Abstract

Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capable of operating Light Sheet Microscopes for automatized 3D high-throughput imaging on three-dimensional cell cultures and model organisms like zebrafish, oriented to massive drug screening.

© 2015 Optical Society of America

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  3. B. Pruvot, A. Jacquel, N. Droin, P. Auberger, D. Bouscary, J. Tamburini, M. Muller, M. Fontenay, J. Chluba, and E. Solary, “Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy,” Haematologica 96(4), 612–616 (2011).
    [Crossref] [PubMed]
  4. J. Friedrich, C. Seidel, R. Ebner, and L. A. Kunz-Schughart, “Spheroid-based drug screen: considerations and practical approach,” Nat. Protoc. 4(3), 309–324 (2009).
    [Crossref] [PubMed]
  5. F. Pampaloni, E. G. Reynaud, and E. H. K. Stelzer, “The third dimension bridges the gap between cell culture and live tissue,” Nat. Rev. Mol. Cell Biol. 8(10), 839–845 (2007).
    [Crossref] [PubMed]
  6. F. Pampaloni, B. J. Chang, and E. H. K. Stelzer, “Light sheet-based fluorescence microscopy (LSFM) for the quantitative imaging of cells and tissues,” Cell Tissue Res. 360(1), 129–141 (2015).
    [Crossref] [PubMed]
  7. C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010).
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  8. S. H. Cho, J. M. Godin, C. Chen, W. Qiao, H. Lee, and Y. Lo, “Recent advancements in optofluidicflow cytometer,” Biomicrofluidics 4(4), 043001 (2010).
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  9. J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
    [Crossref] [PubMed]
  10. P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4(4), 311–313 (2007).
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    [Crossref] [PubMed]
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    [Crossref] [PubMed]
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    [Crossref]

2015 (3)

F. Pampaloni, B. J. Chang, and E. H. K. Stelzer, “Light sheet-based fluorescence microscopy (LSFM) for the quantitative imaging of cells and tissues,” Cell Tissue Res. 360(1), 129–141 (2015).
[Crossref] [PubMed]

R. Galland, G. Grenci, A. Aravind, V. Viasnoff, V. Studer, and J. B. Sibarita, “3D high- and super-resolution imaging using single-objective SPIM,” Nat. Methods 12(7), 641–644 (2015).
[Crossref] [PubMed]

O. E. Olarte, J. Andilla, D. Artigas, and P. Loza-Alvarez, “Decoupled illumination detection in light sheet microscopy for fast volumetric imaging,” Optica 2(8), 702–705 (2015).
[Crossref]

2014 (6)

A. Kumar, Y. Wu, R. Christensen, P. Chandris, W. Gandler, E. McCreedy, A. Bokinsky, D. A. Colón-Ramos, Z. Bao, M. McAuliffe, G. Rondeau, and H. Shroff, “Dual-view plane illumination microscopy for rapid and spatially isotropic imaging,” Nat. Protoc. 9(11), 2555–2573 (2014).
[Crossref] [PubMed]

R. Regmi, K. Mohan, and P. P. Mondal, “High resolution light-sheet based high-throughput imaging cytometry system enables visualization of intra-cellular organelles,” AIP Adv. 4(9), 097125 (2014).
[Crossref]

E. J. Gualda, D. Simão, C. Pinto, P. M. Alves, and C. Brito, “Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy,” Front. Cell. Neurosci. 8, 221 (2014).
[Crossref] [PubMed]

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref] [PubMed]

S. P. Rebelo, R. Costa, M. Estrada, V. Shevchenko, C. Brito, and P. M. Alves, “HepaRG microencapsulated spheroids in DMSO-free culture: novel culturing approaches for enhanced xenobiotic and biosynthetic metabolism,” Arch. Toxicol. 89, 1347–1358 (2014).
[PubMed]

E. G. Reynaud, J. Peychl, J. Huisken, and P. Tomancak, “Guide to light-sheet microscopy for adventurous biologists,” Nat. Methods 12(1), 30–34 (2014).
[Crossref] [PubMed]

2013 (3)

M. B. Ahrens, M. B. Orger, D. N. Robson, J. M. Li, and P. J. Keller, “Whole-brain functional imaging at cellular resolution using light-sheet microscopy,” Nat. Methods 10(5), 413–420 (2013).
[Crossref] [PubMed]

E. J. Gualda, T. Vale, P. Almada, J. A. Feijó, G. G. Martins, and N. Moreno, “OpenSpinMicroscopy: an open-source integrated microscopy platform,” Nat. Methods 10(7), 599–600 (2013).
[Crossref] [PubMed]

J. Wu, J. Li, and R. K. Y. Chan, “A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis,” Opt. Express 21(12), 14474–14480 (2013).
[Crossref] [PubMed]

2012 (4)

P. Neuži, S. Giselbrecht, K. Länge, T. J. Huang, and A. Manz, “Revisiting lab-on-a-chip technology for drug discovery,” Nat. Rev. Drug Discov. 11(8), 620–632 (2012).
[Crossref] [PubMed]

A. Kaufmann, M. Mickoleit, M. Weber, and J. Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139(17), 3242–3247 (2012).
[Crossref] [PubMed]

T. Bruns, S. Schickinger, R. Wittig, and H. Schneckenburger, “Preparation strategy and illumination of three-dimensional cell cultures in light sheet-based fluorescence microscopy,” J. Biomed. Opt. 17(10), 1015181 (2012).
[Crossref] [PubMed]

J. R. Mathias, M. T. Saxena, and J. S. Mumm, “Advances in zebrafish chemical screening technologies,” Future Med. Chem. 4(14), 1811–1822 (2012).
[Crossref] [PubMed]

