Abstract

Near-infrared (NIR) fluorescence imaging systems have been developed for image guided surgery in recent years. However, current systems are typically bulky and work only when surgical light in the operating room (OR) is off. We propose a single camera imaging system that is capable of capturing NIR fluorescence and color images under normal surgical lighting illumination. Using a new RGB-NIR sensor and synchronized NIR excitation illumination, we have demonstrated that the system can acquire both color information and fluorescence signal with high sensitivity under normal surgical lighting illumination. The experimental results show that ICG sample with concentration of 0.13 μM can be detected when the excitation irradiance is 3.92 mW/cm2 at an exposure time of 10 ms.

© 2014 Optical Society of America

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    [CrossRef] [PubMed]

2014

2013

J. Glatz, J. Varga, P. B. Garcia-Allende, M. Koch, F. R. Greten, and V. Ntziachristos, “Concurrent video-rate color and near-infrared fluorescence laparoscopy,” J. Biomed. Opt.18(10), 101302 (2013).
[CrossRef] [PubMed]

K. Sexton, S. C. Davis, D. McClatchy, P. A. Valdes, S. C. Kanick, K. D. Paulsen, D. W. Roberts, and B. W. Pogue, “Pulsed-light imaging for fluorescence guided surgery under normal room lighting,” Opt. Lett.38(17), 3249–3252 (2013).
[CrossRef] [PubMed]

S. Keereweer, P. B. Van Driel, T. J. Snoeks, J. D. Kerrebijn, R. J. Baatenburg de Jong, A. L. Vahrmeijer, H. J. Sterenborg, and C. W. Löwik, “Optical image-guided cancer surgery: challenges and limitations,” Clin. Cancer Res.19(14), 3745–3754 (2013).
[CrossRef] [PubMed]

V. Venugopal, M. Park, Y. Ashitate, F. Neacsu, F. Kettenring, J. V. Frangioni, S. P. Gangadharan, and S. Gioux, “Design and characterization of an optimized simultaneous color and near-infrared fluorescence rigid endoscopic imaging system,” J. Biomed. Opt.18(12), 126018 (2013).
[CrossRef] [PubMed]

2012

E. M. Sevick-Muraca, “Translation of near-infrared fluorescence imaging technologies: emerging clinical applications,” Annu. Rev. Med.63(1), 217–231 (2012).
[CrossRef] [PubMed]

2011

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

2010

M. V. Marshall, J. C. Rasmussen, I. C. Tan, M. B. Aldrich, K. E. Adams, X. Wang, C. E. Fife, E. A. Maus, L. A. Smith, and E. M. Sevick-Muraca, “Near-infrared fluorescence imaging in humans with indocyanine green: a review and update,” Open Surg Oncol J2(2), 12–25 (2010).
[CrossRef] [PubMed]

2009

S. L. Troyan, V. Kianzad, S. L. Gibbs-Strauss, S. Gioux, A. Matsui, R. Oketokoun, L. Ngo, A. Khamene, F. Azar, and J. V. Frangioni, “The FLARE Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping,” Ann. Surg. Oncol.16(10), 2943–2952 (2009).
[CrossRef] [PubMed]

G. Themelis, J. S. Yoo, K. S. Soh, R. Schulz, and V. Ntziachristos, “Real-time intraoperative fluorescence imaging system using light-absorption correction,” J. Biomed. Opt.14(6), 064012 (2009).
[CrossRef] [PubMed]

T. Handa, R. G. Katare, S. Sasaguri, and T. Sato, “Preliminary experience for the evaluation of the intraoperative graft patency with real color charge-coupled device camera system: an advanced device for simultaneous capturing of color and near-infrared images during coronary artery bypass graft,” Interact. Cardiovasc. Thorac. Surg.9(2), 150–154 (2009).
[CrossRef] [PubMed]

2006

W. Stummer, U. Pichlmeier, T. Meinel, O. D. Wiestler, F. Zanella, H. J. Reulen, and ALA-Glioma Study Group, “Fluorescence-guided surgery with 5-aminolevulinic acid for resection of malignant glioma: a randomised controlled multicentre phase III trial,” Lancet Oncol.7(5), 392–401 (2006).
[CrossRef] [PubMed]

2004

M. Takahashi, T. Ishikawa, K. Higashidani, and H. Katoh, “SPY: an innovative intra-operative imaging system to evaluate graft patency during off-pump coronary artery bypass grafting,” Interact. Cardiovasc. Thorac. Surg.3(3), 479–483 (2004).
[CrossRef] [PubMed]

2003

J. V. Frangioni, “In vivo near-infrared fluorescence imaging,” Curr. Opin. Chem. Biol.7(5), 626–634 (2003).
[CrossRef] [PubMed]

2001

J. Lee and E. Sevick-Muraca, “Fluorescence-enhanced absorption imaging using frequency-domain photon migration: tolerance to measurement error,” J. Biomed. Opt.6(1), 58–67 (2001).
[CrossRef] [PubMed]

Adams, K. E.

