Abstract

Here, Raman microspectroscopy was employed to assess replicative senescence of mesenchymal stem cells (MSC). A regular spectral change related to the cell senescence was found in the ratio of two peaks at 1157 cm−1 and 1174 cm−1, which are assigned to C-C, C-N stretching vibrations in proteins and C-H bending vibrations in tyrosine and phenylalanine, respectively. With the cell aging, the ratio I1157 / I1174 exhibited a monotonic decline and showed small standard deviations, so that it can statistically distinguish between cells having slight changes in terms of aging. We propose that I1157 / I1174 can act as a characteristic spectral signature for label-free assessment of MSC senescence.

© 2015 Optical Society of America

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References

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    [Crossref] [PubMed]
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    [Crossref] [PubMed]
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    [Crossref] [PubMed]
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    [Crossref]
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    [Crossref] [PubMed]
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    [Crossref] [PubMed]
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    [Crossref] [PubMed]
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    [Crossref]
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    [Crossref]
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    [Crossref] [PubMed]
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    [Crossref] [PubMed]

2014 (2)

M. C. Mitterberger, S. Lechner, M. Mattesich, and W. Zwerschke, “Adipogenic differentiation is impaired in replicative senescent human subcutaneous adipose-derived stromal/progenitor cells,” J. Gerontol. A Biol. Sci. Med. Sci. 69(1), 13–24 (2014).
[Crossref] [PubMed]

J. C. Sepúlveda, M. Tomé, M. E. Fernández, M. Delgado, J. Campisi, A. Bernad, and M. A. González, “Cell senescence abrogates the therapeutic potential of human mesenchymal stem cells in the lethal endotoxemia model,” Stem Cells 32(7), 1865–1877 (2014).
[Crossref] [PubMed]

2013 (5)

H. Hao, G. Chen, J. Liu, D. Ti, Y. Zhao, S. Xu, X. Fu, and W. Han, “Culturing on Wharton’s jelly extract delays mesenchymal stem cell senescence through p53 and p16INK4a/pRb pathways,” PLoS One 8(3), e58314 (2013).
[Crossref] [PubMed]

J. Ren, D. F. Stroncek, Y. Zhao, P. Jin, L. Castiello, S. Civini, H. Wang, J. Feng, K. Tran, S. A. Kuznetsov, P. G. Robey, and M. Sabatino, “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence,” Stem Cell Res. (Amst.) 11(3), 1060–1073 (2013).
[Crossref] [PubMed]

J. C. Estrada, Y. Torres, A. Benguría, A. Dopazo, E. Roche, L. Carrera-Quintanar, R. A. Pérez, J. A. Enríquez, R. Torres, J. C. Ramírez, E. Samper, and A. Bernad, “Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy,” Cell Death Dis. 4(6), e691 (2013).
[Crossref] [PubMed]

F. C. Pascut, S. Kalra, V. George, N. Welch, C. Denning, and I. Notingher, “Non-invasive label-free monitoring the cardiac differentiation of human embryonic stem cells in-vitro by Raman spectroscopy,” Biochim. Biophys. Acta 1830(6), 3517–3524 (2013).
[Crossref] [PubMed]

S. O. Konorov, H. G. Schulze, J. M. Piret, M. W. Blades, and R. F. Turner, “Label-free determination of the cell cycle phase in human embryonic stem cells by Raman microspectroscopy,” Anal. Chem. 85(19), 8996–9002 (2013).
[Crossref] [PubMed]

2012 (2)

L. Harkness, S. M. Novikov, J. Beermann, S. I. Bozhevolnyi, and M. Kassem, “Identification of abnormal stem cells using Raman spectroscopy,” Stem Cells Dev. 21(12), 2152–2159 (2012).
[Crossref] [PubMed]

W. Gong, Z. Han, H. Zhao, Y. Wang, J. Wang, J. Zhong, B. Wang, S. Wang, Y. Wang, L. Sun, and Z. Han, “Banking human umbilical cord-derived mesenchymal stromal cells for clinical use,” Cell Transplant. 21(1), 207–216 (2012).
[Crossref] [PubMed]

2011 (7)

H. Cheng, L. Qiu, J. Ma, H. Zhang, M. Cheng, W. Li, X. Zhao, and K. Liu, “Replicative senescence of human bone marrow and umbilical cord derived mesenchymal stem cells and their differentiation to adipocytes and osteoblasts,” Mol. Biol. Rep. 38(8), 5161–5168 (2011).
[Crossref] [PubMed]

S. O. Konorov, H. G. Schulze, C. G. Atkins, J. M. Piret, S. A. Aparicio, R. F. B. Turner, and M. W. Blades, “Absolute quantification of intracellular glycogen content in human embryonic stem cells with Raman microspectroscopy,” Anal. Chem. 83(16), 6254–6258 (2011).
[Crossref] [PubMed]

M. Pudlas, D. A. C. Berrio, M. Votteler, S. Koch, S. Thude, H. Walles, and K. Schenke-Layland, “Non-contact discrimination of human bone marrow-derived mesenchymal stem cells and fibroblasts using Raman spectroscopy,” Med. Laser Appl. 26(3), 119–125 (2011).
[Crossref]

F. C. Pascut, H. T. Goh, V. George, C. Denning, and I. Notingher, “Toward label-free Raman-activated cell sorting of cardiomyocytes derived from human embryonic stem cells,” J. Biomed. Opt. 16(4), 045002 (2011).
[Crossref] [PubMed]

H. Bai, P. Chen, H. Fang, L. Lin, G. Q. Tang, G. G. Mu, W. Gong, Z. P. Liu, H. Wu, H. Zhao, and Z. C. Han, “Detecting viability transitions of umbilical cord mesenchymal stem cells by Raman micro-spectroscopy,” Laser Phys. Lett. 8(1), 78–84 (2011).
[Crossref]

H. Bai, P. Chen, G. Q. Tang, L. Lin, H. Fang, G. G. Mu, W. Gong, Z. P. Liu, Z. B. Han, H. Zhao, and Z. C. Han, “Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability,” Laser Phys. 21(6), 1122–1129 (2011).
[Crossref]

L. L. McManus, G. A. Burke, M. M. McCafferty, P. O’Hare, M. Modreanu, A. R. Boyd, and B. J. Meenan, “Raman spectroscopic monitoring of the osteogenic differentiation of human mesenchymal stem cells,” Analyst (Lond.) 136(12), 2471–2481 (2011).
[Crossref] [PubMed]

2010 (4)

H. G. Schulze, S. O. Konorov, N. J. Caron, J. M. Piret, M. W. Blades, and R. F. B. Turner, “Assessing differentiation status of human embryonic stem cells noninvasively using Raman microspectroscopy,” Anal. Chem. 82(12), 5020–5027 (2010).
[Crossref] [PubMed]

A. R. Boyd, G. A. Burke, and B. J. Meenan, “Monitoring cellular behaviour using Raman spectroscopy for tissue engineering and regenerative medicine applications,” J. Mater. Sci. Mater. Med. 21(8), 2317–2324 (2010).
[Crossref] [PubMed]

H. B. Noh, H. J. Ahn, W. J. Lee, K. B. Kwack, and Y. D. Kwon, “The molecular signature of in vitro senescence in human mesenchymal stem cells,” Genes Genomics 32(1), 87–93 (2010).
[Crossref]

H. Motaln, C. Schichor, and T. T. Lah, “Human mesenchymal stem cells and their use in cell-based therapies,” Cancer 116(11), 2519–2530 (2010).
[Crossref] [PubMed]

2009 (2)

J. W. Chan, D. K. Lieu, T. Huser, and R. A. Li, “Label-free separation of human embryonic stem cells and their cardiac derivatives using Raman spectroscopy,” Anal. Chem. 81(4), 1324–1331 (2009).
[Crossref] [PubMed]

H. K. Chiang, F. Y. Peng, S. C. Hung, and Y. C. Feng, “In situ Raman spectroscopic monitoring of hydroxyapatite as human mesenchymal stem cells differentiate into osteoblasts,” J. Raman Spectrosc. 40(5), 546–549 (2009).
[Crossref]

2008 (1)

W. Wagner, P. Horn, M. Castoldi, A. Diehlmann, S. Bork, R. Saffrich, V. Benes, J. Blake, S. Pfister, V. Eckstein, and A. D. Ho, “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PLoS One 3(5), e2213 (2008).
[Crossref] [PubMed]

2007 (1)

D. Baksh, R. Yao, and R. S. Tuan, “Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow,” Stem Cells 25(6), 1384–1392 (2007).
[Crossref] [PubMed]

2006 (1)

S. Sethe, A. Scutt, and A. Stolzing, “Aging of mesenchymal stem cells,” Ageing Res. Rev. 5(1), 91–116 (2006).
[Crossref] [PubMed]

2005 (2)

J. S. Park, H. Y. Kim, H. W. Kim, G. N. Chae, H. T. Oh, J. Y. Park, H. Shim, M. Seo, E. Y. Shin, E. G. Kim, S. C. Park, and S. J. Kwak, “Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells,” Mech. Ageing Dev. 126(5), 551–559 (2005).
[Crossref] [PubMed]

M. Terai, T. Uyama, T. Sugiki, X. K. Li, A. Umezawa, and T. Kiyono, “Immortalization of human fetal cells: the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway,” Mol. Biol. Cell 16(3), 1491–1499 (2005).
[Crossref] [PubMed]

2004 (2)

I. Notingher, I. Bisson, J. M. Polak, and L. L. Hench, “In situ spectroscopic study of nucleic acids in differentiating embryonic stem cells,” Vib. Spectrosc. 35(1-2), 199–203 (2004).
[Crossref]

H. S. Wang, S. C. Hung, S. T. Peng, C. C. Huang, H. M. Wei, Y. J. Guo, Y. S. Fu, M. C. Lai, and C. C. Chen, “Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord,” Stem Cells 22(7), 1330–1337 (2004).
[Crossref] [PubMed]

2003 (2)

K. E. Mitchell, M. L. Weiss, B. M. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, D. Troyer, and S. Medicetty, “Matrix cells from Wharton’s jelly form neurons and glia,” Stem Cells 21(1), 50–60 (2003).
[Crossref] [PubMed]

I. Notingher, S. Verrier, S. Haque, J. M. Polak, and L. L. Hench, “Spectroscopic study of human lung epithelial cells (A549) in culture: living cells versus dead cells,” Biopolymers 72(4), 230–240 (2003).
[Crossref] [PubMed]

Abou-Easa, K.

K. E. Mitchell, M. L. Weiss, B. M. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, D. Troyer, and S. Medicetty, “Matrix cells from Wharton’s jelly form neurons and glia,” Stem Cells 21(1), 50–60 (2003).
[Crossref] [PubMed]

Ahn, H. J.

H. B. Noh, H. J. Ahn, W. J. Lee, K. B. Kwack, and Y. D. Kwon, “The molecular signature of in vitro senescence in human mesenchymal stem cells,” Genes Genomics 32(1), 87–93 (2010).
[Crossref]

Aparicio, S. A.

S. O. Konorov, H. G. Schulze, C. G. Atkins, J. M. Piret, S. A. Aparicio, R. F. B. Turner, and M. W. Blades, “Absolute quantification of intracellular glycogen content in human embryonic stem cells with Raman microspectroscopy,” Anal. Chem. 83(16), 6254–6258 (2011).
[Crossref] [PubMed]

Atkins, C. G.

S. O. Konorov, H. G. Schulze, C. G. Atkins, J. M. Piret, S. A. Aparicio, R. F. B. Turner, and M. W. Blades, “Absolute quantification of intracellular glycogen content in human embryonic stem cells with Raman microspectroscopy,” Anal. Chem. 83(16), 6254–6258 (2011).
[Crossref] [PubMed]

Bai, H.