2011 (2)

B. Pruvot, A. Jacquel, N. Droin, P. Auberger, D. Bouscary, J. Tamburini, M. Muller, M. Fontenay, J. Chluba, and E. Solary, “Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy,” Haematologica 96(4), 612–616 (2011).
[Crossref] [PubMed]

C. A. Lessman, “The developing zebrafish (danio rerio): a vertebrate model for high-throughput screening of chemical libraries,” Birth Defects Res. C Embryo Today 93(3), 268–280 (2011).
[Crossref] [PubMed]

2010 (2)

C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010).
[Crossref] [PubMed]

S. H. Cho, J. M. Godin, C. Chen, W. Qiao, H. Lee, and Y. Lo, “Recent advancements in optofluidicflow cytometer,” Biomicrofluidics 4(4), 043001 (2010).
[Crossref]

2009 (1)

J. Friedrich, C. Seidel, R. Ebner, and L. A. Kunz-Schughart, “Spheroid-based drug screen: considerations and practical approach,” Nat. Protoc. 4(3), 309–324 (2009).
[Crossref] [PubMed]

2007 (2)

F. Pampaloni, E. G. Reynaud, and E. H. K. Stelzer, “The third dimension bridges the gap between cell culture and live tissue,” Nat. Rev. Mol. Cell Biol. 8(10), 839–845 (2007).
[Crossref] [PubMed]

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4(4), 311–313 (2007).
[PubMed]

2004 (1)

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Ahrens, M. B.

M. B. Ahrens, M. B. Orger, D. N. Robson, J. M. Li, and P. J. Keller, “Whole-brain functional imaging at cellular resolution using light-sheet microscopy,” Nat. Methods 10(5), 413–420 (2013).
[Crossref] [PubMed]

Almada, P.

E. J. Gualda, T. Vale, P. Almada, J. A. Feijó, G. G. Martins, and N. Moreno, “OpenSpinMicroscopy: an open-source integrated microscopy platform,” Nat. Methods 10(7), 599–600 (2013).
[Crossref] [PubMed]

Alves, P. M.

E. J. Gualda, D. Simão, C. Pinto, P. M. Alves, and C. Brito, “Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy,” Front. Cell. Neurosci. 8, 221 (2014).
[Crossref] [PubMed]

S. P. Rebelo, R. Costa, M. Estrada, V. Shevchenko, C. Brito, and P. M. Alves, “HepaRG microencapsulated spheroids in DMSO-free culture: novel culturing approaches for enhanced xenobiotic and biosynthetic metabolism,” Arch. Toxicol. 89, 1347–1358 (2014).
[PubMed]

Amodaj, N.

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref] [PubMed]

Andilla, J.

Aravind, A.

R. Galland, G. Grenci, A. Aravind, V. Viasnoff, V. Studer, and J. B. Sibarita, “3D high- and super-resolution imaging using single-objective SPIM,” Nat. Methods 12(7), 641–644 (2015).
[Crossref] [PubMed]

Artigas, D.

Auberger, P.

B. Pruvot, A. Jacquel, N. Droin, P. Auberger, D. Bouscary, J. Tamburini, M. Muller, M. Fontenay, J. Chluba, and E. Solary, “Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy,” Haematologica 96(4), 612–616 (2011).
[Crossref] [PubMed]

Bao, Z.

A. Kumar, Y. Wu, R. Christensen, P. Chandris, W. Gandler, E. McCreedy, A. Bokinsky, D. A. Colón-Ramos, Z. Bao, M. McAuliffe, G. Rondeau, and H. Shroff, “Dual-view plane illumination microscopy for rapid and spatially isotropic imaging,” Nat. Protoc. 9(11), 2555–2573 (2014).
[Crossref] [PubMed]

Bokinsky, A.

A. Kumar, Y. Wu, R. Christensen, P. Chandris, W. Gandler, E. McCreedy, A. Bokinsky, D. A. Colón-Ramos, Z. Bao, M. McAuliffe, G. Rondeau, and H. Shroff, “Dual-view plane illumination microscopy for rapid and spatially isotropic imaging,” Nat. Protoc. 9(11), 2555–2573 (2014).
[Crossref] [PubMed]

Bouscary, D.

B. Pruvot, A. Jacquel, N. Droin, P. Auberger, D. Bouscary, J. Tamburini, M. Muller, M. Fontenay, J. Chluba, and E. Solary, “Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy,” Haematologica 96(4), 612–616 (2011).
[Crossref] [PubMed]

Brito, C.

E. J. Gualda, D. Simão, C. Pinto, P. M. Alves, and C. Brito, “Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy,” Front. Cell. Neurosci. 8, 221 (2014).
[Crossref] [PubMed]

S. P. Rebelo, R. Costa, M. Estrada, V. Shevchenko, C. Brito, and P. M. Alves, “HepaRG microencapsulated spheroids in DMSO-free culture: novel culturing approaches for enhanced xenobiotic and biosynthetic metabolism,” Arch. Toxicol. 89, 1347–1358 (2014).
[PubMed]

Bruns, T.

T. Bruns, S. Schickinger, R. Wittig, and H. Schneckenburger, “Preparation strategy and illumination of three-dimensional cell cultures in light sheet-based fluorescence microscopy,” J. Biomed. Opt. 17(10), 1015181 (2012).
[Crossref] [PubMed]

Chan, R. K. Y.

Chandris, P.