M. V. Marshall, J. C. Rasmussen, I. C. Tan, M. B. Aldrich, K. E. Adams, X. Wang, C. E. Fife, E. A. Maus, L. A. Smith, and E. M. Sevick-Muraca, “Near-infrared fluorescence imaging in humans with indocyanine green: a review and update,” Open Surg Oncol J2(2), 12–25 (2010).
[CrossRef] [PubMed]

Aldrich, M. B.

M. V. Marshall, J. C. Rasmussen, I. C. Tan, M. B. Aldrich, K. E. Adams, X. Wang, C. E. Fife, E. A. Maus, L. A. Smith, and E. M. Sevick-Muraca, “Near-infrared fluorescence imaging in humans with indocyanine green: a review and update,” Open Surg Oncol J2(2), 12–25 (2010).
[CrossRef] [PubMed]

Arts, H. J.

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

Ashitate, Y.

V. Venugopal, M. Park, Y. Ashitate, F. Neacsu, F. Kettenring, J. V. Frangioni, S. P. Gangadharan, and S. Gioux, “Design and characterization of an optimized simultaneous color and near-infrared fluorescence rigid endoscopic imaging system,” J. Biomed. Opt.18(12), 126018 (2013).
[CrossRef] [PubMed]

Azar, F.

S. L. Troyan, V. Kianzad, S. L. Gibbs-Strauss, S. Gioux, A. Matsui, R. Oketokoun, L. Ngo, A. Khamene, F. Azar, and J. V. Frangioni, “The FLARE Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping,” Ann. Surg. Oncol.16(10), 2943–2952 (2009).
[CrossRef] [PubMed]

Baatenburg de Jong, R. J.

S. Keereweer, P. B. Van Driel, T. J. Snoeks, J. D. Kerrebijn, R. J. Baatenburg de Jong, A. L. Vahrmeijer, H. J. Sterenborg, and C. W. Löwik, “Optical image-guided cancer surgery: challenges and limitations,” Clin. Cancer Res.19(14), 3745–3754 (2013).
[CrossRef] [PubMed]

Bart, J.

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

Chen, Z.

Crane, L. M.

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

Davis, S. C.

de Jong, J. S.

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

Fife, C. E.

M. V. Marshall, J. C. Rasmussen, I. C. Tan, M. B. Aldrich, K. E. Adams, X. Wang, C. E. Fife, E. A. Maus, L. A. Smith, and E. M. Sevick-Muraca, “Near-infrared fluorescence imaging in humans with indocyanine green: a review and update,” Open Surg Oncol J2(2), 12–25 (2010).
[CrossRef] [PubMed]

Frangioni, J. V.

V. Venugopal, M. Park, Y. Ashitate, F. Neacsu, F. Kettenring, J. V. Frangioni, S. P. Gangadharan, and S. Gioux, “Design and characterization of an optimized simultaneous color and near-infrared fluorescence rigid endoscopic imaging system,” J. Biomed. Opt.18(12), 126018 (2013).
[CrossRef] [PubMed]

S. L. Troyan, V. Kianzad, S. L. Gibbs-Strauss, S. Gioux, A. Matsui, R. Oketokoun, L. Ngo, A. Khamene, F. Azar, and J. V. Frangioni, “The FLARE Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping,” Ann. Surg. Oncol.16(10), 2943–2952 (2009).
[CrossRef] [PubMed]

J. V. Frangioni, “In vivo near-infrared fluorescence imaging,” Curr. Opin. Chem. Biol.7(5), 626–634 (2003).
[CrossRef] [PubMed]

Gangadharan, S. P.

V. Venugopal, M. Park, Y. Ashitate, F. Neacsu, F. Kettenring, J. V. Frangioni, S. P. Gangadharan, and S. Gioux, “Design and characterization of an optimized simultaneous color and near-infrared fluorescence rigid endoscopic imaging system,” J. Biomed. Opt.18(12), 126018 (2013).
[CrossRef] [PubMed]

Garcia-Allende, P. B.

J. Glatz, J. Varga, P. B. Garcia-Allende, M. Koch, F. R. Greten, and V. Ntziachristos, “Concurrent video-rate color and near-infrared fluorescence laparoscopy,” J. Biomed. Opt.18(10), 101302 (2013).
[CrossRef] [PubMed]

Gibbs-Strauss, S. L.

