Abstract

Optical mesoscopy extends the capabilities of biological visualization beyond the limited penetration depth achieved by microscopy. However, imaging of opaque organisms or tissues larger than a few hundred micrometers requires invasive tissue sectioning or chemical treatment of the specimen for clearing photon scattering, an invasive process that is regardless limited with depth. We developed previously unreported broadband optoacoustic mesoscopy as a tomographic modality to enable imaging of optical contrast through several millimeters of tissue, without the need for chemical treatment of tissues. We show that the unique combination of three-dimensional projections over a broad 500 kHz–40 MHz frequency range combined with multi-wavelength illumination is necessary to render broadband multispectral optoacoustic mesoscopy (2B-MSOM) superior to previous optical or optoacoustic mesoscopy implementations.

© 2015 Optical Society of America

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References

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    [Crossref] [PubMed]
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    [Crossref] [PubMed]
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2014 (4)

M. Dobosz, V. Ntziachristos, W. Scheuer, and S. Strobel, “Multispectral Fluorescence Ultramicroscopy: Three-Dimensional Visualization and Automatic Quantification of Tumor Morphology, Drug Penetration, and Antiangiogenic Treatment Response,” Neoplasia 16(1), 1–13 (2014).
[Crossref] [PubMed]

S. Tzoumas, N. Deliolanis, S. Morscher, and V. Ntziachristos, “Unmixing Molecular Agents From Absorbing Tissue in Multispectral Optoacoustic Tomography,” IEEE Trans. Med. Imaging 33(1), 48–60 (2014).
[Crossref]

M. A. Araque Caballero, J. Gateau, X.-L. Dean-Ben, and V. Ntziachristos, “Model-based optoacoustic image reconstruction of large three-dimensional tomographic datasets acquired with an array of directional detectors,” IEEE Trans. Med. Imaging 33(2), 433–443 (2014).
[Crossref] [PubMed]

A. Rosenthal, S. Kellnberger, D. Bozhko, A. Chekkoury, M. Omar, D. Razansky, and V. Ntziachristos, “Sensitive interferometric detection of ultrasound for minimally invasive clinical imaging applications,” Laser Photon. Rev. 8(3), 450–457 (2014).
[Crossref]

2013 (3)

J. Gateau, A. Chekkoury, and V. Ntziachristos, “Ultra-wideband three-dimensional optoacoustic tomography,” Opt. Lett. 38(22), 4671–4674 (2013).
[Crossref] [PubMed]

J. Gateau, A. Chekkoury, and V. Ntziachristos, “High-resolution optoacoustic mesoscopy with a 24 MHz multidetector translate-rotate scanner,” J. Biomed. Opt. 18(10), 106005 (2013).
[Crossref] [PubMed]

J. Gateau, M. A. A. Caballero, A. Dima, and V. Ntziachristos, “Three-dimensional optoacoustic tomography using a conventional ultrasound linear detector array: Whole-body tomographic system for small animals,” Med. Phys. 40(1), 013302 (2013).
[Crossref] [PubMed]

2012 (3)

E. Herzog, A. Taruttis, N. Beziere, A. A. Lutich, D. Razansky, and V. Ntziachristos, “Optical Imaging of Cancer Heterogeneity with Multispectral Optoacoustic Tomography,” Radiology 263(2), 461–468 (2012).
[Crossref] [PubMed]

L. V. Wang and S. Hu, “Photoacoustic tomography: in vivo imaging from organelles to organs,” Science 335(6075), 1458–1462 (2012).
[Crossref] [PubMed]

A. Taruttis, S. Morscher, N. C. Burton, D. Razansky, and V. Ntziachristos, “Fast Multispectral Optoacoustic Tomography (MSOT) for Dynamic Imaging of Pharmacokinetics and Biodistribution in Multiple Organs,” PLoS ONE 7(1), e30491 (2012).
[Crossref] [PubMed]

2011 (2)

A. Sarantopoulos, G. Themelis, and V. Ntziachristos, “Imaging the bio-distribution of fluorescent probes using multispectral epi-illumination cryoslicing imaging,” Mol. Imaging Biol. 13(5), 874–885 (2011).
[Crossref] [PubMed]

P. Beard, “Biomedical photoacoustic imaging,” Interface Focus 1(4), 602–631 (2011).
[Crossref] [PubMed]

2010 (5)

M. V. Marshall, D. Draney, E. M. Sevick-Muraca, and D. M. Olive, “Single-dose intravenous toxicity study of IRDye 800CW in Sprague-Dawley rats,” Mol. Imaging Biol. 12(6), 583–594 (2010).
[Crossref] [PubMed]

V. Ntziachristos, “Going deeper than microscopy: the optical imaging frontier in biology,” Nat. Methods 7(8), 603–614 (2010).
[Crossref] [PubMed]

B. Ergin, S. Meding, R. Langer, M. Kap, C. Viertler, C. Schott, U. Ferch, P. Riegman, K. Zatloukal, A. Walch, and K. F. Becker, “Proteomic analysis of PAXgene-fixed tissues,” J. Proteome Res. 9(10), 5188–5196 (2010).
[Crossref] [PubMed]

A. Taruttis, E. Herzog, D. Razansky, and V. Ntziachristos, “Real-time imaging of cardiovascular dynamics and circulating gold nanorods with multispectral optoacoustic tomography,” Opt. Express 18(19), 19592–19602 (2010).
[PubMed]

T. Jetzfellner, A. Rosenthal, A. Buehler, A. Dima, K.-H. Englmeier, V. Ntziachristos, and D. Razansky, “Optoacoustic tomography with varying illumination and non-uniform detection patterns,” J. Opt. Soc. Am. A 27(11), 2488–2495 (2010).
[Crossref] [PubMed]

2009 (3)

2008 (2)

T. Lasser, A. Soubret, J. Ripoll, and V. Ntziachristos, “Surface reconstruction for free-space 360 degrees fluorescence molecular tomography and the effects of animal motion,” IEEE Trans. Med. Imaging 27(2), 188–194 (2008).
[Crossref] [PubMed]

E. Zhang, J. Laufer, and P. Beard, “Backward-mode multiwavelength photoacoustic scanner using a planar Fabry-Perot polymer film ultrasound sensor for high-resolution three-dimensional imaging of biological tissues,” Appl. Opt. 47(4), 561–577 (2008).
[Crossref] [PubMed]

2007 (3)

T. Alanentalo, A. Asayesh, H. Morrison, C. E. Lorén, D. Holmberg, J. Sharpe, and U. Ahlgren, “Tomographic molecular imaging and 3D quantification within adult mouse organs,” Nat. Methods 4(1), 31–33 (2007).
[Crossref] [PubMed]

