Abstract

In this study, the light diffraction of temporal focusing multiphoton excitation microscopy (TFMPEM) and the excitation patterning of nonlinear structured-illumination microscopy (NSIM) can be simultaneously and accurately implemented via a single high-resolution digital micromirror device. The lateral and axial spatial resolutions of the TFMPEM are remarkably improved through the second-order NSIM and projected structured light, respectively. The experimental results demonstrate that the lateral and axial resolutions are enhanced from 397 nm to 168 nm (2.4-fold) and from 2.33 μm to 1.22 μm (1.9-fold), respectively, in full width at the half maximum. Furthermore, a three-dimensionally rendered image of a cytoskeleton cell featuring ~25 nm microtubules is improved, with other microtubules at a distance near the lateral resolution of 168 nm also able to be distinguished.

© 2014 Optical Society of America

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2014 (1)

2013 (6)

H. Choi, E. Y. S. Yew, B. Hallacoglu, S. Fantini, C. J. R. Sheppard, and P. T. C. So, “Improvement of axial resolution and contrast in temporally focused widefield two-photon microscopy with structured light illumination,” Biomed. Opt. Express4(7), 995–1005 (2013).
[CrossRef] [PubMed]

K. Isobe, T. Takeda, K. Mochizuki, Q. Song, A. Suda, F. Kannari, H. Kawano, A. Kumagai, A. Miyawaki, and K. Midorikawa, “Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination,” Biomed. Opt. Express4(11), 2396–2410 (2013).
[CrossRef] [PubMed]

E. E. Hoover and J. A. Squier, “Advances in multiphoton microscopy technology,” Nat. Photonics7(2), 93–101 (2013).
[CrossRef] [PubMed]

K. Weisshart, T. Dertinger, T. Kalkbrenner, I. Kleppe, and M. Kempe, “Super-resolution microscopy heads towards 3D dynamics,” Adv. Opt. Technol.2, 211–231 (2013).

P. T. C. So, E. Y. S. Yew, and C. Rowlands, “High-Throughput Nonlinear Optical Microscopy,” Biophys. J.105(12), 2641–2654 (2013).
[CrossRef] [PubMed]

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, “High-throughput fabrication of gray-level bio-microstructures via temporal focusing excitation and laser pulse control,” J. Biomed. Opt.18(7), 075004 (2013).
[CrossRef]

2012 (5)

2011 (4)

S. Geissbuehler, C. Dellagiacoma, and T. Lasser, “Comparison between SOFI and STORM,” Biomed. Opt. Express2(3), 408–420 (2011).
[CrossRef] [PubMed]

O. D. Therrien, B. Aubé, S. Pagès, P. D. Koninck, and D. Côté, “Wide-field multiphoton imaging of cellular dynamics in thick tissue by temporal focusing and patterned illumination,” Biomed. Opt. Express2(3), 696–704 (2011).
[CrossRef] [PubMed]

J. Mertz, “Optical sectioning microscopy with planar or structured illumination,” Nat. Methods8(10), 811–819 (2011).
[CrossRef] [PubMed]

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

2010 (2)

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

D. Kim and P. T. C. So, “High-throughput three-dimensional lithographic microfabrication,” Opt. Lett.35(10), 1602–1604 (2010).
[CrossRef] [PubMed]

2009 (1)

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A.106(52), 22287–22292 (2009).
[CrossRef] [PubMed]

2008 (3)

M. G. Gustafsson, L. Shao, P. M. Carlton, C. J. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J.94(12), 4957–4970 (2008).
[CrossRef] [PubMed]

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A.105(51), 20221–20226 (2008).
[CrossRef] [PubMed]

D. Lim, K. K. Chu, and J. Mertz, “Wide-field fluorescence sectioning with hybrid speckle and uniform-illumination microscopy,” Opt. Lett.33(16), 1819–1821 (2008).
[CrossRef] [PubMed]

2006 (3)

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

S. T. Hess, T. P. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods3(10), 793–796 (2006).
[CrossRef] [PubMed]

2005 (3)

2000 (2)

M. G. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc.198(2), 82–87 (2000).
[CrossRef] [PubMed]

M. G. Gustafsson, D. A. Agard, and J. W. Sedat, “Doubling the lateral resolution of wide-field fluorescence microscopy using structured illumination,” Proc. SPIE3919, 141–150 (2000).
[CrossRef]

1997 (1)

Agard, D. A.