S. L. Troyan, V. Kianzad, S. L. Gibbs-Strauss, S. Gioux, A. Matsui, R. Oketokoun, L. Ngo, A. Khamene, F. Azar, and J. V. Frangioni, “The FLARE Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping,” Ann. Surg. Oncol.16(10), 2943–2952 (2009).
[CrossRef] [PubMed]

Gioux, S.

V. Venugopal, M. Park, Y. Ashitate, F. Neacsu, F. Kettenring, J. V. Frangioni, S. P. Gangadharan, and S. Gioux, “Design and characterization of an optimized simultaneous color and near-infrared fluorescence rigid endoscopic imaging system,” J. Biomed. Opt.18(12), 126018 (2013).
[CrossRef] [PubMed]

S. L. Troyan, V. Kianzad, S. L. Gibbs-Strauss, S. Gioux, A. Matsui, R. Oketokoun, L. Ngo, A. Khamene, F. Azar, and J. V. Frangioni, “The FLARE Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping,” Ann. Surg. Oncol.16(10), 2943–2952 (2009).
[CrossRef] [PubMed]

Glatz, J.

J. Glatz, J. Varga, P. B. Garcia-Allende, M. Koch, F. R. Greten, and V. Ntziachristos, “Concurrent video-rate color and near-infrared fluorescence laparoscopy,” J. Biomed. Opt.18(10), 101302 (2013).
[CrossRef] [PubMed]

Greten, F. R.

J. Glatz, J. Varga, P. B. Garcia-Allende, M. Koch, F. R. Greten, and V. Ntziachristos, “Concurrent video-rate color and near-infrared fluorescence laparoscopy,” J. Biomed. Opt.18(10), 101302 (2013).
[CrossRef] [PubMed]

Handa, T.

T. Handa, R. G. Katare, S. Sasaguri, and T. Sato, “Preliminary experience for the evaluation of the intraoperative graft patency with real color charge-coupled device camera system: an advanced device for simultaneous capturing of color and near-infrared images during coronary artery bypass graft,” Interact. Cardiovasc. Thorac. Surg.9(2), 150–154 (2009).
[CrossRef] [PubMed]

Harlaar, N. J.

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

Higashidani, K.

M. Takahashi, T. Ishikawa, K. Higashidani, and H. Katoh, “SPY: an innovative intra-operative imaging system to evaluate graft patency during off-pump coronary artery bypass grafting,” Interact. Cardiovasc. Thorac. Surg.3(3), 479–483 (2004).
[CrossRef] [PubMed]

Ishikawa, T.

M. Takahashi, T. Ishikawa, K. Higashidani, and H. Katoh, “SPY: an innovative intra-operative imaging system to evaluate graft patency during off-pump coronary artery bypass grafting,” Interact. Cardiovasc. Thorac. Surg.3(3), 479–483 (2004).
[CrossRef] [PubMed]

Kanick, S. C.

Katare, R. G.

T. Handa, R. G. Katare, S. Sasaguri, and T. Sato, “Preliminary experience for the evaluation of the intraoperative graft patency with real color charge-coupled device camera system: an advanced device for simultaneous capturing of color and near-infrared images during coronary artery bypass graft,” Interact. Cardiovasc. Thorac. Surg.9(2), 150–154 (2009).
[CrossRef] [PubMed]

Katoh, H.

M. Takahashi, T. Ishikawa, K. Higashidani, and H. Katoh, “SPY: an innovative intra-operative imaging system to evaluate graft patency during off-pump coronary artery bypass grafting,” Interact. Cardiovasc. Thorac. Surg.3(3), 479–483 (2004).
[CrossRef] [PubMed]

Keereweer, S.

S. Keereweer, P. B. Van Driel, T. J. Snoeks, J. D. Kerrebijn, R. J. Baatenburg de Jong, A. L. Vahrmeijer, H. J. Sterenborg, and C. W. Löwik, “Optical image-guided cancer surgery: challenges and limitations,” Clin. Cancer Res.19(14), 3745–3754 (2013).
[CrossRef] [PubMed]

Kelder, W.

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

Kerrebijn, J. D.

S. Keereweer, P. B. Van Driel, T. J. Snoeks, J. D. Kerrebijn, R. J. Baatenburg de Jong, A. L. Vahrmeijer, H. J. Sterenborg, and C. W. Löwik, “Optical image-guided cancer surgery: challenges and limitations,” Clin. Cancer Res.19(14), 3745–3754 (2013).
[CrossRef] [PubMed]

Kettenring, F.

V. Venugopal, M. Park, Y. Ashitate, F. Neacsu, F. Kettenring, J. V. Frangioni, S. P. Gangadharan, and S. Gioux, “Design and characterization of an optimized simultaneous color and near-infrared fluorescence rigid endoscopic imaging system,” J. Biomed. Opt.18(12), 126018 (2013).
[CrossRef] [PubMed]

Khamene, A.