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2007).
[Crossref] [PubMed]

H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[Crossref] [PubMed]

2006 (2)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, “Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging,” Nat. Biotechnol. 24(7), 848–851 (2006).
[Crossref] [PubMed]

Y. Liu, “Renal fibrosis: new insights into the pathogenesis and therapeutics,” Kidney Int. 69(2), 213–217 (2006).
[Crossref] [PubMed]

2004 (2)

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

G. Ku, X. Wang, G. Stoica, and L. V. Wang, “Multiple-bandwidth photoacoustic tomography,” Phys. Med. Biol. 49(7), 1329–1338 (2004).
[Crossref] [PubMed]

2002 (1)

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002).
[Crossref] [PubMed]

2000 (1)

R. Mañalich, L. Reyes, M. Herrera, C. Melendi, and I. Fundora, “Relationship between weight at birth and the number and size of renal glomeruli in humans: a histomorphometric study,” Kidney Int. 58(2), 770–773 (2000).
[Crossref] [PubMed]

1990 (1)

F. J. Sampaio and A. H. Aragao, “Anatomical relationship between the intrarenal arteries and the kidney collecting system,” J. Urol. 143(4), 679–681 (1990).
[PubMed]

1974 (1)

W. Kriz and H. Koepsell, “The structural organization of the mouse kidney,” Z. Anat. Entwicklungsgesch. 144(2), 137–163 (1974).
[Crossref] [PubMed]

Ahlgren, U.

T. Alanentalo, A. Asayesh, H. Morrison, C. E. Lorén, D. Holmberg, J. Sharpe, and U. Ahlgren, “Tomographic molecular imaging and 3D quantification within adult mouse organs,” Nat. Methods 4(1), 31–33 (2007).
[Crossref] [PubMed]

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002).
[Crossref] [PubMed]

Alanentalo, T.

T. Alanentalo, A. Asayesh, H. Morrison, C. E. Lorén, D. Holmberg, J. Sharpe, and U. Ahlgren, “Tomographic molecular imaging and 3D quantification within adult mouse organs,” Nat. Methods 4(1), 31–33 (2007).
[Crossref] [PubMed]

Aragao, A. H.

F. J. Sampaio and A. H. Aragao, “Anatomical relationship between the intrarenal arteries and the kidney collecting system,” J. Urol. 143(4), 679–681 (1990).
[PubMed]

Araque Caballero, M. A.

M. A. Araque Caballero, J. Gateau, X.-L. Dean-Ben, and V. Ntziachristos, “Model-based optoacoustic image reconstruction of large three-dimensional tomographic datasets acquired with an array of directional detectors,” IEEE Trans. Med. Imaging 33(2), 433–443 (2014).
[Crossref] [PubMed]

Asayesh, A.

T. Alanentalo, A. Asayesh, H. Morrison, C. E. Lorén, D. Holmberg, J. Sharpe, and U. Ahlgren, “Tomographic molecular imaging and 3D quantification within adult mouse organs,” Nat. Methods 4(1), 31–33 (2007).
[Crossref] [PubMed]

Baldock, R.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002).
[Crossref] [PubMed]

Beard, P.

Becker, K.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[Crossref] [PubMed]

Becker, K. F.

B. Ergin, S. Meding, R. Langer, M. Kap, C. Viertler, C. Schott, U. Ferch, P. Riegman, K. Zatloukal, A. Walch, and K. F. Becker, “Proteomic analysis of PAXgene-fixed tissues,” J. Proteome Res. 9(10), 5188–5196 (2010).
[Crossref] [PubMed]

Beziere, N.

E. Herzog, A. Taruttis, N. Beziere, A. A. Lutich, D. Razansky, and V. Ntziachristos, “Optical Imaging of Cancer Heterogeneity with Multispectral Optoacoustic Tomography,” Radiology 263(2), 461–468 (2012).
[Crossref] [PubMed]

Bozhko, D.

A. Rosenthal, S. Kellnberger, D. Bozhko, A. Chekkoury, M. Omar, D. Razansky, and V. Ntziachristos, “Sensitive interferometric detection of ultrasound for minimally invasive clinical imaging applications,” Laser Photon. Rev. 8(3), 450–457 (2014).
[Crossref]

Buehler, A.

Burton, N. C.

A. Taruttis, S. Morscher, N. C. Burton, D. Razansky, and V. Ntziachristos, “Fast Multispectral Optoacoustic Tomography (MSOT) for Dynamic Imaging of Pharmacokinetics and Biodistribution in Multiple Organs,” PLoS ONE 7(1), e30491 (2012).
[Crossref] [PubMed]

Caballero, M. A. A.

J. Gateau, M. A. A. Caballero, A. Dima, and V. Ntziachristos, “Three-dimensional optoacoustic tomography using a conventional ultrasound linear detector array: Whole-body tomographic system for small animals,” Med. Phys. 40(1), 013302 (2013).
[Crossref] [PubMed]

Chekkoury, A.

A. Rosenthal, S. Kellnberger, D. Bozhko, A. Chekkoury, M. Omar, D. Razansky, and V. Ntziachristos, “Sensitive interferometric detection of ultrasound for minimally invasive clinical imaging applications,” Laser Photon. Rev. 8(3), 450–457 (2014).
[Crossref]

J. Gateau, A. Chekkoury, and V. Ntziachristos, “High-resolution optoacoustic mesoscopy with a 24 MHz multidetector translate-rotate scanner,” J. Biomed. Opt. 18(10), 106005 (2013).
[Crossref] [PubMed]

J. Gateau, A. Chekkoury, and V. Ntziachristos, “Ultra-wideband three-dimensional optoacoustic tomography,” Opt. Lett. 38(22), 4671–4674 (2013).
[Crossref] [PubMed]

Davidson, D.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002).
[Crossref] [PubMed]

Dean-Ben, X.-L.

M. A. Araque Caballero, J. Gateau, X.-L. Dean-Ben, and V. Ntziachristos, “Model-based optoacoustic image reconstruction of large three-dimensional tomographic datasets acquired with an array of directional detectors,” IEEE Trans. Med. Imaging 33(2), 433–443 (2014).
[Crossref] [PubMed]

Deininger, K.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[Crossref] [PubMed]

Del Bene, F.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Deliolanis, N.

S. Tzoumas, N. Deliolanis, S. Morscher, and V. Ntziachristos, “Unmixing Molecular Agents From Absorbing Tissue in Multispectral Optoacoustic Tomography,” IEEE Trans. Med. Imaging 33(1), 48–60 (2014).
[Crossref]

Deussing, J. M.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[Crossref] [PubMed]

Dima, A.