H. Bai, P. Chen, H. Fang, L. Lin, G. Q. Tang, G. G. Mu, W. Gong, Z. P. Liu, H. Wu, H. Zhao, and Z. C. Han, “Detecting viability transitions of umbilical cord mesenchymal stem cells by Raman micro-spectroscopy,” Laser Phys. Lett. 8(1), 78–84 (2011).
[Crossref]

H. Bai, P. Chen, G. Q. Tang, L. Lin, H. Fang, G. G. Mu, W. Gong, Z. P. Liu, Z. B. Han, H. Zhao, and Z. C. Han, “Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability,” Laser Phys. 21(6), 1122–1129 (2011).
[Crossref]

Baksh, D.

D. Baksh, R. Yao, and R. S. Tuan, “Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow,” Stem Cells 25(6), 1384–1392 (2007).
[Crossref] [PubMed]

Beerenstrauch, M.

K. E. Mitchell, M. L. Weiss, B. M. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, D. Troyer, and S. Medicetty, “Matrix cells from Wharton’s jelly form neurons and glia,” Stem Cells 21(1), 50–60 (2003).
[Crossref] [PubMed]

Beermann, J.

L. Harkness, S. M. Novikov, J. Beermann, S. I. Bozhevolnyi, and M. Kassem, “Identification of abnormal stem cells using Raman spectroscopy,” Stem Cells Dev. 21(12), 2152–2159 (2012).
[Crossref] [PubMed]

Benes, V.

W. Wagner, P. Horn, M. Castoldi, A. Diehlmann, S. Bork, R. Saffrich, V. Benes, J. Blake, S. Pfister, V. Eckstein, and A. D. Ho, “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PLoS One 3(5), e2213 (2008).
[Crossref] [PubMed]

Benguría, A.

J. C. Estrada, Y. Torres, A. Benguría, A. Dopazo, E. Roche, L. Carrera-Quintanar, R. A. Pérez, J. A. Enríquez, R. Torres, J. C. Ramírez, E. Samper, and A. Bernad, “Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy,” Cell Death Dis. 4(6), e691 (2013).
[Crossref] [PubMed]

Bernad, A.

J. C. Sepúlveda, M. Tomé, M. E. Fernández, M. Delgado, J. Campisi, A. Bernad, and M. A. González, “Cell senescence abrogates the therapeutic potential of human mesenchymal stem cells in the lethal endotoxemia model,” Stem Cells 32(7), 1865–1877 (2014).
[Crossref] [PubMed]

J. C. Estrada, Y. Torres, A. Benguría, A. Dopazo, E. Roche, L. Carrera-Quintanar, R. A. Pérez, J. A. Enríquez, R. Torres, J. C. Ramírez, E. Samper, and A. Bernad, “Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy,” Cell Death Dis. 4(6), e691 (2013).
[Crossref] [PubMed]

Berrio, D. A. C.

M. Pudlas, D. A. C. Berrio, M. Votteler, S. Koch, S. Thude, H. Walles, and K. Schenke-Layland, “Non-contact discrimination of human bone marrow-derived mesenchymal stem cells and fibroblasts using Raman spectroscopy,” Med. Laser Appl. 26(3), 119–125 (2011).
[Crossref]

Bisson, I.

I. Notingher, I. Bisson, J. M. Polak, and L. L. Hench, “In situ spectroscopic study of nucleic acids in differentiating embryonic stem cells,” Vib. Spectrosc. 35(1-2), 199–203 (2004).
[Crossref]

Blades, M. W.

S. O. Konorov, H. G. Schulze, J. M. Piret, M. W. Blades, and R. F. Turner, “Label-free determination of the cell cycle phase in human embryonic stem cells by Raman microspectroscopy,” Anal. Chem. 85(19), 8996–9002 (2013).
[Crossref] [PubMed]

S. O. Konorov, H. G. Schulze, C. G. Atkins, J. M. Piret, S. A. Aparicio, R. F. B. Turner, and M. W. Blades, “Absolute quantification of intracellular glycogen content in human embryonic stem cells with Raman microspectroscopy,” Anal. Chem. 83(16), 6254–6258 (2011).
[Crossref] [PubMed]

H. G. Schulze, S. O. Konorov, N. J. Caron, J. M. Piret, M. W. Blades, and R. F. B. Turner, “Assessing differentiation status of human embryonic stem cells noninvasively using Raman microspectroscopy,” Anal. Chem. 82(12), 5020–5027 (2010).
[Crossref] [PubMed]

Blake, J.

W. Wagner, P. Horn, M. Castoldi, A. Diehlmann, S. Bork, R. Saffrich, V. Benes, J. Blake, S. Pfister, V. Eckstein, and A. D. Ho, “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PLoS One 3(5), e2213 (2008).
[Crossref] [PubMed]

Bork, S.

W. Wagner, P. Horn, M. Castoldi, A. Diehlmann, S. Bork, R. Saffrich, V. Benes, J. Blake, S. Pfister, V. Eckstein, and A. D. Ho, “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PLoS One 3(5), e2213 (2008).
[Crossref] [PubMed]

Boyd, A. R.

L. L. McManus, G. A. Burke, M. M. McCafferty, P. O’Hare, M. Modreanu, A. R. Boyd, and B. J. Meenan, “Raman spectroscopic monitoring of the osteogenic differentiation of human mesenchymal stem cells,” Analyst (Lond.) 136(12), 2471–2481 (2011).
[Crossref] [PubMed]

A. R. Boyd, G. A. Burke, and B. J. Meenan, “Monitoring cellular behaviour using Raman spectroscopy for tissue engineering and regenerative medicine applications,” J. Mater. Sci. Mater. Med. 21(8), 2317–2324 (2010).
[Crossref] [PubMed]

Bozhevolnyi, S. I.

L. Harkness, S. M. Novikov, J. Beermann, S. I. Bozhevolnyi, and M. Kassem, “Identification of abnormal stem cells using Raman spectroscopy,” Stem Cells Dev. 21(12), 2152–2159 (2012).
[Crossref] [PubMed]

Burke, G. A.

L. L. McManus, G. A. Burke, M. M. McCafferty, P. O’Hare, M. Modreanu, A. R. Boyd, and B. J. Meenan, “Raman spectroscopic monitoring of the osteogenic differentiation of human mesenchymal stem cells,” Analyst (Lond.) 136(12), 2471–2481 (2011).
[Crossref] [PubMed]

A. R. Boyd, G. A. Burke, and B. J. Meenan, “Monitoring cellular behaviour using Raman spectroscopy for tissue engineering and regenerative medicine applications,” J. Mater. Sci. Mater. Med. 21(8), 2317–2324 (2010).
[Crossref] [PubMed]

Campisi, J.

J. C. Sepúlveda, M. Tomé, M. E. Fernández, M. Delgado, J. Campisi, A. Bernad, and M. A. González, “Cell senescence abrogates the therapeutic potential of human mesenchymal stem cells in the lethal endotoxemia model,” Stem Cells 32(7), 1865–1877 (2014).
[Crossref] [PubMed]

Caron, N. J.

H. G. Schulze, S. O. Konorov, N. J. Caron, J. M. Piret, M. W. Blades, and R. F. B. Turner, “Assessing differentiation status of human embryonic stem cells noninvasively using Raman microspectroscopy,” Anal. Chem. 82(12), 5020–5027 (2010).
[Crossref] [PubMed]

Carrera-Quintanar, L.

J. C. Estrada, Y. Torres, A. Benguría, A. Dopazo, E. Roche, L. Carrera-Quintanar, R. A. Pérez, J. A. Enríquez, R. Torres, J. C. Ramírez, E. Samper, and A. Bernad, “Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy,” Cell Death Dis. 4(6), e691 (2013).
[Crossref] [PubMed]

Castiello, L.

J. Ren, D. F. Stroncek, Y. Zhao, P. Jin, L. Castiello, S. Civini, H. Wang, J. Feng, K. Tran, S. A. Kuznetsov, P. G. Robey, and M. Sabatino, “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence,” Stem Cell Res. (Amst.) 11(3), 1060–1073 (2013).
[Crossref] [PubMed]

Castoldi, M.

W. Wagner, P. Horn, M. Castoldi, A. Diehlmann, S. Bork, R. Saffrich, V. Benes, J. Blake, S. Pfister, V. Eckstein, and A. D. Ho, “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PLoS One 3(5), e2213 (2008).
[Crossref] [PubMed]

Chae, G. N.

J. S. Park, H. Y. Kim, H. W. Kim, G. N. Chae, H. T. Oh, J. Y. Park, H. Shim, M. Seo, E. Y. Shin, E. G. Kim, S. C. Park, and S. J. Kwak, “Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells,” Mech. Ageing Dev. 126(5), 551–559 (2005).
[Crossref] [PubMed]

Chan, J. W.

J. W. Chan, D. K. Lieu, T. Huser, and R. A. Li, “Label-free separation of human embryonic stem cells and their cardiac derivatives using Raman spectroscopy,” Anal. Chem. 81(4), 1324–1331 (2009).
[Crossref] [PubMed]

Chen, C. C.

H. S. Wang, S. C. Hung, S. T. Peng, C. C. Huang, H. M. Wei, Y. J. Guo, Y. S. Fu, M. C. Lai, and C. C. Chen, “Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord,” Stem Cells 22(7), 1330–1337 (2004).
[Crossref] [PubMed]

Chen, G.

H. Hao, G. Chen, J. Liu, D. Ti, Y. Zhao, S. Xu, X. Fu, and W. Han, “Culturing on Wharton’s jelly extract delays mesenchymal stem cell senescence through p53 and p16INK4a/pRb pathways,” PLoS One 8(3), e58314 (2013).
[Crossref] [PubMed]

Chen, P.

H. Bai, P. Chen, G. Q. Tang, L. Lin, H. Fang, G. G. Mu, W. Gong, Z. P. Liu, Z. B. Han, H. Zhao, and Z. C. Han, “Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability,” Laser Phys. 21(6), 1122–1129 (2011).
[Crossref]

H. Bai, P. Chen, H. Fang, L. Lin, G. Q. Tang, G. G. Mu, W. Gong, Z. P. Liu, H. Wu, H. Zhao, and Z. C. Han, “Detecting viability transitions of umbilical cord mesenchymal stem cells by Raman micro-spectroscopy,” Laser Phys. Lett. 8(1), 78–84 (2011).
[Crossref]

Cheng, H.

H. Cheng, L. Qiu, J. Ma, H. Zhang, M. Cheng, W. Li, X. Zhao, and K. Liu, “Replicative senescence of human bone marrow and umbilical cord derived mesenchymal stem cells and their differentiation to adipocytes and osteoblasts,” Mol. Biol. Rep. 38(8), 5161–5168 (2011).
[Crossref] [PubMed]

Cheng, M.

H. Cheng, L. Qiu, J. Ma, H. Zhang, M. Cheng, W. Li, X. Zhao, and K. Liu, “Replicative senescence of human bone marrow and umbilical cord derived mesenchymal stem cells and their differentiation to adipocytes and osteoblasts,” Mol. Biol. Rep. 38(8), 5161–5168 (2011).
[Crossref] [PubMed]

Chiang, H. K.

H. K. Chiang, F. Y. Peng, S. C. Hung, and Y. C. Feng, “In situ Raman spectroscopic monitoring of hydroxyapatite as human mesenchymal stem cells differentiate into osteoblasts,” J. Raman Spectrosc. 40(5), 546–549 (2009).
[Crossref]

Civini, S.