S. L. Troyan, V. Kianzad, S. L. Gibbs-Strauss, S. Gioux, A. Matsui, R. Oketokoun, L. Ngo, A. Khamene, F. Azar, and J. V. Frangioni, “The FLARE Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping,” Ann. Surg. Oncol.16(10), 2943–2952 (2009).
[CrossRef] [PubMed]

Kianzad, V.

S. L. Troyan, V. Kianzad, S. L. Gibbs-Strauss, S. Gioux, A. Matsui, R. Oketokoun, L. Ngo, A. Khamene, F. Azar, and J. V. Frangioni, “The FLARE Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping,” Ann. Surg. Oncol.16(10), 2943–2952 (2009).
[CrossRef] [PubMed]

Koch, M.

J. Glatz, J. Varga, P. B. Garcia-Allende, M. Koch, F. R. Greten, and V. Ntziachristos, “Concurrent video-rate color and near-infrared fluorescence laparoscopy,” J. Biomed. Opt.18(10), 101302 (2013).
[CrossRef] [PubMed]

Lee, J.

J. Lee and E. Sevick-Muraca, “Fluorescence-enhanced absorption imaging using frequency-domain photon migration: tolerance to measurement error,” J. Biomed. Opt.6(1), 58–67 (2001).
[CrossRef] [PubMed]

Liang, R.

Low, P. S.

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

Löwik, C. W.

S. Keereweer, P. B. Van Driel, T. J. Snoeks, J. D. Kerrebijn, R. J. Baatenburg de Jong, A. L. Vahrmeijer, H. J. Sterenborg, and C. W. Löwik, “Optical image-guided cancer surgery: challenges and limitations,” Clin. Cancer Res.19(14), 3745–3754 (2013).
[CrossRef] [PubMed]

Marshall, M. V.

M. V. Marshall, J. C. Rasmussen, I. C. Tan, M. B. Aldrich, K. E. Adams, X. Wang, C. E. Fife, E. A. Maus, L. A. Smith, and E. M. Sevick-Muraca, “Near-infrared fluorescence imaging in humans with indocyanine green: a review and update,” Open Surg Oncol J2(2), 12–25 (2010).
[CrossRef] [PubMed]

Matsui, A.

S. L. Troyan, V. Kianzad, S. L. Gibbs-Strauss, S. Gioux, A. Matsui, R. Oketokoun, L. Ngo, A. Khamene, F. Azar, and J. V. Frangioni, “The FLARE Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping,” Ann. Surg. Oncol.16(10), 2943–2952 (2009).
[CrossRef] [PubMed]

Maus, E. A.

M. V. Marshall, J. C. Rasmussen, I. C. Tan, M. B. Aldrich, K. E. Adams, X. Wang, C. E. Fife, E. A. Maus, L. A. Smith, and E. M. Sevick-Muraca, “Near-infrared fluorescence imaging in humans with indocyanine green: a review and update,” Open Surg Oncol J2(2), 12–25 (2010).
[CrossRef] [PubMed]

McClatchy, D.

Meinel, T.

W. Stummer, U. Pichlmeier, T. Meinel, O. D. Wiestler, F. Zanella, H. J. Reulen, and ALA-Glioma Study Group, “Fluorescence-guided surgery with 5-aminolevulinic acid for resection of malignant glioma: a randomised controlled multicentre phase III trial,” Lancet Oncol.7(5), 392–401 (2006).
[CrossRef] [PubMed]

Neacsu, F.

V. Venugopal, M. Park, Y. Ashitate, F. Neacsu, F. Kettenring, J. V. Frangioni, S. P. Gangadharan, and S. Gioux, “Design and characterization of an optimized simultaneous color and near-infrared fluorescence rigid endoscopic imaging system,” J. Biomed. Opt.18(12), 126018 (2013).
[CrossRef] [PubMed]

Ngo, L.

S. L. Troyan, V. Kianzad, S. L. Gibbs-Strauss, S. Gioux, A. Matsui, R. Oketokoun, L. Ngo, A. Khamene, F. Azar, and J. V. Frangioni, “The FLARE Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping,” Ann. Surg. Oncol.16(10), 2943–2952 (2009).
[CrossRef] [PubMed]

Ntziachristos, V.

J. Glatz, J. Varga, P. B. Garcia-Allende, M. Koch, F. R. Greten, and V. Ntziachristos, “Concurrent video-rate color and near-infrared fluorescence laparoscopy,” J. Biomed. Opt.18(10), 101302 (2013).
[CrossRef] [PubMed]

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

G. Themelis, J. S. Yoo, K. S. Soh, R. Schulz, and V. Ntziachristos, “Real-time intraoperative fluorescence imaging system using light-absorption correction,” J. Biomed. Opt.14(6), 064012 (2009).
[CrossRef] [PubMed]

Oketokoun, R.