J. Gateau, M. A. A. Caballero, A. Dima, and V. Ntziachristos, “Three-dimensional optoacoustic tomography using a conventional ultrasound linear detector array: Whole-body tomographic system for small animals,” Med. Phys. 40(1), 013302 (2013).
[Crossref] [PubMed]

T. Jetzfellner, A. Rosenthal, A. Buehler, A. Dima, K.-H. Englmeier, V. Ntziachristos, and D. Razansky, “Optoacoustic tomography with varying illumination and non-uniform detection patterns,” J. Opt. Soc. Am. A 27(11), 2488–2495 (2010).
[Crossref] [PubMed]

Distel, M.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, “Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo,” Nat. Photonics 3(7), 412–417 (2009).
[Crossref]

Dobosz, M.

M. Dobosz, V. Ntziachristos, W. Scheuer, and S. Strobel, “Multispectral Fluorescence Ultramicroscopy: Three-Dimensional Visualization and Automatic Quantification of Tumor Morphology, Drug Penetration, and Antiangiogenic Treatment Response,” Neoplasia 16(1), 1–13 (2014).
[Crossref] [PubMed]

Dodt, H. U.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[Crossref] [PubMed]

Draney, D.

M. V. Marshall, D. Draney, E. M. Sevick-Muraca, and D. M. Olive, “Single-dose intravenous toxicity study of IRDye 800CW in Sprague-Dawley rats,” Mol. Imaging Biol. 12(6), 583–594 (2010).
[Crossref] [PubMed]

Dufait, L.

S. Michau, P. Mauchamp, and L. Dufait, “Piezocomposite 30MHz linear array for medical imaging: design challenges and performances evaluation of a 128 elements array,” in Ultrasonics Symposium, 2004 IEEE, (IEEE, 2004), 898–901.
[Crossref]

Eder, M.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[Crossref] [PubMed]

Englmeier, K.-H.

Ergin, B.

B. Ergin, S. Meding, R. Langer, M. Kap, C. Viertler, C. Schott, U. Ferch, P. Riegman, K. Zatloukal, A. Walch, and K. F. Becker, “Proteomic analysis of PAXgene-fixed tissues,” J. Proteome Res. 9(10), 5188–5196 (2010).
[Crossref] [PubMed]

Ferch, U.

B. Ergin, S. Meding, R. Langer, M. Kap, C. Viertler, C. Schott, U. Ferch, P. Riegman, K. Zatloukal, A. Walch, and K. F. Becker, “Proteomic analysis of PAXgene-fixed tissues,” J. Proteome Res. 9(10), 5188–5196 (2010).
[Crossref] [PubMed]

Fundora, I.

R. Mañalich, L. Reyes, M. Herrera, C. Melendi, and I. Fundora, “Relationship between weight at birth and the number and size of renal glomeruli in humans: a histomorphometric study,” Kidney Int. 58(2), 770–773 (2000).
[Crossref] [PubMed]

Gateau, J.

M. A. Araque Caballero, J. Gateau, X.-L. Dean-Ben, and V. Ntziachristos, “Model-based optoacoustic image reconstruction of large three-dimensional tomographic datasets acquired with an array of directional detectors,” IEEE Trans. Med. Imaging 33(2), 433–443 (2014).
[Crossref] [PubMed]

J. Gateau, M. A. A. Caballero, A. Dima, and V. Ntziachristos, “Three-dimensional optoacoustic tomography using a conventional ultrasound linear detector array: Whole-body tomographic system for small animals,” Med. Phys. 40(1), 013302 (2013).
[Crossref] [PubMed]

J. Gateau, A. Chekkoury, and V. Ntziachristos, “High-resolution optoacoustic mesoscopy with a 24 MHz multidetector translate-rotate scanner,” J. Biomed. Opt. 18(10), 106005 (2013).
[Crossref] [PubMed]

J. Gateau, A. Chekkoury, and V. Ntziachristos, “Ultra-wideband three-dimensional optoacoustic tomography,” Opt. Lett. 38(22), 4671–4674 (2013).
[Crossref] [PubMed]

M. Omar, J. Gateau, and V. Ntziachristos, “Raster-scan optoacoustic mesoscopy in the 25-125 MHz range.”

Hecksher-Sørensen, J.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002).
[Crossref] [PubMed]

Herrera, M.

R. Mañalich, L. Reyes, M. Herrera, C. Melendi, and I. Fundora, “Relationship between weight at birth and the number and size of renal glomeruli in humans: a histomorphometric study,” Kidney Int. 58(2), 770–773 (2000).
[Crossref] [PubMed]

Herzog, E.

E. Herzog, A. Taruttis, N. Beziere, A. A. Lutich, D. Razansky, and V. Ntziachristos, “Optical Imaging of Cancer Heterogeneity with Multispectral Optoacoustic Tomography,” Radiology 263(2), 461–468 (2012).
[Crossref] [PubMed]

A. Taruttis, E. Herzog, D. Razansky, and V. Ntziachristos, “Real-time imaging of cardiovascular dynamics and circulating gold nanorods with multispectral optoacoustic tomography,” Opt. Express 18(19), 19592–19602 (2010).
[PubMed]

Hill, B.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002).
[Crossref] [PubMed]

Holmberg, D.

T. Alanentalo, A. Asayesh, H. Morrison, C. E. Lorén, D. Holmberg, J. Sharpe, and U. Ahlgren, “Tomographic molecular imaging and 3D quantification within adult mouse organs,” Nat. Methods 4(1), 31–33 (2007).
[Crossref] [PubMed]

Hu, S.

L. V. Wang and S. Hu, “Photoacoustic tomography: in vivo imaging from organelles to organs,” Science 335(6075), 1458–1462 (2012).
[Crossref] [PubMed]

Huisken, J.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Jährling, N.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[Crossref] [PubMed]

Jetzfellner, T.

Kap, M.

B. Ergin, S. Meding, R. Langer, M. Kap, C. Viertler, C. Schott, U. Ferch, P. Riegman, K. Zatloukal, A. Walch, and K. F. Becker, “Proteomic analysis of PAXgene-fixed tissues,” J. Proteome Res. 9(10), 5188–5196 (2010).
[Crossref] [PubMed]

Kellnberger, S.

A. Rosenthal, S. Kellnberger, D. Bozhko, A. Chekkoury, M. Omar, D. Razansky, and V. Ntziachristos, “Sensitive interferometric detection of ultrasound for minimally invasive clinical imaging applications,” Laser Photon. Rev. 8(3), 450–457 (2014).
[Crossref]

Koepsell, H.

W. Kriz and H. Koepsell, “The structural organization of the mouse kidney,” Z. Anat. Entwicklungsgesch. 144(2), 137–163 (1974).
[Crossref] [PubMed]

Köster, R. W.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, “Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo,” Nat. Photonics 3(7), 412–417 (2009).
[Crossref]

Kriz, W.

