells,” Opt. Lett. 29(1), 77–79 (2004).
[Crossref] [PubMed]

Liu, B.

S. M. Pantanelli, Z. Li, R. Fariss, S. P. Mahesh, B. Liu, and R. B. Nussenblatt, “Differentiation of malignant B-lymphoma cells from normal and activated T-cell populations by their intrinsic autofluorescence,” Cancer Res. 69(11), 4911–4917 (2009).
[Crossref] [PubMed]

Lord, J. B.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Loussouarn, D.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

Luo, X.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

Luster, A. D.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment,” Nature 435(7044), 969–973 (2005).
[Crossref] [PubMed]

Maes, H.

I. Van der Auwera, D. Peeters, I. H. Benoy, H. J. Elst, S. J. Van Laere, A. Prové, H. Maes, P. Huget, P. van Dam, P. B. Vermeulen, and L. Y. Dirix, “Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer,” Br. J. Cancer 102(2), 276–284 (2010).
[Crossref] [PubMed]

Mahesh, S. P.

S. M. Pantanelli, Z. Li, R. Fariss, S. P. Mahesh, B. Liu, and R. B. Nussenblatt, “Differentiation of malignant B-lymphoma cells from normal and activated T-cell populations by their intrinsic autofluorescence,” Cancer Res. 69(11), 4911–4917 (2009).
[Crossref] [PubMed]

Maheswaran, S.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Markovic, S.

S. Markovic, B. Li, V. Pera, M. Sznaier, O. Camps, and M. Niedre, “A computer vision approach to rare cell in vivo fluorescence flow cytometry,” Cytometry A 83(12), 1113–1123 (2013).
[Crossref] [PubMed]

Martel, J. M.

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Matera, J.

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

Mayeno, A. N.

A. N. Mayeno, K. J. Hamann, and G. J. Gleich, “Granule-associated flavin adenine dinucleotide (FAD) is responsible for eosinophil autofluorescence,” J. Leukoc. Biol. 51(2), 172–175 (1992).
[PubMed]

McDermott, E. W.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

McGowan, P. M.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

McKhann, G. M.

R. C. Benson, R. A. Meyer, M. E. Zaruba, and G. M. McKhann, “Cellular autofluorescence--is it due to flavins?” J. Histochem. Cytochem. 27(1), 44–48 (1979).
[Crossref] [PubMed]

Means, T. K.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment,” Nature 435(7044), 969–973 (2005).
[Crossref] [PubMed]

Mego, M.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

Mehlen, P.

P. Mehlen and A. Puisieux, “Metastasis: a question of life or death,” Nat. Rev. Cancer 6(6), 449–458 (2006).
[Crossref] [PubMed]

Mejstríková, E.

K. Fišer, T. Sieger, A. Schumich, B. Wood, J. Irving, E. Mejstříková, and M. N. Dworzak, “Detection and monitoring of normal and leukemic cell populations with hierarchical clustering of flow cytometry data,” Cytometry A 81(1), 25–34 (2012).
[Crossref] [PubMed]

Meyer, R. A.

R. C. Benson, R. A. Meyer, M. E. Zaruba, and G. M. McKhann, “Cellular autofluorescence--is it due to flavins?” J. Histochem. Cytochem. 27(1), 44–48 (1979).
[Crossref] [PubMed]

Miller, J. L.

J. M. Njoroge, L. B. Mitchell, M. Centola, D. Kastner, M. Raffeld, and J. L. Miller, “Characterization of viable autofluorescent macrophages among cultured peripheral blood mononuclear cells,” Cytometry 44(1), 38–44 (2001).
[Crossref] [PubMed]

Miller, M. C.

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

C. G. Rao, D. Chianese, G. V. Doyle, M. C. Miller, T. Russell, R. A. J. Sanders, and L. W. M. M. Terstappen, “Expression of epithelial cell adhesion molecule in carcinoma cells present in blood and primary and metastatic tumors,” Int. J. Oncol. 27(1), 49–57 (2005).
[PubMed]

Mineva, N. D.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

Mishra, A.

N. E. King, N. Zimmermann, S. M. Pope, P. C. Fulkerson, N. M. Nikolaidis, A. Mishra, D. P. Witte, and M. E. Rothenberg, “Expression and regulation of a disintegrin and metalloproteinase (ADAM) 8 in experimental asthma,” Am. J. Respir. Cell Mol. Biol. 31(3), 257–265 (2004).
[Crossref] [PubMed]

Mitchell, L. B.