S. L. Troyan, V. Kianzad, S. L. Gibbs-Strauss, S. Gioux, A. Matsui, R. Oketokoun, L. Ngo, A. Khamene, F. Azar, and J. V. Frangioni, “The FLARE Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping,” Ann. Surg. Oncol.16(10), 2943–2952 (2009).
[CrossRef] [PubMed]

Park, M.

V. Venugopal, M. Park, Y. Ashitate, F. Neacsu, F. Kettenring, J. V. Frangioni, S. P. Gangadharan, and S. Gioux, “Design and characterization of an optimized simultaneous color and near-infrared fluorescence rigid endoscopic imaging system,” J. Biomed. Opt.18(12), 126018 (2013).
[CrossRef] [PubMed]

Paulsen, K. D.

Pichlmeier, U.

W. Stummer, U. Pichlmeier, T. Meinel, O. D. Wiestler, F. Zanella, H. J. Reulen, and ALA-Glioma Study Group, “Fluorescence-guided surgery with 5-aminolevulinic acid for resection of malignant glioma: a randomised controlled multicentre phase III trial,” Lancet Oncol.7(5), 392–401 (2006).
[CrossRef] [PubMed]

Pleijhuis, R. G.

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

Pogue, B. W.

Rasmussen, J. C.

B. Zhu, J. C. Rasmussen, and E. M. Sevick-Muraca, “Non-invasive fluorescence imaging under ambient light conditions using a modulated ICCD and laser diode,” Biomed. Opt. Express5(2), 562–572 (2014).
[CrossRef] [PubMed]

M. V. Marshall, J. C. Rasmussen, I. C. Tan, M. B. Aldrich, K. E. Adams, X. Wang, C. E. Fife, E. A. Maus, L. A. Smith, and E. M. Sevick-Muraca, “Near-infrared fluorescence imaging in humans with indocyanine green: a review and update,” Open Surg Oncol J2(2), 12–25 (2010).
[CrossRef] [PubMed]

Reulen, H. J.

W. Stummer, U. Pichlmeier, T. Meinel, O. D. Wiestler, F. Zanella, H. J. Reulen, and ALA-Glioma Study Group, “Fluorescence-guided surgery with 5-aminolevulinic acid for resection of malignant glioma: a randomised controlled multicentre phase III trial,” Lancet Oncol.7(5), 392–401 (2006).
[CrossRef] [PubMed]

Roberts, D. W.

Sarantopoulos, A.

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

Sasaguri, S.

T. Handa, R. G. Katare, S. Sasaguri, and T. Sato, “Preliminary experience for the evaluation of the intraoperative graft patency with real color charge-coupled device camera system: an advanced device for simultaneous capturing of color and near-infrared images during coronary artery bypass graft,” Interact. Cardiovasc. Thorac. Surg.9(2), 150–154 (2009).
[CrossRef] [PubMed]

Sato, T.

T. Handa, R. G. Katare, S. Sasaguri, and T. Sato, “Preliminary experience for the evaluation of the intraoperative graft patency with real color charge-coupled device camera system: an advanced device for simultaneous capturing of color and near-infrared images during coronary artery bypass graft,” Interact. Cardiovasc. Thorac. Surg.9(2), 150–154 (2009).
[CrossRef] [PubMed]

Schulz, R.

G. Themelis, J. S. Yoo, K. S. Soh, R. Schulz, and V. Ntziachristos, “Real-time intraoperative fluorescence imaging system using light-absorption correction,” J. Biomed. Opt.14(6), 064012 (2009).
[CrossRef] [PubMed]

Sevick-Muraca, E.

J. Lee and E. Sevick-Muraca, “Fluorescence-enhanced absorption imaging using frequency-domain photon migration: tolerance to measurement error,” J. Biomed. Opt.6(1), 58–67 (2001).
[CrossRef] [PubMed]

Sevick-Muraca, E. M.

B. Zhu, J. C. Rasmussen, and E. M. Sevick-Muraca, “Non-invasive fluorescence imaging under ambient light conditions using a modulated ICCD and laser diode,” Biomed. Opt. Express5(2), 562–572 (2014).
[CrossRef] [PubMed]

E. M. Sevick-Muraca, “Translation of near-infrared fluorescence imaging technologies: emerging clinical applications,” Annu. Rev. Med.63(1), 217–231 (2012).
[CrossRef] [PubMed]

M. V. Marshall, J. C. Rasmussen, I. C. Tan, M. B. Aldrich, K. E. Adams, X. Wang, C. E. Fife, E. A. Maus, L. A. Smith, and E. M. Sevick-Muraca, “Near-infrared fluorescence imaging in humans with indocyanine green: a review and update,” Open Surg Oncol J2(2), 12–25 (2010).
[CrossRef] [PubMed]

Sexton, K.