W. Kriz and H. Koepsell, “The structural organization of the mouse kidney,” Z. Anat. Entwicklungsgesch. 144(2), 137–163 (1974).
[Crossref] [PubMed]

Ku, G.

G. Ku, X. Wang, G. Stoica, and L. V. Wang, “Multiple-bandwidth photoacoustic tomography,” Phys. Med. Biol. 49(7), 1329–1338 (2004).
[Crossref] [PubMed]

Langer, R.

B. Ergin, S. Meding, R. Langer, M. Kap, C. Viertler, C. Schott, U. Ferch, P. Riegman, K. Zatloukal, A. Walch, and K. F. Becker, “Proteomic analysis of PAXgene-fixed tissues,” J. Proteome Res. 9(10), 5188–5196 (2010).
[Crossref] [PubMed]

Lasser, T.

T. Lasser, A. Soubret, J. Ripoll, and V. Ntziachristos, “Surface reconstruction for free-space 360 degrees fluorescence molecular tomography and the effects of animal motion,” IEEE Trans. Med. Imaging 27(2), 188–194 (2008).
[Crossref] [PubMed]

Laufer, J.

Leischner, U.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[Crossref] [PubMed]

Liu, Y.

Y. Liu, “Renal fibrosis: new insights into the pathogenesis and therapeutics,” Kidney Int. 69(2), 213–217 (2006).
[Crossref] [PubMed]

Lorén, C. E.

T. Alanentalo, A. Asayesh, H. Morrison, C. E. Lorén, D. Holmberg, J. Sharpe, and U. Ahlgren, “Tomographic molecular imaging and 3D quantification within adult mouse organs,” Nat. Methods 4(1), 31–33 (2007).
[Crossref] [PubMed]

Lutich, A. A.

E. Herzog, A. Taruttis, N. Beziere, A. A. Lutich, D. Razansky, and V. Ntziachristos, “Optical Imaging of Cancer Heterogeneity with Multispectral Optoacoustic Tomography,” Radiology 263(2), 461–468 (2012).
[Crossref] [PubMed]

Ma, R.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, “Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo,” Nat. Photonics 3(7), 412–417 (2009).
[Crossref]

R. Ma, A. Taruttis, V. Ntziachristos, and D. Razansky, “Multispectral optoacoustic tomography (MSOT) scanner for whole-body small animal imaging,” Opt. Express 17(24), 21414–21426 (2009).
[Crossref] [PubMed]

Mañalich, R.

R. Mañalich, L. Reyes, M. Herrera, C. Melendi, and I. Fundora, “Relationship between weight at birth and the number and size of renal glomeruli in humans: a histomorphometric study,” Kidney Int. 58(2), 770–773 (2000).
[Crossref] [PubMed]

Marshall, M. V.

M. V. Marshall, D. Draney, E. M. Sevick-Muraca, and D. M. Olive, “Single-dose intravenous toxicity study of IRDye 800CW in Sprague-Dawley rats,” Mol. Imaging Biol. 12(6), 583–594 (2010).
[Crossref] [PubMed]

Maslov, K.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, “Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging,” Nat. Biotechnol. 24(7), 848–851 (2006).
[Crossref] [PubMed]

Mauch, C. P.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[Crossref] [PubMed]

Mauchamp, P.

S. Michau, P. Mauchamp, and L. Dufait, “Piezocomposite 30MHz linear array for medical imaging: design challenges and performances evaluation of a 128 elements array,” in Ultrasonics Symposium, 2004 IEEE, (IEEE, 2004), 898–901.
[Crossref]

Meding, S.

B. Ergin, S. Meding, R. Langer, M. Kap, C. Viertler, C. Schott, U. Ferch, P. Riegman, K. Zatloukal, A. Walch, and K. F. Becker, “Proteomic analysis of PAXgene-fixed tissues,” J. Proteome Res. 9(10), 5188–5196 (2010).
[Crossref] [PubMed]

Melendi, C.

R. Mañalich, L. Reyes, M. Herrera, C. Melendi, and I. Fundora, “Relationship between weight at birth and the number and size of renal glomeruli in humans: a histomorphometric study,” Kidney Int. 58(2), 770–773 (2000).
[Crossref] [PubMed]

Michau, S.

S. Michau, P. Mauchamp, and L. Dufait, “Piezocomposite 30MHz linear array for medical imaging: design challenges and performances evaluation of a 128 elements array,” in Ultrasonics Symposium, 2004 IEEE, (IEEE, 2004), 898–901.
[Crossref]

Morrison, H.

T. Alanentalo, A. Asayesh, H. Morrison, C. E. Lorén, D. Holmberg, J. Sharpe, and U. Ahlgren, “Tomographic molecular imaging and 3D quantification within adult mouse organs,” Nat. Methods 4(1), 31–33 (2007).
[Crossref] [PubMed]

Morscher, S.

S. Tzoumas, N. Deliolanis, S. Morscher, and V. Ntziachristos, “Unmixing Molecular Agents From Absorbing Tissue in Multispectral Optoacoustic Tomography,” IEEE Trans. Med. Imaging 33(1), 48–60 (2014).
[Crossref]

A. Taruttis, S. Morscher, N. C. Burton, D. Razansky, and V. Ntziachristos, “Fast Multispectral Optoacoustic Tomography (MSOT) for Dynamic Imaging of Pharmacokinetics and Biodistribution in Multiple Organs,” PLoS ONE 7(1), e30491 (2012).
[Crossref] [PubMed]

Ntziachristos, V.

M. Dobosz, V. Ntziachristos, W. Scheuer, and S. Strobel, “Multispectral Fluorescence Ultramicroscopy: Three-Dimensional Visualization and Automatic Quantification of Tumor Morphology, Drug Penetration, and Antiangiogenic Treatment Response,” Neoplasia 16(1), 1–13 (2014).
[Crossref] [PubMed]

S. Tzoumas, N. Deliolanis, S. Morscher, and V. Ntziachristos, “Unmixing Molecular Agents From Absorbing Tissue in Multispectral Optoacoustic Tomography,” IEEE Trans. Med. Imaging 33(1), 48–60 (2014).
[Crossref]

M. A. Araque Caballero, J. Gateau, X.-L. Dean-Ben, and V. Ntziachristos, “Model-based optoacoustic image reconstruction of large three-dimensional tomographic datasets acquired with an array of directional detectors,” IEEE Trans. Med. Imaging 33(2), 433–443 (2014).
[Crossref] [PubMed]

A. Rosenthal, S. Kellnberger, D. Bozhko, A. Chekkoury, M. Omar, D. Razansky, and V. Ntziachristos, “Sensitive interferometric detection of ultrasound for minimally invasive clinical imaging applications,” Laser Photon. Rev. 8(3), 450–457 (2014).
[Crossref]