A. Kumar, Y. Wu, R. Christensen, P. Chandris, W. Gandler, E. McCreedy, A. Bokinsky, D. A. Colón-Ramos, Z. Bao, M. McAuliffe, G. Rondeau, and H. Shroff, “Dual-view plane illumination microscopy for rapid and spatially isotropic imaging,” Nat. Protoc. 9(11), 2555–2573 (2014).
[Crossref] [PubMed]

Chang, B. J.

F. Pampaloni, B. J. Chang, and E. H. K. Stelzer, “Light sheet-based fluorescence microscopy (LSFM) for the quantitative imaging of cells and tissues,” Cell Tissue Res. 360(1), 129–141 (2015).
[Crossref] [PubMed]

Chang, T. Y.

C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010).
[Crossref] [PubMed]

Chen, C.

S. H. Cho, J. M. Godin, C. Chen, W. Qiao, H. Lee, and Y. Lo, “Recent advancements in optofluidicflow cytometer,” Biomicrofluidics 4(4), 043001 (2010).
[Crossref]

Chluba, J.

B. Pruvot, A. Jacquel, N. Droin, P. Auberger, D. Bouscary, J. Tamburini, M. Muller, M. Fontenay, J. Chluba, and E. Solary, “Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy,” Haematologica 96(4), 612–616 (2011).
[Crossref] [PubMed]

Cho, S. H.

S. H. Cho, J. M. Godin, C. Chen, W. Qiao, H. Lee, and Y. Lo, “Recent advancements in optofluidicflow cytometer,” Biomicrofluidics 4(4), 043001 (2010).
[Crossref]

Christensen, R.

A. Kumar, Y. Wu, R. Christensen, P. Chandris, W. Gandler, E. McCreedy, A. Bokinsky, D. A. Colón-Ramos, Z. Bao, M. McAuliffe, G. Rondeau, and H. Shroff, “Dual-view plane illumination microscopy for rapid and spatially isotropic imaging,” Nat. Protoc. 9(11), 2555–2573 (2014).
[Crossref] [PubMed]

Colón-Ramos, D. A.

A. Kumar, Y. Wu, R. Christensen, P. Chandris, W. Gandler, E. McCreedy, A. Bokinsky, D. A. Colón-Ramos, Z. Bao, M. McAuliffe, G. Rondeau, and H. Shroff, “Dual-view plane illumination microscopy for rapid and spatially isotropic imaging,” Nat. Protoc. 9(11), 2555–2573 (2014).
[Crossref] [PubMed]

Costa, R.

S. P. Rebelo, R. Costa, M. Estrada, V. Shevchenko, C. Brito, and P. M. Alves, “HepaRG microencapsulated spheroids in DMSO-free culture: novel culturing approaches for enhanced xenobiotic and biosynthetic metabolism,” Arch. Toxicol. 89, 1347–1358 (2014).
[PubMed]

Del Bene, F.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Droin, N.

B. Pruvot, A. Jacquel, N. Droin, P. Auberger, D. Bouscary, J. Tamburini, M. Muller, M. Fontenay, J. Chluba, and E. Solary, “Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy,” Haematologica 96(4), 612–616 (2011).
[Crossref] [PubMed]

Ebner, R.

J. Friedrich, C. Seidel, R. Ebner, and L. A. Kunz-Schughart, “Spheroid-based drug screen: considerations and practical approach,” Nat. Protoc. 4(3), 309–324 (2009).
[Crossref] [PubMed]

Edelstein, A. D.

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref] [PubMed]

Estrada, M.

S. P. Rebelo, R. Costa, M. Estrada, V. Shevchenko, C. Brito, and P. M. Alves, “HepaRG microencapsulated spheroids in DMSO-free culture: novel culturing approaches for enhanced xenobiotic and biosynthetic metabolism,” Arch. Toxicol. 89, 1347–1358 (2014).
[PubMed]

Feijó, J. A.

E. J. Gualda, T. Vale, P. Almada, J. A. Feijó, G. G. Martins, and N. Moreno, “OpenSpinMicroscopy: an open-source integrated microscopy platform,” Nat. Methods 10(7), 599–600 (2013).
[Crossref] [PubMed]

Fontenay, M.

B. Pruvot, A. Jacquel, N. Droin, P. Auberger, D. Bouscary, J. Tamburini, M. Muller, M. Fontenay, J. Chluba, and E. Solary, “Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy,” Haematologica 96(4), 612–616 (2011).
[Crossref] [PubMed]

Friedrich, J.

J. Friedrich, C. Seidel, R. Ebner, and L. A. Kunz-Schughart, “Spheroid-based drug screen: considerations and practical approach,” Nat. Protoc. 4(3), 309–324 (2009).
[Crossref] [PubMed]

Galland, R.

R. Galland, G. Grenci, A. Aravind, V. Viasnoff, V. Studer, and J. B. Sibarita, “3D high- and super-resolution imaging using single-objective SPIM,” Nat. Methods 12(7), 641–644 (2015).
[Crossref] [PubMed]

Gandler, W.

A. Kumar, Y. Wu, R. Christensen, P. Chandris, W. Gandler, E. McCreedy, A. Bokinsky, D. A. Colón-Ramos, Z. Bao, M. McAuliffe, G. Rondeau, and H. Shroff, “Dual-view plane illumination microscopy for rapid and spatially isotropic imaging,” Nat. Protoc. 9(11), 2555–2573 (2014).
[Crossref] [PubMed]

Gilleland, C. L.

C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010).
[Crossref] [PubMed]

Giselbrecht, S.

P. Neuži, S. Giselbrecht, K. Länge, T. J. Huang, and A. Manz, “Revisiting lab-on-a-chip technology for drug discovery,” Nat. Rev. Drug Discov. 11(8), 620–632 (2012).
[Crossref] [PubMed]

Godin, J. M.