Smith, L. A.

M. V. Marshall, J. C. Rasmussen, I. C. Tan, M. B. Aldrich, K. E. Adams, X. Wang, C. E. Fife, E. A. Maus, L. A. Smith, and E. M. Sevick-Muraca, “Near-infrared fluorescence imaging in humans with indocyanine green: a review and update,” Open Surg Oncol J2(2), 12–25 (2010).
[CrossRef] [PubMed]

Snoeks, T. J.

S. Keereweer, P. B. Van Driel, T. J. Snoeks, J. D. Kerrebijn, R. J. Baatenburg de Jong, A. L. Vahrmeijer, H. J. Sterenborg, and C. W. Löwik, “Optical image-guided cancer surgery: challenges and limitations,” Clin. Cancer Res.19(14), 3745–3754 (2013).
[CrossRef] [PubMed]

Soh, K. S.

G. Themelis, J. S. Yoo, K. S. Soh, R. Schulz, and V. Ntziachristos, “Real-time intraoperative fluorescence imaging system using light-absorption correction,” J. Biomed. Opt.14(6), 064012 (2009).
[CrossRef] [PubMed]

Sterenborg, H. J.

S. Keereweer, P. B. Van Driel, T. J. Snoeks, J. D. Kerrebijn, R. J. Baatenburg de Jong, A. L. Vahrmeijer, H. J. Sterenborg, and C. W. Löwik, “Optical image-guided cancer surgery: challenges and limitations,” Clin. Cancer Res.19(14), 3745–3754 (2013).
[CrossRef] [PubMed]

Stummer, W.

W. Stummer, U. Pichlmeier, T. Meinel, O. D. Wiestler, F. Zanella, H. J. Reulen, and ALA-Glioma Study Group, “Fluorescence-guided surgery with 5-aminolevulinic acid for resection of malignant glioma: a randomised controlled multicentre phase III trial,” Lancet Oncol.7(5), 392–401 (2006).
[CrossRef] [PubMed]

Takahashi, M.

M. Takahashi, T. Ishikawa, K. Higashidani, and H. Katoh, “SPY: an innovative intra-operative imaging system to evaluate graft patency during off-pump coronary artery bypass grafting,” Interact. Cardiovasc. Thorac. Surg.3(3), 479–483 (2004).
[CrossRef] [PubMed]

Tan, I. C.

M. V. Marshall, J. C. Rasmussen, I. C. Tan, M. B. Aldrich, K. E. Adams, X. Wang, C. E. Fife, E. A. Maus, L. A. Smith, and E. M. Sevick-Muraca, “Near-infrared fluorescence imaging in humans with indocyanine green: a review and update,” Open Surg Oncol J2(2), 12–25 (2010).
[CrossRef] [PubMed]

Themelis, G.

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

G. Themelis, J. S. Yoo, K. S. Soh, R. Schulz, and V. Ntziachristos, “Real-time intraoperative fluorescence imaging system using light-absorption correction,” J. Biomed. Opt.14(6), 064012 (2009).
[CrossRef] [PubMed]

Troyan, S. L.

S. L. Troyan, V. Kianzad, S. L. Gibbs-Strauss, S. Gioux, A. Matsui, R. Oketokoun, L. Ngo, A. Khamene, F. Azar, and J. V. Frangioni, “The FLARE Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping,” Ann. Surg. Oncol.16(10), 2943–2952 (2009).
[CrossRef] [PubMed]

Vahrmeijer, A. L.

S. Keereweer, P. B. Van Driel, T. J. Snoeks, J. D. Kerrebijn, R. J. Baatenburg de Jong, A. L. Vahrmeijer, H. J. Sterenborg, and C. W. Löwik, “Optical image-guided cancer surgery: challenges and limitations,” Clin. Cancer Res.19(14), 3745–3754 (2013).
[CrossRef] [PubMed]

Valdes, P. A.

van Dam, G. M.

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

van der Zee, A. G.

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

Van Driel, P. B.

S. Keereweer, P. B. Van Driel, T. J. Snoeks, J. D. Kerrebijn, R. J. Baatenburg de Jong, A. L. Vahrmeijer, H. J. Sterenborg, and C. W. Löwik, “Optical image-guided cancer surgery: challenges and limitations,” Clin. Cancer Res.19(14), 3745–3754 (2013).
[CrossRef] [PubMed]

Varga, J.