J. Gateau, M. A. A. Caballero, A. Dima, and V. Ntziachristos, “Three-dimensional optoacoustic tomography using a conventional ultrasound linear detector array: Whole-body tomographic system for small animals,” Med. Phys. 40(1), 013302 (2013).
[Crossref] [PubMed]

J. Gateau, A. Chekkoury, and V. Ntziachristos, “High-resolution optoacoustic mesoscopy with a 24 MHz multidetector translate-rotate scanner,” J. Biomed. Opt. 18(10), 106005 (2013).
[Crossref] [PubMed]

J. Gateau, A. Chekkoury, and V. Ntziachristos, “Ultra-wideband three-dimensional optoacoustic tomography,” Opt. Lett. 38(22), 4671–4674 (2013).
[Crossref] [PubMed]

E. Herzog, A. Taruttis, N. Beziere, A. A. Lutich, D. Razansky, and V. Ntziachristos, “Optical Imaging of Cancer Heterogeneity with Multispectral Optoacoustic Tomography,” Radiology 263(2), 461–468 (2012).
[Crossref] [PubMed]

A. Taruttis, S. Morscher, N. C. Burton, D. Razansky, and V. Ntziachristos, “Fast Multispectral Optoacoustic Tomography (MSOT) for Dynamic Imaging of Pharmacokinetics and Biodistribution in Multiple Organs,” PLoS ONE 7(1), e30491 (2012).
[Crossref] [PubMed]

A. Sarantopoulos, G. Themelis, and V. Ntziachristos, “Imaging the bio-distribution of fluorescent probes using multispectral epi-illumination cryoslicing imaging,” Mol. Imaging Biol. 13(5), 874–885 (2011).
[Crossref] [PubMed]

V. Ntziachristos, “Going deeper than microscopy: the optical imaging frontier in biology,” Nat. Methods 7(8), 603–614 (2010).
[Crossref] [PubMed]

A. Taruttis, E. Herzog, D. Razansky, and V. Ntziachristos, “Real-time imaging of cardiovascular dynamics and circulating gold nanorods with multispectral optoacoustic tomography,” Opt. Express 18(19), 19592–19602 (2010).
[PubMed]

T. Jetzfellner, A. Rosenthal, A. Buehler, A. Dima, K.-H. Englmeier, V. Ntziachristos, and D. Razansky, “Optoacoustic tomography with varying illumination and non-uniform detection patterns,” J. Opt. Soc. Am. A 27(11), 2488–2495 (2010).
[Crossref] [PubMed]

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, “Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo,” Nat. Photonics 3(7), 412–417 (2009).
[Crossref]

R. Ma, A. Taruttis, V. Ntziachristos, and D. Razansky, “Multispectral optoacoustic tomography (MSOT) scanner for whole-body small animal imaging,” Opt. Express 17(24), 21414–21426 (2009).
[Crossref] [PubMed]

T. Lasser, A. Soubret, J. Ripoll, and V. Ntziachristos, “Surface reconstruction for free-space 360 degrees fluorescence molecular tomography and the effects of animal motion,” IEEE Trans. Med. Imaging 27(2), 188–194 (2008).
[Crossref] [PubMed]

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2007).
[Crossref] [PubMed]

M. Omar, J. Gateau, and V. Ntziachristos, “Raster-scan optoacoustic mesoscopy in the 25-125 MHz range.”

Olive, D. M.

M. V. Marshall, D. Draney, E. M. Sevick-Muraca, and D. M. Olive, “Single-dose intravenous toxicity study of IRDye 800CW in Sprague-Dawley rats,” Mol. Imaging Biol. 12(6), 583–594 (2010).
[Crossref] [PubMed]

Omar, M.

A. Rosenthal, S. Kellnberger, D. Bozhko, A. Chekkoury, M. Omar, D. Razansky, and V. Ntziachristos, “Sensitive interferometric detection of ultrasound for minimally invasive clinical imaging applications,” Laser Photon. Rev. 8(3), 450–457 (2014).
[Crossref]

M. Omar, J. Gateau, and V. Ntziachristos, “Raster-scan optoacoustic mesoscopy in the 25-125 MHz range.”

Perrimon, N.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, “Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo,” Nat. Photonics 3(7), 412–417 (2009).
[Crossref]

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2007).
[Crossref] [PubMed]

Perry, P.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002).
[Crossref] [PubMed]

Pitsouli, C.

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2007).
[Crossref] [PubMed]

Raivich, G.

Razansky, D.

A. Rosenthal, S. Kellnberger, D. Bozhko, A. Chekkoury, M. Omar, D. Razansky, and V. Ntziachristos, “Sensitive interferometric detection of ultrasound for minimally invasive clinical imaging applications,” Laser Photon. Rev. 8(3), 450–457 (2014).
[Crossref]

A. Taruttis, S. Morscher, N. C. Burton, D. Razansky, and V. Ntziachristos, “Fast Multispectral Optoacoustic Tomography (MSOT) for Dynamic Imaging of Pharmacokinetics and Biodistribution in Multiple Organs,” PLoS ONE 7(1), e30491 (2012).
[Crossref] [PubMed]

E. Herzog, A. Taruttis, N. Beziere, A. A. Lutich, D. Razansky, and V. Ntziachristos, “Optical Imaging of Cancer Heterogeneity with Multispectral Optoacoustic Tomography,” Radiology 263(2), 461–468 (2012).
[Crossref] [PubMed]

A. Taruttis, E. Herzog, D. Razansky, and V. Ntziachristos, “Real-time imaging of cardiovascular dynamics and circulating gold nanorods with multispectral optoacoustic tomography,” Opt. Express 18(19), 19592–19602 (2010).
[PubMed]

T. Jetzfellner, A. Rosenthal, A. Buehler, A. Dima, K.-H. Englmeier, V. Ntziachristos, and D. Razansky, “Optoacoustic tomography with varying illumination and non-uniform detection patterns,” J. Opt. Soc. Am. A 27(11), 2488–2495 (2010).
[Crossref] [PubMed]

R. Ma, A. Taruttis, V. Ntziachristos, and D. Razansky, “Multispectral optoacoustic tomography (MSOT) scanner for whole-body small animal imaging,” Opt. Express 17(24), 21414–21426 (2009).
[Crossref] [PubMed]

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, “Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo,” Nat. Photonics 3(7), 412–417 (2009).
[Crossref]

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2007).
[Crossref] [PubMed]

Reyes, L.