S. H. Cho, J. M. Godin, C. Chen, W. Qiao, H. Lee, and Y. Lo, “Recent advancements in optofluidicflow cytometer,” Biomicrofluidics 4(4), 043001 (2010).
[Crossref]

Greger, K.

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4(4), 311–313 (2007).
[PubMed]

Grenci, G.

R. Galland, G. Grenci, A. Aravind, V. Viasnoff, V. Studer, and J. B. Sibarita, “3D high- and super-resolution imaging using single-objective SPIM,” Nat. Methods 12(7), 641–644 (2015).
[Crossref] [PubMed]

Gualda, E. J.

E. J. Gualda, D. Simão, C. Pinto, P. M. Alves, and C. Brito, “Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy,” Front. Cell. Neurosci. 8, 221 (2014).
[Crossref] [PubMed]

E. J. Gualda, T. Vale, P. Almada, J. A. Feijó, G. G. Martins, and N. Moreno, “OpenSpinMicroscopy: an open-source integrated microscopy platform,” Nat. Methods 10(7), 599–600 (2013).
[Crossref] [PubMed]

Huang, T. J.

P. Neuži, S. Giselbrecht, K. Länge, T. J. Huang, and A. Manz, “Revisiting lab-on-a-chip technology for drug discovery,” Nat. Rev. Drug Discov. 11(8), 620–632 (2012).
[Crossref] [PubMed]

Huisken, J.

E. G. Reynaud, J. Peychl, J. Huisken, and P. Tomancak, “Guide to light-sheet microscopy for adventurous biologists,” Nat. Methods 12(1), 30–34 (2014).
[Crossref] [PubMed]

A. Kaufmann, M. Mickoleit, M. Weber, and J. Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139(17), 3242–3247 (2012).
[Crossref] [PubMed]

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Jacquel, A.

B. Pruvot, A. Jacquel, N. Droin, P. Auberger, D. Bouscary, J. Tamburini, M. Muller, M. Fontenay, J. Chluba, and E. Solary, “Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy,” Haematologica 96(4), 612–616 (2011).
[Crossref] [PubMed]

Kaufmann, A.

A. Kaufmann, M. Mickoleit, M. Weber, and J. Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139(17), 3242–3247 (2012).
[Crossref] [PubMed]

Keller, P. J.

M. B. Ahrens, M. B. Orger, D. N. Robson, J. M. Li, and P. J. Keller, “Whole-brain functional imaging at cellular resolution using light-sheet microscopy,” Nat. Methods 10(5), 413–420 (2013).
[Crossref] [PubMed]

Koo, B. K.

C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010).
[Crossref] [PubMed]

Kumar, A.

A. Kumar, Y. Wu, R. Christensen, P. Chandris, W. Gandler, E. McCreedy, A. Bokinsky, D. A. Colón-Ramos, Z. Bao, M. McAuliffe, G. Rondeau, and H. Shroff, “Dual-view plane illumination microscopy for rapid and spatially isotropic imaging,” Nat. Protoc. 9(11), 2555–2573 (2014).
[Crossref] [PubMed]

Kunz-Schughart, L. A.

J. Friedrich, C. Seidel, R. Ebner, and L. A. Kunz-Schughart, “Spheroid-based drug screen: considerations and practical approach,” Nat. Protoc. 4(3), 309–324 (2009).
[Crossref] [PubMed]

Länge, K.

P. Neuži, S. Giselbrecht, K. Länge, T. J. Huang, and A. Manz, “Revisiting lab-on-a-chip technology for drug discovery,” Nat. Rev. Drug Discov. 11(8), 620–632 (2012).
[Crossref] [PubMed]

Lee, H.

S. H. Cho, J. M. Godin, C. Chen, W. Qiao, H. Lee, and Y. Lo, “Recent advancements in optofluidicflow cytometer,” Biomicrofluidics 4(4), 043001 (2010).
[Crossref]

Lessman, C. A.

C. A. Lessman, “The developing zebrafish (danio rerio): a vertebrate model for high-throughput screening of chemical libraries,” Birth Defects Res. C Embryo Today 93(3), 268–280 (2011).
[Crossref] [PubMed]

Li, J.

Li, J. M.

M. B. Ahrens, M. B. Orger, D. N. Robson, J. M. Li, and P. J. Keller, “Whole-brain functional imaging at cellular resolution using light-sheet microscopy,” Nat. Methods 10(5), 413–420 (2013).
[Crossref] [PubMed]

Lo, Y.

S. H. Cho, J. M. Godin, C. Chen, W. Qiao, H. Lee, and Y. Lo, “Recent advancements in optofluidicflow cytometer,” Biomicrofluidics 4(4), 043001 (2010).
[Crossref]

Loza-Alvarez, P.

Manz, A.

P. Neuži, S. Giselbrecht, K. Länge, T. J. Huang, and A. Manz, “Revisiting lab-on-a-chip technology for drug discovery,” Nat. Rev. Drug Discov. 11(8), 620–632 (2012).
[Crossref] [PubMed]

Marcello, M.

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4(4), 311–313 (2007).
[PubMed]

Martins, G. G.

E. J. Gualda, T. Vale, P. Almada, J. A. Feijó, G. G. Martins, and N. Moreno, “OpenSpinMicroscopy: an open-source integrated microscopy platform,” Nat. Methods 10(7), 599–600 (2013).
[Crossref] [PubMed]

Mathias, J. R.

J. R. Mathias, M. T. Saxena, and J. S. Mumm, “Advances in zebrafish chemical screening technologies,” Future Med. Chem. 4(14), 1811–1822 (2012).
[Crossref] [PubMed]

McAuliffe, M.