J. Glatz, J. Varga, P. B. Garcia-Allende, M. Koch, F. R. Greten, and V. Ntziachristos, “Concurrent video-rate color and near-infrared fluorescence laparoscopy,” J. Biomed. Opt.18(10), 101302 (2013).
[CrossRef] [PubMed]

Venugopal, V.

V. Venugopal, M. Park, Y. Ashitate, F. Neacsu, F. Kettenring, J. V. Frangioni, S. P. Gangadharan, and S. Gioux, “Design and characterization of an optimized simultaneous color and near-infrared fluorescence rigid endoscopic imaging system,” J. Biomed. Opt.18(12), 126018 (2013).
[CrossRef] [PubMed]

Wang, X.

Z. Chen, X. Wang, and R. Liang, “RGB-NIR multispectral camera,” Opt. Express22(5), 4985–4994 (2014).
[CrossRef] [PubMed]

M. V. Marshall, J. C. Rasmussen, I. C. Tan, M. B. Aldrich, K. E. Adams, X. Wang, C. E. Fife, E. A. Maus, L. A. Smith, and E. M. Sevick-Muraca, “Near-infrared fluorescence imaging in humans with indocyanine green: a review and update,” Open Surg Oncol J2(2), 12–25 (2010).
[CrossRef] [PubMed]

Wiestler, O. D.

W. Stummer, U. Pichlmeier, T. Meinel, O. D. Wiestler, F. Zanella, H. J. Reulen, and ALA-Glioma Study Group, “Fluorescence-guided surgery with 5-aminolevulinic acid for resection of malignant glioma: a randomised controlled multicentre phase III trial,” Lancet Oncol.7(5), 392–401 (2006).
[CrossRef] [PubMed]

Yoo, J. S.

G. Themelis, J. S. Yoo, K. S. Soh, R. Schulz, and V. Ntziachristos, “Real-time intraoperative fluorescence imaging system using light-absorption correction,” J. Biomed. Opt.14(6), 064012 (2009).
[CrossRef] [PubMed]

Zanella, F.

W. Stummer, U. Pichlmeier, T. Meinel, O. D. Wiestler, F. Zanella, H. J. Reulen, and ALA-Glioma Study Group, “Fluorescence-guided surgery with 5-aminolevulinic acid for resection of malignant glioma: a randomised controlled multicentre phase III trial,” Lancet Oncol.7(5), 392–401 (2006).
[CrossRef] [PubMed]

Zhu, B.

Ann. Surg. Oncol.

S. L. Troyan, V. Kianzad, S. L. Gibbs-Strauss, S. Gioux, A. Matsui, R. Oketokoun, L. Ngo, A. Khamene, F. Azar, and J. V. Frangioni, “The FLARE Intraoperative Near-Infrared Fluorescence Imaging System: A First-in-Human Clinical Trial in Breast Cancer Sentinel Lymph Node Mapping,” Ann. Surg. Oncol.16(10), 2943–2952 (2009).
[CrossRef] [PubMed]

Annu. Rev. Med.

E. M. Sevick-Muraca, “Translation of near-infrared fluorescence imaging technologies: emerging clinical applications,” Annu. Rev. Med.63(1), 217–231 (2012).
[CrossRef] [PubMed]

Biomed. Opt. Express

Clin. Cancer Res.

S. Keereweer, P. B. Van Driel, T. J. Snoeks, J. D. Kerrebijn, R. J. Baatenburg de Jong, A. L. Vahrmeijer, H. J. Sterenborg, and C. W. Löwik, “Optical image-guided cancer surgery: challenges and limitations,” Clin. Cancer Res.19(14), 3745–3754 (2013).
[CrossRef] [PubMed]

Curr. Opin. Chem. Biol.

J. V. Frangioni, “In vivo near-infrared fluorescence imaging,” Curr. Opin. Chem. Biol.7(5), 626–634 (2003).
[CrossRef] [PubMed]

Interact. Cardiovasc. Thorac. Surg.