J. Ren, D. F. Stroncek, Y. Zhao, P. Jin, L. Castiello, S. Civini, H. Wang, J. Feng, K. Tran, S. A. Kuznetsov, P. G. Robey, and M. Sabatino, “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence,” Stem Cell Res. (Amst.) 11(3), 1060–1073 (2013).
[Crossref] [PubMed]

Davis, D.

K. E. Mitchell, M. L. Weiss, B. M. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, D. Troyer, and S. Medicetty, “Matrix cells from Wharton’s jelly form neurons and glia,” Stem Cells 21(1), 50–60 (2003).
[Crossref] [PubMed]

Delgado, M.

J. C. Sepúlveda, M. Tomé, M. E. Fernández, M. Delgado, J. Campisi, A. Bernad, and M. A. González, “Cell senescence abrogates the therapeutic potential of human mesenchymal stem cells in the lethal endotoxemia model,” Stem Cells 32(7), 1865–1877 (2014).
[Crossref] [PubMed]

Denning, C.

F. C. Pascut, S. Kalra, V. George, N. Welch, C. Denning, and I. Notingher, “Non-invasive label-free monitoring the cardiac differentiation of human embryonic stem cells in-vitro by Raman spectroscopy,” Biochim. Biophys. Acta 1830(6), 3517–3524 (2013).
[Crossref] [PubMed]

F. C. Pascut, H. T. Goh, V. George, C. Denning, and I. Notingher, “Toward label-free Raman-activated cell sorting of cardiomyocytes derived from human embryonic stem cells,” J. Biomed. Opt. 16(4), 045002 (2011).
[Crossref] [PubMed]

Diehlmann, A.

W. Wagner, P. Horn, M. Castoldi, A. Diehlmann, S. Bork, R. Saffrich, V. Benes, J. Blake, S. Pfister, V. Eckstein, and A. D. Ho, “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PLoS One 3(5), e2213 (2008).
[Crossref] [PubMed]

Dopazo, A.

J. C. Estrada, Y. Torres, A. Benguría, A. Dopazo, E. Roche, L. Carrera-Quintanar, R. A. Pérez, J. A. Enríquez, R. Torres, J. C. Ramírez, E. Samper, and A. Bernad, “Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy,” Cell Death Dis. 4(6), e691 (2013).
[Crossref] [PubMed]

Eckstein, V.

W. Wagner, P. Horn, M. Castoldi, A. Diehlmann, S. Bork, R. Saffrich, V. Benes, J. Blake, S. Pfister, V. Eckstein, and A. D. Ho, “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PLoS One 3(5), e2213 (2008).
[Crossref] [PubMed]

Enríquez, J. A.

J. C. Estrada, Y. Torres, A. Benguría, A. Dopazo, E. Roche, L. Carrera-Quintanar, R. A. Pérez, J. A. Enríquez, R. Torres, J. C. Ramírez, E. Samper, and A. Bernad, “Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy,” Cell Death Dis. 4(6), e691 (2013).
[Crossref] [PubMed]

Estrada, J. C.

J. C. Estrada, Y. Torres, A. Benguría, A. Dopazo, E. Roche, L. Carrera-Quintanar, R. A. Pérez, J. A. Enríquez, R. Torres, J. C. Ramírez, E. Samper, and A. Bernad, “Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy,” Cell Death Dis. 4(6), e691 (2013).
[Crossref] [PubMed]

Fang, H.

H. Bai, P. Chen, G. Q. Tang, L. Lin, H. Fang, G. G. Mu, W. Gong, Z. P. Liu, Z. B. Han, H. Zhao, and Z. C. Han, “Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability,” Laser Phys. 21(6), 1122–1129 (2011).
[Crossref]

H. Bai, P. Chen, H. Fang, L. Lin, G. Q. Tang, G. G. Mu, W. Gong, Z. P. Liu, H. Wu, H. Zhao, and Z. C. Han, “Detecting viability transitions of umbilical cord mesenchymal stem cells by Raman micro-spectroscopy,” Laser Phys. Lett. 8(1), 78–84 (2011).
[Crossref]

Feng, J.

J. Ren, D. F. Stroncek, Y. Zhao, P. Jin, L. Castiello, S. Civini, H. Wang, J. Feng, K. Tran, S. A. Kuznetsov, P. G. Robey, and M. Sabatino, “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence,” Stem Cell Res. (Amst.) 11(3), 1060–1073 (2013).
[Crossref] [PubMed]

Feng, Y. C.

H. K. Chiang, F. Y. Peng, S. C. Hung, and Y. C. Feng, “In situ Raman spectroscopic monitoring of hydroxyapatite as human mesenchymal stem cells differentiate into osteoblasts,” J. Raman Spectrosc. 40(5), 546–549 (2009).
[Crossref]

Fernández, M. E.

J. C. Sepúlveda, M. Tomé, M. E. Fernández, M. Delgado, J. Campisi, A. Bernad, and M. A. González, “Cell senescence abrogates the therapeutic potential of human mesenchymal stem cells in the lethal endotoxemia model,” Stem Cells 32(7), 1865–1877 (2014).
[Crossref] [PubMed]

Fu, X.

H. Hao, G. Chen, J. Liu, D. Ti, Y. Zhao, S. Xu, X. Fu, and W. Han, “Culturing on Wharton’s jelly extract delays mesenchymal stem cell senescence through p53 and p16INK4a/pRb pathways,” PLoS One 8(3), e58314 (2013).
[Crossref] [PubMed]

Fu, Y. S.

H. S. Wang, S. C. Hung, S. T. Peng, C. C. Huang, H. M. Wei, Y. J. Guo, Y. S. Fu, M. C. Lai, and C. C. Chen, “Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord,” Stem Cells 22(7), 1330–1337 (2004).
[Crossref] [PubMed]

George, V.

F. C. Pascut, S. Kalra, V. George, N. Welch, C. Denning, and I. Notingher, “Non-invasive label-free monitoring the cardiac differentiation of human embryonic stem cells in-vitro by Raman spectroscopy,” Biochim. Biophys. Acta 1830(6), 3517–3524 (2013).
[Crossref] [PubMed]

F. C. Pascut, H. T. Goh, V. George, C. Denning, and I. Notingher, “Toward label-free Raman-activated cell sorting of cardiomyocytes derived from human embryonic stem cells,” J. Biomed. Opt. 16(4), 045002 (2011).
[Crossref] [PubMed]

Goh, H. T.

F. C. Pascut, H. T. Goh, V. George, C. Denning, and I. Notingher, “Toward label-free Raman-activated cell sorting of cardiomyocytes derived from human embryonic stem cells,” J. Biomed. Opt. 16(4), 045002 (2011).
[Crossref] [PubMed]

Gong, W.

W. Gong, Z. Han, H. Zhao, Y. Wang, J. Wang, J. Zhong, B. Wang, S. Wang, Y. Wang, L. Sun, and Z. Han, “Banking human umbilical cord-derived mesenchymal stromal cells for clinical use,” Cell Transplant. 21(1), 207–216 (2012).
[Crossref] [PubMed]

H. Bai, P. Chen, G. Q. Tang, L. Lin, H. Fang, G. G. Mu, W. Gong, Z. P. Liu, Z. B. Han, H. Zhao, and Z. C. Han, “Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability,” Laser Phys. 21(6), 1122–1129 (2011).
[Crossref]

H. Bai, P. Chen, H. Fang, L. Lin, G. Q. Tang, G. G. Mu, W. Gong, Z. P. Liu, H. Wu, H. Zhao, and Z. C. Han, “Detecting viability transitions of umbilical cord mesenchymal stem cells by Raman micro-spectroscopy,” Laser Phys. Lett. 8(1), 78–84 (2011).
[Crossref]

González, M. A.

J. C. Sepúlveda, M. Tomé, M. E. Fernández, M. Delgado, J. Campisi, A. Bernad, and M. A. González, “Cell senescence abrogates the therapeutic potential of human mesenchymal stem cells in the lethal endotoxemia model,” Stem Cells 32(7), 1865–1877 (2014).
[Crossref] [PubMed]

Guo, Y. J.

H. S. Wang, S. C. Hung, S. T. Peng, C. C. Huang, H. M. Wei, Y. J. Guo, Y. S. Fu, M. C. Lai, and C. C. Chen, “Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord,” Stem Cells 22(7), 1330–1337 (2004).
[Crossref] [PubMed]

Han, W.

H. Hao, G. Chen, J. Liu, D. Ti, Y. Zhao, S. Xu, X. Fu, and W. Han, “Culturing on Wharton’s jelly extract delays mesenchymal stem cell senescence through p53 and p16INK4a/pRb pathways,” PLoS One 8(3), e58314 (2013).
[Crossref] [PubMed]

Han, Z.

W. Gong, Z. Han, H. Zhao, Y. Wang, J. Wang, J. Zhong, B. Wang, S. Wang, Y. Wang, L. Sun, and Z. Han, “Banking human umbilical cord-derived mesenchymal stromal cells for clinical use,” Cell Transplant. 21(1), 207–216 (2012).
[Crossref] [PubMed]

W. Gong, Z. Han, H. Zhao, Y. Wang, J. Wang, J. Zhong, B. Wang, S. Wang, Y. Wang, L. Sun, and Z. Han, “Banking human umbilical cord-derived mesenchymal stromal cells for clinical use,” Cell Transplant. 21(1), 207–216 (2012).
[Crossref] [PubMed]

Han, Z. B.

H. Bai, P. Chen, G. Q. Tang, L. Lin, H. Fang, G. G. Mu, W. Gong, Z. P. Liu, Z. B. Han, H. Zhao, and Z. C. Han, “Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability,” Laser Phys. 21(6), 1122–1129 (2011).
[Crossref]

Han, Z. C.

H. Bai, P. Chen, H. Fang, L. Lin, G. Q. Tang, G. G. Mu, W. Gong, Z. P. Liu, H. Wu, H. Zhao, and Z. C. Han, “Detecting viability transitions of umbilical cord mesenchymal stem cells by Raman micro-spectroscopy,” Laser Phys. Lett. 8(1), 78–84 (2011).
[Crossref]

H. Bai, P. Chen, G. Q. Tang, L. Lin, H. Fang, G. G. Mu, W. Gong, Z. P. Liu, Z. B. Han, H. Zhao, and Z. C. Han, “Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability,” Laser Phys. 21(6), 1122–1129 (2011).
[Crossref]

Hao, H.

H. Hao, G. Chen, J. Liu, D. Ti, Y. Zhao, S. Xu, X. Fu, and W. Han, “Culturing on Wharton’s jelly extract delays mesenchymal stem cell senescence through p53 and p16INK4a/pRb pathways,” PLoS One 8(3), e58314 (2013).
[Crossref] [PubMed]

Haque, S.

I. Notingher, S. Verrier, S. Haque, J. M. Polak, and L. L. Hench, “Spectroscopic study of human lung epithelial cells (A549) in culture: living cells versus dead cells,” Biopolymers 72(4), 230–240 (2003).
[Crossref] [PubMed]

Harkness, L.

L. Harkness, S. M. Novikov, J. Beermann, S. I. Bozhevolnyi, and M. Kassem, “Identification of abnormal stem cells using Raman spectroscopy,” Stem Cells Dev. 21(12), 2152–2159 (2012).
[Crossref] [PubMed]

Helwig, B.

K. E. Mitchell, M. L. Weiss, B. M. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, D. Troyer, and S. Medicetty, “Matrix cells from Wharton’s jelly form neurons and glia,” Stem Cells 21(1), 50–60 (2003).
[Crossref] [PubMed]

Hench, L. L.