A. Kumar, Y. Wu, R. Christensen, P. Chandris, W. Gandler, E. McCreedy, A. Bokinsky, D. A. Colón-Ramos, Z. Bao, M. McAuliffe, G. Rondeau, and H. Shroff, “Dual-view plane illumination microscopy for rapid and spatially isotropic imaging,” Nat. Protoc. 9(11), 2555–2573 (2014).
[Crossref] [PubMed]

McCreedy, E.

A. Kumar, Y. Wu, R. Christensen, P. Chandris, W. Gandler, E. McCreedy, A. Bokinsky, D. A. Colón-Ramos, Z. Bao, M. McAuliffe, G. Rondeau, and H. Shroff, “Dual-view plane illumination microscopy for rapid and spatially isotropic imaging,” Nat. Protoc. 9(11), 2555–2573 (2014).
[Crossref] [PubMed]

Mickoleit, M.

A. Kaufmann, M. Mickoleit, M. Weber, and J. Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139(17), 3242–3247 (2012).
[Crossref] [PubMed]

Mohan, K.

R. Regmi, K. Mohan, and P. P. Mondal, “High resolution light-sheet based high-throughput imaging cytometry system enables visualization of intra-cellular organelles,” AIP Adv. 4(9), 097125 (2014).
[Crossref]

Mondal, P. P.

R. Regmi, K. Mohan, and P. P. Mondal, “High resolution light-sheet based high-throughput imaging cytometry system enables visualization of intra-cellular organelles,” AIP Adv. 4(9), 097125 (2014).
[Crossref]

Moreno, N.

E. J. Gualda, T. Vale, P. Almada, J. A. Feijó, G. G. Martins, and N. Moreno, “OpenSpinMicroscopy: an open-source integrated microscopy platform,” Nat. Methods 10(7), 599–600 (2013).
[Crossref] [PubMed]

Muller, M.

B. Pruvot, A. Jacquel, N. Droin, P. Auberger, D. Bouscary, J. Tamburini, M. Muller, M. Fontenay, J. Chluba, and E. Solary, “Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy,” Haematologica 96(4), 612–616 (2011).
[Crossref] [PubMed]

Mumm, J. S.

J. R. Mathias, M. T. Saxena, and J. S. Mumm, “Advances in zebrafish chemical screening technologies,” Future Med. Chem. 4(14), 1811–1822 (2012).
[Crossref] [PubMed]

Neuži, P.

P. Neuži, S. Giselbrecht, K. Länge, T. J. Huang, and A. Manz, “Revisiting lab-on-a-chip technology for drug discovery,” Nat. Rev. Drug Discov. 11(8), 620–632 (2012).
[Crossref] [PubMed]

Olarte, O. E.

Orger, M. B.

M. B. Ahrens, M. B. Orger, D. N. Robson, J. M. Li, and P. J. Keller, “Whole-brain functional imaging at cellular resolution using light-sheet microscopy,” Nat. Methods 10(5), 413–420 (2013).
[Crossref] [PubMed]

Pampaloni, F.

F. Pampaloni, B. J. Chang, and E. H. K. Stelzer, “Light sheet-based fluorescence microscopy (LSFM) for the quantitative imaging of cells and tissues,” Cell Tissue Res. 360(1), 129–141 (2015).
[Crossref] [PubMed]

F. Pampaloni, E. G. Reynaud, and E. H. K. Stelzer, “The third dimension bridges the gap between cell culture and live tissue,” Nat. Rev. Mol. Cell Biol. 8(10), 839–845 (2007).
[Crossref] [PubMed]

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4(4), 311–313 (2007).
[PubMed]

Pardo-Martin, C.

C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010).
[Crossref] [PubMed]

Peychl, J.

E. G. Reynaud, J. Peychl, J. Huisken, and P. Tomancak, “Guide to light-sheet microscopy for adventurous biologists,” Nat. Methods 12(1), 30–34 (2014).
[Crossref] [PubMed]

Pinkard, H.

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref] [PubMed]

Pinto, C.

E. J. Gualda, D. Simão, C. Pinto, P. M. Alves, and C. Brito, “Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy,” Front. Cell. Neurosci. 8, 221 (2014).
[Crossref] [PubMed]

Pruvot, B.

B. Pruvot, A. Jacquel, N. Droin, P. Auberger, D. Bouscary, J. Tamburini, M. Muller, M. Fontenay, J. Chluba, and E. Solary, “Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy,” Haematologica 96(4), 612–616 (2011).
[Crossref] [PubMed]

Qiao, W.

S. H. Cho, J. M. Godin, C. Chen, W. Qiao, H. Lee, and Y. Lo, “Recent advancements in optofluidicflow cytometer,” Biomicrofluidics 4(4), 043001 (2010).
[Crossref]

Rebelo, S. P.

S. P. Rebelo, R. Costa, M. Estrada, V. Shevchenko, C. Brito, and P. M. Alves, “HepaRG microencapsulated spheroids in DMSO-free culture: novel culturing approaches for enhanced xenobiotic and biosynthetic metabolism,” Arch. Toxicol. 89, 1347–1358 (2014).
[PubMed]

Regmi, R.

R. Regmi, K. Mohan, and P. P. Mondal, “High resolution light-sheet based high-throughput imaging cytometry system enables visualization of intra-cellular organelles,” AIP Adv. 4(9), 097125 (2014).
[Crossref]

Reynaud, E. G.