M. Takahashi, T. Ishikawa, K. Higashidani, and H. Katoh, “SPY: an innovative intra-operative imaging system to evaluate graft patency during off-pump coronary artery bypass grafting,” Interact. Cardiovasc. Thorac. Surg.3(3), 479–483 (2004).
[CrossRef] [PubMed]

T. Handa, R. G. Katare, S. Sasaguri, and T. Sato, “Preliminary experience for the evaluation of the intraoperative graft patency with real color charge-coupled device camera system: an advanced device for simultaneous capturing of color and near-infrared images during coronary artery bypass graft,” Interact. Cardiovasc. Thorac. Surg.9(2), 150–154 (2009).
[CrossRef] [PubMed]

J. Biomed. Opt.

G. Themelis, J. S. Yoo, K. S. Soh, R. Schulz, and V. Ntziachristos, “Real-time intraoperative fluorescence imaging system using light-absorption correction,” J. Biomed. Opt.14(6), 064012 (2009).
[CrossRef] [PubMed]

J. Lee and E. Sevick-Muraca, “Fluorescence-enhanced absorption imaging using frequency-domain photon migration: tolerance to measurement error,” J. Biomed. Opt.6(1), 58–67 (2001).
[CrossRef] [PubMed]

J. Glatz, J. Varga, P. B. Garcia-Allende, M. Koch, F. R. Greten, and V. Ntziachristos, “Concurrent video-rate color and near-infrared fluorescence laparoscopy,” J. Biomed. Opt.18(10), 101302 (2013).
[CrossRef] [PubMed]

V. Venugopal, M. Park, Y. Ashitate, F. Neacsu, F. Kettenring, J. V. Frangioni, S. P. Gangadharan, and S. Gioux, “Design and characterization of an optimized simultaneous color and near-infrared fluorescence rigid endoscopic imaging system,” J. Biomed. Opt.18(12), 126018 (2013).
[CrossRef] [PubMed]

Lancet Oncol.

W. Stummer, U. Pichlmeier, T. Meinel, O. D. Wiestler, F. Zanella, H. J. Reulen, and ALA-Glioma Study Group, “Fluorescence-guided surgery with 5-aminolevulinic acid for resection of malignant glioma: a randomised controlled multicentre phase III trial,” Lancet Oncol.7(5), 392–401 (2006).
[CrossRef] [PubMed]

Nat. Med.

G. M. van Dam, G. Themelis, L. M. Crane, N. J. Harlaar, R. G. Pleijhuis, W. Kelder, A. Sarantopoulos, J. S. de Jong, H. J. Arts, A. G. van der Zee, J. Bart, P. S. Low, and V. Ntziachristos, “Intraoperative tumor-specific fluorescence imaging in ovarian cancer by folate receptor-α targeting: first in-human results,” Nat. Med.17(10), 1315–1319 (2011).
[CrossRef] [PubMed]

Open Surg Oncol J

M. V. Marshall, J. C. Rasmussen, I. C. Tan, M. B. Aldrich, K. E. Adams, X. Wang, C. E. Fife, E. A. Maus, L. A. Smith, and E. M. Sevick-Muraca, “Near-infrared fluorescence imaging in humans with indocyanine green: a review and update,” Open Surg Oncol J2(2), 12–25 (2010).
[CrossRef] [PubMed]

Opt. Express

Opt. Lett.

Other

http://www.fluoptics.com/fluoptics_Fluorescence_Imaging_System.php

http://www.iht-ltd.com/pde-photodynamic-eye/

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Figures (7)

Fig. 1
Fig. 1

Schematic diagram of the experimental setup.

Fig. 2
Fig. 2

(a) Color filter arrays with four bandpass filters in RGB-NIR camera and (b) spectral sensitivity of the color filter arrays [17].

Fig. 3
Fig. 3

Spectra of surgical light with the normal fluorescent room light on/off. The inset (a) is the NIR spectrum difference with the fluorescent room light on/off and the inset (b) is the NIR spectrum of the surgical light between 700 nm and 900 nm.

Fig. 4
Fig. 4

Raw and extracted images of the USAF 1951 test target under visible and NIR (850 nm) illumination. (a) Raw image, (b) image extracted from Green channel, and (c) image extracted from NIR channel

Fig. 5
Fig. 5

(a) NIR image of ICG samples under normal surgical lighting condition; (b) NIR image of ICG samples under the surgical lighting condition with excitation light on; (c) Difference image between (a) and (b). The concentration of ICG from left to right are 10.32 μM, 5.16 μM, 2.58 μM, 1.29 μM, 0.65 μM, 0.32 μM, 0.13 μM and 0 μM respectively.

Fig. 6
Fig. 6

(a) ICG sensitivity curve plot, showing the fluorescence signal intensity versus ICG concentration and NIR excitation power, and (b) SNR versus ICG concentration and NIR excitation power.

Fig. 7
Fig. 7

(a) RGB image of chicken breast with excitation light off. (b) RGB image with excitation light on. (c) NIR image with excitation light off. (d) NIR image with excitation light on. (e) Difference image between (c) and (d). (f) Difference image displayed in false color. (g) Merged image of the difference image (f) and color image (a). (h) Merged image with different color map and threshold.

Equations (1)

Equations on this page are rendered with MathJax. Learn more.

SN R dB =20 log 10 ( S RMSN ),

Metrics