J. M. Njoroge, L. B. Mitchell, M. Centola, D. Kastner, M. Raffeld, and J. L. Miller, “Characterization of viable autofluorescent macrophages among cultured peripheral blood mononuclear cells,” Cytometry 44(1), 38–44 (2001).
[Crossref] [PubMed]

Miyamoto, D. T.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Monici, M.

M. Monici, R. Pratesi, P. A. Bernabei, R. Caporale, P. R. Ferrini, A. C. Croce, P. Balzarini, and G. Bottiroli, “Natural fluorescence of white blood cells: spectroscopic and imaging study,” J. Photochem. Photobiol. B 30(1), 29–37 (1995).
[Crossref] [PubMed]

Morgan, B.

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Müller, V.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Nagrath, S.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Naus, S.

G. Koller, U. Schlomann, P. Golfi, T. Ferdous, S. Naus, and J. W. Bartsch, “ADAM8/MS2/CD156, an Emerging Drug Target in the Treatment of Inflammatory and Invasive Pathologies,” Curr. Pharm. Des. 15(20), 2272–2281 (2009).
[Crossref] [PubMed]

Niedre, M.

S. Markovic, B. Li, V. Pera, M. Sznaier, O. Camps, and M. Niedre, “A computer vision approach to rare cell in vivo fluorescence flow cytometry,” Cytometry A 83(12), 1113–1123 (2013).
[Crossref] [PubMed]

Nikolaidis, N. M.

N. E. King, N. Zimmermann, S. M. Pope, P. C. Fulkerson, N. M. Nikolaidis, A. Mishra, D. P. Witte, and M. E. Rothenberg, “Expression and regulation of a disintegrin and metalloproteinase (ADAM) 8 in experimental asthma,” Am. J. Respir. Cell Mol. Biol. 31(3), 257–265 (2004).
[Crossref] [PubMed]

Njoroge, J. M.

J. M. Njoroge, L. B. Mitchell, M. Centola, D. Kastner, M. Raffeld, and J. L. Miller, “Characterization of viable autofluorescent macrophages among cultured peripheral blood mononuclear cells,” Cytometry 44(1), 38–44 (2001).
[Crossref] [PubMed]

Novak, J.

J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, “In vivo flow cytometer for real-time detection and quantification of circulating cells,” Opt. Lett. 29(1), 77–79 (2004).
[Crossref] [PubMed]

I. Georgakoudi, N. Solban, J. Novak, W. L. Rice, X. Wei, T. Hasan, and C. P. Lin, “In vivo flow cytometry: a new method for enumerating circulating cancer cells advances in brief in vivo flow cytometry : a new method for enumerating circulating cancer cells,” Cancer Res. 64(15), 5044–5047 (2004).
[Crossref] [PubMed]

Nussenblatt, R. B.

S. M. Pantanelli, Z. Li, R. Fariss, S. P. Mahesh, B. Liu, and R. B. Nussenblatt, “Differentiation of malignant B-lymphoma cells from normal and activated T-cell populations by their intrinsic autofluorescence,” Cancer Res. 69(11), 4911–4917 (2009).
[Crossref] [PubMed]

Ozkumur, E.

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Pai, V.

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Pantanelli, S. M.

S. M. Pantanelli, Z. Li, R. Fariss, S. P. Mahesh, B. Liu, and R. B. Nussenblatt, “Differentiation of malignant B-lymphoma cells from normal and activated T-cell populations by their intrinsic autofluorescence,” Cancer Res. 69(11), 4911–4917 (2009).
[Crossref] [PubMed]

Pantel, K.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Parsons, M.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

Peeters, D.

I. Van der Auwera, D. Peeters, I. H. Benoy, H. J. Elst, S. J. Van Laere, A. Prové, H. Maes, P. Huget, P. van Dam, P. B. Vermeulen, and L. Y. Dirix, “Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer,” Br. J. Cancer 102(2), 276–284 (2010).
[Crossref] [PubMed]

Pely, A.

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

Pera, V.

S. Markovic, B. Li, V. Pera, M. Sznaier, O. Camps, and M. Niedre, “A computer vision approach to rare cell in vivo fluorescence flow cytometry,” Cytometry A 83(12), 1113–1123 (2013).
[Crossref] [PubMed]

Polmear, M.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

Pope, S. M.