E. G. Reynaud, J. Peychl, J. Huisken, and P. Tomancak, “Guide to light-sheet microscopy for adventurous biologists,” Nat. Methods 12(1), 30–34 (2014).
[Crossref] [PubMed]

F. Pampaloni, E. G. Reynaud, and E. H. K. Stelzer, “The third dimension bridges the gap between cell culture and live tissue,” Nat. Rev. Mol. Cell Biol. 8(10), 839–845 (2007).
[Crossref] [PubMed]

Robson, D. N.

M. B. Ahrens, M. B. Orger, D. N. Robson, J. M. Li, and P. J. Keller, “Whole-brain functional imaging at cellular resolution using light-sheet microscopy,” Nat. Methods 10(5), 413–420 (2013).
[Crossref] [PubMed]

Rondeau, G.

A. Kumar, Y. Wu, R. Christensen, P. Chandris, W. Gandler, E. McCreedy, A. Bokinsky, D. A. Colón-Ramos, Z. Bao, M. McAuliffe, G. Rondeau, and H. Shroff, “Dual-view plane illumination microscopy for rapid and spatially isotropic imaging,” Nat. Protoc. 9(11), 2555–2573 (2014).
[Crossref] [PubMed]

Saxena, M. T.

J. R. Mathias, M. T. Saxena, and J. S. Mumm, “Advances in zebrafish chemical screening technologies,” Future Med. Chem. 4(14), 1811–1822 (2012).
[Crossref] [PubMed]

Schickinger, S.

T. Bruns, S. Schickinger, R. Wittig, and H. Schneckenburger, “Preparation strategy and illumination of three-dimensional cell cultures in light sheet-based fluorescence microscopy,” J. Biomed. Opt. 17(10), 1015181 (2012).
[Crossref] [PubMed]

Schneckenburger, H.

T. Bruns, S. Schickinger, R. Wittig, and H. Schneckenburger, “Preparation strategy and illumination of three-dimensional cell cultures in light sheet-based fluorescence microscopy,” J. Biomed. Opt. 17(10), 1015181 (2012).
[Crossref] [PubMed]

Seidel, C.

J. Friedrich, C. Seidel, R. Ebner, and L. A. Kunz-Schughart, “Spheroid-based drug screen: considerations and practical approach,” Nat. Protoc. 4(3), 309–324 (2009).
[Crossref] [PubMed]

Shevchenko, V.

S. P. Rebelo, R. Costa, M. Estrada, V. Shevchenko, C. Brito, and P. M. Alves, “HepaRG microencapsulated spheroids in DMSO-free culture: novel culturing approaches for enhanced xenobiotic and biosynthetic metabolism,” Arch. Toxicol. 89, 1347–1358 (2014).
[PubMed]

Shroff, H.

A. Kumar, Y. Wu, R. Christensen, P. Chandris, W. Gandler, E. McCreedy, A. Bokinsky, D. A. Colón-Ramos, Z. Bao, M. McAuliffe, G. Rondeau, and H. Shroff, “Dual-view plane illumination microscopy for rapid and spatially isotropic imaging,” Nat. Protoc. 9(11), 2555–2573 (2014).
[Crossref] [PubMed]

Sibarita, J. B.

R. Galland, G. Grenci, A. Aravind, V. Viasnoff, V. Studer, and J. B. Sibarita, “3D high- and super-resolution imaging using single-objective SPIM,” Nat. Methods 12(7), 641–644 (2015).
[Crossref] [PubMed]

Simão, D.

E. J. Gualda, D. Simão, C. Pinto, P. M. Alves, and C. Brito, “Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy,” Front. Cell. Neurosci. 8, 221 (2014).
[Crossref] [PubMed]

Solary, E.

B. Pruvot, A. Jacquel, N. Droin, P. Auberger, D. Bouscary, J. Tamburini, M. Muller, M. Fontenay, J. Chluba, and E. Solary, “Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy,” Haematologica 96(4), 612–616 (2011).
[Crossref] [PubMed]

Stelzer, E. H. K.

F. Pampaloni, B. J. Chang, and E. H. K. Stelzer, “Light sheet-based fluorescence microscopy (LSFM) for the quantitative imaging of cells and tissues,” Cell Tissue Res. 360(1), 129–141 (2015).
[Crossref] [PubMed]

F. Pampaloni, E. G. Reynaud, and E. H. K. Stelzer, “The third dimension bridges the gap between cell culture and live tissue,” Nat. Rev. Mol. Cell Biol. 8(10), 839–845 (2007).
[Crossref] [PubMed]

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4(4), 311–313 (2007).
[PubMed]

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Studer, V.

R. Galland, G. Grenci, A. Aravind, V. Viasnoff, V. Studer, and J. B. Sibarita, “3D high- and super-resolution imaging using single-objective SPIM,” Nat. Methods 12(7), 641–644 (2015).
[Crossref] [PubMed]

Stuurman, N.

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref] [PubMed]

Swoger, J.

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4(4), 311–313 (2007).
[PubMed]

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Tamburini, J.

B. Pruvot, A. Jacquel, N. Droin, P. Auberger, D. Bouscary, J. Tamburini, M. Muller, M. Fontenay, J. Chluba, and E. Solary, “Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy,” Haematologica 96(4), 612–616 (2011).
[Crossref] [PubMed]

Tomancak, P.

E. G. Reynaud, J. Peychl, J. Huisken, and P. Tomancak, “Guide to light-sheet microscopy for adventurous biologists,” Nat. Methods 12(1), 30–34 (2014).
[Crossref] [PubMed]

Tsuchida, M. A.

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref] [PubMed]

Vale, R. D.

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref] [PubMed]

Vale, T.