M. G. Gustafsson, L. Shao, P. M. Carlton, C. J. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J.94(12), 4957–4970 (2008).
[CrossRef] [PubMed]

M. G. Gustafsson, D. A. Agard, and J. W. Sedat, “Doubling the lateral resolution of wide-field fluorescence microscopy using structured illumination,” Proc. SPIE3919, 141–150 (2000).
[CrossRef]

Anselmi, F.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

Aubé, B.

Bates, M.

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods3(10), 793–796 (2006).
[CrossRef] [PubMed]

Bègue, A.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

Betzig, E.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Blake, G. A.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Bonifacino, J. S.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Campagnola, P. J.

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, “High-throughput fabrication of gray-level bio-microstructures via temporal focusing excitation and laser pulse control,” J. Biomed. Opt.18(7), 075004 (2013).
[CrossRef]

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-H. Lien, P. J. Campagnola, and S.-J. Chen, “Fast multiphoton microfabrication of freeform polymer microstructures by spatiotemporal focusing and patterned excitation,” Opt. Express20(17), 19030–19038 (2012).
[CrossRef] [PubMed]

Cande, W. Z.

M. G. Gustafsson, L. Shao, P. M. Carlton, C. J. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J.94(12), 4957–4970 (2008).
[CrossRef] [PubMed]

Carlton, P. M.

M. G. Gustafsson, L. Shao, P. M. Carlton, C. J. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J.94(12), 4957–4970 (2008).
[CrossRef] [PubMed]

Chang, C.-Y.

Chang, N.-S.

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, “High-throughput fabrication of gray-level bio-microstructures via temporal focusing excitation and laser pulse control,” J. Biomed. Opt.18(7), 075004 (2013).
[CrossRef]

L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express20(8), 8939–8948 (2012).
[CrossRef] [PubMed]

Chen, S.-J.

Chen, Y.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Cheng, L.-C.

Cho, K.-C.

Choi, H.

Chu, K. K.

Colyer, R.

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A.106(52), 22287–22292 (2009).
[CrossRef] [PubMed]

Côté, D.

Dana, H.

Davidson, M. W.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

de Sars, V.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

Dellagiacoma, C.

Dertinger, T.

K. Weisshart, T. Dertinger, T. Kalkbrenner, I. Kleppe, and M. Kempe, “Super-resolution microscopy heads towards 3D dynamics,” Adv. Opt. Technol.2, 211–231 (2013).

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A.106(52), 22287–22292 (2009).
[CrossRef] [PubMed]

Dong, C. Y.

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, “High-throughput fabrication of gray-level bio-microstructures via temporal focusing excitation and laser pulse control,” J. Biomed. Opt.18(7), 075004 (2013).
[CrossRef]

L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express20(8), 8939–8948 (2012).
[CrossRef] [PubMed]

Durst, M.

Emiliani, V.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

Enderlein, J.

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A.106(52), 22287–22292 (2009).
[CrossRef] [PubMed]

Fajardo, O.

P. Zhu, O. Fajardo, J. Shum, Y.-P. Zhang Schärer, and R. W. Friedrich, “High-resolution optical control of spatiotemporal neuronal activity patterns in zebrafish using a digital micromirror device,” Nat. Protoc.7(7), 1410–1425 (2012).
[CrossRef] [PubMed]

Fantini, S.

Ford, T. N.

T. N. Ford, D. Lim, and J. Mertz, “Fast optically sectioned fluorescence HiLo endomicroscopy,” J. Biomed. Opt.17(2), 021105 (2012).
[CrossRef] [PubMed]

Friedrich, R. W.

P. Zhu, O. Fajardo, J. Shum, Y.-P. Zhang Schärer, and R. W. Friedrich, “High-resolution optical control of spatiotemporal neuronal activity patterns in zebrafish using a digital micromirror device,” Nat. Protoc.7(7), 1410–1425 (2012).
[CrossRef] [PubMed]

Geissbuehler, S.

Girirajan, T. P.

S. T. Hess, T. P. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

Glückstad, J.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

Golubovskaya, I. N.

M. G. Gustafsson, L. Shao, P. M. Carlton, C. J. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J.94(12), 4957–4970 (2008).
[CrossRef] [PubMed]

Guo, C.-L.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Gustafsson, M. G.