I. Notingher, I. Bisson, J. M. Polak, and L. L. Hench, “In situ spectroscopic study of nucleic acids in differentiating embryonic stem cells,” Vib. Spectrosc. 35(1-2), 199–203 (2004).
[Crossref]

I. Notingher, S. Verrier, S. Haque, J. M. Polak, and L. L. Hench, “Spectroscopic study of human lung epithelial cells (A549) in culture: living cells versus dead cells,” Biopolymers 72(4), 230–240 (2003).
[Crossref] [PubMed]

Hildreth, T.

K. E. Mitchell, M. L. Weiss, B. M. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, D. Troyer, and S. Medicetty, “Matrix cells from Wharton’s jelly form neurons and glia,” Stem Cells 21(1), 50–60 (2003).
[Crossref] [PubMed]

Ho, A. D.

W. Wagner, P. Horn, M. Castoldi, A. Diehlmann, S. Bork, R. Saffrich, V. Benes, J. Blake, S. Pfister, V. Eckstein, and A. D. Ho, “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PLoS One 3(5), e2213 (2008).
[Crossref] [PubMed]

Horn, P.

W. Wagner, P. Horn, M. Castoldi, A. Diehlmann, S. Bork, R. Saffrich, V. Benes, J. Blake, S. Pfister, V. Eckstein, and A. D. Ho, “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PLoS One 3(5), e2213 (2008).
[Crossref] [PubMed]

Huang, C. C.

H. S. Wang, S. C. Hung, S. T. Peng, C. C. Huang, H. M. Wei, Y. J. Guo, Y. S. Fu, M. C. Lai, and C. C. Chen, “Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord,” Stem Cells 22(7), 1330–1337 (2004).
[Crossref] [PubMed]

Hung, S. C.

H. K. Chiang, F. Y. Peng, S. C. Hung, and Y. C. Feng, “In situ Raman spectroscopic monitoring of hydroxyapatite as human mesenchymal stem cells differentiate into osteoblasts,” J. Raman Spectrosc. 40(5), 546–549 (2009).
[Crossref]

H. S. Wang, S. C. Hung, S. T. Peng, C. C. Huang, H. M. Wei, Y. J. Guo, Y. S. Fu, M. C. Lai, and C. C. Chen, “Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord,” Stem Cells 22(7), 1330–1337 (2004).
[Crossref] [PubMed]

Huser, T.

J. W. Chan, D. K. Lieu, T. Huser, and R. A. Li, “Label-free separation of human embryonic stem cells and their cardiac derivatives using Raman spectroscopy,” Anal. Chem. 81(4), 1324–1331 (2009).
[Crossref] [PubMed]

Jin, P.

J. Ren, D. F. Stroncek, Y. Zhao, P. Jin, L. Castiello, S. Civini, H. Wang, J. Feng, K. Tran, S. A. Kuznetsov, P. G. Robey, and M. Sabatino, “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence,” Stem Cell Res. (Amst.) 11(3), 1060–1073 (2013).
[Crossref] [PubMed]

Kalra, S.

F. C. Pascut, S. Kalra, V. George, N. Welch, C. Denning, and I. Notingher, “Non-invasive label-free monitoring the cardiac differentiation of human embryonic stem cells in-vitro by Raman spectroscopy,” Biochim. Biophys. Acta 1830(6), 3517–3524 (2013).
[Crossref] [PubMed]

Kassem, M.

L. Harkness, S. M. Novikov, J. Beermann, S. I. Bozhevolnyi, and M. Kassem, “Identification of abnormal stem cells using Raman spectroscopy,” Stem Cells Dev. 21(12), 2152–2159 (2012).
[Crossref] [PubMed]

Kim, E. G.

J. S. Park, H. Y. Kim, H. W. Kim, G. N. Chae, H. T. Oh, J. Y. Park, H. Shim, M. Seo, E. Y. Shin, E. G. Kim, S. C. Park, and S. J. Kwak, “Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells,” Mech. Ageing Dev. 126(5), 551–559 (2005).
[Crossref] [PubMed]

Kim, H. W.

J. S. Park, H. Y. Kim, H. W. Kim, G. N. Chae, H. T. Oh, J. Y. Park, H. Shim, M. Seo, E. Y. Shin, E. G. Kim, S. C. Park, and S. J. Kwak, “Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells,” Mech. Ageing Dev. 126(5), 551–559 (2005).
[Crossref] [PubMed]

Kim, H. Y.

J. S. Park, H. Y. Kim, H. W. Kim, G. N. Chae, H. T. Oh, J. Y. Park, H. Shim, M. Seo, E. Y. Shin, E. G. Kim, S. C. Park, and S. J. Kwak, “Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells,” Mech. Ageing Dev. 126(5), 551–559 (2005).
[Crossref] [PubMed]

Kiyono, T.

M. Terai, T. Uyama, T. Sugiki, X. K. Li, A. Umezawa, and T. Kiyono, “Immortalization of human fetal cells: the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway,” Mol. Biol. Cell 16(3), 1491–1499 (2005).
[Crossref] [PubMed]

Koch, S.

M. Pudlas, D. A. C. Berrio, M. Votteler, S. Koch, S. Thude, H. Walles, and K. Schenke-Layland, “Non-contact discrimination of human bone marrow-derived mesenchymal stem cells and fibroblasts using Raman spectroscopy,” Med. Laser Appl. 26(3), 119–125 (2011).
[Crossref]

Konorov, S. O.

S. O. Konorov, H. G. Schulze, J. M. Piret, M. W. Blades, and R. F. Turner, “Label-free determination of the cell cycle phase in human embryonic stem cells by Raman microspectroscopy,” Anal. Chem. 85(19), 8996–9002 (2013).
[Crossref] [PubMed]

S. O. Konorov, H. G. Schulze, C. G. Atkins, J. M. Piret, S. A. Aparicio, R. F. B. Turner, and M. W. Blades, “Absolute quantification of intracellular glycogen content in human embryonic stem cells with Raman microspectroscopy,” Anal. Chem. 83(16), 6254–6258 (2011).
[Crossref] [PubMed]

H. G. Schulze, S. O. Konorov, N. J. Caron, J. M. Piret, M. W. Blades, and R. F. B. Turner, “Assessing differentiation status of human embryonic stem cells noninvasively using Raman microspectroscopy,” Anal. Chem. 82(12), 5020–5027 (2010).
[Crossref] [PubMed]

Kuznetsov, S. A.

J. Ren, D. F. Stroncek, Y. Zhao, P. Jin, L. Castiello, S. Civini, H. Wang, J. Feng, K. Tran, S. A. Kuznetsov, P. G. Robey, and M. Sabatino, “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence,” Stem Cell Res. (Amst.) 11(3), 1060–1073 (2013).
[Crossref] [PubMed]

Kwack, K. B.

H. B. Noh, H. J. Ahn, W. J. Lee, K. B. Kwack, and Y. D. Kwon, “The molecular signature of in vitro senescence in human mesenchymal stem cells,” Genes Genomics 32(1), 87–93 (2010).
[Crossref]

Kwak, S. J.

J. S. Park, H. Y. Kim, H. W. Kim, G. N. Chae, H. T. Oh, J. Y. Park, H. Shim, M. Seo, E. Y. Shin, E. G. Kim, S. C. Park, and S. J. Kwak, “Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells,” Mech. Ageing Dev. 126(5), 551–559 (2005).
[Crossref] [PubMed]

Kwon, Y. D.

H. B. Noh, H. J. Ahn, W. J. Lee, K. B. Kwack, and Y. D. Kwon, “The molecular signature of in vitro senescence in human mesenchymal stem cells,” Genes Genomics 32(1), 87–93 (2010).
[Crossref]

Lah, T. T.

H. Motaln, C. Schichor, and T. T. Lah, “Human mesenchymal stem cells and their use in cell-based therapies,” Cancer 116(11), 2519–2530 (2010).
[Crossref] [PubMed]

Lai, M. C.

H. S. Wang, S. C. Hung, S. T. Peng, C. C. Huang, H. M. Wei, Y. J. Guo, Y. S. Fu, M. C. Lai, and C. C. Chen, “Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord,” Stem Cells 22(7), 1330–1337 (2004).
[Crossref] [PubMed]

Lechner, S.

M. C. Mitterberger, S. Lechner, M. Mattesich, and W. Zwerschke, “Adipogenic differentiation is impaired in replicative senescent human subcutaneous adipose-derived stromal/progenitor cells,” J. Gerontol. A Biol. Sci. Med. Sci. 69(1), 13–24 (2014).
[Crossref] [PubMed]

Lee, W. J.

H. B. Noh, H. J. Ahn, W. J. Lee, K. B. Kwack, and Y. D. Kwon, “The molecular signature of in vitro senescence in human mesenchymal stem cells,” Genes Genomics 32(1), 87–93 (2010).
[Crossref]

Li, R. A.

J. W. Chan, D. K. Lieu, T. Huser, and R. A. Li, “Label-free separation of human embryonic stem cells and their cardiac derivatives using Raman spectroscopy,” Anal. Chem. 81(4), 1324–1331 (2009).
[Crossref] [PubMed]

Li, W.

H. Cheng, L. Qiu, J. Ma, H. Zhang, M. Cheng, W. Li, X. Zhao, and K. Liu, “Replicative senescence of human bone marrow and umbilical cord derived mesenchymal stem cells and their differentiation to adipocytes and osteoblasts,” Mol. Biol. Rep. 38(8), 5161–5168 (2011).
[Crossref] [PubMed]

Li, X. K.

M. Terai, T. Uyama, T. Sugiki, X. K. Li, A. Umezawa, and T. Kiyono, “Immortalization of human fetal cells: the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway,” Mol. Biol. Cell 16(3), 1491–1499 (2005).
[Crossref] [PubMed]

Lieu, D. K.

J. W. Chan, D. K. Lieu, T. Huser, and R. A. Li, “Label-free separation of human embryonic stem cells and their cardiac derivatives using Raman spectroscopy,” Anal. Chem. 81(4), 1324–1331 (2009).
[Crossref] [PubMed]

Lin, L.

H. Bai, P. Chen, H. Fang, L. Lin, G. Q. Tang, G. G. Mu, W. Gong, Z. P. Liu, H. Wu, H. Zhao, and Z. C. Han, “Detecting viability transitions of umbilical cord mesenchymal stem cells by Raman micro-spectroscopy,” Laser Phys. Lett. 8(1), 78–84 (2011).
[Crossref]

H. Bai, P. Chen, G. Q. Tang, L. Lin, H. Fang, G. G. Mu, W. Gong, Z. P. Liu, Z. B. Han, H. Zhao, and Z. C. Han, “Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability,” Laser Phys. 21(6), 1122–1129 (2011).
[Crossref]

Liu, J.

H. Hao, G. Chen, J. Liu, D. Ti, Y. Zhao, S. Xu, X. Fu, and W. Han, “Culturing on Wharton’s jelly extract delays mesenchymal stem cell senescence through p53 and p16INK4a/pRb pathways,” PLoS One 8(3), e58314 (2013).
[Crossref] [PubMed]

Liu, K.

H. Cheng, L. Qiu, J. Ma, H. Zhang, M. Cheng, W. Li, X. Zhao, and K. Liu, “Replicative senescence of human bone marrow and umbilical cord derived mesenchymal stem cells and their differentiation to adipocytes and osteoblasts,” Mol. Biol. Rep. 38(8), 5161–5168 (2011).
[Crossref] [PubMed]

Liu, Z. P.