N. E. King, N. Zimmermann, S. M. Pope, P. C. Fulkerson, N. M. Nikolaidis, A. Mishra, D. P. Witte, and M. E. Rothenberg, “Expression and regulation of a disintegrin and metalloproteinase (ADAM) 8 in experimental asthma,” Am. J. Respir. Cell Mol. Biol. 31(3), 257–265 (2004).
[Crossref] [PubMed]

Pratesi, R.

M. Monici, R. Pratesi, P. A. Bernabei, R. Caporale, P. R. Ferrini, A. C. Croce, P. Balzarini, and G. Bottiroli, “Natural fluorescence of white blood cells: spectroscopic and imaging study,” J. Photochem. Photobiol. B 30(1), 29–37 (1995).
[Crossref] [PubMed]

Prossin, A.

Prové, A.

I. Van der Auwera, D. Peeters, I. H. Benoy, H. J. Elst, S. J. Van Laere, A. Prové, H. Maes, P. Huget, P. van Dam, P. B. Vermeulen, and L. Y. Dirix, “Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer,” Br. J. Cancer 102(2), 276–284 (2010).
[Crossref] [PubMed]

Puisieux, A.

P. Mehlen and A. Puisieux, “Metastasis: a question of life or death,” Nat. Rev. Cancer 6(6), 449–458 (2006).
[Crossref] [PubMed]

Rack, B.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Raffeld, M.

J. M. Njoroge, L. B. Mitchell, M. Centola, D. Kastner, M. Raffeld, and J. L. Miller, “Characterization of viable autofluorescent macrophages among cultured peripheral blood mononuclear cells,” Cytometry 44(1), 38–44 (2001).
[Crossref] [PubMed]

Ramaswamy, S.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

Rao, C. G.

C. G. Rao, D. Chianese, G. V. Doyle, M. C. Miller, T. Russell, R. A. J. Sanders, and L. W. M. M. Terstappen, “Expression of epithelial cell adhesion molecule in carcinoma cells present in blood and primary and metastatic tumors,” Int. J. Oncol. 27(1), 49–57 (2005).
[PubMed]

Rau, T.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Reuben, J. M.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

Rice, W. L.

I. Georgakoudi, N. Solban, J. Novak, W. L. Rice, X. Wei, T. Hasan, and C. P. Lin, “In vivo flow cytometry: a new method for enumerating circulating cancer cells advances in brief in vivo flow cytometry : a new method for enumerating circulating cancer cells,” Cancer Res. 64(15), 5044–5047 (2004).
[Crossref] [PubMed]

Riethdorf, S.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Rius, F.

C. Greiner, M. Hunter, F. Rius, P. Huang, and I. Georgakoudi, “Confocal backscattering-based detection of leukemic cells in flowing blood samples,” Cytometry A 79(10), 874–883 (2011).
[Crossref] [PubMed]

Romagnoli, M.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

Rothenberg, M. E.

N. E. King, N. Zimmermann, S. M. Pope, P. C. Fulkerson, N. M. Nikolaidis, A. Mishra, D. P. Witte, and M. E. Rothenberg, “Expression and regulation of a disintegrin and metalloproteinase (ADAM) 8 in experimental asthma,” Am. J. Respir. Cell Mol. Biol. 31(3), 257–265 (2004).
[Crossref] [PubMed]

Rothenberg, S. M.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Runnels, J. M.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment,” Nature 435(7044), 969–973 (2005).
[Crossref] [PubMed]

Russell, T.

C. G. Rao, D. Chianese, G. V. Doyle, M. C. Miller, T. Russell, R. A. J. Sanders, and L. W. M. M. Terstappen, “Expression of epithelial cell adhesion molecule in carcinoma cells present in blood and primary and metastatic tumors,” Int. J. Oncol. 27(1), 49–57 (2005).
[PubMed]

Sanders, R. A. J.

C. G. Rao, D. Chianese, G. V. Doyle, M. C. Miller, T. Russell, R. A. J. Sanders, and L. W. M. M. Terstappen, “Expression of epithelial cell adhesion molecule in carcinoma cells present in blood and primary and metastatic tumors,” Int. J. Oncol. 27(1), 49–57 (2005).
[PubMed]

Sarioglu, A. F.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

Scadden, D. T.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment,” Nature 435(7044), 969–973 (2005).
[Crossref] [PubMed]

Schindlbeck, C.