M. G. Gustafsson, L. Shao, P. M. Carlton, C. J. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J.94(12), 4957–4970 (2008).
[CrossRef] [PubMed]

M. G. Gustafsson, “Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution,” Proc. Natl. Acad. Sci. U.S.A.102(37), 13081–13086 (2005).
[CrossRef] [PubMed]

M. G. Gustafsson, D. A. Agard, and J. W. Sedat, “Doubling the lateral resolution of wide-field fluorescence microscopy using structured illumination,” Proc. SPIE3919, 141–150 (2000).
[CrossRef]

M. G. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc.198(2), 82–87 (2000).
[CrossRef] [PubMed]

Hallacoglu, B.

Hess, H. F.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Hess, S. T.

S. T. Hess, T. P. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

Holland, D. B.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Hoover, E. E.

E. E. Hoover and J. A. Squier, “Advances in multiphoton microscopy technology,” Nat. Photonics7(2), 93–101 (2013).
[CrossRef] [PubMed]

Hu, Y. Y.

Isacoff, E. Y.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

Isobe, K.

Iyer, G.

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A.106(52), 22287–22292 (2009).
[CrossRef] [PubMed]

Juskaitis, R.

Kalkbrenner, T.

K. Weisshart, T. Dertinger, T. Kalkbrenner, I. Kleppe, and M. Kempe, “Super-resolution microscopy heads towards 3D dynamics,” Adv. Opt. Technol.2, 211–231 (2013).

Kannari, F.

Kawano, H.

Kempe, M.

K. Weisshart, T. Dertinger, T. Kalkbrenner, I. Kleppe, and M. Kempe, “Super-resolution microscopy heads towards 3D dynamics,” Adv. Opt. Technol.2, 211–231 (2013).

Kim, D.

Kleppe, I.

K. Weisshart, T. Dertinger, T. Kalkbrenner, I. Kleppe, and M. Kempe, “Super-resolution microscopy heads towards 3D dynamics,” Adv. Opt. Technol.2, 211–231 (2013).

Koninck, P. D.

Kumagai, A.

Kuo, C.-H.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Lasser, T.

Li, Y.-C.

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, “High-throughput fabrication of gray-level bio-microstructures via temporal focusing excitation and laser pulse control,” J. Biomed. Opt.18(7), 075004 (2013).
[CrossRef]

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-H. Lien, P. J. Campagnola, and S.-J. Chen, “Fast multiphoton microfabrication of freeform polymer microstructures by spatiotemporal focusing and patterned excitation,” Opt. Express20(17), 19030–19038 (2012).
[CrossRef] [PubMed]

Lien, C. H.

Lien, C.-H.

Lim, D.

Lin, C.-Y.

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, “High-throughput fabrication of gray-level bio-microstructures via temporal focusing excitation and laser pulse control,” J. Biomed. Opt.18(7), 075004 (2013).
[CrossRef]

L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express20(8), 8939–8948 (2012).
[CrossRef] [PubMed]

Lindwasser, O. W.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Lippincott-Schwartz, J.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Mason, M. D.

S. T. Hess, T. P. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

Mertz, J.

T. N. Ford, D. Lim, and J. Mertz, “Fast optically sectioned fluorescence HiLo endomicroscopy,” J. Biomed. Opt.17(2), 021105 (2012).
[CrossRef] [PubMed]

J. Mertz, “Optical sectioning microscopy with planar or structured illumination,” Nat. Methods8(10), 811–819 (2011).
[CrossRef] [PubMed]

D. Lim, K. K. Chu, and J. Mertz, “Wide-field fluorescence sectioning with hybrid speckle and uniform-illumination microscopy,” Opt. Lett.33(16), 1819–1821 (2008).
[CrossRef] [PubMed]

Midorikawa, K.

Miyawaki, A.

Mochizuki, K.

Neil, M. A. A.

Olenych, S.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Oron, D.

Ouyang, M.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Pagès, S.

Papagiakoumou, E.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

Patterson, G. H.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Rowlands, C.

P. T. C. So, E. Y. S. Yew, and C. Rowlands, “High-Throughput Nonlinear Optical Microscopy,” Biophys. J.105(12), 2641–2654 (2013).
[CrossRef] [PubMed]

Rust, M. J.

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods3(10), 793–796 (2006).
[CrossRef] [PubMed]

Sedat, J. W.

M. G. Gustafsson, L. Shao, P. M. Carlton, C. J. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J.94(12), 4957–4970 (2008).
[CrossRef] [PubMed]

M. G. Gustafsson, D. A. Agard, and J. W. Sedat, “Doubling the lateral resolution of wide-field fluorescence microscopy using structured illumination,” Proc. SPIE3919, 141–150 (2000).
[CrossRef]

Shank, C. V.