H. Bai, P. Chen, H. Fang, L. Lin, G. Q. Tang, G. G. Mu, W. Gong, Z. P. Liu, H. Wu, H. Zhao, and Z. C. Han, “Detecting viability transitions of umbilical cord mesenchymal stem cells by Raman micro-spectroscopy,” Laser Phys. Lett. 8(1), 78–84 (2011).
[Crossref]

H. Bai, P. Chen, G. Q. Tang, L. Lin, H. Fang, G. G. Mu, W. Gong, Z. P. Liu, Z. B. Han, H. Zhao, and Z. C. Han, “Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability,” Laser Phys. 21(6), 1122–1129 (2011).
[Crossref]

Ma, J.

H. Cheng, L. Qiu, J. Ma, H. Zhang, M. Cheng, W. Li, X. Zhao, and K. Liu, “Replicative senescence of human bone marrow and umbilical cord derived mesenchymal stem cells and their differentiation to adipocytes and osteoblasts,” Mol. Biol. Rep. 38(8), 5161–5168 (2011).
[Crossref] [PubMed]

Martin, P.

K. E. Mitchell, M. L. Weiss, B. M. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, D. Troyer, and S. Medicetty, “Matrix cells from Wharton’s jelly form neurons and glia,” Stem Cells 21(1), 50–60 (2003).
[Crossref] [PubMed]

Mattesich, M.

M. C. Mitterberger, S. Lechner, M. Mattesich, and W. Zwerschke, “Adipogenic differentiation is impaired in replicative senescent human subcutaneous adipose-derived stromal/progenitor cells,” J. Gerontol. A Biol. Sci. Med. Sci. 69(1), 13–24 (2014).
[Crossref] [PubMed]

McCafferty, M. M.

L. L. McManus, G. A. Burke, M. M. McCafferty, P. O’Hare, M. Modreanu, A. R. Boyd, and B. J. Meenan, “Raman spectroscopic monitoring of the osteogenic differentiation of human mesenchymal stem cells,” Analyst (Lond.) 136(12), 2471–2481 (2011).
[Crossref] [PubMed]

McManus, L. L.

L. L. McManus, G. A. Burke, M. M. McCafferty, P. O’Hare, M. Modreanu, A. R. Boyd, and B. J. Meenan, “Raman spectroscopic monitoring of the osteogenic differentiation of human mesenchymal stem cells,” Analyst (Lond.) 136(12), 2471–2481 (2011).
[Crossref] [PubMed]

Medicetty, S.

K. E. Mitchell, M. L. Weiss, B. M. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, D. Troyer, and S. Medicetty, “Matrix cells from Wharton’s jelly form neurons and glia,” Stem Cells 21(1), 50–60 (2003).
[Crossref] [PubMed]

Meenan, B. J.

L. L. McManus, G. A. Burke, M. M. McCafferty, P. O’Hare, M. Modreanu, A. R. Boyd, and B. J. Meenan, “Raman spectroscopic monitoring of the osteogenic differentiation of human mesenchymal stem cells,” Analyst (Lond.) 136(12), 2471–2481 (2011).
[Crossref] [PubMed]

A. R. Boyd, G. A. Burke, and B. J. Meenan, “Monitoring cellular behaviour using Raman spectroscopy for tissue engineering and regenerative medicine applications,” J. Mater. Sci. Mater. Med. 21(8), 2317–2324 (2010).
[Crossref] [PubMed]

Mitchell, B. M.

K. E. Mitchell, M. L. Weiss, B. M. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, D. Troyer, and S. Medicetty, “Matrix cells from Wharton’s jelly form neurons and glia,” Stem Cells 21(1), 50–60 (2003).
[Crossref] [PubMed]

Mitchell, K. E.

K. E. Mitchell, M. L. Weiss, B. M. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, D. Troyer, and S. Medicetty, “Matrix cells from Wharton’s jelly form neurons and glia,” Stem Cells 21(1), 50–60 (2003).
[Crossref] [PubMed]

Mitterberger, M. C.

M. C. Mitterberger, S. Lechner, M. Mattesich, and W. Zwerschke, “Adipogenic differentiation is impaired in replicative senescent human subcutaneous adipose-derived stromal/progenitor cells,” J. Gerontol. A Biol. Sci. Med. Sci. 69(1), 13–24 (2014).
[Crossref] [PubMed]

Modreanu, M.

L. L. McManus, G. A. Burke, M. M. McCafferty, P. O’Hare, M. Modreanu, A. R. Boyd, and B. J. Meenan, “Raman spectroscopic monitoring of the osteogenic differentiation of human mesenchymal stem cells,” Analyst (Lond.) 136(12), 2471–2481 (2011).
[Crossref] [PubMed]

Morales, L.

K. E. Mitchell, M. L. Weiss, B. M. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, D. Troyer, and S. Medicetty, “Matrix cells from Wharton’s jelly form neurons and glia,” Stem Cells 21(1), 50–60 (2003).
[Crossref] [PubMed]

Motaln, H.

H. Motaln, C. Schichor, and T. T. Lah, “Human mesenchymal stem cells and their use in cell-based therapies,” Cancer 116(11), 2519–2530 (2010).
[Crossref] [PubMed]

Mu, G. G.

H. Bai, P. Chen, G. Q. Tang, L. Lin, H. Fang, G. G. Mu, W. Gong, Z. P. Liu, Z. B. Han, H. Zhao, and Z. C. Han, “Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability,” Laser Phys. 21(6), 1122–1129 (2011).
[Crossref]

H. Bai, P. Chen, H. Fang, L. Lin, G. Q. Tang, G. G. Mu, W. Gong, Z. P. Liu, H. Wu, H. Zhao, and Z. C. Han, “Detecting viability transitions of umbilical cord mesenchymal stem cells by Raman micro-spectroscopy,” Laser Phys. Lett. 8(1), 78–84 (2011).
[Crossref]

Noh, H. B.

H. B. Noh, H. J. Ahn, W. J. Lee, K. B. Kwack, and Y. D. Kwon, “The molecular signature of in vitro senescence in human mesenchymal stem cells,” Genes Genomics 32(1), 87–93 (2010).
[Crossref]

Notingher, I.

F. C. Pascut, S. Kalra, V. George, N. Welch, C. Denning, and I. Notingher, “Non-invasive label-free monitoring the cardiac differentiation of human embryonic stem cells in-vitro by Raman spectroscopy,” Biochim. Biophys. Acta 1830(6), 3517–3524 (2013).
[Crossref] [PubMed]

F. C. Pascut, H. T. Goh, V. George, C. Denning, and I. Notingher, “Toward label-free Raman-activated cell sorting of cardiomyocytes derived from human embryonic stem cells,” J. Biomed. Opt. 16(4), 045002 (2011).
[Crossref] [PubMed]

I. Notingher, I. Bisson, J. M. Polak, and L. L. Hench, “In situ spectroscopic study of nucleic acids in differentiating embryonic stem cells,” Vib. Spectrosc. 35(1-2), 199–203 (2004).
[Crossref]

I. Notingher, S. Verrier, S. Haque, J. M. Polak, and L. L. Hench, “Spectroscopic study of human lung epithelial cells (A549) in culture: living cells versus dead cells,” Biopolymers 72(4), 230–240 (2003).
[Crossref] [PubMed]

Novikov, S. M.

L. Harkness, S. M. Novikov, J. Beermann, S. I. Bozhevolnyi, and M. Kassem, “Identification of abnormal stem cells using Raman spectroscopy,” Stem Cells Dev. 21(12), 2152–2159 (2012).
[Crossref] [PubMed]

O’Hare, P.

L. L. McManus, G. A. Burke, M. M. McCafferty, P. O’Hare, M. Modreanu, A. R. Boyd, and B. J. Meenan, “Raman spectroscopic monitoring of the osteogenic differentiation of human mesenchymal stem cells,” Analyst (Lond.) 136(12), 2471–2481 (2011).
[Crossref] [PubMed]

Oh, H. T.

J. S. Park, H. Y. Kim, H. W. Kim, G. N. Chae, H. T. Oh, J. Y. Park, H. Shim, M. Seo, E. Y. Shin, E. G. Kim, S. C. Park, and S. J. Kwak, “Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells,” Mech. Ageing Dev. 126(5), 551–559 (2005).
[Crossref] [PubMed]

Park, J. S.

J. S. Park, H. Y. Kim, H. W. Kim, G. N. Chae, H. T. Oh, J. Y. Park, H. Shim, M. Seo, E. Y. Shin, E. G. Kim, S. C. Park, and S. J. Kwak, “Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells,” Mech. Ageing Dev. 126(5), 551–559 (2005).
[Crossref] [PubMed]

Park, J. Y.

J. S. Park, H. Y. Kim, H. W. Kim, G. N. Chae, H. T. Oh, J. Y. Park, H. Shim, M. Seo, E. Y. Shin, E. G. Kim, S. C. Park, and S. J. Kwak, “Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells,” Mech. Ageing Dev. 126(5), 551–559 (2005).
[Crossref] [PubMed]

Park, S. C.

J. S. Park, H. Y. Kim, H. W. Kim, G. N. Chae, H. T. Oh, J. Y. Park, H. Shim, M. Seo, E. Y. Shin, E. G. Kim, S. C. Park, and S. J. Kwak, “Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells,” Mech. Ageing Dev. 126(5), 551–559 (2005).
[Crossref] [PubMed]

Pascut, F. C.

F. C. Pascut, S. Kalra, V. George, N. Welch, C. Denning, and I. Notingher, “Non-invasive label-free monitoring the cardiac differentiation of human embryonic stem cells in-vitro by Raman spectroscopy,” Biochim. Biophys. Acta 1830(6), 3517–3524 (2013).
[Crossref] [PubMed]

F. C. Pascut, H. T. Goh, V. George, C. Denning, and I. Notingher, “Toward label-free Raman-activated cell sorting of cardiomyocytes derived from human embryonic stem cells,” J. Biomed. Opt. 16(4), 045002 (2011).
[Crossref] [PubMed]

Peng, F. Y.

H. K. Chiang, F. Y. Peng, S. C. Hung, and Y. C. Feng, “In situ Raman spectroscopic monitoring of hydroxyapatite as human mesenchymal stem cells differentiate into osteoblasts,” J. Raman Spectrosc. 40(5), 546–549 (2009).
[Crossref]

Peng, S. T.

H. S. Wang, S. C. Hung, S. T. Peng, C. C. Huang, H. M. Wei, Y. J. Guo, Y. S. Fu, M. C. Lai, and C. C. Chen, “Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord,” Stem Cells 22(7), 1330–1337 (2004).
[Crossref] [PubMed]

Pérez, R. A.

J. C. Estrada, Y. Torres, A. Benguría, A. Dopazo, E. Roche, L. Carrera-Quintanar, R. A. Pérez, J. A. Enríquez, R. Torres, J. C. Ramírez, E. Samper, and A. Bernad, “Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy,” Cell Death Dis. 4(6), e691 (2013).
[Crossref] [PubMed]

Pfister, S.

W. Wagner, P. Horn, M. Castoldi, A. Diehlmann, S. Bork, R. Saffrich, V. Benes, J. Blake, S. Pfister, V. Eckstein, and A. D. Ho, “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PLoS One 3(5), e2213 (2008).
[Crossref] [PubMed]

Piret, J. M.