R. Mañalich, L. Reyes, M. Herrera, C. Melendi, and I. Fundora, “Relationship between weight at birth and the number and size of renal glomeruli in humans: a histomorphometric study,” Kidney Int. 58(2), 770–773 (2000).
[Crossref] [PubMed]

Riegman, P.

B. Ergin, S. Meding, R. Langer, M. Kap, C. Viertler, C. Schott, U. Ferch, P. Riegman, K. Zatloukal, A. Walch, and K. F. Becker, “Proteomic analysis of PAXgene-fixed tissues,” J. Proteome Res. 9(10), 5188–5196 (2010).
[Crossref] [PubMed]

Ripoll, J.

T. Lasser, A. Soubret, J. Ripoll, and V. Ntziachristos, “Surface reconstruction for free-space 360 degrees fluorescence molecular tomography and the effects of animal motion,” IEEE Trans. Med. Imaging 27(2), 188–194 (2008).
[Crossref] [PubMed]

Rosenthal, A.

A. Rosenthal, S. Kellnberger, D. Bozhko, A. Chekkoury, M. Omar, D. Razansky, and V. Ntziachristos, “Sensitive interferometric detection of ultrasound for minimally invasive clinical imaging applications,” Laser Photon. Rev. 8(3), 450–457 (2014).
[Crossref]

T. Jetzfellner, A. Rosenthal, A. Buehler, A. Dima, K.-H. Englmeier, V. Ntziachristos, and D. Razansky, “Optoacoustic tomography with varying illumination and non-uniform detection patterns,” J. Opt. Soc. Am. A 27(11), 2488–2495 (2010).
[Crossref] [PubMed]

Ross, A.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002).
[Crossref] [PubMed]

Sampaio, F. J.

F. J. Sampaio and A. H. Aragao, “Anatomical relationship between the intrarenal arteries and the kidney collecting system,” J. Urol. 143(4), 679–681 (1990).
[PubMed]

Sarantopoulos, A.

A. Sarantopoulos, G. Themelis, and V. Ntziachristos, “Imaging the bio-distribution of fluorescent probes using multispectral epi-illumination cryoslicing imaging,” Mol. Imaging Biol. 13(5), 874–885 (2011).
[Crossref] [PubMed]

Scheuer, W.

M. Dobosz, V. Ntziachristos, W. Scheuer, and S. Strobel, “Multispectral Fluorescence Ultramicroscopy: Three-Dimensional Visualization and Automatic Quantification of Tumor Morphology, Drug Penetration, and Antiangiogenic Treatment Response,” Neoplasia 16(1), 1–13 (2014).
[Crossref] [PubMed]

Schierloh, A.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[Crossref] [PubMed]

Schott, C.

B. Ergin, S. Meding, R. Langer, M. Kap, C. Viertler, C. Schott, U. Ferch, P. Riegman, K. Zatloukal, A. Walch, and K. F. Becker, “Proteomic analysis of PAXgene-fixed tissues,” J. Proteome Res. 9(10), 5188–5196 (2010).
[Crossref] [PubMed]

Sevick-Muraca, E. M.

M. V. Marshall, D. Draney, E. M. Sevick-Muraca, and D. M. Olive, “Single-dose intravenous toxicity study of IRDye 800CW in Sprague-Dawley rats,” Mol. Imaging Biol. 12(6), 583–594 (2010).
[Crossref] [PubMed]

Sharpe, J.

T. Alanentalo, A. Asayesh, H. Morrison, C. E. Lorén, D. Holmberg, J. Sharpe, and U. Ahlgren, “Tomographic molecular imaging and 3D quantification within adult mouse organs,” Nat. Methods 4(1), 31–33 (2007).
[Crossref] [PubMed]

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002).
[Crossref] [PubMed]

Soubret, A.

T. Lasser, A. Soubret, J. Ripoll, and V. Ntziachristos, “Surface reconstruction for free-space 360 degrees fluorescence molecular tomography and the effects of animal motion,” IEEE Trans. Med. Imaging 27(2), 188–194 (2008).
[Crossref] [PubMed]

Stelzer, E. H.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Stoica, G.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, “Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging,” Nat. Biotechnol. 24(7), 848–851 (2006).
[Crossref] [PubMed]

G. Ku, X. Wang, G. Stoica, and L. V. Wang, “Multiple-bandwidth photoacoustic tomography,” Phys. Med. Biol. 49(7), 1329–1338 (2004).
[Crossref] [PubMed]

Strobel, S.

M. Dobosz, V. Ntziachristos, W. Scheuer, and S. Strobel, “Multispectral Fluorescence Ultramicroscopy: Three-Dimensional Visualization and Automatic Quantification of Tumor Morphology, Drug Penetration, and Antiangiogenic Treatment Response,” Neoplasia 16(1), 1–13 (2014).
[Crossref] [PubMed]

Swoger, J.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Taruttis, A.

E. Herzog, A. Taruttis, N. Beziere, A. A. Lutich, D. Razansky, and V. Ntziachristos, “Optical Imaging of Cancer Heterogeneity with Multispectral Optoacoustic Tomography,” Radiology 263(2), 461–468 (2012).
[Crossref] [PubMed]

A. Taruttis, S. Morscher, N. C. Burton, D. Razansky, and V. Ntziachristos, “Fast Multispectral Optoacoustic Tomography (MSOT) for Dynamic Imaging of Pharmacokinetics and Biodistribution in Multiple Organs,” PLoS ONE 7(1), e30491 (2012).
[Crossref] [PubMed]

A. Taruttis, E. Herzog, D. Razansky, and V. Ntziachristos, “Real-time imaging of cardiovascular dynamics and circulating gold nanorods with multispectral optoacoustic tomography,” Opt. Express 18(19), 19592–19602 (2010).
[PubMed]

R. Ma, A. Taruttis, V. Ntziachristos, and D. Razansky, “Multispectral optoacoustic tomography (MSOT) scanner for whole-body small animal imaging,” Opt. Express 17(24), 21414–21426 (2009).
[Crossref] [PubMed]

Themelis, G.

A. Sarantopoulos, G. Themelis, and V. Ntziachristos, “Imaging the bio-distribution of fluorescent probes using multispectral epi-illumination cryoslicing imaging,” Mol. Imaging Biol. 13(5), 874–885 (2011).
[Crossref] [PubMed]

Tzoumas, S.

S. Tzoumas, N. Deliolanis, S. Morscher, and V. Ntziachristos, “Unmixing Molecular Agents From Absorbing Tissue in Multispectral Optoacoustic Tomography,” IEEE Trans. Med. Imaging 33(1), 48–60 (2014).
[Crossref]

Viertler, C.