E. J. Gualda, T. Vale, P. Almada, J. A. Feijó, G. G. Martins, and N. Moreno, “OpenSpinMicroscopy: an open-source integrated microscopy platform,” Nat. Methods 10(7), 599–600 (2013).
[Crossref] [PubMed]

Verveer, P. J.

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4(4), 311–313 (2007).
[PubMed]

Viasnoff, V.

R. Galland, G. Grenci, A. Aravind, V. Viasnoff, V. Studer, and J. B. Sibarita, “3D high- and super-resolution imaging using single-objective SPIM,” Nat. Methods 12(7), 641–644 (2015).
[Crossref] [PubMed]

Wasserman, S. C.

C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010).
[Crossref] [PubMed]

Weber, M.

A. Kaufmann, M. Mickoleit, M. Weber, and J. Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139(17), 3242–3247 (2012).
[Crossref] [PubMed]

Wittbrodt, J.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Wittig, R.

T. Bruns, S. Schickinger, R. Wittig, and H. Schneckenburger, “Preparation strategy and illumination of three-dimensional cell cultures in light sheet-based fluorescence microscopy,” J. Biomed. Opt. 17(10), 1015181 (2012).
[Crossref] [PubMed]

Wu, J.

Wu, Y.

A. Kumar, Y. Wu, R. Christensen, P. Chandris, W. Gandler, E. McCreedy, A. Bokinsky, D. A. Colón-Ramos, Z. Bao, M. McAuliffe, G. Rondeau, and H. Shroff, “Dual-view plane illumination microscopy for rapid and spatially isotropic imaging,” Nat. Protoc. 9(11), 2555–2573 (2014).
[Crossref] [PubMed]

Yanik, M. F.

C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010).
[Crossref] [PubMed]

AIP Adv. (1)

R. Regmi, K. Mohan, and P. P. Mondal, “High resolution light-sheet based high-throughput imaging cytometry system enables visualization of intra-cellular organelles,” AIP Adv. 4(9), 097125 (2014).
[Crossref]

Arch. Toxicol. (1)

S. P. Rebelo, R. Costa, M. Estrada, V. Shevchenko, C. Brito, and P. M. Alves, “HepaRG microencapsulated spheroids in DMSO-free culture: novel culturing approaches for enhanced xenobiotic and biosynthetic metabolism,” Arch. Toxicol. 89, 1347–1358 (2014).
[PubMed]

Biomicrofluidics (1)

S. H. Cho, J. M. Godin, C. Chen, W. Qiao, H. Lee, and Y. Lo, “Recent advancements in optofluidicflow cytometer,” Biomicrofluidics 4(4), 043001 (2010).
[Crossref]

Birth Defects Res. C Embryo Today (1)

C. A. Lessman, “The developing zebrafish (danio rerio): a vertebrate model for high-throughput screening of chemical libraries,” Birth Defects Res. C Embryo Today 93(3), 268–280 (2011).
[Crossref] [PubMed]

Cell Tissue Res. (1)

F. Pampaloni, B. J. Chang, and E. H. K. Stelzer, “Light sheet-based fluorescence microscopy (LSFM) for the quantitative imaging of cells and tissues,” Cell Tissue Res. 360(1), 129–141 (2015).
[Crossref] [PubMed]

Development (1)

A. Kaufmann, M. Mickoleit, M. Weber, and J. Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139(17), 3242–3247 (2012).
[Crossref] [PubMed]

Front. Cell. Neurosci. (1)

E. J. Gualda, D. Simão, C. Pinto, P. M. Alves, and C. Brito, “Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy,” Front. Cell. Neurosci. 8, 221 (2014).
[Crossref] [PubMed]

Future Med. Chem. (1)

J. R. Mathias, M. T. Saxena, and J. S. Mumm, “Advances in zebrafish chemical screening technologies,” Future Med. Chem. 4(14), 1811–1822 (2012).
[Crossref] [PubMed]

Haematologica (1)

B. Pruvot, A. Jacquel, N. Droin, P. Auberger, D. Bouscary, J. Tamburini, M. Muller, M. Fontenay, J. Chluba, and E. Solary, “Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy,” Haematologica 96(4), 612–616 (2011).
[Crossref] [PubMed]

J. Biol. Methods (1)

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref] [PubMed]

J. Biomed. Opt. (1)

T. Bruns, S. Schickinger, R. Wittig, and H. Schneckenburger, “Preparation strategy and illumination of three-dimensional cell cultures in light sheet-based fluorescence microscopy,” J. Biomed. Opt. 17(10), 1015181 (2012).
[Crossref] [PubMed]

Nat. Methods (6)

P. J. Verveer, J. Swoger, F. Pampaloni, K. Greger, M. Marcello, and E. H. K. Stelzer, “High-resolution three-dimensional imaging of large specimens with light sheet-based microscopy,” Nat. Methods 4(4), 311–313 (2007).
[PubMed]

M. B. Ahrens, M. B. Orger, D. N. Robson, J. M. Li, and P. J. Keller, “Whole-brain functional imaging at cellular resolution using light-sheet microscopy,” Nat. Methods 10(5), 413–420 (2013).
[Crossref] [PubMed]

E. J. Gualda, T. Vale, P. Almada, J. A. Feijó, G. G. Martins, and N. Moreno, “OpenSpinMicroscopy: an open-source integrated microscopy platform,” Nat. Methods 10(7), 599–600 (2013).
[Crossref] [PubMed]

C. Pardo-Martin, T. Y. Chang, B. K. Koo, C. L. Gilleland, S. C. Wasserman, and M. F. Yanik, “High-throughput in vivo vertebrate screening,” Nat. Methods 7(8), 634–636 (2010).
[Crossref] [PubMed]