S. O. Konorov, H. G. Schulze, J. M. Piret, M. W. Blades, and R. F. Turner, “Label-free determination of the cell cycle phase in human embryonic stem cells by Raman microspectroscopy,” Anal. Chem. 85(19), 8996–9002 (2013).
[Crossref] [PubMed]

S. O. Konorov, H. G. Schulze, C. G. Atkins, J. M. Piret, S. A. Aparicio, R. F. B. Turner, and M. W. Blades, “Absolute quantification of intracellular glycogen content in human embryonic stem cells with Raman microspectroscopy,” Anal. Chem. 83(16), 6254–6258 (2011).
[Crossref] [PubMed]

H. G. Schulze, S. O. Konorov, N. J. Caron, J. M. Piret, M. W. Blades, and R. F. B. Turner, “Assessing differentiation status of human embryonic stem cells noninvasively using Raman microspectroscopy,” Anal. Chem. 82(12), 5020–5027 (2010).
[Crossref] [PubMed]

Polak, J. M.

I. Notingher, I. Bisson, J. M. Polak, and L. L. Hench, “In situ spectroscopic study of nucleic acids in differentiating embryonic stem cells,” Vib. Spectrosc. 35(1-2), 199–203 (2004).
[Crossref]

I. Notingher, S. Verrier, S. Haque, J. M. Polak, and L. L. Hench, “Spectroscopic study of human lung epithelial cells (A549) in culture: living cells versus dead cells,” Biopolymers 72(4), 230–240 (2003).
[Crossref] [PubMed]

Pudlas, M.

M. Pudlas, D. A. C. Berrio, M. Votteler, S. Koch, S. Thude, H. Walles, and K. Schenke-Layland, “Non-contact discrimination of human bone marrow-derived mesenchymal stem cells and fibroblasts using Raman spectroscopy,” Med. Laser Appl. 26(3), 119–125 (2011).
[Crossref]

Qiu, L.

H. Cheng, L. Qiu, J. Ma, H. Zhang, M. Cheng, W. Li, X. Zhao, and K. Liu, “Replicative senescence of human bone marrow and umbilical cord derived mesenchymal stem cells and their differentiation to adipocytes and osteoblasts,” Mol. Biol. Rep. 38(8), 5161–5168 (2011).
[Crossref] [PubMed]

Ramírez, J. C.

J. C. Estrada, Y. Torres, A. Benguría, A. Dopazo, E. Roche, L. Carrera-Quintanar, R. A. Pérez, J. A. Enríquez, R. Torres, J. C. Ramírez, E. Samper, and A. Bernad, “Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy,” Cell Death Dis. 4(6), e691 (2013).
[Crossref] [PubMed]

Ren, J.

J. Ren, D. F. Stroncek, Y. Zhao, P. Jin, L. Castiello, S. Civini, H. Wang, J. Feng, K. Tran, S. A. Kuznetsov, P. G. Robey, and M. Sabatino, “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence,” Stem Cell Res. (Amst.) 11(3), 1060–1073 (2013).
[Crossref] [PubMed]

Robey, P. G.

J. Ren, D. F. Stroncek, Y. Zhao, P. Jin, L. Castiello, S. Civini, H. Wang, J. Feng, K. Tran, S. A. Kuznetsov, P. G. Robey, and M. Sabatino, “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence,” Stem Cell Res. (Amst.) 11(3), 1060–1073 (2013).
[Crossref] [PubMed]

Roche, E.

J. C. Estrada, Y. Torres, A. Benguría, A. Dopazo, E. Roche, L. Carrera-Quintanar, R. A. Pérez, J. A. Enríquez, R. Torres, J. C. Ramírez, E. Samper, and A. Bernad, “Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy,” Cell Death Dis. 4(6), e691 (2013).
[Crossref] [PubMed]

Sabatino, M.

J. Ren, D. F. Stroncek, Y. Zhao, P. Jin, L. Castiello, S. Civini, H. Wang, J. Feng, K. Tran, S. A. Kuznetsov, P. G. Robey, and M. Sabatino, “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence,” Stem Cell Res. (Amst.) 11(3), 1060–1073 (2013).
[Crossref] [PubMed]

Saffrich, R.

W. Wagner, P. Horn, M. Castoldi, A. Diehlmann, S. Bork, R. Saffrich, V. Benes, J. Blake, S. Pfister, V. Eckstein, and A. D. Ho, “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PLoS One 3(5), e2213 (2008).
[Crossref] [PubMed]

Samper, E.

J. C. Estrada, Y. Torres, A. Benguría, A. Dopazo, E. Roche, L. Carrera-Quintanar, R. A. Pérez, J. A. Enríquez, R. Torres, J. C. Ramírez, E. Samper, and A. Bernad, “Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy,” Cell Death Dis. 4(6), e691 (2013).
[Crossref] [PubMed]

Schenke-Layland, K.

M. Pudlas, D. A. C. Berrio, M. Votteler, S. Koch, S. Thude, H. Walles, and K. Schenke-Layland, “Non-contact discrimination of human bone marrow-derived mesenchymal stem cells and fibroblasts using Raman spectroscopy,” Med. Laser Appl. 26(3), 119–125 (2011).
[Crossref]

Schichor, C.

H. Motaln, C. Schichor, and T. T. Lah, “Human mesenchymal stem cells and their use in cell-based therapies,” Cancer 116(11), 2519–2530 (2010).
[Crossref] [PubMed]

Schulze, H. G.

S. O. Konorov, H. G. Schulze, J. M. Piret, M. W. Blades, and R. F. Turner, “Label-free determination of the cell cycle phase in human embryonic stem cells by Raman microspectroscopy,” Anal. Chem. 85(19), 8996–9002 (2013).
[Crossref] [PubMed]

S. O. Konorov, H. G. Schulze, C. G. Atkins, J. M. Piret, S. A. Aparicio, R. F. B. Turner, and M. W. Blades, “Absolute quantification of intracellular glycogen content in human embryonic stem cells with Raman microspectroscopy,” Anal. Chem. 83(16), 6254–6258 (2011).
[Crossref] [PubMed]

H. G. Schulze, S. O. Konorov, N. J. Caron, J. M. Piret, M. W. Blades, and R. F. B. Turner, “Assessing differentiation status of human embryonic stem cells noninvasively using Raman microspectroscopy,” Anal. Chem. 82(12), 5020–5027 (2010).
[Crossref] [PubMed]

Scutt, A.

S. Sethe, A. Scutt, and A. Stolzing, “Aging of mesenchymal stem cells,” Ageing Res. Rev. 5(1), 91–116 (2006).
[Crossref] [PubMed]

Seo, M.

J. S. Park, H. Y. Kim, H. W. Kim, G. N. Chae, H. T. Oh, J. Y. Park, H. Shim, M. Seo, E. Y. Shin, E. G. Kim, S. C. Park, and S. J. Kwak, “Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells,” Mech. Ageing Dev. 126(5), 551–559 (2005).
[Crossref] [PubMed]

Sepúlveda, J. C.

J. C. Sepúlveda, M. Tomé, M. E. Fernández, M. Delgado, J. Campisi, A. Bernad, and M. A. González, “Cell senescence abrogates the therapeutic potential of human mesenchymal stem cells in the lethal endotoxemia model,” Stem Cells 32(7), 1865–1877 (2014).
[Crossref] [PubMed]

Sethe, S.

S. Sethe, A. Scutt, and A. Stolzing, “Aging of mesenchymal stem cells,” Ageing Res. Rev. 5(1), 91–116 (2006).
[Crossref] [PubMed]

Shim, H.

J. S. Park, H. Y. Kim, H. W. Kim, G. N. Chae, H. T. Oh, J. Y. Park, H. Shim, M. Seo, E. Y. Shin, E. G. Kim, S. C. Park, and S. J. Kwak, “Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells,” Mech. Ageing Dev. 126(5), 551–559 (2005).
[Crossref] [PubMed]

Shin, E. Y.

J. S. Park, H. Y. Kim, H. W. Kim, G. N. Chae, H. T. Oh, J. Y. Park, H. Shim, M. Seo, E. Y. Shin, E. G. Kim, S. C. Park, and S. J. Kwak, “Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells,” Mech. Ageing Dev. 126(5), 551–559 (2005).
[Crossref] [PubMed]

Stolzing, A.

S. Sethe, A. Scutt, and A. Stolzing, “Aging of mesenchymal stem cells,” Ageing Res. Rev. 5(1), 91–116 (2006).
[Crossref] [PubMed]

Stroncek, D. F.

J. Ren, D. F. Stroncek, Y. Zhao, P. Jin, L. Castiello, S. Civini, H. Wang, J. Feng, K. Tran, S. A. Kuznetsov, P. G. Robey, and M. Sabatino, “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence,” Stem Cell Res. (Amst.) 11(3), 1060–1073 (2013).
[Crossref] [PubMed]

Sugiki, T.

M. Terai, T. Uyama, T. Sugiki, X. K. Li, A. Umezawa, and T. Kiyono, “Immortalization of human fetal cells: the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway,” Mol. Biol. Cell 16(3), 1491–1499 (2005).
[Crossref] [PubMed]

Sun, L.

W. Gong, Z. Han, H. Zhao, Y. Wang, J. Wang, J. Zhong, B. Wang, S. Wang, Y. Wang, L. Sun, and Z. Han, “Banking human umbilical cord-derived mesenchymal stromal cells for clinical use,” Cell Transplant. 21(1), 207–216 (2012).
[Crossref] [PubMed]

Tang, G. Q.

H. Bai, P. Chen, G. Q. Tang, L. Lin, H. Fang, G. G. Mu, W. Gong, Z. P. Liu, Z. B. Han, H. Zhao, and Z. C. Han, “Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability,” Laser Phys. 21(6), 1122–1129 (2011).
[Crossref]

H. Bai, P. Chen, H. Fang, L. Lin, G. Q. Tang, G. G. Mu, W. Gong, Z. P. Liu, H. Wu, H. Zhao, and Z. C. Han, “Detecting viability transitions of umbilical cord mesenchymal stem cells by Raman micro-spectroscopy,” Laser Phys. Lett. 8(1), 78–84 (2011).
[Crossref]

Terai, M.

M. Terai, T. Uyama, T. Sugiki, X. K. Li, A. Umezawa, and T. Kiyono, “Immortalization of human fetal cells: the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway,” Mol. Biol. Cell 16(3), 1491–1499 (2005).
[Crossref] [PubMed]

Thude, S.

M. Pudlas, D. A. C. Berrio, M. Votteler, S. Koch, S. Thude, H. Walles, and K. Schenke-Layland, “Non-contact discrimination of human bone marrow-derived mesenchymal stem cells and fibroblasts using Raman spectroscopy,” Med. Laser Appl. 26(3), 119–125 (2011).
[Crossref]

Ti, D.

H. Hao, G. Chen, J. Liu, D. Ti, Y. Zhao, S. Xu, X. Fu, and W. Han, “Culturing on Wharton’s jelly extract delays mesenchymal stem cell senescence through p53 and p16INK4a/pRb pathways,” PLoS One 8(3), e58314 (2013).
[Crossref] [PubMed]

Tomé, M.

J. C. Sepúlveda, M. Tomé, M. E. Fernández, M. Delgado, J. Campisi, A. Bernad, and M. A. González, “Cell senescence abrogates the therapeutic potential of human mesenchymal stem cells in the lethal endotoxemia model,” Stem Cells 32(7), 1865–1877 (2014).
[Crossref] [PubMed]

Torres, R.

J. C. Estrada, Y. Torres, A. Benguría, A. Dopazo, E. Roche, L. Carrera-Quintanar, R. A. Pérez, J. A. Enríquez, R. Torres, J. C. Ramírez, E. Samper, and A. Bernad, “Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy,” Cell Death Dis. 4(6), e691 (2013).
[Crossref] [PubMed]

Torres, Y.

J. C. Estrada, Y. Torres, A. Benguría, A. Dopazo, E. Roche, L. Carrera-Quintanar, R. A. Pérez, J. A. Enríquez, R. Torres, J. C. Ramírez, E. Samper, and A. Bernad, “Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy,” Cell Death Dis. 4(6), e691 (2013).
[Crossref] [PubMed]

Tran, K.