B. Ergin, S. Meding, R. Langer, M. Kap, C. Viertler, C. Schott, U. Ferch, P. Riegman, K. Zatloukal, A. Walch, and K. F. Becker, “Proteomic analysis of PAXgene-fixed tissues,” J. Proteome Res. 9(10), 5188–5196 (2010).
[Crossref] [PubMed]

Vinegoni, C.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, “Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo,” Nat. Photonics 3(7), 412–417 (2009).
[Crossref]

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2007).
[Crossref] [PubMed]

Walch, A.

B. Ergin, S. Meding, R. Langer, M. Kap, C. Viertler, C. Schott, U. Ferch, P. Riegman, K. Zatloukal, A. Walch, and K. F. Becker, “Proteomic analysis of PAXgene-fixed tissues,” J. Proteome Res. 9(10), 5188–5196 (2010).
[Crossref] [PubMed]

Wang, L. V.

L. V. Wang and S. Hu, “Photoacoustic tomography: in vivo imaging from organelles to organs,” Science 335(6075), 1458–1462 (2012).
[Crossref] [PubMed]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, “Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging,” Nat. Biotechnol. 24(7), 848–851 (2006).
[Crossref] [PubMed]

G. Ku, X. Wang, G. Stoica, and L. V. Wang, “Multiple-bandwidth photoacoustic tomography,” Phys. Med. Biol. 49(7), 1329–1338 (2004).
[Crossref] [PubMed]

Wang, X.

G. Ku, X. Wang, G. Stoica, and L. V. Wang, “Multiple-bandwidth photoacoustic tomography,” Phys. Med. Biol. 49(7), 1329–1338 (2004).
[Crossref] [PubMed]

Wittbrodt, J.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Zatloukal, K.

B. Ergin, S. Meding, R. Langer, M. Kap, C. Viertler, C. Schott, U. Ferch, P. Riegman, K. Zatloukal, A. Walch, and K. F. Becker, “Proteomic analysis of PAXgene-fixed tissues,” J. Proteome Res. 9(10), 5188–5196 (2010).
[Crossref] [PubMed]

Zhang, E.

Zhang, H. F.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, “Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging,” Nat. Biotechnol. 24(7), 848–851 (2006).
[Crossref] [PubMed]

Zieglgänsberger, W.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[Crossref] [PubMed]

Appl. Opt. (2)

IEEE Trans. Med. Imaging (3)

S. Tzoumas, N. Deliolanis, S. Morscher, and V. Ntziachristos, “Unmixing Molecular Agents From Absorbing Tissue in Multispectral Optoacoustic Tomography,” IEEE Trans. Med. Imaging 33(1), 48–60 (2014).
[Crossref]

M. A. Araque Caballero, J. Gateau, X.-L. Dean-Ben, and V. Ntziachristos, “Model-based optoacoustic image reconstruction of large three-dimensional tomographic datasets acquired with an array of directional detectors,” IEEE Trans. Med. Imaging 33(2), 433–443 (2014).
[Crossref] [PubMed]

T. Lasser, A. Soubret, J. Ripoll, and V. Ntziachristos, “Surface reconstruction for free-space 360 degrees fluorescence molecular tomography and the effects of animal motion,” IEEE Trans. Med. Imaging 27(2), 188–194 (2008).
[Crossref] [PubMed]

Interface Focus (1)

P. Beard, “Biomedical photoacoustic imaging,” Interface Focus 1(4), 602–631 (2011).
[Crossref] [PubMed]

J. Biomed. Opt. (1)

J. Gateau, A. Chekkoury, and V. Ntziachristos, “High-resolution optoacoustic mesoscopy with a 24 MHz multidetector translate-rotate scanner,” J. Biomed. Opt. 18(10), 106005 (2013).
[Crossref] [PubMed]

J. Opt. Soc. Am. A (1)

J. Proteome Res. (1)

B. Ergin, S. Meding, R. Langer, M. Kap, C. Viertler, C. Schott, U. Ferch, P. Riegman, K. Zatloukal, A. Walch, and K. F. Becker, “Proteomic analysis of PAXgene-fixed tissues,” J. Proteome Res. 9(10), 5188–5196 (2010).
[Crossref] [PubMed]

J. Urol. (1)

F. J. Sampaio and A. H. Aragao, “Anatomical relationship between the intrarenal arteries and the kidney collecting system,” J. Urol. 143(4), 679–681 (1990).
[PubMed]

Kidney Int. (2)

Y. Liu, “Renal fibrosis: new insights into the pathogenesis and therapeutics,” Kidney Int. 69(2), 213–217 (2006).
[Crossref] [PubMed]

R. Mañalich, L. Reyes, M. Herrera, C. Melendi, and I. Fundora, “Relationship between weight at birth and the number and size of renal glomeruli in humans: a histomorphometric study,” Kidney Int. 58(2), 770–773 (2000).
[Crossref] [PubMed]

Laser Photon. Rev. (1)

A. Rosenthal, S. Kellnberger, D. Bozhko, A. Chekkoury, M. Omar, D. Razansky, and V. Ntziachristos, “Sensitive interferometric detection of ultrasound for minimally invasive clinical imaging applications,” Laser Photon. Rev. 8(3), 450–457 (2014).
[Crossref]

Med. Phys. (1)

J. Gateau, M. A. A. Caballero, A. Dima, and V. Ntziachristos, “Three-dimensional optoacoustic tomography using a conventional ultrasound linear detector array: Whole-body tomographic system for small animals,” Med. Phys. 40(1), 013302 (2013).
[Crossref] [PubMed]

Mol. Imaging Biol. (2)

M. V. Marshall, D. Draney, E. M. Sevick-Muraca, and D. M. Olive, “Single-dose intravenous toxicity study of IRDye 800CW in Sprague-Dawley rats,” Mol. Imaging Biol. 12(6), 583–594 (2010).
[Crossref] [PubMed]

A. Sarantopoulos, G. Themelis, and V. Ntziachristos, “Imaging the bio-distribution of fluorescent probes using multispectral epi-illumination cryoslicing imaging,” Mol. Imaging Biol. 13(5), 874–885 (2011).
[Crossref] [PubMed]

Nat. Biotechnol. (1)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, “Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging,” Nat. Biotechnol. 24(7), 848–851 (2006).
[Crossref] [PubMed]

Nat. Methods (4)

T. Alanentalo, A. Asayesh, H. Morrison, C. E. Lorén, D. Holmberg, J. Sharpe, and U. Ahlgren, “Tomographic molecular imaging and 3D quantification within adult mouse organs,” Nat. Methods 4(1), 31–33 (2007).
[Crossref] [PubMed]

V. Ntziachristos, “Going deeper than microscopy: the optical imaging frontier in biology,” Nat. Methods 7(8), 603–614 (2010).
[Crossref] [PubMed]

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods 5(1), 45–47 (2007).
[Crossref] [PubMed]