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Schlomann, U.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

G. Koller, U. Schlomann, P. Golfi, T. Ferdous, S. Naus, and J. W. Bartsch, “ADAM8/MS2/CD156, an Emerging Drug Target in the Treatment of Inflammatory and Invasive Pathologies,” Curr. Pharm. Des. 15(20), 2272–2281 (2009).
[Crossref] [PubMed]

Schumich, A.

K. Fišer, T. Sieger, A. Schumich, B. Wood, J. Irving, E. Mejstříková, and M. N. Dworzak, “Detection and monitoring of normal and leukemic cell populations with hierarchical clustering of flow cytometry data,” Cytometry A 81(1), 25–34 (2012).
[Crossref] [PubMed]

Sequist, L. V.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Shah, A. M.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Shashkov, E. V.

E. I. Galanzha, E. V. Shashkov, P. M. Spring, J. Y. Suen, and V. P. Zharov, “In vivo, noninvasive, label-free detection and eradication of circulating metastatic melanoma cells using two-color photoacoustic flow cytometry with a diode laser,” Cancer Res. 69(20), 7926–7934 (2009).
[Crossref] [PubMed]

Shioda, T.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

Sieger, T.

K. Fišer, T. Sieger, A. Schumich, B. Wood, J. Irving, E. Mejstříková, and M. N. Dworzak, “Detection and monitoring of normal and leukemic cell populations with hierarchical clustering of flow cytometry data,” Cytometry A 81(1), 25–34 (2012).
[Crossref] [PubMed]

Sipkins, D. A.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment,” Nature 435(7044), 969–973 (2005).
[Crossref] [PubMed]

Smas, M. E.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Smith, K.

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Solban, N.

I. Georgakoudi, N. Solban, J. Novak, W. L. Rice, X. Wei, T. Hasan, and C. P. Lin, “In vivo flow cytometry: a new method for enumerating circulating cancer cells advances in brief in vivo flow cytometry : a new method for enumerating circulating cancer cells,” Cancer Res. 64(15), 5044–5047 (2004).
[Crossref] [PubMed]

Sonenshein, G. E.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

Spencer, J. A.

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

Spring, P. M.

E. I. Galanzha, E. V. Shashkov, P. M. Spring, J. Y. Suen, and V. P. Zharov, “In vivo, noninvasive, label-free detection and eradication of circulating metastatic melanoma cells using two-color photoacoustic flow cytometry with a diode laser,” Cancer Res. 69(20), 7926–7934 (2009).
[Crossref] [PubMed]

Springer, S.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Spuhler, P. S.

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Srinivasan, S.

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

Stopeck, A.

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

Stott, S. L.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Suen, J. Y.

E. I. Galanzha, E. V. Shashkov, P. M. Spring, J. Y. Suen, and V. P. Zharov, “In vivo, noninvasive, label-free detection and eradication of circulating metastatic melanoma cells using two-color photoacoustic flow cytometry with a diode laser,” Cancer Res. 69(20), 7926–7934 (2009).
[Crossref] [PubMed]

Sundaresan, T. K.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

Sznaier, M.

S. Markovic, B. Li, V. Pera, M. Sznaier, O. Camps, and M. Niedre, “A computer vision approach to rare cell in vivo fluorescence flow cytometry,” Cytometry A 83(12), 1113–1123 (2013).
[Crossref] [PubMed]

Terstappen, L. W.

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

Terstappen, L. W. M. M.

C. G. Rao, D. Chianese, G. V. Doyle, M. C. Miller, T. Russell, R. A. J. Sanders, and L. W. M. M. Terstappen, “Expression of epithelial cell adhesion molecule in carcinoma cells present in blood and primary and metastatic tumors,” Int. J. Oncol. 27(1), 49–57 (2005).
[PubMed]

Ting, D. T.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

Toner, M.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Trautwein, J.

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Tsukrov, D. I.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Ueno, N. T.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

Valastyan, S.

S. Valastyan and R. A. Weinberg, “Tumor metastasis: molecular insights and evolving paradigms,” Cell 147(2), 275–292 (2011).
[Crossref] [PubMed]

Valero, V.

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

van Dam, P.

I. Van der Auwera, D. Peeters, I. H. Benoy, H. J. Elst, S. J. Van Laere, A. Prové, H. Maes, P. Huget, P. van Dam, P. B. Vermeulen, and L. Y. Dirix, “Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer,” Br. J. Cancer 102(2), 276–284 (2010).
[Crossref] [PubMed]

Van der Auwera, I.