J. Ren, D. F. Stroncek, Y. Zhao, P. Jin, L. Castiello, S. Civini, H. Wang, J. Feng, K. Tran, S. A. Kuznetsov, P. G. Robey, and M. Sabatino, “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence,” Stem Cell Res. (Amst.) 11(3), 1060–1073 (2013).
[Crossref] [PubMed]

Troyer, D.

K. E. Mitchell, M. L. Weiss, B. M. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, D. Troyer, and S. Medicetty, “Matrix cells from Wharton’s jelly form neurons and glia,” Stem Cells 21(1), 50–60 (2003).
[Crossref] [PubMed]

Tuan, R. S.

D. Baksh, R. Yao, and R. S. Tuan, “Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow,” Stem Cells 25(6), 1384–1392 (2007).
[Crossref] [PubMed]

Turner, R. F.

S. O. Konorov, H. G. Schulze, J. M. Piret, M. W. Blades, and R. F. Turner, “Label-free determination of the cell cycle phase in human embryonic stem cells by Raman microspectroscopy,” Anal. Chem. 85(19), 8996–9002 (2013).
[Crossref] [PubMed]

Turner, R. F. B.

S. O. Konorov, H. G. Schulze, C. G. Atkins, J. M. Piret, S. A. Aparicio, R. F. B. Turner, and M. W. Blades, “Absolute quantification of intracellular glycogen content in human embryonic stem cells with Raman microspectroscopy,” Anal. Chem. 83(16), 6254–6258 (2011).
[Crossref] [PubMed]

H. G. Schulze, S. O. Konorov, N. J. Caron, J. M. Piret, M. W. Blades, and R. F. B. Turner, “Assessing differentiation status of human embryonic stem cells noninvasively using Raman microspectroscopy,” Anal. Chem. 82(12), 5020–5027 (2010).
[Crossref] [PubMed]

Umezawa, A.

M. Terai, T. Uyama, T. Sugiki, X. K. Li, A. Umezawa, and T. Kiyono, “Immortalization of human fetal cells: the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway,” Mol. Biol. Cell 16(3), 1491–1499 (2005).
[Crossref] [PubMed]

Uyama, T.

M. Terai, T. Uyama, T. Sugiki, X. K. Li, A. Umezawa, and T. Kiyono, “Immortalization of human fetal cells: the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway,” Mol. Biol. Cell 16(3), 1491–1499 (2005).
[Crossref] [PubMed]

Verrier, S.

I. Notingher, S. Verrier, S. Haque, J. M. Polak, and L. L. Hench, “Spectroscopic study of human lung epithelial cells (A549) in culture: living cells versus dead cells,” Biopolymers 72(4), 230–240 (2003).
[Crossref] [PubMed]

Votteler, M.

M. Pudlas, D. A. C. Berrio, M. Votteler, S. Koch, S. Thude, H. Walles, and K. Schenke-Layland, “Non-contact discrimination of human bone marrow-derived mesenchymal stem cells and fibroblasts using Raman spectroscopy,” Med. Laser Appl. 26(3), 119–125 (2011).
[Crossref]

Wagner, W.

W. Wagner, P. Horn, M. Castoldi, A. Diehlmann, S. Bork, R. Saffrich, V. Benes, J. Blake, S. Pfister, V. Eckstein, and A. D. Ho, “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PLoS One 3(5), e2213 (2008).
[Crossref] [PubMed]

Walles, H.

M. Pudlas, D. A. C. Berrio, M. Votteler, S. Koch, S. Thude, H. Walles, and K. Schenke-Layland, “Non-contact discrimination of human bone marrow-derived mesenchymal stem cells and fibroblasts using Raman spectroscopy,” Med. Laser Appl. 26(3), 119–125 (2011).
[Crossref]

Wang, B.

W. Gong, Z. Han, H. Zhao, Y. Wang, J. Wang, J. Zhong, B. Wang, S. Wang, Y. Wang, L. Sun, and Z. Han, “Banking human umbilical cord-derived mesenchymal stromal cells for clinical use,” Cell Transplant. 21(1), 207–216 (2012).
[Crossref] [PubMed]

Wang, H.

J. Ren, D. F. Stroncek, Y. Zhao, P. Jin, L. Castiello, S. Civini, H. Wang, J. Feng, K. Tran, S. A. Kuznetsov, P. G. Robey, and M. Sabatino, “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence,” Stem Cell Res. (Amst.) 11(3), 1060–1073 (2013).
[Crossref] [PubMed]

Wang, H. S.

H. S. Wang, S. C. Hung, S. T. Peng, C. C. Huang, H. M. Wei, Y. J. Guo, Y. S. Fu, M. C. Lai, and C. C. Chen, “Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord,” Stem Cells 22(7), 1330–1337 (2004).
[Crossref] [PubMed]

Wang, J.

W. Gong, Z. Han, H. Zhao, Y. Wang, J. Wang, J. Zhong, B. Wang, S. Wang, Y. Wang, L. Sun, and Z. Han, “Banking human umbilical cord-derived mesenchymal stromal cells for clinical use,” Cell Transplant. 21(1), 207–216 (2012).
[Crossref] [PubMed]

Wang, S.

W. Gong, Z. Han, H. Zhao, Y. Wang, J. Wang, J. Zhong, B. Wang, S. Wang, Y. Wang, L. Sun, and Z. Han, “Banking human umbilical cord-derived mesenchymal stromal cells for clinical use,” Cell Transplant. 21(1), 207–216 (2012).
[Crossref] [PubMed]

Wang, Y.

W. Gong, Z. Han, H. Zhao, Y. Wang, J. Wang, J. Zhong, B. Wang, S. Wang, Y. Wang, L. Sun, and Z. Han, “Banking human umbilical cord-derived mesenchymal stromal cells for clinical use,” Cell Transplant. 21(1), 207–216 (2012).
[Crossref] [PubMed]

W. Gong, Z. Han, H. Zhao, Y. Wang, J. Wang, J. Zhong, B. Wang, S. Wang, Y. Wang, L. Sun, and Z. Han, “Banking human umbilical cord-derived mesenchymal stromal cells for clinical use,” Cell Transplant. 21(1), 207–216 (2012).
[Crossref] [PubMed]

Wei, H. M.

H. S. Wang, S. C. Hung, S. T. Peng, C. C. Huang, H. M. Wei, Y. J. Guo, Y. S. Fu, M. C. Lai, and C. C. Chen, “Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord,” Stem Cells 22(7), 1330–1337 (2004).
[Crossref] [PubMed]

Weiss, M. L.

K. E. Mitchell, M. L. Weiss, B. M. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, D. Troyer, and S. Medicetty, “Matrix cells from Wharton’s jelly form neurons and glia,” Stem Cells 21(1), 50–60 (2003).
[Crossref] [PubMed]

Welch, N.

F. C. Pascut, S. Kalra, V. George, N. Welch, C. Denning, and I. Notingher, “Non-invasive label-free monitoring the cardiac differentiation of human embryonic stem cells in-vitro by Raman spectroscopy,” Biochim. Biophys. Acta 1830(6), 3517–3524 (2013).
[Crossref] [PubMed]

Wu, H.

H. Bai, P. Chen, H. Fang, L. Lin, G. Q. Tang, G. G. Mu, W. Gong, Z. P. Liu, H. Wu, H. Zhao, and Z. C. Han, “Detecting viability transitions of umbilical cord mesenchymal stem cells by Raman micro-spectroscopy,” Laser Phys. Lett. 8(1), 78–84 (2011).
[Crossref]

Xu, S.

H. Hao, G. Chen, J. Liu, D. Ti, Y. Zhao, S. Xu, X. Fu, and W. Han, “Culturing on Wharton’s jelly extract delays mesenchymal stem cell senescence through p53 and p16INK4a/pRb pathways,” PLoS One 8(3), e58314 (2013).
[Crossref] [PubMed]

Yao, R.

D. Baksh, R. Yao, and R. S. Tuan, “Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow,” Stem Cells 25(6), 1384–1392 (2007).
[Crossref] [PubMed]

Zhang, H.

H. Cheng, L. Qiu, J. Ma, H. Zhang, M. Cheng, W. Li, X. Zhao, and K. Liu, “Replicative senescence of human bone marrow and umbilical cord derived mesenchymal stem cells and their differentiation to adipocytes and osteoblasts,” Mol. Biol. Rep. 38(8), 5161–5168 (2011).
[Crossref] [PubMed]

Zhao, H.

W. Gong, Z. Han, H. Zhao, Y. Wang, J. Wang, J. Zhong, B. Wang, S. Wang, Y. Wang, L. Sun, and Z. Han, “Banking human umbilical cord-derived mesenchymal stromal cells for clinical use,” Cell Transplant. 21(1), 207–216 (2012).
[Crossref] [PubMed]

H. Bai, P. Chen, H. Fang, L. Lin, G. Q. Tang, G. G. Mu, W. Gong, Z. P. Liu, H. Wu, H. Zhao, and Z. C. Han, “Detecting viability transitions of umbilical cord mesenchymal stem cells by Raman micro-spectroscopy,” Laser Phys. Lett. 8(1), 78–84 (2011).
[Crossref]

H. Bai, P. Chen, G. Q. Tang, L. Lin, H. Fang, G. G. Mu, W. Gong, Z. P. Liu, Z. B. Han, H. Zhao, and Z. C. Han, “Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability,” Laser Phys. 21(6), 1122–1129 (2011).
[Crossref]

Zhao, X.

H. Cheng, L. Qiu, J. Ma, H. Zhang, M. Cheng, W. Li, X. Zhao, and K. Liu, “Replicative senescence of human bone marrow and umbilical cord derived mesenchymal stem cells and their differentiation to adipocytes and osteoblasts,” Mol. Biol. Rep. 38(8), 5161–5168 (2011).
[Crossref] [PubMed]

Zhao, Y.

H. Hao, G. Chen, J. Liu, D. Ti, Y. Zhao, S. Xu, X. Fu, and W. Han, “Culturing on Wharton’s jelly extract delays mesenchymal stem cell senescence through p53 and p16INK4a/pRb pathways,” PLoS One 8(3), e58314 (2013).
[Crossref] [PubMed]

J. Ren, D. F. Stroncek, Y. Zhao, P. Jin, L. Castiello, S. Civini, H. Wang, J. Feng, K. Tran, S. A. Kuznetsov, P. G. Robey, and M. Sabatino, “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence,” Stem Cell Res. (Amst.) 11(3), 1060–1073 (2013).
[Crossref] [PubMed]

Zhong, J.

W. Gong, Z. Han, H. Zhao, Y. Wang, J. Wang, J. Zhong, B. Wang, S. Wang, Y. Wang, L. Sun, and Z. Han, “Banking human umbilical cord-derived mesenchymal stromal cells for clinical use,” Cell Transplant. 21(1), 207–216 (2012).
[Crossref] [PubMed]

Zwerschke, W.