H. U. Dodt, U. Leischner, A. Schierloh, N. Jährling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgänsberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Methods 4(4), 331–336 (2007).
[Crossref] [PubMed]

Nat. Photonics (1)

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, “Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo,” Nat. Photonics 3(7), 412–417 (2009).
[Crossref]

Neoplasia (1)

M. Dobosz, V. Ntziachristos, W. Scheuer, and S. Strobel, “Multispectral Fluorescence Ultramicroscopy: Three-Dimensional Visualization and Automatic Quantification of Tumor Morphology, Drug Penetration, and Antiangiogenic Treatment Response,” Neoplasia 16(1), 1–13 (2014).
[Crossref] [PubMed]

Opt. Express (2)

Opt. Lett. (1)

Phys. Med. Biol. (1)

G. Ku, X. Wang, G. Stoica, and L. V. Wang, “Multiple-bandwidth photoacoustic tomography,” Phys. Med. Biol. 49(7), 1329–1338 (2004).
[Crossref] [PubMed]

PLoS ONE (1)

A. Taruttis, S. Morscher, N. C. Burton, D. Razansky, and V. Ntziachristos, “Fast Multispectral Optoacoustic Tomography (MSOT) for Dynamic Imaging of Pharmacokinetics and Biodistribution in Multiple Organs,” PLoS ONE 7(1), e30491 (2012).
[Crossref] [PubMed]

Radiology (1)

E. Herzog, A. Taruttis, N. Beziere, A. A. Lutich, D. Razansky, and V. Ntziachristos, “Optical Imaging of Cancer Heterogeneity with Multispectral Optoacoustic Tomography,” Radiology 263(2), 461–468 (2012).
[Crossref] [PubMed]

Science (3)

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

L. V. Wang and S. Hu, “Photoacoustic tomography: in vivo imaging from organelles to organs,” Science 335(6075), 1458–1462 (2012).
[Crossref] [PubMed]

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sørensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296(5567), 541–545 (2002).
[Crossref] [PubMed]

Z. Anat. Entwicklungsgesch. (1)

W. Kriz and H. Koepsell, “The structural organization of the mouse kidney,” Z. Anat. Entwicklungsgesch. 144(2), 137–163 (1974).
[Crossref] [PubMed]

Other (4)

P. Treuting and S. M. Dintzis, Comparative anatomy and histology: A mouse and human atlas (Academic Press, 2011).

S. Michau, P. Mauchamp, and L. Dufait, “Piezocomposite 30MHz linear array for medical imaging: design challenges and performances evaluation of a 128 elements array,” in Ultrasonics Symposium, 2004 IEEE, (IEEE, 2004), 898–901.
[Crossref]

M. Omar, J. Gateau, and V. Ntziachristos, “Raster-scan optoacoustic mesoscopy in the 25-125 MHz range.”

M. Feuerstein, H. Heibel, J. Gardiazabal, N. Navab, and M. Groher, “Reconstruction of 3-D histology images by simultaneous deformable registration,” in Medical Image Computing and Computer-Assisted Intervention–MICCAI 2011 (Springer, 2011), pp. 582–589.

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Figures (4)

Fig. 1
Fig. 1 Experimental set-up and absorption spectra. a, Perspective view: The linear transducer array is mounted on the translation stage, which is attached to a rotation stage. Both the sample and the optical fibers are fixed. The optical fibers are mounted on a supporting holder and the laser beam is directed towards the sample to obtain a homogeneous illumination. For acoustic coupling, the sample and ultrasonic transducer were immersed in water; b, Top view of the experimental setup; c, Absorption spectra of the gold nanorods (AuNR) formulation (orange) and the fluorescent agent (IRDye CW800CW) in green. The spectra were normalized by their maximum value. The vertical lines and markers indicate, for each contrast agent, the wavelengths used for optical excitation during the multispectral acquisition.
Fig. 2
Fig. 2 Ultra-wideband Multispectral Optoacoustic Mesoscopy of Gold Nanorod detection in murine kidney – mouse #1. Spectral un-mixed images for the gold nanorods are overlaid in yellow on a single wavelength image acquired at 765 nm (gray). (First row) Maximum amplitude projection (MAP) optoacoustic images along the z-axis respectively for a, the 24-MHz array and b, the 6-MHz array; c, MAP ultramicroscopy image of the optically cleared sample. (Second row) Optoacoustic images corresponding to the in-plane slice at z = 0.7 mm for d, the 24 MHz array and e, the 6 MHz array; f, ultramicroscopy slice corresponding to a similar plane. (Third row) Optoacoustic images corresponding to the in-plane slice at z = 1.4 mm for g, the 24 MHz array and h the 6 MHz array. i, ultramicroscopy image approximately corresponding plane. (Scale bar: 2 mm.) (Panel) (I & II) Magnified view of an optoacoustic image region at (I) high-frequency and at (II) low-frequency; Dark-field Microscopy images corresponding to (III) a blood vessel (scale bar 100 µm) and to (IV) a region in the renal cortex (scale bar 10 µm); (V) DAB and CD31 staining of an artery and a vein (scale bar 50 µm), and (VI) H&E Staining of the same region (scale bar 50 µm). The boxes on the images (a,b,d,e,g) indicate the approximate location of the features shown on the panel images. The color boxes in (c,f,i) delineate regions where CA – the calyx, VE – vessels, can be visualized. Legend: RP– Renal Pelvis; SV – Segmental Vessel; MP – Medullary Pyramid;
Fig. 3
Fig. 3 Ultra-wideband Multispectral Optoacoustic Mesoscopy of Organic Dye in murine kidney - mouse #2. The optoacoustic images correspond to the peak absorption wavelength of the injected dye (774nm), and are displayed in a gray scale colormap. a, IRDye 800CW detection (green) overlay on a high-frequency 2D plane; the agent detection was based on the scan performed using the low-frequency transducer. b, Corresponding cryosection photographs and fluorescence signal detection of the agent (green); c, High-frequency, d, low frequency and e, Cryosection MAP image along y-axis; f, 2D low-frequency plane corresponding to the plane presented in a; AV – Arcuate vein; MP – Medullary Pyramid; BRA – Branching of Renal Artery; RA – Renal Artery.
Fig. 4
Fig. 4 Compartmental study of agent distribution for different frequency bands. Mean signal amplitude for 4 regions of interest selected in a, high frequency and b, low-frequency data sets; c, and d, Regions of interest selected for the high frequency analysis; e, and f, Regions selected for the low frequency analysis. Seed points for region selection overlaid on the color images: red – Renal Pelvis; yellow – renal cortex; blue – macro-vasculature; green – micro-vasculature.

Tables (1)

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Table 1 Summary of scanning parameters

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