I. Van der Auwera, D. Peeters, I. H. Benoy, H. J. Elst, S. J. Van Laere, A. Prové, H. Maes, P. Huget, P. van Dam, P. B. Vermeulen, and L. Y. Dirix, “Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer,” Br. J. Cancer 102(2), 276–284 (2010).
[Crossref] [PubMed]

Van Laere, S. J.

I. Van der Auwera, D. Peeters, I. H. Benoy, H. J. Elst, S. J. Van Laere, A. Prové, H. Maes, P. Huget, P. van Dam, P. B. Vermeulen, and L. Y. Dirix, “Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer,” Br. J. Cancer 102(2), 276–284 (2010).
[Crossref] [PubMed]

Vermeulen, P. B.

I. Van der Auwera, D. Peeters, I. H. Benoy, H. J. Elst, S. J. Van Laere, A. Prové, H. Maes, P. Huget, P. van Dam, P. B. Vermeulen, and L. Y. Dirix, “Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer,” Br. J. Cancer 102(2), 276–284 (2010).
[Crossref] [PubMed]

Waltman, B. A.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Wei, X.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment,” Nature 435(7044), 969–973 (2005).
[Crossref] [PubMed]

I. Georgakoudi, N. Solban, J. Novak, W. L. Rice, X. Wei, T. Hasan, and C. P. Lin, “In vivo flow cytometry: a new method for enumerating circulating cancer cells advances in brief in vivo flow cytometry : a new method for enumerating circulating cancer cells,” Cancer Res. 64(15), 5044–5047 (2004).
[Crossref] [PubMed]

J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, “In vivo flow cytometer for real-time detection and quantification of circulating cells,” Opt. Lett. 29(1), 77–79 (2004).
[Crossref] [PubMed]

Weinberg, R. A.

S. Valastyan and R. A. Weinberg, “Tumor metastasis: molecular insights and evolving paradigms,” Cell 147(2), 275–292 (2011).
[Crossref] [PubMed]

Winokur, D.

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Witte, D. P.

N. E. King, N. Zimmermann, S. M. Pope, P. C. Fulkerson, N. M. Nikolaidis, A. Mishra, D. P. Witte, and M. E. Rothenberg, “Expression and regulation of a disintegrin and metalloproteinase (ADAM) 8 in experimental asthma,” Am. J. Respir. Cell Mol. Biol. 31(3), 257–265 (2004).
[Crossref] [PubMed]

Wittner, B. S.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

Wood, B.

K. Fišer, T. Sieger, A. Schumich, B. Wood, J. Irving, E. Mejstříková, and M. N. Dworzak, “Detection and monitoring of normal and leukemic cell populations with hierarchical clustering of flow cytometry data,” Cytometry A 81(1), 25–34 (2012).
[Crossref] [PubMed]

Wu, J. W.

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment,” Nature 435(7044), 969–973 (2005).
[Crossref] [PubMed]

Yang, J.

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Yu, M.

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Zaruba, M. E.

R. C. Benson, R. A. Meyer, M. E. Zaruba, and G. M. McKhann, “Cellular autofluorescence--is it due to flavins?” J. Histochem. Cytochem. 27(1), 44–48 (1979).
[Crossref] [PubMed]

Zeinali, M.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

Zharov, V. P.

E. I. Galanzha, E. V. Shashkov, P. M. Spring, J. Y. Suen, and V. P. Zharov, “In vivo, noninvasive, label-free detection and eradication of circulating metastatic melanoma cells using two-color photoacoustic flow cytometry with a diode laser,” Cancer Res. 69(20), 7926–7934 (2009).
[Crossref] [PubMed]

Zhu, H.

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

Zimmermann, N.