M. C. Mitterberger, S. Lechner, M. Mattesich, and W. Zwerschke, “Adipogenic differentiation is impaired in replicative senescent human subcutaneous adipose-derived stromal/progenitor cells,” J. Gerontol. A Biol. Sci. Med. Sci. 69(1), 13–24 (2014).
[Crossref] [PubMed]

Ageing Res. Rev. (1)

S. Sethe, A. Scutt, and A. Stolzing, “Aging of mesenchymal stem cells,” Ageing Res. Rev. 5(1), 91–116 (2006).
[Crossref] [PubMed]

Anal. Chem. (4)

S. O. Konorov, H. G. Schulze, J. M. Piret, M. W. Blades, and R. F. Turner, “Label-free determination of the cell cycle phase in human embryonic stem cells by Raman microspectroscopy,” Anal. Chem. 85(19), 8996–9002 (2013).
[Crossref] [PubMed]

S. O. Konorov, H. G. Schulze, C. G. Atkins, J. M. Piret, S. A. Aparicio, R. F. B. Turner, and M. W. Blades, “Absolute quantification of intracellular glycogen content in human embryonic stem cells with Raman microspectroscopy,” Anal. Chem. 83(16), 6254–6258 (2011).
[Crossref] [PubMed]

J. W. Chan, D. K. Lieu, T. Huser, and R. A. Li, “Label-free separation of human embryonic stem cells and their cardiac derivatives using Raman spectroscopy,” Anal. Chem. 81(4), 1324–1331 (2009).
[Crossref] [PubMed]

H. G. Schulze, S. O. Konorov, N. J. Caron, J. M. Piret, M. W. Blades, and R. F. B. Turner, “Assessing differentiation status of human embryonic stem cells noninvasively using Raman microspectroscopy,” Anal. Chem. 82(12), 5020–5027 (2010).
[Crossref] [PubMed]

Analyst (Lond.) (1)

L. L. McManus, G. A. Burke, M. M. McCafferty, P. O’Hare, M. Modreanu, A. R. Boyd, and B. J. Meenan, “Raman spectroscopic monitoring of the osteogenic differentiation of human mesenchymal stem cells,” Analyst (Lond.) 136(12), 2471–2481 (2011).
[Crossref] [PubMed]

Biochim. Biophys. Acta (1)

F. C. Pascut, S. Kalra, V. George, N. Welch, C. Denning, and I. Notingher, “Non-invasive label-free monitoring the cardiac differentiation of human embryonic stem cells in-vitro by Raman spectroscopy,” Biochim. Biophys. Acta 1830(6), 3517–3524 (2013).
[Crossref] [PubMed]

Biopolymers (1)

I. Notingher, S. Verrier, S. Haque, J. M. Polak, and L. L. Hench, “Spectroscopic study of human lung epithelial cells (A549) in culture: living cells versus dead cells,” Biopolymers 72(4), 230–240 (2003).
[Crossref] [PubMed]

Cancer (1)

H. Motaln, C. Schichor, and T. T. Lah, “Human mesenchymal stem cells and their use in cell-based therapies,” Cancer 116(11), 2519–2530 (2010).
[Crossref] [PubMed]

Cell Death Dis. (1)

J. C. Estrada, Y. Torres, A. Benguría, A. Dopazo, E. Roche, L. Carrera-Quintanar, R. A. Pérez, J. A. Enríquez, R. Torres, J. C. Ramírez, E. Samper, and A. Bernad, “Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy,” Cell Death Dis. 4(6), e691 (2013).
[Crossref] [PubMed]

Cell Transplant. (1)

W. Gong, Z. Han, H. Zhao, Y. Wang, J. Wang, J. Zhong, B. Wang, S. Wang, Y. Wang, L. Sun, and Z. Han, “Banking human umbilical cord-derived mesenchymal stromal cells for clinical use,” Cell Transplant. 21(1), 207–216 (2012).
[Crossref] [PubMed]

Genes Genomics (1)

H. B. Noh, H. J. Ahn, W. J. Lee, K. B. Kwack, and Y. D. Kwon, “The molecular signature of in vitro senescence in human mesenchymal stem cells,” Genes Genomics 32(1), 87–93 (2010).
[Crossref]

J. Biomed. Opt. (1)

F. C. Pascut, H. T. Goh, V. George, C. Denning, and I. Notingher, “Toward label-free Raman-activated cell sorting of cardiomyocytes derived from human embryonic stem cells,” J. Biomed. Opt. 16(4), 045002 (2011).
[Crossref] [PubMed]

J. Gerontol. A Biol. Sci. Med. Sci. (1)

M. C. Mitterberger, S. Lechner, M. Mattesich, and W. Zwerschke, “Adipogenic differentiation is impaired in replicative senescent human subcutaneous adipose-derived stromal/progenitor cells,” J. Gerontol. A Biol. Sci. Med. Sci. 69(1), 13–24 (2014).
[Crossref] [PubMed]

J. Mater. Sci. Mater. Med. (1)

A. R. Boyd, G. A. Burke, and B. J. Meenan, “Monitoring cellular behaviour using Raman spectroscopy for tissue engineering and regenerative medicine applications,” J. Mater. Sci. Mater. Med. 21(8), 2317–2324 (2010).
[Crossref] [PubMed]

J. Raman Spectrosc. (1)

H. K. Chiang, F. Y. Peng, S. C. Hung, and Y. C. Feng, “In situ Raman spectroscopic monitoring of hydroxyapatite as human mesenchymal stem cells differentiate into osteoblasts,” J. Raman Spectrosc. 40(5), 546–549 (2009).
[Crossref]

Laser Phys. (1)

H. Bai, P. Chen, G. Q. Tang, L. Lin, H. Fang, G. G. Mu, W. Gong, Z. P. Liu, Z. B. Han, H. Zhao, and Z. C. Han, “Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability,” Laser Phys. 21(6), 1122–1129 (2011).
[Crossref]

Laser Phys. Lett. (1)

H. Bai, P. Chen, H. Fang, L. Lin, G. Q. Tang, G. G. Mu, W. Gong, Z. P. Liu, H. Wu, H. Zhao, and Z. C. Han, “Detecting viability transitions of umbilical cord mesenchymal stem cells by Raman micro-spectroscopy,” Laser Phys. Lett. 8(1), 78–84 (2011).
[Crossref]

Mech. Ageing Dev. (1)

J. S. Park, H. Y. Kim, H. W. Kim, G. N. Chae, H. T. Oh, J. Y. Park, H. Shim, M. Seo, E. Y. Shin, E. G. Kim, S. C. Park, and S. J. Kwak, “Increased caveolin-1, a cause for the declined adipogenic potential of senescent human mesenchymal stem cells,” Mech. Ageing Dev. 126(5), 551–559 (2005).
[Crossref] [PubMed]

Med. Laser Appl. (1)

M. Pudlas, D. A. C. Berrio, M. Votteler, S. Koch, S. Thude, H. Walles, and K. Schenke-Layland, “Non-contact discrimination of human bone marrow-derived mesenchymal stem cells and fibroblasts using Raman spectroscopy,” Med. Laser Appl. 26(3), 119–125 (2011).
[Crossref]

Mol. Biol. Cell (1)

M. Terai, T. Uyama, T. Sugiki, X. K. Li, A. Umezawa, and T. Kiyono, “Immortalization of human fetal cells: the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway,” Mol. Biol. Cell 16(3), 1491–1499 (2005).
[Crossref] [PubMed]

Mol. Biol. Rep. (1)

H. Cheng, L. Qiu, J. Ma, H. Zhang, M. Cheng, W. Li, X. Zhao, and K. Liu, “Replicative senescence of human bone marrow and umbilical cord derived mesenchymal stem cells and their differentiation to adipocytes and osteoblasts,” Mol. Biol. Rep. 38(8), 5161–5168 (2011).
[Crossref] [PubMed]

PLoS One (2)

H. Hao, G. Chen, J. Liu, D. Ti, Y. Zhao, S. Xu, X. Fu, and W. Han, “Culturing on Wharton’s jelly extract delays mesenchymal stem cell senescence through p53 and p16INK4a/pRb pathways,” PLoS One 8(3), e58314 (2013).
[Crossref] [PubMed]

W. Wagner, P. Horn, M. Castoldi, A. Diehlmann, S. Bork, R. Saffrich, V. Benes, J. Blake, S. Pfister, V. Eckstein, and A. D. Ho, “Replicative senescence of mesenchymal stem cells: a continuous and organized process,” PLoS One 3(5), e2213 (2008).
[Crossref] [PubMed]

Stem Cell Res. (Amst.) (1)

J. Ren, D. F. Stroncek, Y. Zhao, P. Jin, L. Castiello, S. Civini, H. Wang, J. Feng, K. Tran, S. A. Kuznetsov, P. G. Robey, and M. Sabatino, “Intra-subject variability in human bone marrow stromal cell (BMSC) replicative senescence: molecular changes associated with BMSC senescence,” Stem Cell Res. (Amst.) 11(3), 1060–1073 (2013).
[Crossref] [PubMed]

Stem Cells (4)

J. C. Sepúlveda, M. Tomé, M. E. Fernández, M. Delgado, J. Campisi, A. Bernad, and M. A. González, “Cell senescence abrogates the therapeutic potential of human mesenchymal stem cells in the lethal endotoxemia model,” Stem Cells 32(7), 1865–1877 (2014).
[Crossref] [PubMed]

D. Baksh, R. Yao, and R. S. Tuan, “Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow,” Stem Cells 25(6), 1384–1392 (2007).
[Crossref] [PubMed]

K. E. Mitchell, M. L. Weiss, B. M. Mitchell, P. Martin, D. Davis, L. Morales, B. Helwig, M. Beerenstrauch, K. Abou-Easa, T. Hildreth, D. Troyer, and S. Medicetty, “Matrix cells from Wharton’s jelly form neurons and glia,” Stem Cells 21(1), 50–60 (2003).
[Crossref] [PubMed]

H. S. Wang, S. C. Hung, S. T. Peng, C. C. Huang, H. M. Wei, Y. J. Guo, Y. S. Fu, M. C. Lai, and C. C. Chen, “Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord,” Stem Cells 22(7), 1330–1337 (2004).
[Crossref] [PubMed]

Stem Cells Dev. (1)

L. Harkness, S. M. Novikov, J. Beermann, S. I. Bozhevolnyi, and M. Kassem, “Identification of abnormal stem cells using Raman spectroscopy,” Stem Cells Dev. 21(12), 2152–2159 (2012).
[Crossref] [PubMed]

Vib. Spectrosc. (1)

I. Notingher, I. Bisson, J. M. Polak, and L. L. Hench, “In situ spectroscopic study of nucleic acids in differentiating embryonic stem cells,” Vib. Spectrosc. 35(1-2), 199–203 (2004).
[Crossref]

Other (1)

Y. M. Xu, Raman spectroscopy in application of structure biology (Chemical Industry Press, 2005).

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Figures (5)

Fig. 1
Fig. 1 Average Raman spectra from senescent hUC-MSCs in P30 (a) and from young cells in P5 (b) and their difference spectrum (c = a-b). Spectra are shifted vertically for clarity. Gray bars highlight the spectral differences.
Fig. 2
Fig. 2 Comparison of relative intensities of Raman peaks between young (P5) and aging (P30) hUC-MSCs. * means p<0.05 obtained by Student’s t-test.
Fig. 3
Fig. 3 Average Raman spectra from hUC-MSCs in P5 (a), P10 (b), P20 (c) and P30 (d). Spectra are shifted vertically for clarity. The gray bar highlights the spectral changes with cell senescence.
Fig. 4
Fig. 4 (A) Change of I1157 with cell senescence. (B) Change of I1157 / I1174 with cell senescence. Values p1, p2, p3 were obtained by the Student’s t-test analysis between cells in P5 and P10, P10 and P20, P20 and P30 respectively.
Fig. 5
Fig. 5 (A) Average autofluorescence spectra from aging hUC-MSCs (P20) and young cells (P5). (B) Comparison of the fluorescent peak height at 336nm. Excitation wavelength at 280nm. p value was obtained by Student’s t-test.

Tables (1)

Tables Icon

Table 1 Raman peak assignment

Metrics