R. Galland, G. Grenci, A. Aravind, V. Viasnoff, V. Studer, and J. B. Sibarita, “3D high- and super-resolution imaging using single-objective SPIM,” Nat. Methods 12(7), 641–644 (2015).
[Crossref] [PubMed]

E. G. Reynaud, J. Peychl, J. Huisken, and P. Tomancak, “Guide to light-sheet microscopy for adventurous biologists,” Nat. Methods 12(1), 30–34 (2014).
[Crossref] [PubMed]

Nat. Protoc. (2)

A. Kumar, Y. Wu, R. Christensen, P. Chandris, W. Gandler, E. McCreedy, A. Bokinsky, D. A. Colón-Ramos, Z. Bao, M. McAuliffe, G. Rondeau, and H. Shroff, “Dual-view plane illumination microscopy for rapid and spatially isotropic imaging,” Nat. Protoc. 9(11), 2555–2573 (2014).
[Crossref] [PubMed]

J. Friedrich, C. Seidel, R. Ebner, and L. A. Kunz-Schughart, “Spheroid-based drug screen: considerations and practical approach,” Nat. Protoc. 4(3), 309–324 (2009).
[Crossref] [PubMed]

Nat. Rev. Drug Discov. (1)

P. Neuži, S. Giselbrecht, K. Länge, T. J. Huang, and A. Manz, “Revisiting lab-on-a-chip technology for drug discovery,” Nat. Rev. Drug Discov. 11(8), 620–632 (2012).
[Crossref] [PubMed]

Nat. Rev. Mol. Cell Biol. (1)

F. Pampaloni, E. G. Reynaud, and E. H. K. Stelzer, “The third dimension bridges the gap between cell culture and live tissue,” Nat. Rev. Mol. Cell Biol. 8(10), 839–845 (2007).
[Crossref] [PubMed]

Opt. Express (1)

Optica (1)

Science (1)

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Other (1)

M. F. Estrada, Biomaterials, Under submission (2015).

Supplementary Material (3)

NameDescription
» Visualization 1: AVI (22494 KB)      Demonstration of the microcapsules flow inside the FPE tube in both, brightfield (left) and dual color fluorescence mode (right)..
» Visualization 2: AVI (89064 KB)      Volume reconstruction of two Fli:GFP zebrafish larvae optained with double side detection F-SPIM (top); and stitching of four 3D stack using V-SPIM mode (bottom).
» Visualization 3: AVI (45574 KB)      Time lapse movie of the circulatory system development in two different Fli:GFP embryos.

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Figures (3)

Fig. 1
Fig. 1

(a) Schematic of the SPIM-Fluid set up. The different elements of the system are: lasers (L), dichroic mirrors (DM), filter wheels (FW), shutter (S), galvo mirror (GM), telescope (T), cylindrical lens (CL), cameras (CAM) and photo-detection system (PDS). In the basic system CAM 1 is used for the Flow-Mode and CAM 2 for the Vertical-Mode. Alternatively, CAM2 can be used for double side Flow-Mode or fast double color Flow-Mode are plotted in gray. The HT-sample management system consist on bottom rounded multiwall plates mounted on a XY stage, and a third motor controlling the FEP tip height. (b) Detailed scheme of the automatic sample mounting system based on an Arduino controlled injector with a photodiode system and Fluorinated Ethylene Propylene (FEP) tubes. (c) Front panel of the Micromanager SPIM-Fluid plugin. (d) Arduino controlled photodiode circuit for automatized sample positioning control. (e) Detailed scheme of the sample chamber, where the FEP tube crosses at 45°. Laser illumination is shown green and FEP tube in blue. For detailed info check our website.

Fig. 2
Fig. 2

(a-c) Maximum projection of three different 3D co-culture of cancer cell aggregates (green) and fibroblasts (magenta) entrapped in alginate microcapsules. Visualization 1 shows the microcapsules flow inside FEP tubes, in bright-field and fluorescence modes. The system allows the semi-automatic quantitative analysis of different parameters of the 3D co-cultures, such as (d) number of aggregates (n = 40 microcapsules; mean = 3.00; standard deviation (SD) = 2.15) (e) number of fibroblast per capsule (n = 40 microcapsules; mean = 489.90; SD = 126.70), (f) estimation of cell aggregate volumes (n = 120 cell aggregates; mean = 3.15 × 10−03; SD = 3.02 × 10−03) and (g) phenotypic classification of aggregates according to their circularity. The analysis was performed on a population of 40 microcapsules, produced and collected on the same conditions without any randomization or blinding process; only microcapsules without cancer cell aggregates were removed from the analysis. Scale bar: 200 µm.

Fig. 3
Fig. 3

Maximum projections of zebrafish embryos and larvae volume reconstructions: (a-c) three of the twelve Hras:GFP embryos at early developmental stages automatically loaded from a multi-well plate. 90° projection of an anesthetized Fli:GFP larvae acquired with F-Mode (d) single and (e) double side mode. (f) 6 day old Hras:GFP zebrafish imaged in F-Mode. (g) Same larvae imaged with V-Mode after stitching of seven 3D stacks. (h) 8 day Fli:GFP larvae acquired with V-Mode. The image is build up from four different 3D stacks. (i) Vascular system (green) of a 6-day fixed Fli:GFP larvae injected with human cancer cells, stained with Dil (magenta). The image was acquired using single side F-Mode. Sample courtesy of Dr. M. Ferreira and Dr. R. Fior. Volume reconstructions of Fli:GFP zebrafish are shown in Visualization 2 while Visualization 3 presents the development of the vascular system on Fli:GFP zebrafish using V-Mode. Scale bar: 200 microns.

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