N. E. King, N. Zimmermann, S. M. Pope, P. C. Fulkerson, N. M. Nikolaidis, A. Mishra, D. P. Witte, and M. E. Rothenberg, “Expression and regulation of a disintegrin and metalloproteinase (ADAM) 8 in experimental asthma,” Am. J. Respir. Cell Mol. Biol. 31(3), 257–265 (2004).
[Crossref] [PubMed]

Am. J. Respir. Cell Mol. Biol. (1)

N. E. King, N. Zimmermann, S. M. Pope, P. C. Fulkerson, N. M. Nikolaidis, A. Mishra, D. P. Witte, and M. E. Rothenberg, “Expression and regulation of a disintegrin and metalloproteinase (ADAM) 8 in experimental asthma,” Am. J. Respir. Cell Mol. Biol. 31(3), 257–265 (2004).
[Crossref] [PubMed]

BMC Cancer (1)

T. T. Le, T. B. Huff, and J.-X. Cheng, “Coherent anti-Stokes Raman scattering imaging of lipids in cancer metastasis,” BMC Cancer 9(1), 42 (2009).
[Crossref] [PubMed]

Br. J. Cancer (1)

I. Van der Auwera, D. Peeters, I. H. Benoy, H. J. Elst, S. J. Van Laere, A. Prové, H. Maes, P. Huget, P. van Dam, P. B. Vermeulen, and L. Y. Dirix, “Circulating tumour cell detection: a direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer,” Br. J. Cancer 102(2), 276–284 (2010).
[Crossref] [PubMed]

Breast Cancer Res. (1)

M. Giuliano, A. Giordano, S. Jackson, K. R. Hess, U. De Giorgi, M. Mego, B. C. Handy, N. T. Ueno, R. H. Alvarez, M. De Laurentiis, S. De Placido, V. Valero, G. N. Hortobagyi, J. M. Reuben, and M. Cristofanilli, “Circulating tumor cells as prognostic and predictive markers in metastatic breast cancer patients receiving first-line systemic treatment,” Breast Cancer Res. 13(3), R67 (2011).
[Crossref] [PubMed]

Cancer Res. (3)

E. I. Galanzha, E. V. Shashkov, P. M. Spring, J. Y. Suen, and V. P. Zharov, “In vivo, noninvasive, label-free detection and eradication of circulating metastatic melanoma cells using two-color photoacoustic flow cytometry with a diode laser,” Cancer Res. 69(20), 7926–7934 (2009).
[Crossref] [PubMed]

I. Georgakoudi, N. Solban, J. Novak, W. L. Rice, X. Wei, T. Hasan, and C. P. Lin, “In vivo flow cytometry: a new method for enumerating circulating cancer cells advances in brief in vivo flow cytometry : a new method for enumerating circulating cancer cells,” Cancer Res. 64(15), 5044–5047 (2004).
[Crossref] [PubMed]

S. M. Pantanelli, Z. Li, R. Fariss, S. P. Mahesh, B. Liu, and R. B. Nussenblatt, “Differentiation of malignant B-lymphoma cells from normal and activated T-cell populations by their intrinsic autofluorescence,” Cancer Res. 69(11), 4911–4917 (2009).
[Crossref] [PubMed]

Cell (2)

S. Valastyan and R. A. Weinberg, “Tumor metastasis: molecular insights and evolving paradigms,” Cell 147(2), 275–292 (2011).
[Crossref] [PubMed]

N. Aceto, A. Bardia, D. T. Miyamoto, M. C. Donaldson, B. S. Wittner, J. A. Spencer, M. Yu, A. Pely, A. Engstrom, H. Zhu, B. W. Brannigan, R. Kapur, S. L. Stott, T. Shioda, S. Ramaswamy, D. T. Ting, C. P. Lin, M. Toner, D. A. Haber, and S. Maheswaran, “Circulating Tumor Cell Clusters Are Oligoclonal Precursors of Breast Cancer Metastasis,” Cell 158(5), 1110–1122 (2014).
[Crossref] [PubMed]

Clin. Cancer Res. (1)

S. Riethdorf, H. Fritsche, V. Müller, T. Rau, C. Schindlbeck, B. Rack, W. Janni, C. Coith, K. Beck, F. Jänicke, S. Jackson, T. Gornet, M. Cristofanilli, and K. Pantel, “Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system,” Clin. Cancer Res. 13(3), 920–928 (2007).
[Crossref] [PubMed]

Curr. Pharm. Des. (1)

G. Koller, U. Schlomann, P. Golfi, T. Ferdous, S. Naus, and J. W. Bartsch, “ADAM8/MS2/CD156, an Emerging Drug Target in the Treatment of Inflammatory and Invasive Pathologies,” Curr. Pharm. Des. 15(20), 2272–2281 (2009).
[Crossref] [PubMed]

Cytometry (1)