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A.105(51), 20221–20226 (2008).
[CrossRef] [PubMed]

Shao, L.

M. G. Gustafsson, L. Shao, P. M. Carlton, C. J. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J.94(12), 4957–4970 (2008).
[CrossRef] [PubMed]

Sheppard, C. J. R.

Shoham, S.

Shroff, H.

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A.105(51), 20221–20226 (2008).
[CrossRef] [PubMed]

Shum, J.

P. Zhu, O. Fajardo, J. Shum, Y.-P. Zhang Schärer, and R. W. Friedrich, “High-resolution optical control of spatiotemporal neuronal activity patterns in zebrafish using a digital micromirror device,” Nat. Protoc.7(7), 1410–1425 (2012).
[CrossRef] [PubMed]

Sie, Y. D.

Silberberg, Y.

So, P. T. C.

Song, Q.

Sougrat, R.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

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E. E. Hoover and J. A. Squier, “Advances in multiphoton microscopy technology,” Nat. Photonics7(2), 93–101 (2013).
[CrossRef] [PubMed]

Suda, A.

Takeda, T.

Tal, E.

Tang, J.

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A.105(51), 20221–20226 (2008).
[CrossRef] [PubMed]

Therrien, O. D.

van Howe, J.

Vaziri, A.

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A.105(51), 20221–20226 (2008).
[CrossRef] [PubMed]

Wang, C. J.

M. G. Gustafsson, L. Shao, P. M. Carlton, C. J. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J.94(12), 4957–4970 (2008).
[CrossRef] [PubMed]

Weiss, S.

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A.106(52), 22287–22292 (2009).
[CrossRef] [PubMed]

Weisshart, K.

K. Weisshart, T. Dertinger, T. Kalkbrenner, I. Kleppe, and M. Kempe, “Super-resolution microscopy heads towards 3D dynamics,” Adv. Opt. Technol.2, 211–231 (2013).

Wilson, T.

Xu, C.

Yen, W.-C.

Yew, E. Y. S.

Yih, J. N.

Yu, J.-Y.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Zadoyan, R.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Zhang Schärer, Y.-P.

P. Zhu, O. Fajardo, J. Shum, Y.-P. Zhang Schärer, and R. W. Friedrich, “High-resolution optical control of spatiotemporal neuronal activity patterns in zebrafish using a digital micromirror device,” Nat. Protoc.7(7), 1410–1425 (2012).
[CrossRef] [PubMed]

Zhu, G.

Zhu, P.

P. Zhu, O. Fajardo, J. Shum, Y.-P. Zhang Schärer, and R. W. Friedrich, “High-resolution optical control of spatiotemporal neuronal activity patterns in zebrafish using a digital micromirror device,” Nat. Protoc.7(7), 1410–1425 (2012).
[CrossRef] [PubMed]

Zhuang, X.

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods3(10), 793–796 (2006).
[CrossRef] [PubMed]

Zipfel, W.

Adv. Opt. Technol. (1)

K. Weisshart, T. Dertinger, T. Kalkbrenner, I. Kleppe, and M. Kempe, “Super-resolution microscopy heads towards 3D dynamics,” Adv. Opt. Technol.2, 211–231 (2013).

Biomed. Opt. Express (4)

Biophys. J. (3)

P. T. C. So, E. Y. S. Yew, and C. Rowlands, “High-Throughput Nonlinear Optical Microscopy,” Biophys. J.105(12), 2641–2654 (2013).
[CrossRef] [PubMed]

M. G. Gustafsson, L. Shao, P. M. Carlton, C. J. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J.94(12), 4957–4970 (2008).
[CrossRef] [PubMed]

S. T. Hess, T. P. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91(11), 4258–4272 (2006).
[CrossRef] [PubMed]

J. Biomed. Opt. (3)

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, “High-throughput fabrication of gray-level bio-microstructures via temporal focusing excitation and laser pulse control,” J. Biomed. Opt.18(7), 075004 (2013).
[CrossRef]

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

T. N. Ford, D. Lim, and J. Mertz, “Fast optically sectioned fluorescence HiLo endomicroscopy,” J. Biomed. Opt.17(2), 021105 (2012).
[CrossRef] [PubMed]

J. Microsc. (1)

M. G. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc.198(2), 82–87 (2000).
[CrossRef] [PubMed]

Nat. Methods (3)