J. M. Njoroge, L. B. Mitchell, M. Centola, D. Kastner, M. Raffeld, and J. L. Miller, “Characterization of viable autofluorescent macrophages among cultured peripheral blood mononuclear cells,” Cytometry 44(1), 38–44 (2001).
[Crossref] [PubMed]

Cytometry A (3)

K. Fišer, T. Sieger, A. Schumich, B. Wood, J. Irving, E. Mejstříková, and M. N. Dworzak, “Detection and monitoring of normal and leukemic cell populations with hierarchical clustering of flow cytometry data,” Cytometry A 81(1), 25–34 (2012).
[Crossref] [PubMed]

C. Greiner, M. Hunter, F. Rius, P. Huang, and I. Georgakoudi, “Confocal backscattering-based detection of leukemic cells in flowing blood samples,” Cytometry A 79(10), 874–883 (2011).
[Crossref] [PubMed]

S. Markovic, B. Li, V. Pera, M. Sznaier, O. Camps, and M. Niedre, “A computer vision approach to rare cell in vivo fluorescence flow cytometry,” Cytometry A 83(12), 1113–1123 (2013).
[Crossref] [PubMed]

EMBO Mol. Med. (1)

M. Romagnoli, N. D. Mineva, M. Polmear, C. Conrad, S. Srinivasan, D. Loussouarn, S. Barillé-Nion, I. Georgakoudi, Á. Dagg, E. W. McDermott, M. J. Duffy, P. M. McGowan, U. Schlomann, M. Parsons, J. W. Bartsch, and G. E. Sonenshein, “ADAM8 expression in invasive breast cancer promotes tumor dissemination and metastasis,” EMBO Mol. Med. 6(2), 278–294 (2014).
[PubMed]

Int. J. Oncol. (1)

C. G. Rao, D. Chianese, G. V. Doyle, M. C. Miller, T. Russell, R. A. J. Sanders, and L. W. M. M. Terstappen, “Expression of epithelial cell adhesion molecule in carcinoma cells present in blood and primary and metastatic tumors,” Int. J. Oncol. 27(1), 49–57 (2005).
[PubMed]

J. Biomed. Opt. (2)

S. Boutrus, C. Greiner, D. Hwu, M. Chan, C. Kuperwasser, C. P. Lin, and I. Georgakoudi, “Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells,” J. Biomed. Opt. 12(2), 020507 (2007).
[Crossref] [PubMed]

D. Hwu, S. Boutrus, C. Greiner, T. DiMeo, C. Kuperwasser, and I. Georgakoudi, “Assessment of the role of circulating breast cancer cells in tumor formation and metastatic potential using in vivo flow cytometry,” J. Biomed. Opt. 16(4), 040501 (2011).
[Crossref] [PubMed]

J. Clin. Oncol. (1)

M. Cristofanilli, D. F. Hayes, G. T. Budd, M. J. Ellis, A. Stopeck, J. M. Reuben, G. V. Doyle, J. Matera, W. J. Allard, M. C. Miller, H. A. Fritsche, G. N. Hortobagyi, and L. W. Terstappen, “Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer,” J. Clin. Oncol. 23(7), 1420–1430 (2005).
[Crossref] [PubMed]

J. Histochem. Cytochem. (1)

R. C. Benson, R. A. Meyer, M. E. Zaruba, and G. M. McKhann, “Cellular autofluorescence--is it due to flavins?” J. Histochem. Cytochem. 27(1), 44–48 (1979).
[Crossref] [PubMed]

J. Leukoc. Biol. (1)

A. N. Mayeno, K. J. Hamann, and G. J. Gleich, “Granule-associated flavin adenine dinucleotide (FAD) is responsible for eosinophil autofluorescence,” J. Leukoc. Biol. 51(2), 172–175 (1992).
[PubMed]

J. Photochem. Photobiol. B (1)

M. Monici, R. Pratesi, P. A. Bernabei, R. Caporale, P. R. Ferrini, A. C. Croce, P. Balzarini, and G. Bottiroli, “Natural fluorescence of white blood cells: spectroscopic and imaging study,” J. Photochem. Photobiol. B 30(1), 29–37 (1995).
[Crossref] [PubMed]

Nat. Methods (1)

A. F. Sarioglu, N. Aceto, N. Kojic, M. C. Donaldson, M. Zeinali, B. Hamza, A. Engstrom, H. Zhu, T. K. Sundaresan, D. T. Miyamoto, X. Luo, A. Bardia, B. S. Wittner, S. Ramaswamy, T. Shioda, D. T. Ting, S. L. Stott, R. Kapur, S. Maheswaran, D. A. Haber, and M. Toner, “A microfluidic device for label-free, physical capture of circulating tumor cell clusters,” Nat. Methods 12(7), 685–691 (2015).
[Crossref] [PubMed]

Nat. Protoc. (1)

N. M. Karabacak, P. S. Spuhler, F. Fachin, E. J. Lim, V. Pai, E. Ozkumur, J. M. Martel, N. Kojic, K. Smith, P. I. Chen, J. Yang, H. Hwang, B. Morgan, J. Trautwein, T. A. Barber, S. L. Stott, S. Maheswaran, R. Kapur, D. A. Haber, and M. Toner, “Microfluidic, marker-free isolation of circulating tumor cells from blood samples,” Nat. Protoc. 9(3), 694–710 (2014).
[Crossref] [PubMed]

Nat. Rev. Cancer (1)

P. Mehlen and A. Puisieux, “Metastasis: a question of life or death,” Nat. Rev. Cancer 6(6), 449–458 (2006).
[Crossref] [PubMed]

Nature (1)

D. A. Sipkins, X. Wei, J. W. Wu, J. M. Runnels, D. Côté, T. K. Means, A. D. Luster, D. T. Scadden, and C. P. Lin, “In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment,” Nature 435(7044), 969–973 (2005).
[Crossref] [PubMed]

Opt. Lett. (1)

Proc. Natl. Acad. Sci. U.S.A. (1)

S. L. Stott, C. H. Hsu, D. I. Tsukrov, M. Yu, D. T. Miyamoto, B. A. Waltman, S. M. Rothenberg, A. M. Shah, M. E. Smas, G. K. Korir, F. P. Floyd, A. J. Gilman, J. B. Lord, D. Winokur, S. Springer, D. Irimia, S. Nagrath, L. V. Sequist, R. J. Lee, K. J. Isselbacher, S. Maheswaran, D. A. Haber, and M. Toner, “Isolation of circulating tumor cells using a microvortex-generating herringbone-chip,” Proc. Natl. Acad. Sci. U.S.A. 107(43), 18392–18397 (2010).
[Crossref] [PubMed]

Other (2)

L. Sherwood, Human Physiology: From Cells to Systems, 9th ed. (Cengage Learning, Boston,MA, 2015), p. 393.

R. F. Woolson, Statistical Methods for the Analysis of Biomedical Data, Wiley Series in Probability and Mathematical Statistics (Wiley, New York, NY, 1987).

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Figures (5)

Fig. 1
Fig. 1 Representative raw data time traces of a 2λ event (A), a 3λ event (B), a 4λ event (C), and a saturated and large FWHM 4λ event (D). The saturation output from the PMTs is 10V. For each panel, the top trace contains the scattering from the 405nm (black), 488nm (blue), and 633nm (red) scattering channels, the second and bottom traces contain the green fluorescence(green) and cumulative intensity(magenta) traces respectively.
Fig. 2
Fig. 2 NC mice were found to have a small green autofluorescent population. Blood from the AA8 mice had a higher proportion of green fluorescent cells compared to both the ISO and NC groups (*p<0.01). ISO and NC mice did not have statistically significant differences in the proportion of detected green fluorescent cells.
Fig. 3
Fig. 3 Box and whisker plots for the distribution of parameters of non-fluorescent cells in grey, and fluorescent cells in black for all treatment groups. Panels A through E are in log scale while panel F is in linear scale. Fluorescent cells for each treatment groups had higher mean-ranks for the cumulative intensities compared to their corresponding non-fluorescent cells (*p<0.01).
Fig. 4
Fig. 4 The Mahalanobis distance calculated for each fluorescent event detected based on the values of the peak intensity ratios, cumulative intensity and FWHM for NC (light grey), isotype (grey), and anti-ADAM8 treated mice (black).
Fig. 5
Fig. 5 A 4 dimensional plot of the derived parameters reveals that fluorescent outliers (red) occupy a region with high 405/633, 488/633, 405/488, and increased FWHM scattering. Each axis is in log scale. The FWHM intensity is linearly signified by marker size. The 14 extreme outliers are plotted in red.

Tables (1)

Tables Icon

Table 1 Characteristics of Fluorescent Outliers and Fluorescent Non-outliers

Metrics