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Methods3(10), 793–796 (2006).
[CrossRef] [PubMed]

J. Mertz, “Optical sectioning microscopy with planar or structured illumination,” Nat. Methods8(10), 811–819 (2011).
[CrossRef] [PubMed]

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

Nat. Photonics (1)

E. E. Hoover and J. A. Squier, “Advances in multiphoton microscopy technology,” Nat. Photonics7(2), 93–101 (2013).
[CrossRef] [PubMed]

Nat. Protoc. (1)

P. Zhu, O. Fajardo, J. Shum, Y.-P. Zhang Schärer, and R. W. Friedrich, “High-resolution optical control of spatiotemporal neuronal activity patterns in zebrafish using a digital micromirror device,” Nat. Protoc.7(7), 1410–1425 (2012).
[CrossRef] [PubMed]

Opt. Express (4)

Opt. Lett. (5)

Proc. Natl. Acad. Sci. U.S.A. (3)

T. Dertinger, R. Colyer, G. Iyer, S. Weiss, and J. Enderlein, “Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI),” Proc. Natl. Acad. Sci. U.S.A.106(52), 22287–22292 (2009).
[CrossRef] [PubMed]

M. G. Gustafsson, “Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution,” Proc. Natl. Acad. Sci. U.S.A.102(37), 13081–13086 (2005).
[CrossRef] [PubMed]

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A.105(51), 20221–20226 (2008).
[CrossRef] [PubMed]

Proc. SPIE (1)

M. G. Gustafsson, D. A. Agard, and J. W. Sedat, “Doubling the lateral resolution of wide-field fluorescence microscopy using structured illumination,” Proc. SPIE3919, 141–150 (2000).
[CrossRef]

Science (1)

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313(5793), 1642–1645 (2006).
[CrossRef] [PubMed]

Other (1)

B. R. Masters and P. T. C. So, Handbook of Biomedical Nonlinear Optical Microscopy (Oxford, 2008).

Supplementary Material (2)

» Media 1: MOV (283 KB)     
» Media 2: MOV (277 KB)     

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Figures (5)

Fig. 1
Fig. 1

Schematic of the TFMPEM with incorporated DMD. The red dashed lines represent the mutual image-conjugate planes between the DMD surface and the objective lens’ focal plane.

Fig. 2
Fig. 2

(a) Sinusoidal illumination on the R6B fluorescent thin film. Blue circles: the detected fluorescent intensity; blue line: the fitting by [ 1+cos( k p r ) ] 2 . (b) Fourier transform of the detected structured pattern in Fig. 2(a), in which a nonlinear second-order peak caused by the intrinsic nonlinear TPE is indicated.

Fig. 3
Fig. 3

TPE images of 100 nm fluorescent beads by (a) TFMPEM only and (b) TFMPEM with second-order NSIM. (c) and (d) are the regions within red dashed circles in Figs. 3(a) and 3(b), respectively.

Fig. 4
Fig. 4

(a) The 100nm fluorescent beads’ measured lateral profiles by TFMPEM alone (blue) and NSIM enhanced TFMPEM (red). The lateral resolution with TFMPEM only and NSIM enhanced TFMPEM are 397 nm and 168 nm, respectively, in FWHM. (b) The R6G thin film’s measured excitation volume axial profile by TFMPEM (black), and the 200 nm fluorescent beads’ measured axial profiles by only TFMPEM (blue) and NSIM enhanced TFMPEM (red). The excitation volume depth is 3.1 μm in FWHM, and the axial resolution with only TFMPEM and NSIM enhanced TFMPEM are 2.33 μm and 1.22 μm, respectively, in FWHM. The circles represent the measured intensities at different lateral and axial positions, while the dashed lines are the fitted curves.

Fig. 5
Fig. 5

Cytoskeleton TPE fluorescence images by (a) the TFMPEM only (Media 1), and (b) the second-order NSIM enhanced TFMPEM (Media 2). (c) Intensity profiles along the intersecting red dashed line in Figs. 5(a) and 5(b). Blue line: from Fig. 5(a), red line: from Fig. 5(b).

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

f( r )c( r )× I 2 ( r )h( r ),
I 2 ( r )= { I 0 [ 1+cos( k p r +ϕ) ] } 2 ,
F( k )=aH( k ){ 3 2 C( k )+C( k + k p ) e jϕ +C( k k p ) e jϕ + 1 4 C( k +2 k p ) e j2ϕ + 1 4 C( k 2 k p ) e j2ϕ },

Metrics